Re: [Histonet] Should I leave histology world

[Lee & Peggy Wenk]

You didn't mention what your current cutting speed is, nor what speed they 
want you to be at.


Ask your supervisor to set up goals for you to meet, with time frames. Each 
week, work on getting faster, not fast today, just faster each week.


At the School where I taught, we had goals set up during the time the 
students were learning, and also time when they were doing their histology 
lab rotation. When they were learning in the School, they would microtome 
1-3 times a week for the first 2 months, and nearly daily the last 2 months, 
about 2 hours a day (for total of 120+ hours microtoming before their 
histology rotation). The first couple of months, emphasis was on quality, 
and then it shifted to quantity (speed) and quality. Every 4-6 weeks, they 
were reassessed as to whether they were meeting quality and quantity (speed) 
goals.


When they did their 1 month rotation through histology lab, they were full 
time in the lab, and microtomed about 2-3 hours a day (total about an 
additional 100 hours microtoming). They were expected to keep the quality 
up, and still improve their quantity (speed). When they graduated, no, they 
were not as fast as experienced techs, but they were close.


At any time (school or rotations), if they didn't measure up to the goals, 
they knew they had to repeat the evaluation and/or rotation. If they still 
could not meet the goals, they would be let go from the program. So they had 
to pass the academic part (tests for what they know) as well as psychomotor 
(DOING the sectioning, staining, embedding, etc.), and the affective 
(showing up on time, getting along with people, willing to volunteer, etc.).


This is the same as any histotech job. There are goals (quality and quantity 
(speed)) that everyone must be able to meet. You have been microtoming at 
your second job for 2 months, 8 hours a day, and 2-3 hours a day since April 
(up to 2 months) at your current job. That's about 400 hours of microtoming 
while at jobs. At this point, you should have quality down pat, and should 
be able to concentrate on speed.


So have your supervisor set specific speed goals and dates, and then you use 
those time intervals to keep improving your speed. Have your supervisor 
watch you, and see where you are being slowed down by unnecessary movements. 
And if you are the only histotech responding to the data entry person, then 
explain it to your supervisor, and get that responsibility on a rotating 
basis amongst all of you.


Peggy Wenk HTL (ASCP) SLS


-Original Message- 
From: Alpha Histotech

Sent: Tuesday, June 03, 2014 4:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Should I leave histology world

Hi everyone,

I wouldn't give too much detail information as the histology world is very 
small and everyone knows everyone.


I am in a dilemma. I have been a histotech (ASCP HT) for almost 6-7 yrs. I 
went to a NAACLS school and have a Associate in Science in Histology. In the 
6-7 yrs I have changed jobs 3 times. All the jobs were graveyard shifts. The 
first place I worked for was Quest Diagnostics and I did a good 3 yrs. The 
other 2 places I won't mention and I currently still have a histology job. 
My problem is all the places I worked were factory style lab work and they 
all did derm work. In my career I really only embedded most of the time. I 
did occasional other stuff like special stains both by hand and using Dako 
Artisan and other things like cytology cytospin. But I never got to develop 
in cutting. My first job in quest..I maybe cutted one time for 2 or 3 weeks 
before they yanked me and put me back to embed. My 2nd job put me to cut the 
last 2 months (full 8hrs) I was working there. My current job I have been 
cutting since April 2014 ( but only 2-3hrs in the day and then I embed, I 
have been here now 1 yr, I was embedding most of the time before th cutting 
started). I was told by my director I need to speed up in cutting because 
corporate is asking why I am not increasing in speed. And if I don't speed 
up eventually then they will have to demote me to a lab aid and give me a 
pay cut. (where I work and the state I work in they have lab aids doing alot 
of stuff without being certified, it wasn't like that in the other state I 
am original from as you have to be state licensed and ascp) I sometimes 
laugh inside my head because before my director hired me I told him I don't 
have alot experience in cutting.


Now everywhere I have gone...speed is the name of the game. They say they 
care about quality but in the end if you can't put up then you will be put 
out!  So I am just thinking I should just get out of histology world all 
together. Every where I have worked unfortunately have management who 
believe quantity over quality. OR Do you guys think I need more time cutting 
to develop speed? Beforehand I did need a little learning curve to cut and I 
have gotten through that now. It's just the speed that is killing me. And I 
als

[Histonet] RE: Churukian Silver stain for Fungus

[Tony Henwood (SCHN)]

Hi Terri,

I agree, Churukian's Ammoniacal silver method is very good.

I assume you are referring to the method given in Churukian, C. J., & Schenk, 
E. A. (1988). Staining Pneumocystis carinii and fungi in unfixed specimens with 
ammoniacal silver using a microwave oven. Journal of Histotechnology, 11(1), 
19-21.

In their discussion they note:
"Paraffin sections can also be stained with this method with only minor 
changes. In place of 5% periodic acid use 5% chromic acid"
Though the reason given is that paraffin sections tend to detach from slides.

Charles repeats this recommendation in his later article: Churukian, C. J. 
(1996). Consistent silver methods for demonstrating basement membranes, 
reticulum, and fungi. Journal of histotechnology, 19(3), 211-217.

I follow Charles recommendations for paraffin sections (ie chromic acid).

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: Terri Braud [mailto:tbr...@holyredeemer.com] 
Sent: Friday, 6 June 2014 10:50 PM
To: Tony Henwood (SCHN)
Subject: RE: Churukian Silver stain for Fungus

Tony -
I totally agree with you (and your references) that Periodic Acid is not as 
good when used with Grocott's methenamine silver as in the GMS.
However, it totally rocks when used with the Churukian Ammoniacal Silver 
Method.  We are still talking about 2 different staining methods.
Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

-Original Message-
From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au]
Sent: Thursday, June 05, 2014 9:17 PM
To: Terri Braud; histonet@lists.utsouthwestern.edu
Subject: RE: Churukian Silver stain for Fungus

Terri,

I was referring to the incorrect use of periodic acid in the GMS stain.

