Re: [Histonet] Input on Sakura E150 Purchase
I used one about 5 years ago with minimal issues. The only problem was finding parts to fix it. On one occasion we had to have someone fabricate a part to repair the unit. Other than that it worked well and was easy to maintain. Hope that helps Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7800 h...@histologistics.com On Wed, Feb 26, 2014 at 4:56 PM, margaret chadwell wrote: > > > > > > > > Sent from Windows Mail > > Hello Histonet, > > Does anyone have input on the Sukura E150 tissue processor? Our research lab > is looking at purchasing a used model from Rankin Biomedical, but we were > concerned about the age of the unit, 17 years old. They mentioned it is > totally refurbished, will give a one year warranty, say it's a reliable unit, > and parts are available for servicing. > > Any info or suggestions would be greatly appreciated. Like so many we are on > a limited budget so we can't buy new. Thank you in advance! > > Margaret Chadwell > > LIAI > > La Jolla, Ca > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Input on Sakura E150 Purchase
I have a VIP 300 from 1998 and it's still going strong. We have had a few repairs over the years the most recent having to replace the pump, its 16 years old and still functioning fine. As far as I am concerned it’s a reliable unit, we use it frequently. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of margaret chadwell Sent: Wednesday, February 26, 2014 2:57 PM To: histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] Input on Sakura E150 Purchase Sent from Windows Mail Hello Histonet, Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it’s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can’t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla, Ca ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Input on Sakura E150 Purchase
Sent from Windows Mail Hello Histonet, Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it’s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can’t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla, Ca___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Equipment recommendations, please
The Thermo Shandon Finesse is my favorite microtome. Wish I had one at my lab... I've never really had a preference for embedding consoles. As long as it's ambidextrous it's fine with me, I'm a lefty. -- Tony Auge HTL (ASCP) QIHC Histology Supervisor - Chandler Pathology Services Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Poly Scientific Paraffin
Afternoon Histoneters, I was just wondering if anyone is having issues with the paraffin from Poly Scientific? Our last shipment, the pellets look a lot smaller and it's not melting at the 60 degrees we originally had it programed! Vendor is recommending we increase the tempuerature 2 degrees. Is anyone else having issues with this paraffin? Thanks! Cecilia M. Obregon Memorial Regional Hospital 3501 Johnson Street Hollywood, FL 33021 CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] manual tissue processing protocol
Hello, I need some input on a manual tissue processing protocol I have received. (5 columns: reagents; tiny to large tissues...) Reagents\embryoes\small tissue\medium tissue\large tissue 70% EtOH\30 min--1hr\1 hr--ON\2hr--ON\4hr--ON 95% EtOH\30 min--1hr\1 hr\1hr\2hr 95% EtOH\30 min\30 min\1 hr\1 hr 100% EtOH\30 min\1 hr\30 min\1 hr 100% EtOH\10 min\30 min\30 min\1 hr toluene\30 min\1 hr\1hr\1 hr toluene\none\30 min\30 min\1 hr till clear parafin infiltration\1hr all parafin infiltration\1 hr\1 hr\2hr\2hr EM400\1 hr\1hr\1hr\2+ hr I would be replacing the toluene with methylbenzoate. Are there any anticipated issues? Is this a good protocol so far as producing good quality tissue for sectioning and analysis? I am not sure what is meant by "small" and "large" tissue. My fish are about 5 cm in length and about 1 cm thick. Would they be classed as small? Is it necessary using two different types of paraffin : Surgipath infiltrating paraffin Cat # 01400 (4-5 lb/bag) and Surgipath embedding paraffin? For the manual infiltration: would a beaker of paraffin on a 60oC hotplate and stirrer (on low) be OK? I would be fixing with PFA and decalcidying with 30% EDTA. Might refixing in the PFA after decal be a good idea to make sure the tissue integrity is maintained? I would probably stored fixed, decalsified samples in 70% EtOH until I can process fully. Any one think there would be complications from doing this? Thanks ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leasing an Electron Microscope
James: Depending upon the amount of EM work your have - another option is to "lease" scoping time from another facility in your area that maintains an EM scope. My lab pays for scoping time on a per hour basis. You of course have to work with the owner of the scope for availability but it works out well for us (and we don't have to maintain anything). Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital & Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, February 26, 2014 12:27 PM To: 'Vickroy, Jim' Cc: Histonet Subject: [Histonet] RE: Leasing an Electron Microscope James, even if a particular instrument company does not, there are leasing companies that specialize in setting up lease agreements for just about anything. For example, a quick search found this one. I haven't used any, but some labs use such companies when they don't have the capital budget for something. http://www.telelease.com/asset-finance/scientific-equipment-leasing Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, February 26, 2014 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leasing an Electron Microscope Anybody know of any Transmission Electron Microscope Companies that lease Transmission Electron Microscopes? If so please contact me. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leasing an Electron Microscope
James, even if a particular instrument company does not, there are leasing companies that specialize in setting up lease agreements for just about anything. For example, a quick search found this one. I haven't used any, but some labs use such companies when they don't have the capital budget for something. http://www.telelease.com/asset-finance/scientific-equipment-leasing Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, February 26, 2014 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leasing an Electron Microscope Anybody know of any Transmission Electron Microscope Companies that lease Transmission Electron Microscopes? If so please contact me. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slide Storage
