[Histonet] GMS better than PAS for Fungi

[Tony Henwood (SCHN)]

Thought that the subject title should be changed.
(References available on request)

No,
Using Periodic acid instead of chromic acid just gives you a PASM. 
Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative.
The literature is quite confusing on pseudo-fungi. Some say that they are GMS 
positive whereas other claim they are GMS negative. 
My own experience is that they are GMS (using chromic acid) negative. 
It is possible that our pathologists aren't aware that the "GMS" that their lab 
does might be using Periodic acid instead of Chromic acid. 
Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of 
chromic acid.
The literature often does not report the exact GMS used which makes clear 
understanding of the histochemical results difficult.

Pneumocystis will not be easy to see unless chromic acid is used (the mucin 
stains strongly PAS (and hence PASM) positive obscuring the small 
microorganisms).

Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas 
they stain quite well with GMS.


Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory 
Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, 
School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead 
NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Thursday, 5 June 2014 5:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Acid Clean Glassware

Chromic acid does a nifty job of removing metal deposits on glassware, but so 
do many commercial lab detergents.
Chromic acid is the oxidizer for the fungus in the GMS stain.  Go one better 
and get rid of Chromic Acid out of your lab.  It is probably one of the more 
toxic / nasty chemicals in your department.  Instead, try Churukian's 
Ammoniacal Silver for Fungus in the microwave.  It is a much simpler, faster, 
prettier stain.  It uses Periodic Acid as the oxidizer and does not stain the 
elastic fibers like a regular GMS.  Both you and your pathologists will love 
it, I promise.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
Today's Topics:

   1. Acid Cleaned glassware (Abbott, Tanya)
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[Histonet] RE: Acid Clean Glassware

[Tony Henwood (SCHN)]

No,
Using Periodic acid instead of chromic acid just gives you a PASM. 
Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative.
The literature is quite confusing on pseudo-fungi. Some say that they are GMS 
positive whereas other claim they are GMS negative. 
My own experience is that they are GMS (using chromic acid) negative. 
It is possible that our pathologists aren't aware that the "GMS" that their lab 
does might be using Periodic acid instead of Chromic acid. 
Commercial kits (eg Sigma and Richard-Allan) use periodic acid instead of 
chromic acid.
The literature often does not report the exact GMS used which makes clear 
understanding of the histochemical results difficult.

Pneumocystis will not be easy to see unless chromic acid is used (the mucin 
stains strongly PAS (and hence PASM) positive obscuring the small 
microorganisms).

Using PAS, Old fungi, Mucor, Actinomyces and Nocordia do not stain well whereas 
they stain quite well with GMS.


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Thursday, 5 June 2014 5:58 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Acid Clean Glassware

Chromic acid does a nifty job of removing metal deposits on glassware, but so 
do many commercial lab detergents.
Chromic acid is the oxidizer for the fungus in the GMS stain.  Go one better 
and get rid of Chromic Acid out of your lab.  It is probably one of the more 
toxic / nasty chemicals in your department.  Instead, try Churukian's 
Ammoniacal Silver for Fungus in the microwave.  It is a much simpler, faster, 
prettier stain.  It uses Periodic Acid as the oxidizer and does not stain the 
elastic fibers like a regular GMS.  Both you and your pathologists will love 
it, I promise.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
Today's Topics:

   1. Acid Cleaned glassware (Abbott, Tanya)
-



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[Histonet] Validating slides

[Sharon Scalise]

I am looking for comments about validating slides in histology/immuno.  We have 
always validated our charged slides if we went to a new brand/vendor in order 
to ensure that the tissue sections will adhere properly during immuno 
procedures and certain special stains.  Once we found them to be acceptable, we 
continue to use them, lot to lot, and never validate as long as we do not 
change brand/vendor.  We recently ran into a problem with slides that we 
received by accident (similar packaging, different slide) and they were used 
for some immuno cases.  The sections on many of the slides fell off and the 
stains had to be repeated.  We realized the problem and pulled the slides out 
of service.  We contacted the vendor and they said that those slides should 
have been pulled from the shelf, that it was a bad batch and they should have 
never been sent to anyone, yet alone incorrectly sent to us.
I started to think that we might want to consider validating new lots we 
receive to ensure that each lot is acceptable.  As we began this discussion 
there were people who felt that in any lot you could have slides that are 
acceptable and maybe some boxes that give you problems.  How do you know if all 
slides in one lot are treated exactly the same?  Can you really use lot number 
to verify quality when it comes to slides?  I am not exactly sure of the 
process used to "charge" the slides so I don't feel qualified to answer these 
questions.
What is everyone else doing about slide validation?  Is it really necessary? (I 
can say that in 30 years I have never seen it done with each new lot, but I 
have been at the same job for the past 18 years).

Thanks for your input!

Sharon Scalise, HTL(ASCP)
Histology Supervisor-Anatomic Pathology
Beaumont Health System
3601 W. 13 Mile Rd.
Royal Oak, MI 48073
248 898-5981
sscal...@beaumont.edu


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[Histonet] Alpha Histotech - leaving the world of histology

[Victoria Baker]

Hi Alpha,

I've been
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[Histonet] RE: Acid Clean Glassware

[Terri Braud]

Chromic acid does a nifty job of removing metal deposits on glassware,
but so do many commercial lab detergents.
Chromic acid is the oxidizer for the fungus in the GMS stain.  Go one
better and get rid of Chromic Acid out of your lab.  It is probably one
of the more toxic / nasty chemicals in your department.  Instead, try
Churukian's Ammoniacal Silver for Fungus in the microwave.  It is a much
simpler, faster, prettier stain.  It uses Periodic Acid as the oxidizer
and does not stain the elastic fibers like a regular GMS.  Both you and
your pathologists will love it, I promise.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
Today's Topics:

   1. Acid Cleaned glassware (Abbott, Tanya)
-



CONFIDENTIALITY NOTICE:

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it was sent. It may contain information that is privileged and/or confidential,
and the use or disclosure of such information may also be restricted under 
applicable
federal and state law. If you received this communication in error, please do 
not
distribute any part of it or retain any copies, and delete the original E-Mail.
Please notify the sender of any error by E-Mail.