The Grocott's Methenamine silver stain uses chromic acid, not Periodic acid.
There are many instances in the literature where using a classic GMS has been 
found superior for demonstrating fungus rather than PAS. 
 I am concerned that Periodic acid - silver staining has not been validated 
using cases where classic GMS has been shown to be more sensitive than PAS (see 
below) or in cases of pseudofungi, which are PAS positive but overall GMS 
negative.

An appreciation of the difficulties in relying on Periodic acid rather than 
chromic acid for GMS staining can be seen in these articles: 
Chandler, F. W., & Watts, J. C. (1995). Fungal diseases. Journal of 
Histotechnology, 18(3), 247-252

Carson, F. L., Fredenburgh, J., & Maxwell, J. E. (1999). Inconsistent detection 
of histoplasma capsulatum with periodic acid oxidation in the Grocott 
methenamine-silver nitrate (GMS) fungus stain. Journal of histotechnology, 
22(2), 119-122.

Carson, F. L., & Richmond, R. S. (2010). Periodic acid cannot replace chromic 
acid in Grocott's method for fungi. Biotechnic & histochemistry:
official publication of the Biological Stain Commission, 85(4), 270.

Henwood, A. F., Prasad, L., & Bourke, V. M. (2013). The application of heated 
detergent dewaxing and rehydration to techniques for the demonstration of 
fungi: a comparison to routine xylene-alcohol dewaxing.
Journal of Histotechnology, 36(2), 45-50.

Song, D. E., Kahn, A. G., Khang, S. K., & Ro, J. Y. (2005). Pseudofungi in 
pericolic lymph nodes. Archives of pathology & laboratory medicine, 129(4), 
e97-e100.



Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory 
Manager & Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, 
School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead 
NSW 2145, AUSTRALIA 

-Original Message-
From: Terri Braud [mailto:tbr...@holyredeemer.com]
Sent: Thursday, 5 June 2014 11:29 PM
To: Tony Henwood (SCHN); histonet@lists.utsouthwestern.edu
Subject: RE: Churukian Silver stain for Fungus

I beg to differ.  I think you are confusing stains. I am not talking about 
using a Periodic Acid Schiff's stain (PAS) to stain fungus.  I am referring to 
the Churukian Microwave Ammoniacal Silver stain for fungus.
It oxidizes with Periodic Acid and the Silver solution is similar to what is 
used for most Reticulum stains. It stains the exact same organisms as a 
Grocott's Methenamine Silver (GMS), just without staining the elastic fibers. 
Our pneumocystis stains using the Churukian Ammoniacal Silver stain are just 
beautiful.
This method has been tau

[Histonet] RE: Churukian Silver stain for pneumocystis and fungus

[Tony Henwood (SCHN)]

Hi Terri,

I agree, Churukian's Ammoniacal silver method is very good.

I assume you are referring to the method given in Churukian, C. J., & Schenk, 
E. A. (1988). Staining Pneumocystis carinii and fungi in unfixed specimens with 
ammoniacal silver using a microwave oven. Journal of Histotechnology, 11(1), 
19-21.

In their discussion they note:
"Paraffin sections can also be stained with this method with only minor 
changes. In place of 5% periodic acid use 5% chromic acid"
Though the reason given is that paraffin sections tend to detach from slides.

Charles repeats this recommendation in his later article: Churukian, C. J. 
(1996). Consistent silver methods for demonstrating basement membranes, 
reticulum, and fungi. Journal of histotechnology, 19(3), 211-217.

I follow Charles recommendations for paraffin sections (ie chromic acid).


Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud
Sent: Friday, 6 June 2014 11:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Churukian Silver stain for pneumocystis and fungus

For those who have written off list for the Churukian stain procedure, I've 
included it here.  It is also sold in the USA in kit form by Polyscientific (no 
plug here, just stating)
PRINCIPLE: 
Fungi and Pneumocystis organisms are oxidized using periodic acid and then 
impregnated with ammoniacal silver solution.  The silver is then toned from 
brown to black with gold chloride.  Any unreduced silver is removed with sodium 
thiosulfate, leaving organisms stained black against a green counterstained 
background.

SPECIMENS:
Formalin fixed, paraffin embedded tissue cut at 4-5 microns or 95% alcohol 
fixed smears or monolayer cell preparations.

CONTROL: 
Any tissue known to contain a fungus or Pneumocystis organisms

REAGENTS:
1.  Periodic acid 1% Aqueous
2.  Ammoniacal GMS solution (Silver)
3.  Gold Chloride 0.5% Aqueous
4.  Sodium Thiosulfate 5% Aqueous
5.  Fast Green Working Solution
All working solutions are purchased from PolyScientific Kit #K101.
Individual components may be purchased separately as they are exhausted.

PROCEDURE: 
1.  Deparaffinize and hydrate to water
2.  Using a plastic Coplin jar with the lid resting upside down on top, 
microwave 50.ml of 1% Periodic Acid at for 30 seconds at power 5.
3.  Place slides in the hot solution, covered, for 5 minutes.
4.  Wash in 5 changes of DI Water.
5.  Using a plastic Coplin jar with the lid resting upside down on top, 
microwave 40.ml of Ammoniacal Silver Solution at for 30 seconds on HIGH power.
6.  Using a plastic disposable pipet, vigorously stir the solution for a 3 
seconds.
7.  Place the slides in the hot silver solution
8.  Replace the lid upside down on top of the Coplin jar and microwave for 
an additional 20 seconds on power 5.
9.  For fungus, let the slides stand for exactly 1 minute following the 
last microwave step.  For pneumocystis, let the slides sit for exactly 2 
minutes following the last microwave step/
10. Wash in 5 changes of DI Water
11. With slides lying flat, dispense enough Gold Chloride to cover the 
slide for 1 minute.  Rinse in DI Water.
12. With slides lying flat, dispense enough Sodium Thiosulfate solution to 
cover the slide for 2 minutes.  Rinse in DI Water.
13. With slides lying flat, dispense enough Light Green to cover the slide 
for 1 minute.  Rinse in DI Water.
14. Dehydrate through graded alcohols, clear in 2 changes of Xylene and 
mount with a compatible mounting media.

