RE: [Histonet] whole mount immunofluorescence staining for mouse aorta

[Elizabeth Chlipala]

Mark

Not sure why they would want to do this for IF staining.  The only thing I can 
think of that is similar to this is in mouse models of atherosclerosis we have 
run "en Face" analysis of the mouse aorta.  The entire mouse aorta is dissected 
out, trimmed of fat and cut open via a specific procedure, the entire aorta is 
then stained with Sudan IV to demonstrate the plaques and then pinned on a tar 
plate, images are captured for image analysis.It is also common in the 
studies to run aortic root analysis - we have done this on frozen sections with 
brightfield IHC.  We have experience in all of these aspects of tissue 
collection and preparation, some of the techniques may apply to what they are 
trying to do.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Elliott
Sent: Tuesday, August 19, 2014 3:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] whole mount immunofluorescence staining for mouse aorta

A colleague of mine has asked if I have any experience wholemount 
immunofluorescence staining for mouse aorta.  I don't have any experience with 
this so thought I would ask the group for any suggestions/protocols/ tips etc.
Thanks 
Mark

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[Histonet] whole mount immunofluorescence staining for mouse aorta

[Mark Elliott]

A colleague of mine has asked if I have any experience wholemount 
immunofluorescence staining for mouse aorta.  I don't have any experience with 
this so thought I would ask the group for any suggestions/protocols/ tips etc.
Thanks 
Mark
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[Histonet] IHC on free floating slices of brain

[Walter Benton]

Does anyone have information they could share on performing IHC on whole mount, 
sledge microtome free floating slices of brain? A Neuropathologist colleague is 
interested in doing this.

Thanks in advance for your help.

Walter Benton HT(ASCP)QIHC
Histology Supervisor
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.


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[Histonet] OCT Tissue fall out and reembedding

[Lewis, Patrick]

Hi everyone.


If you have several OCT blocks in which the Tissue has fallen out and to want 
to salvage them for IHC.  What is the best way to do so to minimize freeze-thaw 
damage.

These samples were harvested and then were probably snap frozen in Isopentane 
surrounded by dry ice and stored at -80 when they were made.

The people using these blocks now have had several of the blocks in which the 
tissue has popped out of the block.

We are going to get these blocks for IHC.

What is the best way to salvage these tissues so that I can do IHC on them.

Patrick.


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Re: [Histonet] bone saw for cutting slabs

[Sean McBride]

Hi Merissa,

Exakt technologies makes a wonderful saw designed specifically for exactly what 
you are trying to do with a hack saw.  It is a bit pricy though.  Contact Linda 
Durbin at 405-848-5800 for a quote.

Alternatively, you can use a wet saw designed for cutting stained glass.  Check 
out the one by Gryphon:  Not too expensive & cuts bone well if you take your 
time.  Other vendors like Mar-Med make alternative blades for this saw as well.


Good luck,


~Sean McBride


Scientific Specialist
Bone Tissue Engineering Center
Carnegie Mellon Research Institute
Suite 4311
700 Technology Drive
Pittsburgh, PA 15219-3124

412-268-8275 (o)
571-989-BONE (m)
412-268-8275 (fax)
smcbr...@andrew.cmu.edu 







On Aug 19, 2014, at 12:45 PM, M.O. wrote:

> Histoland! Happy Tuesday!
> 
> I just wanted to get your feedback on cutting slabs from human femora
> for histopathological analysis.
> 
> At them moment we are just using a hack saw to cut 7mm slabs from
> femora.  We notice some marks on the cartilage from sawing, so when we cut
> the tissue down after decalcification for histological preparation, we cut
> the thickness down to 4mm and remove the "damaged" tissue.
> 
> Would using some sort of bone saw damage the tissue even more or would it
> be comparable to using a hack saw?  Is there a saw that you recommend that
> is precise and easy to handle that doesn't damage tissue greatly?
> 
> Thank you so much for your help!
> 
> Sincerely,
> Merissa
> ___
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RE: [Histonet] bone saw for cutting slabs

[Barbara Tibbs]

Most hospitals that I've worked at that needed to cut bone used a Stryker bone 
saw.  The pathologists never mentioned damaged cartilage.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of M.O. 