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[Histonet] How to ask a question on Histonet?

[Epp, Carol SktnHR]

I would like to ask a question:Headline-MICROSCOPIC CHECKS- "What slides do you 
microscopically check before sending out to the pathologist?" 
carol@saskatoonhealthregion.ca

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, June 04, 2014 11:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 127, Issue 5

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
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When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


Today's Topics:

   1. HTL program (Bilger, Andrea)
   2. RE: Histonet Digest, Vol 127, Issue 3 (Nancy Schmitt)
   3. Re: Should I leave histology world (Rene J Buesa)
   4. Re: fluorescent scope and slide scanner (Rene J Buesa)
   5. Clearium? (Abbott, Tanya)
   6. Pathologists? (Gudrun Lang)
   7. Re: Should I leave histology world (Emily Brown)
   8. RE: Should I leave histology world (Morken, Timothy)
   9. RE: fluorescent scope and slide scanner (Elizabeth Chlipala)
  10. HER2 by IHC- Fixation  (Adesupo, Adesuyi (Banjo))
  11. RE: HER2 by IHC- Fixation (Weems, Joyce K.)
  12. leaving histology question research is still an option
  (koelli...@comcast.net)


--

Message: 1
Date: Wed, 4 Jun 2014 14:33:24 +
From: "Bilger, Andrea" 
Subject: [Histonet] HTL program
To: "histonet@lists.utsouthwestern.edu"

Message-ID:

<0258e6074f274d4c81fd07cc80e8b...@by2pr02mb300.namprd02.prod.outlook.com>

Content-Type: text/plain; charset="us-ascii"

I have several techs who used Indiana University's on line course and they are 
all great techs!  Have not been impressed with techs from Harford Community's 
program.

Andrea Bilger, HTL
Team Leader, Histology
York Hospital
1001 S. George St.
York, Pa.  17405
(717) 851-5040


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Message: 2
Date: Wed, 4 Jun 2014 14:42:16 +
From: Nancy Schmitt 
Subject: [Histonet] RE: Histonet Digest, Vol 127, Issue 3
To: "histonet@lists.utsouthwestern.edu"

Message-ID:
<906b4da90ed1db4db6c7e94d7cee6c36d9cad...@peitha.wad.pa-ucl.com>
Content-Type: text/plain; charset="us-ascii"

Hi Denise-
I would HIGHLY recommend the online program at IUPUI - we have had 4 people go 
through this program including myself.
When finished you are ready to sit for the test! It is really well done and 
they are so helpful along the way.
Contact info: Debra M. Wood, Director,
 Histotechnology Program
 Clinical Assistant Professor
 Indiana University School of Medicine
 Department of Pathology & Laboratory Medicine
 350 W. 11th St. ROOM 6002A
 Indianapolis, IN 46202
 Program Office Phone: 317-491-6410

Good Luck!
Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA



_
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Denise
Sent: Tuesday, June 03, 2014 12:18 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] HTL Online Courses?

Hi all!

I have been doing massive research on this for past several days but no solid 
leads.  I am wondering about HT/HTL Histology online courses... I live in St. 
Louis Missouri and they don't offer any histology courses around here - only 
out of states.  I cannot relocate or do 1-2 years program out of state due to 
my full-time job and family reason.

Do anyone know any good Histology online courses that I can take without 
conflicting with my job?

Greatly appericated!  Thanks!

Denise Smith

smit...@kids.wustl.edu

[Histonet] RE: microglia antibodies

[Elizabeth Chlipala]

What species are you looking to stain?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiousis, Sam
Sent: Wednesday, June 04, 2014 12:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] microglia antibodies


I have been trying to get anitbodies for microglia (CD68, CD11b, IBA-1) to work 
using IHC and IF (regular, co-localization, even TSA amplification) on slides 
from frozen tissue that has been fixed in PFA and embedded in OCT with mixed 
crappy results.  Does anyone have any suggestions or protocols?

Thanks in advance

Sam Kiousis
Karmanos Cancer Institute
skiou...@med.wayne.edu

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RE: [Histonet] Practice tissue?

[Anne Murvosh]

Contact your local labs and Hospitals, you could just use some already
embedded and cut tissue that is about to be disposed of as long as there
is no patient info on the blocks it shouldn't be a problem.  Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of David
Waugh
Sent: Tuesday, June 03, 2014 2:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Practice tissue?

Hello histonet, I was a member a number of years ago, but had gotten out
of doing much histology (I was never a pro) for awhile but I seem to
be back at it. 
I was wondering if people might have some ideas on a good "practice
tissue", one that I could embed, section, and stain (H&E) to both
practice my sectioning, and check my staining protocol, etc. I really
need to be sectioning some decalcified teeth, and have had some luck,
but I would really like to do some more practice on an "easy to section
tissue" (other than just a blank block of paraffin)  that I can also
test my staining on.  I have accesses to a tissue processor. Is there
anything I can just pick up at the grocery store? Thanks, David
David A. Waugh, Ph.D.
Research Assistant
Department of Anatomy and Neurobiology
NEOMED
4209 State Route 44
Rootstown, OH 44272


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[Histonet] microglia antibodies

[Kiousis, Sam]

I have been trying to get anitbodies for microglia (CD68, CD11b, IBA-1) to work 
using IHC and IF (regular, co-localization, even TSA amplification) on slides 
from frozen tissue that has been fixed in PFA and embedded in OCT with mixed 
crappy results.  Does anyone have any suggestions or protocols?

