[Histonet] AP Module of Cerner Millennium + AB&T and AP Module of Cerner Millenium + Cerebro

[Joana Moreira]

Dear all,

I was wondering if there's any one out there using the AP Module of the Cerner 
Millennium LIS with their Advanced Barcode and Tracking (AB&T) solution?
And also, if there's anyone out there using the AP Module of the Cerner 
Millennium LIS interfaced with Leica's Cerebro Specimen tracking system?

Many Thanks,
Joana

Joana Moreira
Supervisor - Anatomical Pathology
Department of Pathology

Sidra Medical & Research Center
PO Box 26999 | Doha, Qatar
Direct Line  +974-4404-2036
jmore...@sidra.org | www.sidra.org






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[Histonet] RE: Decalcifying and Processing large bones

[Cesar Francisco Romero]

For many years now I have used this protocol in Bone
Marrow samples.

The cortical of the bone is still a problem, but it
can be solved with longer decal time.

If you need to preserve morphology and antigenicity
for Immunohistochemistry this is the best choice.

Three Days Bouin’s Fixative ( From the very Beginning
), don’t use Formalin.

Three Days of a Saturated Solution of EDTA ( Disodium
Salt ) in distilled water.

After this time put the blocks in the first alcohol and
process as usual.

Don’t put the blocks in formalin.

EDTA alone or with Heating  affects morphology very badly.

For IHC Antigen Retrieval is the recommended or used
for each antibody.

Difficult ones as CD 5 or CD 23 work very well with
this decal protocol.

Cesar
Romero

Buenos
Aires.

Argentina.

  
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RE: [Histonet] RE: p16

[Weems, Joyce K.]

They bought the company!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ronda Mire
Sent: Wednesday, August 06, 2014 3:37 PM
To: Burnett, Brandy
Cc: histonet@lists.utsouthwestern.edu; sarah.dys...@stdavids.com
Subject: Re: [Histonet] RE: p16

Before Ventana had p16, it was only available from MTM labs, if I recall 
correctly the name. I am not sure if Ventana is the exclusive vendor now or 
not.  Good luck.
On Aug 6, 2014, at 3:13 PM, "Burnett, Brandy"  
wrote:

> I am looking for one as well, I wasn't sure if they owned the patent. Let me 
> know if you find one.
> Thanks!
>
> Brandy Burnett, HTL (ASCP)
> Cape Cod Hospital
>
> 
> From: histonet-boun...@lists.utsouthwestern.edu
> [histonet-boun...@lists.utsouthwestern.edu] on behalf of
> sarah.dys...@stdavids.com [sarah.dys...@stdavids.com]
> Sent: Wednesday, August 06, 2014 10:51 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] p16
>
> Does anyone know of a comparable clone to the Ventana p16 clone E6H4?  I 
> would prefer it to be undiluted (concentrate) so that we can titrate out the 
> antibody ourselves.  We need it to have publications that would back this up 
> and/or stained slide pictures I can show our pathologist.
> Thanks
>
> Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St.
> David's North Austin Medical Center
> 12221 North Mopac Expressway
> Austin, Texas  78758
> (512)901-1220
>
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> ~~
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> solely for the use of the individual or entity to whom they are addressed. If 
> you have received this email in error  contact the Help Desk for Cape Cod 
> Healthcare.
>
> helpd...@capecodhealth.org
>
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Re: [Histonet] RE: p16

[Ronda Mire]

Before Ventana had p16, it was only available from MTM labs, if I recall 
correctly the name. I am not sure if Ventana is the exclusive vendor now or 
not.  Good luck.
On Aug 6, 2014, at 3:13 PM, "Burnett, Brandy"  
wrote:

> I am looking for one as well, I wasn't sure if they owned the patent. Let me 
> know if you find one.
> Thanks!
> 
> Brandy Burnett, HTL (ASCP)
> Cape Cod Hospital
> 
> 
> From: histonet-boun...@lists.utsouthwestern.edu 
> [histonet-boun...@lists.utsouthwestern.edu] on behalf of 
> sarah.dys...@stdavids.com [sarah.dys...@stdavids.com]
> Sent: Wednesday, August 06, 2014 10:51 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] p16
> 
> Does anyone know of a comparable clone to the Ventana p16 clone E6H4?  I 
> would prefer it to be undiluted (concentrate) so that we can titrate out the 
> antibody ourselves.  We need it to have publications that would back this up 
> and/or stained slide pictures I can show our pathologist.
> Thanks
> 
> Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP)
> Pathology Supervisor
> St. David's North Austin Medical Center
> 12221 North Mopac Expressway
> Austin, Texas  78758
> (512)901-1220
> 
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[Histonet] RE: p16

[Weems, Joyce K.]