NOTE:  Microwaves differ in power output.  This procedure is based on our 
current microwave.  If the power changes or the microwave is changed, then the 
timing of this procedure may have to be adjusted and the stain revalidated.
 
RESULTS
Fungi – Black
Pneumocystis – Black to blue black
Mucin – Pale Gray
Background - Green

REFERENCE:

Kit Procedure Insert, Rapid GMS for Fungus and Pneumocystis Carinii, Cat# k101, 
Poly Scientific R&D Corporation, Bay Shore NY 11706

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874


**

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Re: [Histonet] Leica special/h&e stainer

[Michael Backhus]

On GI we always did as follows:

Duodenum H&E
Antrum HP/AB
Greater/Lesser Curvature HP/AB
Esophagus AB/PAS
GE Junction HP/AB/PAS

If there were multiply esophagus sites at different centimeters we did specials 
on everyone. 

The HP was not a IHC

I was always curious if this was too many stains.
Mike


Sent from my iPhone

> On Jun 9, 2014, at 6:23 PM, "Sanders, Jeanine (CDC/OID/NCEZID)" 
>  wrote:
> 
> We have one as well but the special stains function for us was just something 
> we thought we might use...we did purchase the bucket inserts just in case. 
> But it was incompatible with simultaneously ding H&E's so we bought a Dako 
> Artisan just for specials and love it.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
> Sent: Monday, June 09, 2014 3:07 PM
> To: Deloris Carter; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Leica special/h&e stainer
> 
> I have the Leica Multistainer.  It was purchased before I got here.  The only 
> thing I don't like is that the programs have to be "compatible" or else the 
> machine alarms when you load a special stain onto the machine when it already 
> has an H&E going.  There's a rather complicated way to get the programs to be 
> compatible which was explained to me when I went for training.  You have to 
> use an excel spreadsheet and figure out what rinse is where during all the 
> programs.  Yes, it's something Leica's engineers have to work on.
> 
> Barbara S. Tibbs
> Histology Supervisor
> Accurate Diagnostic Labs
> South Plainfield, NJ
> barbara.ti...@accuratediagnosticlabs.com
> 732-839-3374
> Cell: 610-809-6508
> 
> 
> 
> From: histonet-boun...@lists.utsouthwestern.edu 
>  on behalf of Deloris Carter 
> 
> Sent: Monday, June 09, 2014 5:48 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Leica special/h&e stainer
> 
> Does anyone have any feedback on the Leica Multistainer? It stains H&E as 
> well as special stains.
> 
> Thanks,
> Deloris Carter, HT(ASCP)
> Shawnee Mission Medical Center
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RE: [Histonet] Leica special/h&e stainer

[Sanders, Jeanine (CDC/OID/NCEZID)]

We have one as well but the special stains function for us was just something 
we thought we might use...we did purchase the bucket inserts just in case. But 
it was incompatible with simultaneously ding H&E's so we bought a Dako Artisan 
just for specials and love it.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Monday, June 09, 2014 3:07 PM
To: Deloris Carter; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Leica special/h&e stainer

I have the Leica Multistainer.  It was purchased before I got here.  The only 
thing I don't like is that the programs have to be "compatible" or else the 
machine alarms when you load a special stain onto the machine when it already 
has an H&E going.  There's a rather complicated way to get the programs to be 
compatible which was explained to me when I went for training.  You have to use 
an excel spreadsheet and figure out what rinse is where during all the 
programs.  Yes, it's something Leica's engineers have to work on.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Deloris Carter 

Sent: Monday, June 09, 2014 5:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica special/h&e stainer

Does anyone have any feedback on the Leica Multistainer? It stains H&E as well 
as special stains.

Thanks,
Deloris Carter, HT(ASCP)
Shawnee Mission Medical Center
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[Histonet] Re: G.I. biopsies and special stains

[Bob Richmond]

Kathy Machado at Danville, Virginia, Regional Medical Center asks:

>>I was wondering if other labs are "automatically" ordering special stains
and/or IHC on stomach biopsies and esophageal biopsies? Or do you wait
until H&E is screened?<<

A number of fairly small labs I've worked in in recent years all do
whatever studies they do at all on all biopsies. What they do varies
greatly - and usually the pathologist isn't allowed to ask for anything not
done on every biopsy.

Specifically: Every lab I've seen does either a stain or IHC for
Helicobacter on all gastric biopsies. A few do Alcian blue on all
esophageal biopsies. I've never seen a lab that did any stain routinely on
duodenal biopsies, where the pathologist is deliriously happy just to get
consistently interpretable H & E slides.

I've been asking around about routine CD3 on duodenal biopsies. A fair
number of labs do it, but G.I. pathologists caution you not to, because
there are no published standards for interpreting this stain.

As you're probably aware, the regulatory agency for the Virginias and the
Carolinas is planning to put a stop to all routine G.I. biopsy special
stains, and limit Helicobacter staining to 20% of gastric biopsies.

I think this is acceptable practice, provided that there is a serious
quality assurance program for G.I. biopsy slides, something I've yet to see.

Bob Richmond
Samurai Pathologist
Maryville TN
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[Histonet] Alpha A Crystallin Immuno

[Carrie Schray]

Hello
One of my vets would like to run Alpha A Crystallin IHC on rabbit eyes.
Anyone out there tried this? Any recommendations?