Thanks in advance

Sam Kiousis
Karmanos Cancer Institute
skiou...@med.wayne.edu

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[Histonet] Great Job Opprotunity

[Jackie Pitts]

Hello all!
I am posting a great opportunity for a confident and experienced histotech. It 
is located in Baxter, MN. You would be working for Dr. Lundstrom at Dermatology 
Professionals.  The job is to for the most part run the histology lab. Right 
now this is my job and I am leaving only because I found a job nearer to my 
family.
You need to have experience in histology as you will be working alone and need 
to be able to handle the day to day situations. Grossing experience is also 
required as you will be grossing the skin shaves, punches and excisions. You 
will then be processing, embedding, cutting and staining the slides.  Must also 
be ASCP certified. MOHS experience would be a plus too, but not required.
Please contact me with a resume or any questions regarding this position. I am 
trying to help them get going on replacing me before I leave them at the end of 
the month. :)

Jackie Heitz, HT(ASCP)CM

218-454-3520
Dermatology Professionals, PA
Baxter, MN 56425

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[Histonet] CAP Question CYP.04300 Daily QC

[Piche, Jessica]

Hi Everyone,

I am asking a question for our Cytology department about  CYP.04300 on the CAP 
checklist, in regards to daily QC.  It states the technical quality of the 
preparations should be checked daily and includes checking all stains for 
predicted staining characteristics each day of use. This check should include 
preparations performed in the lab such as cytospins, cell blocks, and liquid 
based automated preparations.

Is the question asking to document evidence of the technical quality of the 
stain or the technical quality in the preparation of the slide, ie smear, 
cytospin, etc? Or does it want both? Our cytology department uses several 
different protocols  and with several different slide preparations so they are 
wondering if they are supposed to run a control every day for every single type 
of slide prep or just every stain that is used?

We would be interested in knowing what everyone else does.

Thank you and have a great day!

Jessica Piche,HT(ASCP)
Waterbury Hospital



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[Histonet] RE: IHC patient reports

[Morken, Timothy]

The patient results are in the final report. The 2 year requirement is for QC 
documents only.

Tim Morken
Supervisor,Hisotlogy,  Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
aobrie...@comcast.net
Sent: Wednesday, June 04, 2014 11:39 AM
To: histonet
Subject: [Histonet] IHC patient reports

How long is everyone keeping the IHC reports that are printed off after the run 
is completed? ANP.22660 states that Batch control records must be retained to 2 
years. Is that also the patient? 

Thanks 
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[Histonet] IHC patient reports

[aobrien88]

How long is everyone keeping the IHC reports that are printed off after the run 
is completed? ANP.22660 states that Batch control records must be retained to 2 
years. Is that also the patient? 

Thanks 
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[Histonet] RE: slide drying/curing before filing

[Weems, Joyce K.]

We use quick drying Richard Allen  Mounting Media  - 22 050 102, purchased 
through Fisher Healthcare.

We file the next day!! Glass coverslips...

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake, Kim S
Sent: Wednesday, June 04, 2014 1:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] slide drying/curing before filing

According to the Histonet archives, this hasn't been discussed for almost a 
decade.  How are labs drying/curing their slides before they are filed?

We are a small oral pathology laboratory and we hand-coverslip using Richard 
Allen mounting medium and glass coverslips.  After cases are signed out slides 
are placed in their cardboard folders in a 37C oven for at least a week, then 
are filed in metal slides drawers.

This works perfectly well, but we would like to simplify this, if possible.  A 
search of the archive showed a great deal of variation in slide drying 
protocol, I'm interested in hearing what everyone else is up to.  Of course, 
I'm hoping to get lots of people saying that they file all of last week's room 
temp slides on Monday morning and it works like a charm!

Thanks!

Kim Lake, MLS (ASCP)CM
Laboratory Manager
Oral Pathology Laboratory
University of Iowa College of Dentistry
Phone (319) 384 4433

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Re: [Histonet] Microtome

[Tony Auge]

Of the three microtomes that you listed I would highly recommend the Thermo
Shandon Finesse. It's of superior quality than the Microms I have used.
Cutting is excellent and the fly wheel action is light yet fluid. You do
have to oil it manually once a month but it's easy and you can clean it out
in the process and keep it in good operating condition. I haven't used the
Titan 5000 but I have used slides from Tanner that were of poor quality and
I ended up returning them.

I'm cutting on a used Leica 2125 right now. It is functional but it does
skip when facing and block orientation is terrible. Getting used lab
equipment is always a gamble so make sure your supplier is pleasant to deal
with and offers a warranty. Not sure what your budget is but a new Finesse
isn't too expensive for a new microtome and I'm sure you will be very happy
with it.


Tony Auge HTL (ASCP) QIHC
Histology Supervisor - Chandler Pathology Services
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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[Histonet] slide drying/curing before filing

[Lake, Kim S]

According to the Histonet archives, this hasn't been discussed for almost a 
decade.  How are labs drying/curing their slides before they are filed?