Sent: Tuesday, August 19, 2014 3:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] bone saw for cutting slabs

Histoland! Happy Tuesday!

 I just wanted to get your feedback on cutting slabs from human femora
for histopathological analysis.

 At them moment we are just using a hack saw to cut 7mm slabs from
femora.  We notice some marks on the cartilage from sawing, so when we cut
the tissue down after decalcification for histological preparation, we cut
the thickness down to 4mm and remove the "damaged" tissue.

Would using some sort of bone saw damage the tissue even more or would it
be comparable to using a hack saw?  Is there a saw that you recommend that
is precise and easy to handle that doesn't damage tissue greatly?

Thank you so much for your help!

Sincerely,
Merissa
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[Histonet] tissue falling off slides

[Burnett, Brandy]

I forgot to mention that we are using a 3 in one HIER for target retrieval, 
instead of running

down (xylene-ETOH). Could this also contribute to tissue falling off?




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[Histonet] bone saw for cutting slabs

[M.O.]

Histoland! Happy Tuesday!

 I just wanted to get your feedback on cutting slabs from human femora
for histopathological analysis.

 At them moment we are just using a hack saw to cut 7mm slabs from
femora.  We notice some marks on the cartilage from sawing, so when we cut
the tissue down after decalcification for histological preparation, we cut
the thickness down to 4mm and remove the "damaged" tissue.

Would using some sort of bone saw damage the tissue even more or would it
be comparable to using a hack saw?  Is there a saw that you recommend that
is precise and easy to handle that doesn't damage tissue greatly?

Thank you so much for your help!

Sincerely,
Merissa
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[Histonet] n

[Stewart, Charles]

Respectfully,

Charles Stewart
Field Support Specialist
Leica Biosystems
412-737-7380
charles.stew...@leicabiosystems.com
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[Histonet] RE: Is there a Law for refusal of pathology services.

[Podawiltz, Thomas]

I would start with CAP/CLIA for their guidelines. Then check to see what the 
state regulations would be. 


Tom 


Tom Podawiltz HT (ASCP)
Histology Section Head 
LRGHealthcare
Laconia, NH 03246
 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Tuesday, August 19, 2014 10:45 AM
To: Histonet
Subject: [Histonet] RE: Is there a Law for refusal of pathology services.

I believe the question was asking for "laws". 

A law is different than what insurance companies may or may not prefer. They 
have 'rules' but have to follow 'laws'. Laws that might concern this are also 
enacted at both the national and state levels. Any state might differ over 
another.  All I can offer beyond that is, if really concerned, and it sounds 
like you are, take up the question with your Risk Management folks, or whatever 
department deals with that kind of stuff. 

This case is one you are aware of. It might shock you to learn how many times 
patients do not have their tissues examined, especially in clinics. The best 
record might the op-notes, as far as the insurance companies are concerned. 

To assume the patient won't be covered, that she has anterior motive, or that 
the lab is the sole documentation of a procedure is to make legal assumptions 
and, frankly, that's not a good idea. 

All of that said - it is, in my mind, prudent to get a diagnosis, but prudence 
is different than necessity and patient rights are pretty broad and are rarely 
narrowly interpreted. 

Thanks for reading this far down -

Bill (Not A Lawyer) O'Donnell

 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Tuesday, August 19, 2014 8:52 AM
To: Sue; Paula Pierce
Cc: Histonet
Subject: [Histonet] RE: Is there a Law for refusal of pathology services.

Also, legally speaking, if it isn't documented, it didn't happen in the eyes of 
the law.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Sue 

Sent: Monday, August 18, 2014 9:43 PM
To: Paula Pierce
Cc: Histonet
Subject: Re: [Histonet] Is there a Law for refusal of pathology services.

I agree, if you do not document that a specimen was removed most likely 
insurance will deny the clain.