Thank you,
Carrie Schray
Univ. of Michigan Medical School
Ann Arbor, MI
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RE: [Histonet] Leica special/h&e stainer

[Barbara Tibbs]

I have the Leica Multistainer.  It was purchased before I got here.  The only 
thing I don't like is that the programs have to be "compatible" or else the 
machine alarms when you load a special stain onto the machine when it already 
has an H&E going.  There's a rather complicated way to get the programs to be 
compatible which was explained to me when I went for training.  You have to use 
an excel spreadsheet and figure out what rinse is where during all the 
programs.  Yes, it's something Leica's engineers have to work on.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Deloris Carter 

Sent: Monday, June 09, 2014 5:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Leica special/h&e stainer

Does anyone have any feedback on the Leica Multistainer? It stains H&E as
well as special stains.

Thanks,
Deloris Carter, HT(ASCP)
Shawnee Mission Medical Center
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Re: [Histonet] Leica special/h&e stainer

[Cristi]

The ST 5020?  We have that machine and do like it.  We are however a rather 
small volume lab with limited stain needs.  I think if you are looking for a 
variety of staining protocols you should ask what the estimated time is if they 
are all running at the same time.  We only do a dewax, H&E and DQ stain and we 
have to place the racks strategically if we are running behind.  You can "dip" 
yourself into lengthy runs and long holding times in stations if you aren't 
careful.  Mechanically it has been running with no issues for five years now.  
Please let me know if you have other more specific questions.
Have a great day,
Cristi

Sent from my iPhone

> On Jun 9, 2014, at 11:48 AM, Deloris Carter  wrote:
> 
> Does anyone have any feedback on the Leica Multistainer? It stains H&E as
> well as special stains.
> 
> Thanks,
> Deloris Carter, HT(ASCP)
> Shawnee Mission Medical Center
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[Histonet] Leica special/h&e stainer

[Deloris Carter]

Does anyone have any feedback on the Leica Multistainer? It stains H&E as
well as special stains.

Thanks,
Deloris Carter, HT(ASCP)
Shawnee Mission Medical Center
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[Histonet] Equipment for Sale

[Amspacher, September]

I have a Microm HM550vp cryostat for sale.
Please contact me if interested - vendor contacts welcome


Victoria Spoon
Anatomic Pathology Manager
Point Of Care Manager
victoria.sp...@bassett.org
Tel(607) 547-6357
Fax(607) 547-3203


September Amspacher HT(ASCP)


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[Histonet] RE: GI biopsies and special stains

[Horn, Hazel V]

We do the same as Andrea.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bilger, Andrea
Sent: Monday, June 09, 2014 12:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI biopsies and special stains

Tests need to be medically necessary, therefore, we do not auto order stains on 
all GI biopsies.  We wait until the H&E is screened and then order H Pylori by 
IHC on only those biopsies that need it.

Andrea Bilger, HTL
Team Leader, Histology
York Hospital
1001 S. George St.
York, Pa.  17405
(717) 851-5040


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[Histonet] RE: Prisma stainer

[Sharon Scalise]

I am wondering what program you are using for a routine H&E stain.  When are 
stainer was set-up we were told that each station had to be at least 2 minutes; 
if less than that the arm would "babysit" at a station until it moved it to a 
station that was 2 minutes or longer.  This added about 12 minutes to our stain 
protocol and when we are busy in the morning it really slows down the staining 
runs.  We are having a real issue with this so we are going to test the stainer 
with shorter times; if others already have shorter protocols that work could 
you please share them.
(We stain between 600 and 1,000 slides per day)

Sharon Scalise


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent: Monday, June 09, 2014 1:11 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Prisma stainer

We have had the Prisma stainer for 2 years and LOVE it.  Have never had a 
breakdown and it is a workhorse.  It is user friendly and we do not have to use 
proprietary reagents, our choice!

Dorothy Webb
Regions Hospital Histology Technical Specialist

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Monday, June 09, 2014 12:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 127, Issue 12

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
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When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


Today's Topics:

   1. Re: DAB away alternative (Dario Ricca)
   2. G.I. biopsies and special stains (kathy.mach...@lpnt.net)
   3. Re: G.I. biopsies and special stains (Michael Ann Jones)
   4. RE: G.I. biopsies and special stains (Hannen, Valerie)
   5. Re: G.I. biopsies and special stains (Sue)
   6. RE: G.I. biopsies and special stains (Tom McNemar)
   7. RE: G.I. biopsies and special stains (Boyd, Debbie M)
   8. RE: G.I. biopsies and special stains (Mike Pence)
   9. Re: RE: G.I. biopsies and special stains (Victoria Baker)
  10. Re: G.I. biopsies and special stains (Michael Ann Jones)
  11. Documenting special stain control blocks (Histology)
  12. Special Stainers (Adesupo, Adesuyi (Banjo))
  13. FW:H & E Stainers (Adesupo, Adesuyi (Banjo))
  14. Re: Special Stainers (Rene J Buesa)
  15. RE: Special Stainers (Barbara Tibbs)
  16. RE: Special Stainers (Cooper, Brian)


--

Message: 1
Date: Mon, 9 Jun 2014 12:02:16 +0200
From: Dario Ricca 
Subject: Re: [Histonet] DAB away alternative
To: histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset=UTF-8

Hi Thomas,
I've see that Dako's DAB Away is composed of 1% Oxalic acid in water instead 
other DAB Aways (like the one you are using) are composed of sulfuric acid <10% 
in water.