We are a small oral pathology laboratory and we hand-coverslip using Richard 
Allen mounting medium and glass coverslips.  After cases are signed out slides 
are placed in their cardboard folders in a 37C oven for at least a week, then 
are filed in metal slides drawers.

This works perfectly well, but we would like to simplify this, if possible.  A 
search of the archive showed a great deal of variation in slide drying 
protocol, I'm interested in hearing what everyone else is up to.  Of course, 
I'm hoping to get lots of people saying that they file all of last week's room 
temp slides on Monday morning and it works like a charm!

Thanks!

Kim Lake, MLS (ASCP)CM
Laboratory Manager
Oral Pathology Laboratory
University of Iowa College of Dentistry
Phone (319) 384 4433

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[Histonet] RE:Change in Her2 Neu Fixation

[Terri Braud]

As of the 4/2014 revision, CAP extended the fixation time to 72 hours.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874
Today's Topics:
  10. HER2 by IHC- Fixation  (Adesupo, Adesuyi (Banjo))
  11. RE: HER2 by IHC- Fixation (Weems, Joyce K.)

Message: 10
Date: Wed, 4 Jun 2014 11:20:32 -0500
From: "Adesupo, Adesuyi (Banjo)" 
Subject: [Histonet] HER2 by IHC- Fixation
Hi Histonetters,
 I hope you guys are doing great. Please I wanted to
confirm whether it is true that the CAP has changed the HER2 Fixation
time from 6 - 48 hours to 6 - 72 hours
  Thanks,
  Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145

That is true.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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[Histonet] RE: leaving histology---research still an option

[Teri Johnson]

Thank you Ray for your perspective. I wholeheartedly agree with you. A few 
(maybe more than a few) years back I wrote an article for NSH on transitioning 
from Clinical to Research. You'll find it here: 
http://www.nsh.org/content/transitioning-clinical-research

In most cases speed is not the goal. Instead a combination of efficiency, 
consistency and excellence is expected.

If what I have written doesn't appeal to you, then please keep your other 
options open.

Best wishes,

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752



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Re: [Histonet] Acid Cleaned glassware

[Rene J Buesa]

Venting the MW oven is just a safety issue and has nothing to do with the 
cleaning of the glassware and the quality of the staining procedure.
René J.  


On Wednesday, June 4, 2014 1:01 PM, "Abbott, Tanya" 
 wrote:
  


We just acquired a brand new laboratory microwave to replace ours from the 
1980s,..so we are optimizing our GMS stain. Currently we would let the 
glassware sit in
Chromic acid for 1 hr to chemically clean the glassware before staining. Now we 
are using the CAP required polypropylene vented microwave containers. Can we 
skip the chromic
Acid step?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] Acid Cleaned glassware

[Abbott, Tanya]

We just acquired a brand new laboratory microwave to replace ours from the 
1980s,..so we are optimizing our GMS stain. Currently we would let the 
glassware sit in
Chromic acid for 1 hr to chemically clean the glassware before staining. Now we 
are using the CAP required polypropylene vented microwave containers. Can we 
skip the chromic
Acid step?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] leaving histology question research is still an option

[koellingr]

Alpha Histotech, 
  
wanted to be sure that I did NOT tell you to drop everything in life to look to 
research exclusively.  So I cut and pasted this from my original message 
"Research histology should not be overlooked 
I stand by that statement. 
  
I agree with Emily that funding in research is (stupidly for this nation) 
difficult.  But it is not zero.  PhD's do NOT saturate histotech jobs in 
research labs.  There might be a few, maybe some, maybe a lot in some places 
but not all.  No one, even a clinical lab, will guarantee that job for years 
and years.  I know many histotech/non-histotech "techs" who have been through 
5-6 different more molecular labs in the same building for over 30 years.  So 
have 30 years seniority in the system.  Grant runs out and you move to a 
different lab (and is way easier having had made connections in first lab).  I 
made 3x in research industry then I could ever have made in clinical histo lab. 
 Is not for everyone but also not something to dismiss as hopeless.  If you 
ever do research, you will find that "N of 1" is not reliable to base 
everything and every decision on.  Best of luck.  Search for those options 
several others have given but don't dismiss my suggestion outright. 
  
Ray in Seattle (histotech from 1960's who could only retire now because of 
Research histology) 
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[Histonet] HER2 by IHC- Fixation

[Adesupo, Adesuyi (Banjo)]

 Hi Histonetters,
 I hope you guys are doing great. Please I wanted to 
confirm whether it is true that the CAP has changed the HER2 Fixation time from 
6 - 48 hours to 6 - 72 hours.


  Thanks,

  Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145

==
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RE: [Histonet] HER2 by IHC- Fixation

[Weems, Joyce K.]

That is true.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adesupo, 
Adesuyi (Banjo)
Sent: Wednesday, June 04, 2014 12:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HER2 by IHC- Fixation


 Hi Histonetters,
 I hope you guys are doing great. Please I wanted to 
confirm whether it is true that the CAP has changed the HER2 Fixation time from 
6 - 48 hours to 6 - 72 hours.


  Thanks,

  Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145

==
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[Histonet] RE: fluorescent scope and slide scanner

[Elizabeth Chlipala]

Albert

It all depends on your through put and how many slides you need to scan daily.  
There are so many other things you need to consider prior to purchasing a 
scanner.

If you go to the DPA website you can get a lot on information on digital 
pathology www.digitalpathologyassociation.org

The larger vendors such as Leica/Aperio, Olympus, Philips, GE, Ventana will 
have different solutions and packages for what you may need to include storage 
and database, reporting, image analysis, etc.  You need to think about how you 
want to use the scanner prior to purchasing one.  I have contact information 
for most of the vendors so if you want me to provide that to you I can.

