[Histonet] Histotechnologist Job Opening Immediately - Plum Island, USDA

[Arzt, Jonathan]

A single permanent, federal job opening for a research Histotechnologist/Lab 
Manager is now open immediately for application.

The position is within the Agricultural Research Service, USDA at the Plum 
Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY 
or Old Saybrook, CT.
Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400
Salary $44,175.00 to $84,990.00 / Per year commensurate with experience. 
Federal  benefits package.









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[Histonet] BrDU

[Amos Brooks]

Hi,
  I use Sigma's B2531 with 1 hr pretreatment in 1N HCl at 40 deg (in a
coplin jar in my waterbath) then 15 min Trypsin (don't forget the CaCl2) at
40 deg. I detect it witb Envision+ but any mouse IgG would work fine.

Amos

Message: 15
Date: Tue, 24 Jun 2014 15:12:01 +
From: "Chiriboga, Luis" 
Subject: [Histonet] BrDU
To: " (Histonet@lists.utsouthwestern.edu)"

Message-ID:
<3e6798f00c9f494399e96b720ecd14292b2...@msgwcdcpmb22.nyumc.org>
Content-Type: text/plain; charset="us-ascii"

Hi Histonetters
Can anyone recommend a good anti-BrDU antibody and protocol

Thanks  & Happy Summer!!
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[Histonet] Histotechnologist Opening Immediately - Plum Island, USDA

[Arzt, Jonathan]

A single permanent, federal job opening for a research Histotechnologist/Lab 
Manager is now open immediately for application.

The position is within the Agricultural Research Service, USDA at the Plum 
Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY 
or Old Saybrook, CT.
Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400
Salary $44,175.00 to $84,990.00 / Per year commensurate with experience. 
Federal  benefits package.








This electronic message contains information generated by the USDA solely for 
the intended recipients. Any unauthorized interception of this message or the 
use or disclosure of the information it contains may violate the law and 
subject the violator to civil or criminal penalties. If you believe you have 
received this message in error, please notify the sender and delete the email 
immediately.
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[Histonet] Posting for a HIstonet member- Position available

[Sandy Cope-yokoyama]

If you're looking for a friendly environment, a new state-of-the-art facility 
and an employer of choice, SPL is the place for you!! We currently have several 
openings:



* Histotechnicians/Histotechnologists   Histo candidates should be an 
HTL or HT (ASCP) or equivalent.  Primary responsibilities include on-site 
frozen sections including mobile laboratory units.

* Lab Aide

* Vehicle Service Coordinator



All candidates must have a valid drivers license and proof of insurability.





SPL is CAP accredited, offers competitive pay, a comprehensive benefits 
package.  SPL pays 100% of employee premiums for  Medical, Dental, LTD and 
Life.  We also offer Retirement Plan & Supplemental Insurance.



Please forward resumes to 
ta...@surgicalpathlabs.com

8455 66th Street N

Pinellas Park,  Fl   33781

Fax - 727-545-1644




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intended recipient, any
disclosure, copying, printing, or use of this information is strictly 
prohibited and possibly a
violation of federal or state law and regulations. If you have received this 
information in error,
please notify Children's Medical Center Dallas immediately at 214-456- or 
via e-mail at
priv...@childrens.com. Children's Medical Center Dallas and its affiliates 
hereby claim all
applicable privileges related to this information.


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intended recipient, any
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information in error,
please notify Children's Medical Center Dallas immediately at 214-456- or 
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hereby claim all
applicable privileges related to this information.

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[Histonet] RE: BrDU

[Bea DeBrosse-Serra]

Hi Luis, 

We are using the BrDU In-Situ Detection Kit #551321 from BD Biosciences with 
good luck.

Bea

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis
Sent: Tuesday, June 24, 2014 8:55 AM
To: (Histonet@lists.utsouthwestern.edu)
Subject: [Histonet] RE: BrDU

Apologies, I meant to add for FFPE muse/rat tissue TX's

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis
Sent: Tuesday, June 24, 2014 11:12 AM
To: (Histonet@lists.utsouthwestern.edu)
Subject: [Histonet] BrDU

Hi Histonetters
Can anyone recommend a good anti-BrDU antibody and protocol

Thanks  & Happy Summer!!

This email message, including any attachments, is for the sole use of the 
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[Histonet] Re: Slides for thermo Slidemate

[Teri Johnson]

Those of you having trouble with slides in the Slidemate, please respond to me 
privately and let me know which ones are causing trouble for you.