It is an exclusive Ventana product - sold through MTM Laboratories I believe it 
is.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy
Sent: Wednesday, August 06, 2014 3:14 PM
To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: p16

I am looking for one as well, I wasn't sure if they owned the patent. Let me 
know if you find one.
Thanks!

Brandy Burnett, HTL (ASCP)
Cape Cod Hospital


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
sarah.dys...@stdavids.com [sarah.dys...@stdavids.com]
Sent: Wednesday, August 06, 2014 10:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16

Does anyone know of a comparable clone to the Ventana p16 clone E6H4?  I would 
prefer it to be undiluted (concentrate) so that we can titrate out the antibody 
ourselves.  We need it to have publications that would back this up and/or 
stained slide pictures I can show our pathologist.
Thanks

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's 
North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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[Histonet] RE: p16

[Burnett, Brandy]

I am looking for one as well, I wasn't sure if they owned the patent. Let me 
know if you find one.
Thanks!

Brandy Burnett, HTL (ASCP)
Cape Cod Hospital


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
sarah.dys...@stdavids.com [sarah.dys...@stdavids.com]
Sent: Wednesday, August 06, 2014 10:51 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] p16

Does anyone know of a comparable clone to the Ventana p16 clone E6H4?  I would 
prefer it to be undiluted (concentrate) so that we can titrate out the antibody 
ourselves.  We need it to have publications that would back this up and/or 
stained slide pictures I can show our pathologist.
Thanks

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP)
Pathology Supervisor
St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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Re: [Histonet] RE: Plus slides for Thermo SlideMate

[Grantham, Andrea L - (algranth)]

I use the EPIC slides from CWS, Have always used them on this printer but have 
tried many other brands - these are the BEST.




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algra...@email.arizona.edu
Tel: 520.626.4415 Fax: 520.626.2097
retiring in 30 days!




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[Histonet] RE: Plus slides for Thermo SlideMate

[Walter Benton]

Teri,

We have found that these slides work the best:

Leica
Part # 3800090
SLIDE WHITE Xtra Thermal (Color should not matter, we tried several different 
colors with good results)


Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson 
[tjohn...@gnf.org]
Sent: Wednesday, August 06, 2014 12:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Plus slides for Thermo SlideMate

Dear all,

For those of you using the SlideMate, can you give me a list of compatible 
slides for these units?

Thanks!

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752

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[Histonet] Responses to Plastics

[Hans B Snyder]

Hello All,

Thank you to all who responded to my query about the RI company.  I
have forwarded all your emails directly to the company. Who ever has
the pleasure to service the company, you have my gratitude.

Best

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com

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[Histonet] Plus slides for Thermo SlideMate

[Teri Johnson]

Dear all,

For those of you using the SlideMate, can you give me a list of compatible 
slides for these units?

Thanks!

Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752

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Re: [Histonet] neutralize formalin

[Rene J Buesa]

Maryann:
In spite of any seller's or user's opinion, I tried ALL available formalin 
neutralizers available in the market up to 2002 and was able to detect, after 
the so called "neutralization", significant amounts of free formalin still 
present.
Because of these results I decided to keep sending my used formalin to a 
"disposal" company even when as established by law, MY LAB was responsible of 
any problems with that used formalin even when we did not dispose of it, 
meaning that the disposal companies are NOT liable for any problem. I recommend 
you to look into this "cradle-to-burial" responsibility for your lab. in your 
state.
We recycled our xylene until I developed a tissue processing method using only 
2-propanol and mineral oil instead of ethanol and xylene and all our xylene 
related health problems disappeared with a significant improvement of processed 
tissue quality.
René J.  


On Wednesday, August 6, 2014 11:08 AM, Mary Ann Deathridge 
 wrote:
  


We  pour our waste xylene/alcohol into a drum and the formalin waste in a 
seperate drum for a waste service company to remove.  Problem is space to 
house 55 gal drums and we have more xylene/alc waste than formalin.  Does 
anyone neutralize formalin and dispose of it down the drain?  If so can you 
recommend  the method/product  utilized.
Xylene recyler is not an option.
  
  Maryann Deathridge, BS, HT (ASCP)  Lab Manager  615-298-4100, Fax: 
615-298-4141 




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[Histonet] RE: Decalcifying and Processing large bone

[Huynh,Thomas]

Hi Bret
We are using 10% Formic Acid as well and checking the decalcifying end-point by 
using the faxitron x-ray system. I work with bone cancer for 99% of the time 
and Formic Acid is safer than any other one.