CAP regs Anatomic Pathology ANP.23700 Storage Organization Under the notes section it states "The storage area for blocks should be cool to prevent blocks from melting together. Cytology CYP.017100 Slide Storage Under the notes section it states, "Cytopathology slides should be stored at room temperature for optimal preservation. Room temperature is often approximated at 20°C or 70°F. In scientific contexts, it usually denotes a range between 20 and 23.5 °C (68.0 and 74.3 °F) with an average of 21 °C (70 °F). Some instrumentation, such as your IHC instrument may have a specific Room Temp designation. Obviously, this is another measurement that must be recorded for CAP. And as far as I know, there are no national regulations requiring different retention times for peds records, but states could vary. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 2. Block/Slide retention for peds cases (Amber McKenzie) On Feb 25, 2014, at 5:45 PM, Amber McKenzie wrote: >Are there any regulations specifying any difference of holding blocks/slides >for peds cases? Are they treated the same as adults cases and held 10-12 >years or are they needed to be kept longer? In the block/slide storage room, are there any regulations on temp or humidity? Thanks! * - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leasing an Electron Microscope
Anybody know of any Transmission Electron Microscope Companies that lease Transmission Electron Microscopes? If so please contact me. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Email list
>From the Histonet guidelines when one subscribes: If you ever want to unsubscribe or change your options (eg, switch to or from digest mode, change your password, etc.), visit your subscription page at: http://lists.utsouthwestern.edu/mailman/options/histonet/shive003%40umn.edu -- Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu On Tue, Feb 25, 2014 at 5:27 PM, Stephanie Moore wrote: > Good Afternoon, > > I would like to know how I can be taken off the email list for Histonet. > > Thank you, > > > *Stephanie Huff, B.S., HTL(ASCP)CM* > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Equipment recommendations, please
Leica for Microtomy*, thermo histostar for embedding 26 Şub 2014 18:53 tarihinde "Mehmet Fatih BOZKURT" yazdı: > Leica for Microsoft, histostar for embedding. > 26 Şub 2014 17:58 tarihinde yazdı: > >> >> Which microtomes and embedding centers are you using out there? I am >> looking at the Leica RM2265 (since we're a research lab) and the Thermo >> Scientific Finesse (We have one and I like it!), but I am also open to >> suggestions. >> >> Thank you so much! >> Kathleen >> >> Principal Lab Technician >> Neurotoxicology Labs >> Molecular Pathology Facility Core >> Dept of Pharmacology & Toxicology >> Rutgers, the State University of NJ >> 41 B Gordon Road >> Piscataway, NJ 08854 >> (848) 445-1443 >> FAX (732) 445-6905 >> >> ___ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Gross only
Using the code 88300 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Wednesday, February 26, 2014 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross only How does everyone bill for "Gross Only"? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message, and any included attachments, are from Virtua Health or its related affiliates and is intended only for the addressee(s). The information contained herein is privileged, proprietary or may include confidential information and/or protected patient health information. Any unauthorized review, forwarding, printing, copying, distributing, or otherwise disseminating or taking any action based on such information is strictly prohibited. If you have received this message in error, or have reason to believe you are not authorized to receive it, please delete this message promptly and notify the sender by e-mail with a copy to issecur...@virtua.org. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Gross only
It should be 88300 Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya [tanyaabb...@catholichealth.net] Sent: Wednesday, February 26, 2014 11:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gross only How does everyone bill for "Gross Only"? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Gross only
do you mean the code? 2014= 88300 Joelle Weaver MAOM, HTL (ASCP) QIHC > From: tanyaabb...@catholichealth.net > To: histonet@lists.utsouthwestern.edu > Date: Wed, 26 Feb 2014 16:36:42 + > Subject: [Histonet] Gross only > > How does everyone bill for "Gross Only"? > Thanks! > > Tanya G. Abbott RT (CSMLS) > Manager Technologist, Histology/Cytology > St. Joseph Medical Center > Reading, PA 19603-0316 > ph 610-378-2635 > fax 610-898-5871 > email: tanyaabb...@catholichealth.net > > This electronic mail and any attached documents are intended solely for the > named addressee(s) and contain confidential information. If you are not an > addressee, or responsible for delivering this email to an addressee, you have > received this email in error and are notified that reading, copying, or > disclosing this email is prohibited. If you received this email in error, > immediately reply to the sender and delete the message completely from your > computer system. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Gross only
How does everyone bill for "Gross Only"? Thanks! Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Equipment recommendations, please
Which microtomes and embedding centers are you using out there? I am looking at the Leica RM2265 (since we're a research lab) and the Thermo Scientific Finesse (We have one and I like it!), but I am also open to suggestions. Thank you so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology & Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BRCA1 testing by IHC
Asking this question from our molecular lab: We're trying to find a lab that does BRCA1 testing by IHC (not sequencing) Can you post this on HistoNet and see if anyone knows who does this? The reagent exists, so someone must be using it. Thanks In advance Cindy DeRiso Yale University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HT Academic Position in Orlando, FL
Good Morning! Keiser University is seeking a Histotechnology Clinical Coordinator at their Orlando campus. For more info visit http://keiseruniversityevergladesuniversity.hodesiq.com/job_detail.asp?JobID=4257983&user_id= Thanks, Komal Komal Gada, BS, HT/HTL(ASCP), QIHC Histotechnology University Department Chair Histotechnology Program Director - Orlando Keiser University 5600 Lake Underhill Rd. Orlando, FL 32807 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