Thanks!

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752

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Re: [Histonet] Slides for Thermo Slidemate

[Patrick Laurie]

Cancer Diagnostics has an Autofrost clipped corner that completely
eliminated our slide jamming issues and doesn't smear. We have 7 of these
printers and have had no issue.  They have multiple colors,and also have a
good silanized Autofrost IHC slides (we get the 90 degree corner for our
machines) that does has behaved very well in our Slidemates..

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869


On Mon, Jun 23, 2014 at 3:37 PM, Laurie Colbert 
wrote:

> What slides are others using (other than Thermo's Superfrost Plus slides)
> that won't jam in the Slidemate and that will produce print that does not
> smear?
>
> Laurie Colbert, HT (ASCP)
> Histology Supervisor
> PATH MD
> 8158 Beverly Blvd.
> Los Angeles, CA  90048
> (323) 648-3214 direct
> (424) 245-7284 main lab
>
> The information contained in this transmission may contain privileged and
> confidential information, including patient information protected by
> federal and state privacy laws. It is intended only for the use of the
> person(s) named above. If you are not the intended recipient, you are
> hereby notified that any review, dissemination, distribution, or
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[Histonet] RE: BrDU

[Chiriboga, Luis]

Apologies, I meant to add for FFPE muse/rat tissue
TX's

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis
Sent: Tuesday, June 24, 2014 11:12 AM
To: (Histonet@lists.utsouthwestern.edu)
Subject: [Histonet] BrDU

Hi Histonetters
Can anyone recommend a good anti-BrDU antibody and protocol

Thanks  & Happy Summer!!

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intended recipient(s) and may contain information that is proprietary, 
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RE: [Histonet] Eosin Leaching

[Michael LaFriniere]

I have seen this happen with high levels of Isopropanol in alcohols after the 
eosins, I switch to regular reagent alcohols  and seemed to eliminate the 
bleeding of the eosin.

Michael
Michael R. LaFriniere, HT (ASCP) 
Executive Director
 

Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A * Silver Spring, MD 20904  
P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844
michael.lafrini...@ccplab.com
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne 
Anderson
Sent: Thursday, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching

Hello all,

We're having a problem with our eosin bleeding out of the sections. I've read 
that this can be caused by not dehydrating adequately after eosin, but we're 
still having an issue when using fresh alcohols. Does anyone have any other 
ideas as to the cause of this problem?

Thanks in advance for your help!

Adrienne



Adrienne Anderson, BS, HTL(ASCP)
Histotechnologist
Phylogeny, Inc. 
1476 Manning Pkwy, Powell, Ohio 43605
Phone: (614) 846-6161 
Fax:  (877) 591-1815

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[Histonet] BrDU

[Chiriboga, Luis]

Hi Histonetters
Can anyone recommend a good anti-BrDU antibody and protocol

Thanks  & Happy Summer!!

This email message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain information that is proprietary, 
confidential, and exempt from disclosure under applicable law. Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you have received 
this email in error please notify the sender by return email and delete the 
original message. Please note, the recipient should check this email and any 
attachments for the presence of viruses. The organization accepts no liability 
for any damage caused by any virus transmitted by this email.
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[Histonet] Can acetic acid in fixative affect fungal staining by PAS?

[Conway, Carla]

Hello,

I fixed skin/muscle and gill tissues for 48 h in Dietrich's fixative (30 mL
95 % ethanol, 10 mL formalin, 2 mL glacial acetic acid and 58 mL deionized
water) and used the Hotchkiss-McManus PAS protocol to stain
*Saprolegnia *fungi.
The fungi are light green from the counterstain and not PAS-positive. I
used fresh staining reagents and am wondering if the acetic acid in the
Dietrich's fixative may have affected the cell walls. I'd appreciate your
comments and suggestions.

Thanks very much!

Carla

Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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RE: [Histonet] need recommendations on working alpha synulcein IHC for human tissue

[Connolly, Brett M]

I would also be interested in what Alpha synuclein Abs people are using for IHC
Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maria Mejia
Sent: Tuesday, June 24, 2014 12:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] need recommendations on working alpha synulcein IHC for 
human tissue

I'm looking for a working alpha synuclein IHC antibody to be used on human 
tissue.  I work on human
brain stem free-floating 60um sections.  I would greatly appreciate any 
suggestions & recommendations
from anyone.

Regards

Maria Mejia
Histology Supervisor
UCSF
Department of Neurology
SF, CA
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