Dario


2014-06-06 17:53 GMT+02:00 Thomas Pier :

> Hello Histonet,
> We have been using Lab Vision's DAB away to clean our Autostainer 360.
> However it seems a little wasteful to buy something that we can't use 
> up before it expires. Do any of you have any exerpience with lower 
> cost alternatives?
>
> Thanks,
> Tom
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


--

Message: 2
Date: Mon, 9 Jun 2014 08:14:42 -0500
From: 
Subject: [Histonet] G.I. biopsies and special stains
To: 
Message-ID:



Content-Type: text/plain; charset="us-ascii"

I was wondering if other labs are "automatically" ordering special stains 
and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait until 
H&E is screened?  Thanks!

Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.net
434-799-3868



--

Message: 3
Date: Mon, 9 Jun 2014 13:37:42 +
From: Michael Ann Jones 
Subject: Re: [Histonet] G.I. biopsies and special stains
To: "kathy.mach...@lpnt.net" ,
"histonet@lists.utsouthwestern.edu"

Message-ID: 
Content-Type: text/plain; charset="us-ascii"

We order HP by IHC automatically on all stomach and GE juction specimens.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/9/14 7:14 AM, "kathy.mach...@lpnt.net"  wrote:

>I was wondering if other labs are "automatically" ordering spe

[Histonet] RE: GI biopsies and special stains

[Barbara Tibbs]

I agree.  However, if you only receive a few GI biopsies a day that might me 
feasible.  95% of my work is GI biopsies.  I live in an area with a very large 
immigrant population which tends to have h.pylori infection (from what I've 
seen).  On any given day over 50% of the gastric biopsies could be h.pylori 
positive.  We see pre-ordering special stains as being efficient and saving the 
pathologist some time as well as increasing turnaround time.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Bilger, Andrea 

Sent: Monday, June 09, 2014 4:50 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI biopsies and special stains

Tests need to be medically necessary, therefore, we do not auto order stains on 
all GI biopsies.  We wait until the H&E is screened and then order H Pylori by 
IHC on only those biopsies that need it.

Andrea Bilger, HTL
Team Leader, Histology
York Hospital
1001 S. George St.
York, Pa.  17405
(717) 851-5040


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[Histonet] GI biopsies and special stains

[Bilger, Andrea]

Tests need to be medically necessary, therefore, we do not auto order stains on 
all GI biopsies.  We wait until the H&E is screened and then order H Pylori by 
IHC on only those biopsies that need it.

Andrea Bilger, HTL
Team Leader, Histology
York Hospital
1001 S. George St.
York, Pa.  17405
(717) 851-5040


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[Histonet] RE: re: Documenting special stain control blocks

[Barbara Tibbs]

I did the same thing.  We've had no problem passing inspections.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of kathy.mach...@lpnt.net 

Sent: Monday, June 09, 2014 4:21 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] re: Documenting special stain control blocks

I made up a form for the special stain/IHC controls for this that lists 
antibody/special stain, type of tissue, Case #, and date put in use. The 
pathologist looks at one stained slide, which is kept with the controls, and 
signs off on form.  I hope this works!!!
Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.net
434-799-3868

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[Histonet] re: Documenting special stain control blocks

[Kathy.Machado]

I made up a form for the special stain/IHC controls for this that lists 
antibody/special stain, type of tissue, Case #, and date put in use. The 
pathologist looks at one stained slide, which is kept with the controls, and 
signs off on form.  I hope this works!!!
Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.net
434-799-3868

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[Histonet] Prisma stainer

[Webb, Dorothy L]

We have had the Prisma stainer for 2 years and LOVE it.  Have never had a 
breakdown and it is a workhorse.  It is user friendly and we do not have to use 
proprietary reagents, our choice!

Dorothy Webb
Regions Hospital Histology Technical Specialist

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Monday, June 09, 2014 12:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 127, Issue 12

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


Today's Topics:

   1. Re: DAB away alternative (Dario Ricca)
   2. G.I. biopsies and special stains (kathy.mach...@lpnt.net)
   3. Re: G.I. biopsies and special stains (Michael Ann Jones)
   4. RE: G.I. biopsies and special stains (Hannen, Valerie)
   5. Re: G.I. biopsies and special stains (Sue)
   6. RE: G.I. biopsies and special stains (Tom McNemar)
   7. RE: G.I. biopsies and special stains (Boyd, Debbie M)
   8. RE: G.I. biopsies and special stains (Mike Pence)
   9. Re: RE: G.I. biopsies and special stains (Victoria Baker)
  10. Re: G.I. biopsies and special stains (Michael Ann Jones)
  11. Documenting special stain control blocks (Histology)
  12. Special Stainers (Adesupo, Adesuyi (Banjo))
  13. FW:H & E Stainers (Adesupo, Adesuyi (Banjo))
  14. Re: Special Stainers (Rene J Buesa)
  15. RE: Special Stainers (Barbara Tibbs)
  16. RE: Special Stainers (Cooper, Brian)


--

Message: 1
Date: Mon, 9 Jun 2014 12:02:16 +0200
From: Dario Ricca 
Subject: Re: [Histonet] DAB away alternative
To: histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset=UTF-8

Hi Thomas,
I've see that Dako's DAB Away is composed of 1% Oxalic acid in water instead 
other DAB Aways (like the one you are using) are composed of sulfuric acid <10% 
in water.

Dario


2014-06-06 17:53 GMT+02:00 Thomas Pier :

> Hello Histonet,
> We have been using Lab Vision's DAB away to clean our Autostainer 360.
> However it seems a little wasteful to buy something that we can't use
> up before it expires. Do any of you have any exerpience with lower
> cost alternatives?
>
> Thanks,
> Tom
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


--

Message: 2
Date: Mon, 9 Jun 2014 08:14:42 -0500
From: 
Subject: [Histonet] G.I. biopsies and special stains
To: 
Message-ID:



Content-Type: text/plain; charset="us-ascii"

I was wondering if other labs are "automatically" ordering special stains 
and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait until 
H&E is screened?  Thanks!

Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.net
434-799-3868



--

Message: 3
Date: Mon, 9 Jun 2014 13:37:42 +
From: Michael Ann Jones 
Subject: Re: [Histonet] G.I. biopsies and special stains
To: "kathy.mach...@lpnt.net" ,
"histonet@lists.utsouthwestern.edu"

Message-ID: 
Content-Type: text/plain; charset="us-ascii"

We order HP by IHC automatically on all stomach and GE juction specimens.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/9/14 7:14 AM, "kathy.mach...@lpnt.net"  wrote:

>I was wondering if other labs are "automatically" ordering special
>stains and/or IHC on stomach biopsies and esophageal biopsies?  Or do
>you wait until H&E is screened?  Thanks!
>
>Danville Regional Medical Center
>Danville, VA
>kathy.mach...@lpnt.net
>434-799-3868
>
>___
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet




--

Message: 4
Date: Mon, 9 Jun 2014 09:42:23 -0400
From: "Hannen, Valerie" 
Subject: [Histonet] RE: G.I. biopsies and special stains
To: "'kathy.mach...@lpnt.net'" ,
"histonet@lists.utsouthwestern.edu"

Message-ID: <450B7A81EDA0C54E97C53D60F00776C3232B4BB793@isexstore03>
Content-Type: text/plain; charset="us-ascii"

We automatically order CD3 and ALC BL/PAS on small bowel,  ALC/BL PAS on 
Barrett's esophagus. H.P. on stomach. Per our Pathologist's order.

Val

RE: [Histonet] Special Stainers

[Cooper, Brian]

The Ventana Symphony is undoubtedly the easiest stainer to use and maintain.  
You will never have an employee put an incorrect reagent into a container, and 
direct to drain waste makes it an appealing option.  The H&E's are absolutely 
beautiful!  Symphony is finicky about certain things though--the type of 
alcohol you use, the humidity levels in your lab, and the unit just doesn't 
like charged slides.  Bubbles in coverslips and slides sticking together when 
filing may or may not be an issue.  That being said, I've used Prisma's 
predecessors (the DRS 2000) and, no matter what, those things NEVER failed.  We 
had three of them that ran 50-70 runs per day for around 10 years, and I can 
count on one hand the number of times they weren't functional.  These are open 
container stainers though, and do require diligent maintenance and reagent 
changes/rotations.  If you can handle that, I'd say go with the Prisma.  

Thanks, 

Brian


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Monday, June 09, 2014 9:01 AM
To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu; Rene J Buesa
Subject: RE: [Histonet] Special Stainers

You cannot go wrong with the Prisma.  I used that in another lab and it was 
wonderful.  

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Rene J Buesa 

Sent: Monday, June 09, 2014 2:53 PM
To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Special Stainers

Prisma every day
René J.


On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" 
 wrote:




Hi Guys,
Please we are in the process of buying a new Special Stainer and 
will appreciate it, if you guys could share your
experience(s) with me. The two stainers we are still looking at are stated 
below;


1.  Symphony by ventana



1.  TissueTek  Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper

Please let me know whether you guys have had any experience with either of the 
equipments or both.

Thanks,

  Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145

==
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RE: [Histonet] Special Stainers

[Barbara Tibbs]

You cannot go wrong with the Prisma.  I used that in another lab and it was 
wonderful.  

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Rene J Buesa 

Sent: Monday, June 09, 2014 2:53 PM
To: Adesupo, Adesuyi (Banjo); histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Special Stainers

Prisma every day
René J.


On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" 
 wrote:




Hi Guys,
Please we are in the process of buying a new Special Stainer and 
will appreciate it, if you guys could share your
experience(s) with me. The two stainers we are still looking at are stated 
below;


1.  Symphony by ventana



1.  TissueTek  Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper

Please let me know whether you guys have had any experience with either of the 
equipments or both.

Thanks,

  Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145

==
CONFIDENTIALITY NOTICE:

This e-mail communication and any attachments may
contain confidential and privileged information for the use
of the designated recipients named above. If you are not
the intended recipient, you are hereby notified that you
have received this communication in error and that any
review, disclosure, dissemination, distribution, or copying
of it or its contents is prohibited. If you have received
this communication in error, please notify the sender
immediately and destroy all copies of this communication
and any attachments.
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Re: [Histonet] Special Stainers

[Rene J Buesa]

Prisma every day
René J. 


On Monday, June 9, 2014 11:03 AM, "Adesupo, Adesuyi (Banjo)" 
 wrote:
  



Hi Guys,
            Please we are in the process of buying a new Special Stainer and 
will appreciate it, if you guys could share your
experience(s) with me. The two stainers we are still looking at are stated 
below;


1.  Symphony by ventana



1.  TissueTek  Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper

Please let me know whether you guys have had any experience with either of the 
equipments or both.

Thanks,

  Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145

==
CONFIDENTIALITY NOTICE:

This e-mail communication and any attachments may 
contain confidential and privileged information for the use 
of the designated recipients named above. If you are not 
the intended recipient, you are hereby notified that you 
have received this communication in error and that any 
review, disclosure, dissemination, distribution, or copying 
of it or its contents is prohibited. If you have received 
this communication in error, please notify the sender 
immediately and destroy all copies of this communication 
and any attachments.
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[Histonet] FW:H & E Stainers

[Adesupo, Adesuyi (Banjo)]

From: Adesupo, Adesuyi (Banjo)
Sent: Monday, June 09, 2014 10:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: Special Stainers


Hi Guys,
Please we are in the process of buying a new H & E Stainers and 
will appreciate it, if you guys could share your
experience(s) with me. The two stainers we are still looking at are stated 
below;


 1.  Symphony by ventana



 1.   TissueTek  Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper

Please let me know whether you guys have had any experience with either of the 
equipments or both.