Thomas 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Wednesday, August 06, 2014 10:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 129, Issue 6

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Today's Topics:

   1. RE: Protocol for decalcifying and processing large bone
  section. (DiCarlo, Margaret)
   2. AW: [Histonet] RE: whole slide scanners (Gudrun Lang)
   3. Glassware Cleaning, CAP Checklist Item GEN.41770 (Laurie Colbert)
   4. RE: RE: whole slide scanners (Elizabeth Chlipala)
   5. Listservers in microbiology (Jorge A. Santiago-Blay)
   6. AW: [Histonet] RE: whole slide scanners (Gudrun Lang)
   7. Procedures and Policy manual needed (Dr. Max Mirot)
   8. RE: Glassware Cleaning, CAP Checklist Item GEN.41770
  (Cooper, Brian)
   9. RE: RE: whole slide scanners (Morken, Timothy)
  10. RE: Listservers in microbiology (Cartun, Richard)
  11. RE: RE: whole slide scanners (James Watson)
  12. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770
  (Joelle Weaver)
  13. RE: RE: Glassware Cleaning, CAP Checklist Item GEN.41770 (Jamal)
  14. Re: Protocol for decalcifying and processing large bone
  section. (Barry Rittman)
  15. Re: RE: Glassware Cleaning, CAP Checklist Item GEN.41770
  (Michael Ann Jones)
  16. RI company seeks plastics company for research work
  (Hans B Snyder)
  17. p16 (sarah.dys...@stdavids.com)
  18. neutralize formalin (Mary Ann Deathridge)


--

Message: 1
Date: Tue, 5 Aug 2014 17:01:16 +
From: "DiCarlo, Margaret" 
Subject: RE: [Histonet] Protocol for decalcifying and processing large
bonesection.
To: "'Clough, Bret'" , Histonet list serv.

Message-ID:

<731be09cdb19aa43aa8682199d42d31b1dc79...@adcexchange01.kaleidahealth.org>

Content-Type: text/plain; charset="us-ascii"

I use 10% formic acid for large human bones but I don't think your bone 
sections are that large.

Peggy DiCarlo
Ortho Bone Lab
BGMC
100 High St. 
Buffalo, NY  14203

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clough, Bret
Sent: Tuesday, August 05, 2014 11:55
To: Histonet list serv.
Subject: [Histonet] Protocol for decalcifying and processing large bone section.

Hi everyone,
 I was hoping someone on histonet would being willing to help me by either 
sharing their protocol or advising me on decalcifying and processing large bone 
sections. The bone sections are from the femural head of sheep measuring 
roughly 1cm x 1 1/2 cm cube.  Currently I've been decalcifying the samples in 
0.5M EDTA which is taking along time to decalcify this being the 19th day.  Any 
advise would be greatly appreciated!


Sincerely,
   Bret Clough
   Texas A&M Health Science Center
   Temple, TX.
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of information, stories and pictures about our valued workforce and the 
tremendous momentum our organization is experiencing. Check us out at: 
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[Histonet] neutralize formalin

[Mary Ann Deathridge]

We  pour our waste xylene/alcohol into a drum and the formalin waste in a 
seperate drum for a waste service company to remove.  Problem is space to 
house 55 gal drums and we have more xylene/alc waste than formalin.  Does 
anyone neutralize formalin and dispose of it down the drain?  If so can you 
recommend  the method/product  utilized.
 Xylene recyler is not an option.
  
  Maryann Deathridge, BS, HT (ASCP)  Lab Manager  615-298-4100, Fax: 
615-298-4141 




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[Histonet] p16

[Sarah.Dysart]

Does anyone know of a comparable clone to the Ventana p16 clone E6H4?  I would 
prefer it to be undiluted (concentrate) so that we can titrate out the antibody 
ourselves.  We need it to have publications that would back this up and/or 
stained slide pictures I can show our pathologist.
Thanks

Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP)
Pathology Supervisor
St. David's North Austin Medical Center
12221 North Mopac Expressway
Austin, Texas  78758
(512)901-1220

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[Histonet] RI company seeks plastics company for research work

[Hans B Snyder]

Hello All,

I have a company who needs:  "a source of basic services for
sectioning of GMA blocks containing biomaterial devices and subsequent
H&E staining.  The devices are cut for embedding to about 8mm in
length, and contain adhesives, thermoplastic membranes, various
scaffolds, and cells.  Our group would do the embedding and transfer
samples to you, and would be looking for about 25-50 samples/month."

I would like to give him references so that he can email and or call
places he chooses.  Please email me and I will forward all
information.

