Re: [Histonet] from saline to formalin
Hi Nancy, I found this paper (unfortunately I don't have access to the full article) that may be of help; they looked at various time points for holding the tissue in saline before being fixed in formalin. http://www.ncbi.nlm.nih.gov/pubmed/24822006 J Maxillofac Oral Surg. 2014 Jun;13(2):148-51. doi: 10.1007/s12663-013-0473-z. Epub 2013 Jan 26. Artefacts produced by normal saline when used as a holding solution for biopsy tissues in transit. Sengupta S1, Prabhat K2, Gupta V2, Vij H2, Vij R2, Sharma V2. Cheers, Cecilia On Jun 26, 2014, at 4:55 PM, Nancy Schmitt wrote: > Do you know where there might be a reference for….”How long can a specimen be > in saline until it should be placed in formalin or other fixative?” > > Thank you > > Nancy Schmitt MLT, HT(ASCP) > United Clinical Laboratories > Dubuque, IA 52001 > > > > NOTICE: This email may contain legally privileged information. The information > is for the use of only the intended recipient(s) even if addressed > incorrectly. If you are not the intended recipient, please notify the sender > that you have received it in error and then delete it along with any > attachments. Thank you. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: from saline to formalin
The answer to that question will depend on what you are going to do with the tissue (H&E, IHC, or molecular testing)? Richard Richard W. Cartun, MS, PhD Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Thursday, June 26, 2014 5:55 PM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] from saline to formalin Do you know where there might be a reference for"How long can a specimen be in saline until it should be placed in formalin or other fixative?" Thank you Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] from saline to formalin
Do you know where there might be a reference for….”How long can a specimen be in saline until it should be placed in formalin or other fixative?” Thank you Nancy Schmitt MLT, HT(ASCP) United Clinical Laboratories Dubuque, IA 52001 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Recycle or not?
My condolences, Jim. Yes, that happened to me in my former place of employment. It was not fun. To the companies who provide recycling equipment, it would behoove you to be aware of federal guidelines and how it might not only affect your ability to sell new units, but also how it affects current customers who may have to deal with issues from regulatory agencies. A little bit of guidance in these matters would be quite helpful to us. Best wishes, Teri Johnson Manager, Histology Genomics Institute for Novartis Research Foundation San Diego, CA 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Recycle or not?
I used this exact argument in my defense (as suggested by the manufacturer of my recycler) and the EPA responded with the following: "You should consider discussing your recycling activity with MassDEP (the state agency). Every state has unique interpretations on when a recycling license or permit is required--and the manufacturer of a device is not the best contact to make that decision". Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: James Watson [mailto:jwat...@gnf.org] Sent: Thursday, June 26, 2014 2:19 PM To: 'Mass Histology'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Recycle or not? This topic was discussed at a meeting somewhere that I attended and here with our safety staff. The work around that we do here to conform to waste disposal regulations here in Ca. is that xylene for recycling is not classified as waste, so it does not enter the waste stream. Since you are not recycling waste you are not practicing "waste management". James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mass Histology Sent: Thursday, June 26, 2014 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycle or not? This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Recycle or not?
This topic was discussed at a meeting somewhere that I attended and here with our safety staff. The work around that we do here to conform to waste disposal regulations here in Ca. is that xylene for recycling is not classified as waste, so it does not enter the waste stream. Since you are not recycling waste you are not practicing "waste management". James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Tel 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mass Histology Sent: Thursday, June 26, 2014 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycle or not? This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycled or not? NO PHI
Gotta go with what works!! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: Barbara Tibbs [mailto:barbara.ti...@accuratediagnosticlabs.com] Sent: Thursday, June 26, 2014 11:49 AM To: Weems, Joyce K.; histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI Hmm. Maybe the company who manufactured our recycler went out of business or we got a lemon. As soon as we abandoned recycling and went back to new reagents, the stains and processing were perfect. Go figure. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce K. Sent: Thursday, June 26, 2014 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI I have used recycled xylene since the mid-80s and the only problem is that it is purer than new xylene and can make biopsies crispy. (The isomers get distilled out.) We use new xylene on the biopsy processor. The recycler is in our lab and there are no fumes at all. Surely does save money. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Thursday, June 26, 2014 11:20 AM To: Blazek, Linda; Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol an
RE: [Histonet] RE: Recycle or not?