SPaturzo
TJU
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[Histonet] Georgia Society for Histotechnology

[Zimmerman, Billie]

The Georgia Society for Histotechnology is planning for next year's 
HISTOPALOOZA!
HISTOPALOOZA
APRIL 17 - 19, 2015
LEGACY LODGE AT LAKE LANIER ISLANDS

This is a call for abstracts for workshop speakers.  We will once again offer 
the HT/HTL review, the QIHC review, and the microscopic anatomy by Ely Klar.  
The GSH offers free membership and we encourage you to join NSH with is  $$ 
savings.
Take a look at our website and contact any BOD member for information about 
what the GSH can offer you.  Our next newsletter will be on our website by 
September 6th, with updates on HISTOPALOOZA! 2015

Here's the link for that awesome place at Lake Lanier.  It looks like a ski 
resort and it's hard to believe you're in Atlanta, to be honest.
http://www.lakelanierislands.com/meetings?rt=google|cpc|LAL03-Lanier-Islands-Meetings-Brand|legacy%20lodge%20and%20conference%20center&gclid=CKvy7IfOn8ACFc1i7AodfFcARQ

Hope I see many of you in Austin!

Warm Regards,
Wanda K Simons HT, ASCP
GSH President
ws/bz





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[Histonet] Is there a Law for refusal of pathology services.

[White, Lisa M.]

I would think the patient would have to sign an Against Medical Advise
release just as if they were leaving the ER even though you know they
were having a heart attack. 

 

Should the reason for refusal to have the specimen processed is they do
not have insurance and you do a gross only then they will still be
charged for a gross only specimen.

 

Lisa White, HT(ASCP)

Classification: Internal and External Use\\Not VA Sensitive
This message has been categorized by White, Lisa M. on Tuesday, August
19, 2014 at 11:06:35 AM in accordance with VA Handbook 6500


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[Histonet] RE: Is there a Law for refusal of pathology services.

[O'Donnell, Bill]

I believe the question was asking for "laws". 

A law is different than what insurance companies may or may not prefer. They 
have 'rules' but have to follow 'laws'. Laws that might concern this are also 
enacted at both the national and state levels. Any state might differ over 
another.  All I can offer beyond that is, if really concerned, and it sounds 
like you are, take up the question with your Risk Management folks, or whatever 
department deals with that kind of stuff. 

This case is one you are aware of. It might shock you to learn how many times 
patients do not have their tissues examined, especially in clinics. The best 
record might the op-notes, as far as the insurance companies are concerned. 

To assume the patient won't be covered, that she has anterior motive, or that 
the lab is the sole documentation of a procedure is to make legal assumptions 
and, frankly, that's not a good idea. 

All of that said - it is, in my mind, prudent to get a diagnosis, but prudence 
is different than necessity and patient rights are pretty broad and are rarely 
narrowly interpreted. 

Thanks for reading this far down -

Bill (Not A Lawyer) O'Donnell

 



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara Tibbs
Sent: Tuesday, August 19, 2014 8:52 AM
To: Sue; Paula Pierce
Cc: Histonet
Subject: [Histonet] RE: Is there a Law for refusal of pathology services.

Also, legally speaking, if it isn't documented, it didn't happen in the eyes of 
the law.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Sue 

Sent: Monday, August 18, 2014 9:43 PM
To: Paula Pierce
Cc: Histonet
Subject: Re: [Histonet] Is there a Law for refusal of pathology services.

I agree, if you do not document that a specimen was removed most likely 
insurance will deny the clain.

SPaturzo
TJU
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[Histonet] RE: Tissue falling off

[Burnett, Brandy]

Teri~
Thanks so much for the very useful feedback...
We have only encountered this problem recently and
these suggestions will be very useful!

~Brandy


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Terri  Braud 
[tbr...@holyredeemer.com]
Sent: Friday, August 15, 2014 1:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Tissue falling off

Hi - Just a couple of suggestions -
I've seen this periodically crop up during the years.  There are a
couple of things to consider:
Water baths are completely clean - after dumpling water and removing
paraffin debris with whatever you use - wipe out the bath with clean
absolute alcohol.
Be careful where you spray - make sure that if you use anything like
Paraguard, or similar stuff that keeps paraffin from sticking, that it
is used sparingly, or preferably, not at all.  We had a situation where
we had a trial sample that someone was using to clean her microtome and
forceps and the stuff got everywhere and tissues started falling off.
Your water in the waterbaths is clean - Fresh DI water.  Once we had a
contaminant in our DI water, and the patient tissues slid right off our
slides.  We use clean slides, good quality water and super clean
waterbaths.
Is your heater up and running? - Our slides are stained on a stainer
with built in slide dryers.  We used to have a problem with the first
rack of patient slides' tissue falling off because the slide dryer was
not hot enough for our short dry cycle.  We now send a rack through
first, which turns on the heaters, then load the patient rack.
Hope this gives you a few ideas
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