Now I'm going to give a shameless plug - Jesus Elin, Bill DeSalvo and myself 
will be giving an all day workshop on this very topic at NSH in Austin it's on 
Saturday.  If you can't make it to that meeting then the DPA has Pathology 
Visions in October you would be able to spend time with a lot of the vendors 
that are in this digital pathology space and in addition to that Bill DeSalvo 
and I will also presenting on this topic at the Region VII meeting in Phoenix 
in July.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Santiago, Albert
Sent: Wednesday, June 04, 2014 6:42 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] fluorescent scope and slide scanner

Hello my colleagues in histoland,
We are in the market for a  Slide Scanner and a Fluorescent Microscope, if 
anyone has any information on any of these products please share with me.
Thank you


Albert Santiago, HT(ASCP)
Lab Manager
Penncutaneous Pathology Services
Dermatopathology Lab
3020 Market ST. Ste 201
Philadelphia, PA 19104
215-662-6539 - Lab
215-662-6759-office
albert.santi...@uphs.upenn.edu

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Re: [Histonet] Should I leave histology world

[Emily Brown]

As someone who has been in research (basically being a histologist), I can
say that there are NO jobs out there for you.  The market is saturated with
PhDs.  Do not leave your job for a research position unless they can
guarantee your salary for years.  This will be very unlikely, as getting a
grant is super hard nowadays.
I actually have to be ceritifed to work in a clinical lab, but I know that
after 15 years in a lab, I definitely have the skills, just not the
certification to be in a clinical lab.  I am working in the office now, and
in the lab one day a week after having an R01 for ten years and being the
lab manager in a research lab.  I'm going to get certification in case this
office/lab thing doesn't work out in a few years.  I wish there was more
money in science but there isn't.
So the main point is, either get some skills, or go a different path.
 Research is not where it's at right now.
Although, I am assuming you're in the US, this might not be the case in
other countries.

Emily


"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"


On Wed, Jun 4, 2014 at 7:45 AM, joelle weaver 
wrote:

> Yes thanks for the perspective. I have a bias towards my own experience,
> and this seems to be good advice. I work in a molecular based lab now and
> they are very unaware of what it typically is like in a clinical
> histopathology lab. Good to point other environments are out there that are
> clinical, and also that research in general can be very different than
> clinical settings. Some people are just more suited to certain environments
> over the other.
>
>
>
>
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>
> Date: Wed, 4 Jun 2014 01:32:11 +
> From: koelli...@comcast.net
> To: joellewea...@hotmail.com
> CC: timothy.mor...@ucsfmedctr.org; optimusprimehistot...@hotmail.com;
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Should I leave histology world
>
> Alpha Histotech-
>
> I'll put in my few words even though I'm not active anymore and possibly
> from different perspective.  But also using a few assumptions and if my
> assumptions are wrong then the rest of what I say is probably meaningless.
>  Not IDíng your e-mail address but if you've worked 3 jobs nightshift
> including a large reference lab, do you live near a big city?  And if so is
> it a city close to a college or university.
>
> Research histology should not be overlooked.  You will find many molecular
> or other such non-histo labs that actually do some or even a lot of
> histology by non-histology personnel or lab workers.  Sometimes it is OK,
> sometimes even great.  Sometimes, and I witnessed it, it is at an
> embarrassing histo level.  I can walk up or down university hallways and
> see a "genetics lab" or some other "molecular lab" and see a microtome or
> cryostat in there.  Sometimes those PI's will send histo work to a core
> lab.  Sometimes they don't want to pay per block so do it (and staining and
> IHC and FISH) themselves.  Someone with even minimal wide-ranging histo
> experience might be welcomed.
>
> No timed block cutting counts.  Learn some immunology, genetics, molecular
> techniques, comparative medicine, physiology, etc, etc along the way.  Many
> places even pay for college level courses while employed there.
>
> Just a thought if you are near that kind of area.
>
> Ray in Seattle
>
>
>
>
>
> From: "joelle weaver" 
> To: "Timothy Morken" , "Alpha Histotech" <
> optimusprimehistot...@hotmail.com>, histonet@lists.utsouthwestern.edu
> Sent: Tuesday, June 3, 2014 5:55:09 PM
> Subject: RE: [Histonet] Should I leave histology world
>
>
> It would be a shame to get discouraged now after all the time you have
> already put into histology. If you still want to work in histology, I might
> suggest you try to have a conversation with a manager, supervisor or lead
> tech and see if they are willing to support you. Tell them you want to
> spend more time cuting to be able to section with high quality at the rate
> that works for their productivity standards.  If you present it as a
> win-win proposition, see what resources, people and time they are willing
> to "chip in"  to help get where they would like you to be. Make some metric
> or rate to achieve in microtomy your goal for the year, and put it into
> writing ( good for all goals:).
> Or if that is too uncomfortable , approach someone individually whose
> microtomy skills you admire , and see if they are willing to provide some
> tips and guidance off work time.
>
> I also went through a NAACLS program.  Still at my first "real" histology
> job , the realization that this was the actual training became apparent
> very quickly.  I had moments of exhaustion and feeling overwhelmed, but I
> now feel I was also fortunate to work initially at a pretty high volume
> place. It was a great "breaking in" for embeddin

RE: [Histonet] Should I leave histology world

[Morken, Timothy]

Rene is right that everyone needs to find what they are good at. We had a guy 
who was so-so in cutting speed and always getting flack for not doing enough. 
And had such a hard time coordinating specials and immunos that he just slowed 
things down.  Then we started doing Mega blocks of whole mount eyes and 
prostate and it turns out this guy is a savant at cutting large blocks. The 
pathologists were raving about how good the sections were. He  had a job as 
long as he wanted  in that lab just for that!