Well..that's a new one!!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Thursday, June 26, 2014 12:47 PM To: 'Mass Histology'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Recycle or not? Haven't heard this one till now! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mass Histology Sent: Thursday, June 26, 2014 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycle or not? This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ="This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Recycle or not?
Haven't heard this one till now! Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mass Histology Sent: Thursday, June 26, 2014 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycle or not? This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: ISH Automation
Hi - there are a couple of instruments that automate ISH and RISH. Our lab was a beta site for BioCare's Oncore and we loved it. It has an open platform and is very user friendly. The Oncore is a very compact instrument and the maintenance was easy and minimal. All of the techs here liked it. Check it out. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 6 Date: Wed, 25 Jun 2014 08:38:29 -0400 From: Emily Brown Subject: Re: [Histonet] ISH preparation To: jennifer.john...@genzyme.com That may be what you have to do, as the first day of ISH needs to be RNase free! When I do them, it is 10 slides at a time and we have dishes and a lab bench set aside for RNase free procedures only. Obviously this is not feasible in all workplaces! I would just think about it logically. Use gloves when you can, always. Can you keep the hybridizing part RNase free? I don't know what machines you use for this--we do process the slides manually in glass dishes and then hybridize in a slide container and oven kept for RNase free purposes. After hybridization, you don't really need to be too careful. Now I'm wondering if there's some sort of magical machine that does ISH! Emily - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycle or not?
This might be a little off the topic: We recently had the enjoyment of undergoing a day-long EPA inspection and we were issued a warning of "practicing waste management without proper permits" when they saw our recycler. Has anyone else ever been hassled by the EPA regarding this? Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histotechnologist Opening Immediately - Plum Island, USDA
A single permanent, federal job opening for a research Histotechnologist/Lab Manager is now open immediately for application. The position is within the Agricultural Research Service, USDA at the Plum Island Animal Disease Center. Staff commute daily by boat from Orient Point, NY or Old Saybrook, CT. Details at: https://www.usajobs.gov/GetJob/ViewDetails/373522400 Salary $44,175.00 to $84,990.00 / per year commensurate with experience. Federal benefits package. This electronic message contains information generated by the USDA solely for the intended recipients. Any unauthorized interception of this message or the use or disclosure of the information it contains may violate the law and subject the violator to civil or criminal penalties. If you believe you have received this message in error, please notify the sender and delete the email immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycled or not? NO PHI
Hmm. Maybe the company who manufactured our recycler went out of business or we got a lemon. As soon as we abandoned recycling and went back to new reagents, the stains and processing were perfect. Go figure. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce K. Sent: Thursday, June 26, 2014 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI I have used recycled xylene since the mid-80s and the only problem is that it is purer than new xylene and can make biopsies crispy. (The isomers get distilled out.) We use new xylene on the biopsy processor. The recycler is in our lab and there are no fumes at all. Surely does save money. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Thursday, June 26, 2014 11:20 AM To: Blazek, Linda; Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders
RE: [Histonet] RE: Recycled or not? NO PHI
We only recycle xylene which we've tested purity and was close to 98-99% and is used in both H&E staining and processing with no issue. I've done 8 hour xylene vapor monitoring in our recycling room, while recycling was occurring, and the results were 6.0ppm where the OSHA exposure limit is 100.0ppm. Andrew Horvath, MBA, MA Operations Manager 303-770-4848 (O) 303-770-6641 (fax) Colorado GI Pathology 7346 S. Alton Way Suite 10E Centennial, CO 80112 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 9:23 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI They are very fussy as you put it. However, I am even more anal than they are about our work. We do QA checks both before and after we recycle. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: Barbara Tibbs [mailto:barbara.ti...@accuratediagnosticlabs.com] Sent: Thursday, June 26, 2014 10:37 AM To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI Maybe your pathologists aren't as fussy as the pathologists I worked with at the time. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: Podawiltz, Thomas Sent: Thursday, June 26, 2014 1:33 PM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histon
Re: [Histonet] RE: Recycled or not? NO PHI