   7. tissue falling off slides (Burnett, Brandy)
From: "Burnett, Brandy" 
Subject: [Histonet] tissue falling off slides

Our lab has encountered an issue with tissue falling off of the slides.
It is mostly the patient tissue
that is falling off. Some of the Techs are using control slides that
have been pre-cut and I am wondering
if this might be part of the problem. Any information would be very much
appreciated.
Thanks Again,
Brandy Burnett, HTL


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RE: [Histonet] isopropanol for tissue processing

[Wheelock, Timothy R.]

Hi Julio:

I have been using isopropanol for as long as I can remember.
I work only with post-mortem brain tissue (in standard-sized cassettes) in a 
neuropathology laboratory at a brain tissue bank.
Because the brains have been previously fixed in  10% neutral buffered formalin 
for a month, I do not have to use formalin on the processor.
Anyway, I start out with 30% isopropanol, then 50%, 80%, 95%, 3 stations of 
100%,  3 stations of xylene, then into  paraffin.
I cannot remember whether we used isopropanol in surgical histology years ago, 
when I was at another hospital, but I think we did.
Good luck,

Tim

Tim Wheelock
Harvard Brain Tissue Resource Center
McLean Hospital
Belmont, MA, USA

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Julio Benavides
Sent: Tuesday, August 19, 2014 9:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] isopropanol for tissue processing

Hi,

In our histology lab, we are considering to move from ethanol to isopropanol 
for processing formalin fixed samples (post mortem samples, so big samples). I 
have been diving in the histonet archives and have found that the subject has 
been laready discussed, but somehow I cannot find a definitive message stating 
"Yes, we have tried and it works perfectly. This is our protocol".  So here I 
am, writing the forum to see if anyone can kindly confirm that the move from 
ethanol to isopropanol is not a traumatic one, and if any of you has eliminated 
the xylene as clearing agent as well (and what have you used instead. 
Isopropanol/paraffin at 50c or straight into paraffin?)

I have been speaking with Rene J Buesa who, besides a very helpful person, is 
highly enthusiast in the use of isopropanol. He has almost converted me to the 
isopropanol side!


Thank you very much for your time and help

Regards

Julio Benavides
Instituto de Ganadería de Montaña (CSIC-University of Leon) Spain



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[Histonet] RE: Is there a Law for refusal of pathology services.

[Barbara Tibbs]

Also, legally speaking, if it isn't documented, it didn't happen in the eyes of 
the law.

Barbara S. Tibbs
Histology Supervisor
Accurate Diagnostic Labs
South Plainfield, NJ
barbara.ti...@accuratediagnosticlabs.com
732-839-3374
Cell: 610-809-6508



From: histonet-boun...@lists.utsouthwestern.edu 
 on behalf of Sue 

Sent: Monday, August 18, 2014 9:43 PM
To: Paula Pierce
Cc: Histonet
Subject: Re: [Histonet] Is there a Law for refusal of pathology services.

I agree, if you do not document that a specimen was removed most likely 
insurance will deny the clain.