Tim Morken


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, June 04, 2014 7:41 AM
To: Alpha Histotech; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Should I leave histology world

You describe a common situation in histology where the pressure to finish the 
work on time (the TAT) reigns but that does not necessarily mean that quality 
has to be sacrificed, although sometimes it does.
On the other hand, not all histotechs are "created equal" and some have 
abilities others don't. Manual dexterity is a most and while some can section 
at the "normal" rate of 24 blocks/hour, other take twice the time, and it seems 
that you belong to the later group.
For what you wrote it seems that your embedding productivity (about 60 
blocks/hour) is the "norm" and, again, others are less or more productive. You 
also point out that you have worked in 3 places in 6-7 years and that is a 
quite high turnover never conducent to improve your work and that can be hold 
"against you" in your Resumé when looking for another position.
A histology supervisor has to assure the slides are ready for the pathologists 
on a timely basis so one of the things that have to be done is to identify 
amongst the staff  "who does what best" meaning who can deliver quality in a 
timely basis.
I think that you, already in your late 20's, should start trying to answer 
several questions:
1- why did you in the first place decided to become a histotech and if that was 
a "wise" selection?
2- if you are experiencing the same problems in the 3 places where you worked, 
it seems clear to me that the issue is with you and not with the trade. It will 
be the same at least in these types of high volume labs where higher 
productivity are required.
If you really like histology and think that you need more training to achieve 
sectioning speed, you have to switch to another type of lab. Try to solicit 
work in a research or university lab where you will have enough time to train 
properly and where finishing the work "yesterday" is no usually a concern.
Also think that not all histotechs have the same ability or speed. I had 
supervised along my career as supervisor scores of histotechs and some just 
cannot section fast, it is not within their abilities but can excel in some 
other tasks. My duty was to detect the task that they could complete best and, 
without totally frustrating their lives, assign them to those tasks.
Also you could try to improve your speed on your own time, and demonstrate that 
you are also willing to try to improve.
So I think that it is time for you to do one of the following:
1- change career
2- change type of lab
3- adapt and adquire sectioning speed or
4- find amongst the many tasks that histology provide, the one where you can 
excel and at the same find satisfaction.
What you cannot do is to keep doing the same and expect to find satisfaction or 
obtain a different result given your ability.
Think hard and honestly what you want to do for the rest of your life that most 
likely will be long.
René J.  


On Tuesday, June 3, 2014 4:35 PM, Alpha Histotech 
 wrote:
  


Hi everyone,

I wouldn't give too much detail information as the histology world is very 
small and everyone knows everyone.

I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went 
to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs 
I have changed jobs 3 times. All the jobs were graveyard shifts. The first 
place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 
places I won't mention and I currently still have a histology job. My problem 
is all the places I worked were factory style lab work and they all did derm 
work. In my career I really only embedded most of the time. I did occasional 
other stuff like special stains both by hand and using Dako Artisan and other 
things like cytology cytospin. But I never got to develop in cutting. My first 
job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me 
and put me back to embed. My 2nd job put me to cut the last 2 months (full 
8hrs) I was working there. My current job I have been cutting since April 2014 
( but only 2-3hrs in the day and  then I embed, I have been here now 1 yr, I 
was embedding most of the time before th cutting started). I was told by my 
director I need to 

[Histonet] Pathologists?

[Gudrun Lang]

Dear histonetters!

Maybe you can help me.  We are looking for pathologists for our clinical
histolab in Linz, Austria. Anyone (German speaking), who is interested in is
welcome to inform him- or herself on this website.

http://www.linz.at/akh/10374.asp

 

We have a well equipted, modern histolab (from routine histo till FISH and
molecularpathology). 

Best regards

Gudrun 

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[Histonet] Clearium?

[Abbott, Tanya]

This is a bit more of a Cytology question, but I thought I would survey my 
Histo friends! We are starting to have bubbles with our PAP slides, and it 
actually seems to occur after drying, initially after coverslipping (by hand) 
there appears to be no bubbles. We use Clearium, so we can go directly from 
alcohol. I am a new manager, and this lab is very adversed to Xylene.  Any 
ideas?
Thanks!

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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Re: [Histonet] fluorescent scope and slide scanner

[Rene J Buesa]

Contact a Leica Microsystems representative.
René J. 


On Wednesday, June 4, 2014 8:42 AM, "Santiago, Albert" 
 wrote:
  


Hello my colleagues in histoland,
We are in the market for a  Slide Scanner and a Fluorescent Microscope, if 
anyone has any information on any of these products please share with me.
Thank you


Albert Santiago, HT(ASCP)
Lab Manager
Penncutaneous Pathology Services
Dermatopathology Lab
3020 Market ST. Ste 201
Philadelphia, PA 19104
215-662-6539 - Lab
215-662-6759-office
albert.santi...@uphs.upenn.edu

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[Histonet] HTL program

[Bilger, Andrea]

I have several techs who used Indiana University's on line course and they are 
all great techs!  Have not been impressed with techs from Harford Community's 
program.