Thanks,

  Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145

==
CONFIDENTIALITY NOTICE:

This e-mail communication and any attachments may 
contain confidential and privileged information for the use 
of the designated recipients named above. If you are not 
the intended recipient, you are hereby notified that you 
have received this communication in error and that any 
review, disclosure, dissemination, distribution, or copying 
of it or its contents is prohibited. If you have received 
this communication in error, please notify the sender 
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[Histonet] Special Stainers

[Adesupo, Adesuyi (Banjo)]

Hi Guys,
Please we are in the process of buying a new Special Stainer and 
will appreciate it, if you guys could share your
experience(s) with me. The two stainers we are still looking at are stated 
below;


 1.  Symphony by ventana



 1.   TissueTek  Prisma(r)/Glas(tm) g2 Automated Slide Stainer and Coverslipper

Please let me know whether you guys have had any experience with either of the 
equipments or both.

Thanks,

  Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS
  Histology Supervisor
  Norman Regional Health System,
  Norman, OK 73071.
  Tel: 405- 307- 1145

==
CONFIDENTIALITY NOTICE:

This e-mail communication and any attachments may 
contain confidential and privileged information for the use 
of the designated recipients named above. If you are not 
the intended recipient, you are hereby notified that you 
have received this communication in error and that any 
review, disclosure, dissemination, distribution, or copying 
of it or its contents is prohibited. If you have received 
this communication in error, please notify the sender 
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and any attachments.
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[Histonet] Documenting special stain control blocks

[Histology]

Hi All,

Just wondering how everyone is handling the new checklist question ANP. 21450?  
How are you documenting results of verified special stain tissue blocks?

Thanks for any help,
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Re: [Histonet] G.I. biopsies and special stains

[Michael Ann Jones]

We bill all of the stomach HP, but we do not bill all of the GE specimens.
Only those deemed necessary (if we even do them - some pathologists do not
want them, some do)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/9/14 8:30 AM, "Rathborne, Toni"  wrote:

>We wait for H&E.
>For the labs that order automatically, do you bill all of them, or just
>the ones determined to be necessary?
>
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
>Ann Jones
>Sent: Monday, June 09, 2014 9:38 AM
>To: kathy.mach...@lpnt.net; histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] G.I. biopsies and special stains
>
>We order HP by IHC automatically on all stomach and GE juction specimens.
>Michael Ann Jones, HT (ASCP)
>Histology Manager
>Metropath
>7444 W. Alaska Dr. #250
>Lakewood, CO 80226
>303.634.2511
>mjo...@metropath.com
>
>
>
>
>On 6/9/14 7:14 AM, "kathy.mach...@lpnt.net" 
>wrote:
>
>>I was wondering if other labs are "automatically" ordering special
>>stains and/or IHC on stomach biopsies and esophageal biopsies?  Or do
>>you wait until H&E is screened?  Thanks!
>>
>>Danville Regional Medical Center
>>Danville, VA
>>kathy.mach...@lpnt.net
>>434-799-3868
>>
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>
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Re: [Histonet] RE: G.I. biopsies and special stains

[Victoria Baker]

AKA - managed care.  Emphasis on managed.
On Jun 9, 2014 10:11 AM, "Mike Pence"  wrote:

> You gotta love Obamacare! Where "Legal meets Medical".
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
> Sent: Monday, June 09, 2014 9:05 AM
> To: kathy.mach...@lpnt.net; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: G.I. biopsies and special stains
>
> We have just discontinued this practice after Jane Pine Wood (renowned
> Healthcare Attorney and Medical Director for Palmetto Medicare Part B
> coving NC, SC, VA, and WV) stated "ordering special stains prior to review
> of the H&E stain is not reasonable and necessary".   According to the
> pathologist billing company ,there is in an article they have privy to with
> wording which threatens "additional action" taken for providers whose
> ordering of special stains exceeds the 20th percentile of Gastric Pathology
> cases.  For this reason we are now retro ordering special stains on gastric
> biopsies.  We are also looking at IHC necessity.
>
> Debbie M. Boyd HT (ASCP) l Chief Histologist  l Southside Regional Medical
> Center l  200 Medical Park Blvd.  l  Petersburg, Va.  23805 l  PH
> 804-765-5050 l  FAX 804-765-8852
>
> 
> From: histonet-boun...@lists.utsouthwestern.edu [
> histonet-boun...@lists.utsouthwestern.edu] on behalf of
> kathy.mach...@lpnt.net [kathy.mach...@lpnt.net]
> Sent: Monday, June 09, 2014 9:14 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] G.I. biopsies and special stains
>
> I was wondering if other labs are "automatically" ordering special stains
> and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait
> until H&E is screened?  Thanks!
>
> Danville Regional Medical Center
> Danville, VA
> kathy.mach...@lpnt.net
> 434-799-3868
>
> ___
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>
> --
>
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> intended only for the use of the individual(s) and entity named in the
> message. If you are not an intended recipient of this message, please
> notify the sender immediately and delete the material from your computer.
> Do not deliver, distribute or copy this message and do not disclose its
> contents or take any action in reliance on the information it contains.
>
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[Histonet] RE: G.I. biopsies and special stains

[Mike Pence]

You gotta love Obamacare! Where "Legal meets Medical".

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyd, Debbie M
Sent: Monday, June 09, 2014 9:05 AM
To: kathy.mach...@lpnt.net; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: G.I. biopsies and special stains

We have just discontinued this practice after Jane Pine Wood (renowned 
Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving 
NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E 
stain is not reasonable and necessary".   According to the pathologist billing 
company ,there is in an article they have privy to with wording which threatens 
"additional action" taken for providers whose ordering of special stains 
exceeds the 20th percentile of Gastric Pathology cases.  For this reason we are 
now retro ordering special stains on gastric biopsies.  We are also looking at 
IHC necessity.