SPaturzo
TJU
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[Histonet] Exakt benchmarks

[Ronda Mire]

Hello Netters,

 

I am seeking information on Exakt technology benchmarks.  Those of you using
this technology, will you kindly share with me how many specimens/slides can
be completed in a work day?  I am new to this technology and have been
tasked with establishing benchmarks/goals for the lab techs.  Thanks

 

Ronda Mire

Laboratory Manager/Chief Technologist

CVPath Institute

19 Firstfield Rd

Gaithersburg, MD 20878

301-208-3570 x112

rm...@cvpath.org

 

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[Histonet] isopropanol for tissue processing

[Julio Benavides]

Hi,

In our histology lab, we are considering to move from ethanol to 
isopropanol for processing formalin fixed samples (post mortem samples, 
so big samples). I have been diving in the histonet archives and have 
found that the subject has been laready discussed, but somehow I cannot 
find a definitive message stating "Yes, we have tried and it works 
perfectly. This is our protocol".  So here I am, writing the forum to 
see if anyone can kindly confirm that the move from ethanol to 
isopropanol is not a traumatic one, and if any of you has eliminated the 
xylene as clearing agent as well (and what have you used instead. 
Isopropanol/paraffin at 50c or straight into paraffin?)


I have been speaking with Rene J Buesa who, besides a very helpful 
person, is highly enthusiast in the use of isopropanol. He has almost 
converted me to the isopropanol side!



Thank you very much for your time and help

Regards

Julio Benavides
Instituto de Ganadería de Montaña (CSIC-University of Leon)
Spain



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[Histonet] RE: Is there a Law for refusal of pathology services.

[Mark Turner]

Nicole,

Does the surgical consent form address the issue, including consent for further 
testing?  Many do, and this should cover the issue. She does, however, have the 
right to revoke any prior permissions at any time she decides to change her 
mind.  I would have her sign a statement that she is refusing to allow 
diagnosis and include it as part of a gross-only report.  Holding the tissue as 
documentation of the procedure is wise, and can be justified to the patient 
rather easily since giving the specimen back to the client  involves handing 
them a hazardous substance (formalin), something the OSHA folks frown upon.  

I'm also curious how the payors will view this. Sounds like a ready-made excuse 
for instance denial.  Keep us posted.

Mark Turner,  Ph.D., HT(ASCP)QIHC
Manager, Histology/IHC
 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nicole Tatum
Sent: Monday, August 18, 2014 5:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Is there a Law for refusal of pathology services.

Please help,



We had a patient today who had a punch bx of what is believed to be a clinical 
dermatofibroma.  The  patient stated they did not wish for the specimen to be 
sent for clinical testing. Our ARNP discussed the need for pathology at length 
and the patient stated she was a nurse and could sign a waiver denying 
pathology services.  I have googled and gooled trying to find any specific law 
or statue. I can only find information pertaining to research or donated 
tissue. Stating a person no longer has rights "or ownership"to the tissue once 
consented and removed, but this case is not for research.  Could someone pls 
share an actual law with me. Seems silly to consent to the surgery but not to 
the diagnosis. Im not sure what to do at this point. Have them sign a document 
on our company letterhead stating there denial of services? Hold the tissue 
hold long? Accession it but only do gross description? Charge anything? Any 
thoughts or imformation would be greatly appreciated.



Nicole Tatum HT BSH
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Re: [Histonet] Is there a Law for refusal of pathology services.

[Emily Brown]

What is the reasoning for this?? I think the insurance company would want a
diagnosis! Does this person enjoy surgery for the fun of it and not
actually need it? Or maybe she has another problem she doesn't want her
insurance to know about? Although if she has insurance she can't be
retroactively declined, can she?
How odd.

Emily
On Aug 18, 2014 5:04 PM, "Nicole Tatum"  wrote:

> Please help,
>
>
>
> We had a patient today who had a punch bx of what is believed to be a
> clinical dermatofibroma.  The  patient stated they did not wish for the
> specimen to be sent for clinical testing. Our ARNP discussed the need for
> pathology at length and the patient stated she was a nurse and could sign a
> waiver denying pathology services.  I have googled and gooled trying to
> find any specific law or statue. I can only find information pertaining to
> research or donated tissue. Stating a person no longer has rights "or
> ownership"to the tissue once consented and removed, but this case is not
> for research.  Could someone pls share an actual law with me. Seems silly
> to consent to the surgery but not to the diagnosis. Im not sure what to do
> at this point. Have them sign a document on our company letterhead stating
> there denial of services? Hold the tissue hold long? Accession it but only
> do gross description? Charge anything? Any thoughts or imformation would be
> greatly appreciated.
>
>
>
> Nicole Tatum HT BSH
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> Histonet mailing list
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>
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