Hi Joyce, Absolutely agree with recycling concept, value, money saved and no fumes in lab (if using newer models) and if used properly. I've always been curious about the concept of a lab recycler making xylene "purer" by distilling out isomers. Which unit do you have? meta-xylene is in great demand as a feedstock for plastic production. Since xylene(s) are a mixture of ortho-, meta- and para all of which differ in boiling points by just very few degrees, they are (near) impossible to separate out from one another by ordinary distillation and need multi-fractional set-ups with crystallization and absorption and catalytic beds. Manufacturers spend vast sums to do this and are always looking for a better way. What unit do you have? Have you had chromatography done on your (new) input and then output xylene. I've done it extensively for alcohol but never xylene. Thanks, Ray Seattle, WA - Original Message - From: "Joyce K. Weems" To: histonet@lists.utsouthwestern.edu Sent: Thursday, June 26, 2014 8:33:04 AM Subject: [Histonet] RE: Recycled or not? NO PHI I have used recycled xylene since the mid-80s and the only problem is that it is purer than new xylene and can make biopsies crispy. (The isomers get distilled out.) We use new xylene on the biopsy processor. The recycler is in our lab and there are no fumes at all. Surely does save money. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Thursday, June 26, 2014 11:20 AM To: Blazek, Linda; Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic L
[Histonet] RE: Recycled or not? NO PHI
I have used recycled xylene since the mid-80s and the only problem is that it is purer than new xylene and can make biopsies crispy. (The isomers get distilled out.) We use new xylene on the biopsy processor. The recycler is in our lab and there are no fumes at all. Surely does save money. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of McAnn, Sherrian Sent: Thursday, June 26, 2014 11:20 AM To: Blazek, Linda; Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are no
[Histonet] RE: Recycled or not? NO PHI
They are very fussy as you put it. However, I am even more anal than they are about our work. We do QA checks both before and after we recycle. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: Barbara Tibbs [mailto:barbara.ti...@accuratediagnosticlabs.com] Sent: Thursday, June 26, 2014 10:37 AM To: Podawiltz, Thomas; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI Maybe your pathologists aren't as fussy as the pathologists I worked with at the time. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: Podawiltz, Thomas Sent: Thursday, June 26, 2014 1:33 PM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI
We routinely recycle both our alcohols and xylenes. They are checked for purity and with the alcohol the extra step of ensuring that we are getting the correct percentage (95%) recovered. We have never had any issues in any of our processors or stainers since using recycled reagents. We also have not had an issue with fumes. The recyclers nowadays are much better than their older versions and I think that sometimes prejudices come into play with the older techs like me who were around for the older models. P. S. We used to have to do ours on a hotplate with a large round glass ball and would have to clean the ball out. Those were not the good ole days. :) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, June 26, 2014 9:43 AM To: Podawiltz, Thomas; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] RE: Recycled or not? NO PHI I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycled or not? NO PHI
I agree with Tom. With the exception of self-inflicted issues we also have not had any issues with recycling our reagents. We check each batch as it is recycled. We also don't have a problem with fumes. (And our pathologists are fussy) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Podawiltz, Thomas Sent: Thursday, June 26, 2014 10:34 AM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycled or not? NO PHI
Maybe your pathologists aren't as fussy as the pathologists I worked with at the time. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: Podawiltz, Thomas Sent: Thursday, June 26, 2014 1:33 PM To: Barbara Tibbs; Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: RE: Recycled or not? NO PHI We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycled or not? NO PHI
We have never had an issue with either our recycled xylene or alcohol that was not self inflicted. When our system is running there are no fumes. Tom Podawiltz HT (ASCP) Histology Section Head LRGHealthcare Laconia, NH 03246 603-524-3211 ext: 3220 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs Sent: Thursday, June 26, 2014 9:06 AM To: Sanders, Jeanine (CDC/OID/NCEZID); histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Recycled or not? While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Recycled or not?
While I can agree that recycling alcohol and xylene is both environmentally and economically advantageous, technically it's awful. There's no way to make used alcohol and xylene as pure as it was originally. There's also the issue of fumes from recycling a solvent. The company I had used years ago swore that there were no fumes when using their machine but the personnel working in the laboratory would vigorously disagree. Barbara S. Tibbs Histology Supervisor Accurate Diagnostic Labs South Plainfield, NJ barbara.ti...@accuratediagnosticlabs.com 732-839-3374 Cell: 610-809-6508 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 26, 2014 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recycled or not? Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana iScan Coreo Slide Scanner
Does anyone in the United States provide service for the Ventana Coreo Slide Scanner other than Ventana? Many Thanks, Susan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recycled or not?
Morning All! I have heard for years the general problems with using recycled alcohols on H&E stainers, but do the same problems occur when using recycled xylene? Thanks! Jeanine H. Sanders Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 j...@cdc.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