Andrea Bilger, HTL
Team Leader, Histology
York Hospital
1001 S. George St.
York, Pa.  17405
(717) 851-5040


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[Histonet] RE: Histonet Digest, Vol 127, Issue 3

[Nancy Schmitt]

Hi Denise-
I would HIGHLY recommend the online program at IUPUI - we have had 4 people go 
through this program including myself.
When finished you are ready to sit for the test! It is really well done and 
they are so helpful along the way.
Contact info: Debra M. Wood, Director,
 Histotechnology Program
 Clinical Assistant Professor
 Indiana University School of Medicine
 Department of Pathology & Laboratory Medicine
 350 W. 11th St. ROOM 6002A
 Indianapolis, IN 46202
 Program Office Phone: 317-491-6410

Good Luck!
Nancy Schmitt MLT, HT(ASCP)
United Clinical Laboratories
Dubuque, IA



_
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smith, Denise
Sent: Tuesday, June 03, 2014 12:18 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] HTL Online Courses?

Hi all!

I have been doing massive research on this for past several days but no solid 
leads.  I am wondering about HT/HTL Histology online courses... I live in St. 
Louis Missouri and they don't offer any histology courses around here - only 
out of states.  I cannot relocate or do 1-2 years program out of state due to 
my full-time job and family reason.

Do anyone know any good Histology online courses that I can take without 
conflicting with my job?

Greatly appericated!  Thanks!

Denise Smith

smit...@kids.wustl.edu

Re: [Histonet] Should I leave histology world

[Rene J Buesa]

You describe a common situation in histology where the pressure to finish the 
work on time (the TAT) reigns but that does not necessarily mean that quality 
has to be sacrificed, although sometimes it does.
On the other hand, not all histotechs are "created equal" and some have 
abilities others don't. Manual dexterity is a most and while some can section 
at the "normal" rate of 24 blocks/hour, other take twice the time, and it seems 
that you belong to the later group.
For what you wrote it seems that your embedding productivity (about 60 
blocks/hour) is the "norm" and, again, others are less or more productive. You 
also point out that you have worked in 3 places in 6-7 years and that is a 
quite high turnover never conducent to improve your work and that can be hold 
"against you" in your Resumé when looking for another position.
A histology supervisor has to assure the slides are ready for the pathologists 
on a timely basis so one of the things that have to be done is to identify 
amongst the staff  "who does what best" meaning who can deliver quality in a 
timely basis.
I think that you, already in your late 20's, should start trying to answer 
several questions:
1- why did you in the first place decided to become a histotech and if that was 
a "wise" selection?
2- if you are experiencing the same problems in the 3 places where you worked, 
it seems clear to me that the issue is with you and not with the trade. It will 
be the same at least in these types of high volume labs where higher 
productivity are required.
If you really like histology and think that you need more training to achieve 
sectioning speed, you have to switch to another type of lab. Try to solicit 
work in a research or university lab where you will have enough time to train 
properly and where finishing the work "yesterday" is no usually a concern.
Also think that not all histotechs have the same ability or speed. I had 
supervised along my career as supervisor scores of histotechs and some just 
cannot section fast, it is not within their abilities but can excel in some 
other tasks. My duty was to detect the task that they could complete best and, 
without totally frustrating their lives, assign them to those tasks.
Also you could try to improve your speed on your own time, and demonstrate that 
you are also willing to try to improve.
So I think that it is time for you to do one of the following:
1- change career
2- change type of lab
3- adapt and adquire sectioning speed or
4- find amongst the many tasks that histology provide, the one where you can 
excel and at the same find satisfaction.
What you cannot do is to keep doing the same and expect to find satisfaction or 
obtain a different result given your ability.
Think hard and honestly what you want to do for the rest of your life that most 
likely will be long.
René J.  


On Tuesday, June 3, 2014 4:35 PM, Alpha Histotech 
 wrote:
  


Hi everyone,

I wouldn't give too much detail information as the histology world is very 
small and everyone knows everyone.

I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I went 
to a NAACLS school and have a Associate in Science in Histology. In the 6-7 yrs 
I have changed jobs 3 times. All the jobs were graveyard shifts. The first 
place I worked for was Quest Diagnostics and I did a good 3 yrs. The other 2 
places I won't mention and I currently still have a histology job. My problem 
is all the places I worked were factory style lab work and they all did derm 
work. In my career I really only embedded most of the time. I did occasional 
other stuff like special stains both by hand and using Dako Artisan and other 
things like cytology cytospin. But I never got to develop in cutting. My first 
job in quest..I maybe cutted one time for 2 or 3 weeks before they yanked me 
and put me back to embed. My 2nd job put me to cut the last 2 months (full 
8hrs) I was working there. My current job I have been cutting since April 2014 
( but only 2-3hrs in the day and
 then I embed, I have been here now 1 yr, I was embedding most of the time 
before th cutting started). I was told by my director I need to speed up in 
cutting because corporate is asking why I am not increasing in speed. And if I 
don't speed up eventually then they will have to demote me to a lab aid and 
give me a pay cut. (where I work and the state I work in they have lab aids 
doing alot of stuff without being certified, it wasn't like that in the other 
state I am original from as you have to be state licensed and ascp) I sometimes 
laugh inside my head because before my director hired me I told him I don't 
have alot experience in cutting. 

Now everywhere I have gone...speed is the name of the game. They say they care 
about quality but in the end if you can't put up then you will be put out!  So 
I am just thinking I should just get out of histology world all together. Every 
where I have worked unfortunately have management who believe quantity 

[Histonet] Practice tissue?

[Nutter, James E]

Go to the local pet store and get a feeder rat (can buy frozen ones).
Gross it and process.  Great for practice tissues, can get the full
range from brain to kidney.

 

Good luck!