Thank you

Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com

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Re: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770

[Michael Ann Jones]

Ditto with Brian Cooper.
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 8/5/14, 1:07 PM, "Cooper, Brian"  wrote:

>I've never been asked by a CAP auditor either, but our internal
>inspectors used to inquire about this in a previous lab all the time.  We
>use Bromocresol Purple as an indicator here, and we have a simple log
>sheet wherein our crew just tests one piece of cleaned glassware each
>day.  They write the type of glassware they test, and place a checkmark
>in a "yellow" or "purple" column based upon what they see.  If "purple,"
>they rewash, retest and document accordingly.
>
>Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
>Department of Pathology and Laboratory Medicine
>Children's Hospital Los Angeles
>4650 Sunset Blvd MS#43- Los Angeles, CA 90027
>Ph: 323.361.3357 Pager: 213-209-0184
>bcoo...@chla.usc.edu
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
>Colbert
>Sent: Tuesday, August 05, 2014 11:06 AM
>To: Histonet Post (histonet@lists.utsouthwestern.edu)
>Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770
>
>Do others in Histology follow these CAP Guidelines for cleaning
>glassware?   I'm not sure if this question actually applies to Histology.
> I have never had an inspector ask me about it, but I'm wondering if
>anyone has been questioned.
>
>Laurie Colbert, HT (ASCP)
>Histology Supervisor
>PATH MD
>8158 Beverly Blvd.
>Los Angeles, CA  90048
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Re: [Histonet] Protocol for decalcifying and processing large bone section.

[Barry Rittman]

I would recommend using Kristensen's "sodium formate/formic acid" mixture.
This is more gentle than formic acid alone and almost as rapid.
Barry


On Tue, Aug 5, 2014 at 12:01 PM, DiCarlo, Margaret <
mdica...@kaleidahealth.org> wrote:

> I use 10% formic acid for large human bones but I don't think your bone
> sections are that large.
>
> Peggy DiCarlo
> Ortho Bone Lab
> BGMC
> 100 High St.
> Buffalo, NY  14203
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clough, Bret
> Sent: Tuesday, August 05, 2014 11:55
> To: Histonet list serv.
> Subject: [Histonet] Protocol for decalcifying and processing large bone
> section.
>
> Hi everyone,
>  I was hoping someone on histonet would being willing to help me by either
> sharing their protocol or advising me on decalcifying and processing large
> bone sections. The bone sections are from the femural head of sheep
> measuring roughly 1cm x 1 1/2 cm cube.  Currently I've been decalcifying
> the samples in 0.5M EDTA which is taking along time to decalcify this being
> the 19th day.  Any advise would be greatly appreciated!
>
>
> Sincerely,
>Bret Clough
>Texas A&M Health Science Center
>Temple, TX.
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RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770

[Jamal]

I am also the same as Joelle & Brian, It never been questioned by CAP
inspectors but since it's in the common checklist we have to follow that’s
why we have a policy with simple procedure and record, the procedure almost
the same as Brian procedure but some time we use the digital PH meter.


Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joelle
Weaver
Sent: Tuesday, August 05, 2014 10:33 PM
To: Cooper, Brian; Laurie Colbert; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770

Do the same as Brian. It is not that involved really, and its not worth
getting a deficiency over.  


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
> From: bcoo...@chla.usc.edu
> To: lcolb...@pathmdlabs.com; histonet@lists.utsouthwestern.edu
> Date: Tue, 5 Aug 2014 19:07:05 +
> CC: 
> Subject: [Histonet] RE: Glassware Cleaning, CAP Checklist Item GEN.41770
> 
> I've never been asked by a CAP auditor either, but our internal inspectors
used to inquire about this in a previous lab all the time.  We use
Bromocresol Purple as an indicator here, and we have a simple log sheet
wherein our crew just tests one piece of cleaned glassware each day.  They
write the type of glassware they test, and place a checkmark in a "yellow"
or "purple" column based upon what they see.  If "purple," they rewash,
retest and document accordingly. 
> 
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
> Department of Pathology and Laboratory Medicine
> Children's Hospital Los Angeles 
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
> Ph: 323.361.3357 Pager: 213-209-0184
> bcoo...@chla.usc.edu 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie
Colbert
> Sent: Tuesday, August 05, 2014 11:06 AM
> To: Histonet Post (histonet@lists.utsouthwestern.edu)
> Subject: [Histonet] Glassware Cleaning, CAP Checklist Item GEN.41770
> 
> Do others in Histology follow these CAP Guidelines for cleaning glassware?
I'm not sure if this question actually applies to Histology.  I have never
had an inspector ask me about it, but I'm wondering if anyone has been
questioned.
> 
> Laurie Colbert, HT (ASCP)
> Histology Supervisor
> PATH MD
> 8158 Beverly Blvd.
> Los Angeles, CA  90048
> (323) 648-3214 direct
> (424) 245-7284 main lab
> 
> The information contained in this transmission may contain privileged and
confidential information, including patient information protected by federal
and state privacy laws. It is intended only for the use of the person(s)
named above. If you are not the intended recipient, you are hereby notified
that any review, dissemination, distribution, or duplication of this
communication is strictly prohibited. If you are not the intended recipient,
please contact the sender by reply email and destroy all copies of the
original message.
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