Debbie M. Boyd HT (ASCP) l Chief Histologist  l Southside Regional Medical 
Center l  200 Medical Park Blvd.  l  Petersburg, Va.  23805 l  PH 804-765-5050 
l  FAX 804-765-8852


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of kathy.mach...@lpnt.net 
[kathy.mach...@lpnt.net]
Sent: Monday, June 09, 2014 9:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] G.I. biopsies and special stains

I was wondering if other labs are "automatically" ordering special stains 
and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait until 
H&E is screened?  Thanks!

Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.net
434-799-3868

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Re: [Histonet] G.I. biopsies and special stains

[Sue]

We auto do hp on gastric.  Anything else is ordered by path.  u need to be 
careful with ins. if neg the may not re-emburse.  

Sent from my iPhone

> On Jun 9, 2014, at 9:14 AM,  wrote:
> 
> I was wondering if other labs are "automatically" ordering special stains 
> and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait until 
> H&E is screened?  Thanks!
> 
> Danville Regional Medical Center
> Danville, VA
> kathy.mach...@lpnt.net
> 434-799-3868
> 
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[Histonet] RE: G.I. biopsies and special stains

[Tom McNemar]

H. Pylori by IHC on all stomach biopsies and Alcian Blue on all esophageal 
biopsies done automatically.

Tom McNemar, HT(ASCP)
Histology Supervisor
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
kathy.mach...@lpnt.net
Sent: Monday, June 09, 2014 9:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] G.I. biopsies and special stains

I was wondering if other labs are "automatically" ordering special stains 
and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait until 
H&E is screened?  Thanks!

Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.net
434-799-3868

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This e-mail, including attachments, is intended for the sole use of the 
individual and/or entity to whom it is addressed, and contains information from 
Licking Memorial Health Systems which is confidential or privileged. If you are 
not the intended recipient, nor authorized to receive for the intended 
recipient, be aware that any disclosure, copying, distribution or use of the 
contents of this e-mail and attachments is prohibited. If you have received 
this in error, please advise the sender by reply e-mail and delete the message 
immediately. You may also contact the LMH Process Improvement Center at 
740-348-4641. E-mail transmissions cannot be guaranteed to be secure or 
error-free as information could be intercepted, corrupted, lost, destroyed, 
arrive late or incomplete, or contain viruses. The sender therefore does not 
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which arise as a result of e-mail transmission. Thank you.

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[Histonet] RE: G.I. biopsies and special stains

[Boyd, Debbie M]

We have just discontinued this practice after Jane Pine Wood (renowned 
Healthcare Attorney and Medical Director for Palmetto Medicare Part B coving 
NC, SC, VA, and WV) stated "ordering special stains prior to review of the H&E 
stain is not reasonable and necessary".   According to the pathologist billing 
company ,there is in an article they have privy to with wording which threatens 
"additional action" taken for providers whose ordering of special stains 
exceeds the 20th percentile of Gastric Pathology cases.  For this reason we are 
now retro ordering special stains on gastric biopsies.  We are also looking at 
IHC necessity.

Debbie M. Boyd HT (ASCP) l Chief Histologist  l Southside Regional Medical 
Center l  200 Medical Park Blvd.  l  Petersburg, Va.  23805 l  PH 804-765-5050 
l  FAX 804-765-8852


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of kathy.mach...@lpnt.net 
[kathy.mach...@lpnt.net]
Sent: Monday, June 09, 2014 9:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] G.I. biopsies and special stains

I was wondering if other labs are "automatically" ordering special stains 
and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait until 
H&E is screened?  Thanks!

Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.net
434-799-3868

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are not an intended recipient of this message, please notify the sender 
immediately and delete the material from your computer. Do not deliver, 
distribute or copy this message and do not disclose its contents or take any 
action in reliance on the information it contains.

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[Histonet] RE: G.I. biopsies and special stains

[Hannen, Valerie]

We automatically order CD3 and ALC BL/PAS on small bowel,  ALC/BL PAS on 
Barrett's esophagus. H.P. on stomach. Per our Pathologist's order.

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.han...@parrishmed.com






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
kathy.mach...@lpnt.net
Sent: Monday, June 09, 2014 9:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] G.I. biopsies and special stains

I was wondering if other labs are "automatically" ordering special stains 
and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait until 
H&E is screened?  Thanks!

Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.net
434-799-3868

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Re: [Histonet] G.I. biopsies and special stains

[Michael Ann Jones]

We order HP by IHC automatically on all stomach and GE juction specimens.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 6/9/14 7:14 AM, "kathy.mach...@lpnt.net"  wrote:

>I was wondering if other labs are "automatically" ordering special stains
>and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait
>until H&E is screened?  Thanks!
>
>Danville Regional Medical Center
>Danville, VA
>kathy.mach...@lpnt.net
>434-799-3868
>
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[Histonet] G.I. biopsies and special stains

[Kathy.Machado]

I was wondering if other labs are "automatically" ordering special stains 
and/or IHC on stomach biopsies and esophageal biopsies?  Or do you wait until 
H&E is screened?  Thanks!

Danville Regional Medical Center
Danville, VA
kathy.mach...@lpnt.net
434-799-3868

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Re: [Histonet] DAB away alternative

[Dario Ricca]

Hi Thomas,
I've see that Dako's DAB Away is composed of 1% Oxalic acid in water
instead other DAB Aways (like the one you are using) are composed of
sulfuric acid <10% in water.

Dario


2014-06-06 17:53 GMT+02:00 Thomas Pier :

> Hello Histonet,
> We have been using Lab Vision's DAB away to clean our Autostainer 360.
> However it seems a little wasteful to buy something that we can't use up
> before it expires. Do any of you have any exerpience with lower cost
> alternatives?
>
> Thanks,
> Tom
>
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