 

James E. Nutter Jr. BS, HT & QIHC(ASCP)

Quest Diagnostics Nichols Institute | Histology| 14225 Newbrook Dr.|
Chantilly Va. USA | phone 703.802.6900 x65782| fax 703.802.7191| |
james.e.nut...@questdiagnostics.com
  | www.NicholsInstitute.com
 

 


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[Histonet] fluorescent scope and slide scanner

[Santiago, Albert]

Hello my colleagues in histoland,
We are in the market for a  Slide Scanner and a Fluorescent Microscope, if 
anyone has any information on any of these products please share with me.
Thank you


Albert Santiago, HT(ASCP)
Lab Manager
Penncutaneous Pathology Services
Dermatopathology Lab
3020 Market ST. Ste 201
Philadelphia, PA 19104
215-662-6539 - Lab
215-662-6759-office
albert.santi...@uphs.upenn.edu

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RE: [Histonet] Should I leave histology world

[joelle weaver]

Yes thanks for the perspective. I have a bias towards my own experience, and 
this seems to be good advice. I work in a molecular based lab now and they are 
very unaware of what it typically is like in a clinical histopathology lab. 
Good to point other environments are out there that are clinical, and also that 
research in general can be very different than clinical settings. Some people 
are just more suited to certain environments over the other.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
Date: Wed, 4 Jun 2014 01:32:11 +
From: koelli...@comcast.net
To: joellewea...@hotmail.com
CC: timothy.mor...@ucsfmedctr.org; optimusprimehistot...@hotmail.com; 
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Should I leave histology world

Alpha Histotech-

I'll put in my few words even though I'm not active anymore and possibly from 
different perspective.  But also using a few assumptions and if my assumptions 
are wrong then the rest of what I say is probably meaningless.  Not IDíng your 
e-mail address but if you've worked 3 jobs nightshift including a large 
reference lab, do you live near a big city?  And if so is it a city close to a 
college or university.

Research histology should not be overlooked.  You will find many molecular or 
other such non-histo labs that actually do some or even a lot of histology by 
non-histology personnel or lab workers.  Sometimes it is OK, sometimes even 
great.  Sometimes, and I witnessed it, it is at an embarrassing histo level.  I 
can walk up or down university hallways and see a "genetics lab" or some other 
"molecular lab" and see a microtome or cryostat in there.  Sometimes those PI's 
will send histo work to a core lab.  Sometimes they don't want to pay per block 
so do it (and staining and IHC and FISH) themselves.  Someone with even minimal 
wide-ranging histo experience might be welcomed.

No timed block cutting counts.  Learn some immunology, genetics, molecular 
techniques, comparative medicine, physiology, etc, etc along the way.  Many 
places even pay for college level courses while employed there.

Just a thought if you are near that kind of area.

Ray in Seattle





From: "joelle weaver" 
To: "Timothy Morken" , "Alpha Histotech" 
, histonet@lists.utsouthwestern.edu
Sent: Tuesday, June 3, 2014 5:55:09 PM
Subject: RE: [Histonet] Should I leave histology world


It would be a shame to get discouraged now after all the time you have already 
put into histology. If you still want to work in histology, I might suggest you 
try to have a conversation with a manager, supervisor or lead tech and see if 
they are willing to support you. Tell them you want to spend more time cuting 
to be able to section with high quality at the rate that works for their 
productivity standards.  If you present it as a win-win proposition, see what 
resources, people and time they are willing to "chip in"  to help get where 
they would like you to be. Make some metric or rate to achieve in microtomy 
your goal for the year, and put it into writing ( good for all goals:). 
Or if that is too uncomfortable , approach someone individually whose microtomy 
skills you admire , and see if they are willing to provide some tips and 
guidance off work time. 
 
I also went through a NAACLS program.  Still at my first "real" histology job , 
the realization that this was the actual training became apparent very quickly. 
 I had moments of exhaustion and feeling overwhelmed, but I now feel I was also 
fortunate to work initially at a pretty high volume place. It was a great 
"breaking in" for embedding and microtomy.   Luckily there were also some 
experienced techs there who saw how much I wanted to learn,  and were willing 
to help me get better. The "constructive" criticism stung sometimes, but they 
did me a huge service. But believe me,  not everyone was helpful or supportive 
along the way. Try to ignore those kind of people as much as possible. And I 
still get criticized sometimes, make mistakes, and I still have more to learn.
 
But here are a couple of other options for you to consider before you decide to 
leave, and what  I did to get more experience  when in your situation more 
quickly; 
 
Take on a second histology job that targets specific skills, tissue, or 
techniques you want more experience in. Believe me I have been criticized and 
misunderstood for this choice s well many times, but personally I do not regret 
any of those experiences now.
 
I also feel that small labs are nice to build well rounded skills since you are 
usually more of a "jack of all trades" and have less tendency to do one task 
over your whole shift from day to day. Sometimes you just have to identify the 
environment that is the right fit for you. 
 
Best of luck to you- and let us know how things turn out!








Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: timothy.mor...@ucsfmedctr.org
> To: optimusprimehistot...@hotmail.com; histonet@lists.utsouthwestern.edu
> Date: Tue, 3 Jun 2014 22:5

Re: [Histonet] Microtome

[Ronda Mire]

Agree, Leica is the best hands down.

On Jun 3, 2014, at 5:37 PM, nmhi...@comcast.net wrote:

> If someone promised me a brand new FREE microtome and it wasn't a LEICA, I'd 
> tell them, "no, thanks!".  I am not inclined to endorse any other brand and I 
> cannot imagine changing my mind about that. 
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> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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