[Histonet] Mohs

2014-09-23 Thread michele carr 
Hello I perform mohs cutting a few days a week and I noticed that on fatty
skin tissue I don't get the interior of the skin section. I only seem to
get the epidermis. Is there a trick to getting the fatty tissue too. I
tried bumping up the microns but that only helped a bit.
Thanks for your responses.

Michele Carr
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Re: [Histonet] RE: Artifact in IHC

2014-09-23 Thread Donna Maney 
Geoff and Terry,
Thanks so much for your input. All of the Histonet posts went into my spam so I 
just saw these today, but in the meantime by coincidence we ended up following 
your suggestions. To us, it looked like the tissue was drying out and cracking 
before being coverslipped. A new technician was being trained, and was perhaps 
trying too hard to drain all of the xylene off the slide. She noted that the 
longer she took to coverslip the slide, the worse the artifact was. So we 
soaked off the coverslips, rehydrated down through 70% and into 50% then water, 
then dehydrated and coverslipped again. I'd say the artifact is 95% gone. 

So, I don't know if it was just being speedier with the coverslip or the lower 
concentrations of alcohol and water soak that did the trick. But we will be 
implementing both now.

Thanks again,
Donna

On Tue, 9/23/14, Geoff  wrote:

 Subject: Re: [Histonet] RE: Artifact in IHC
 To: histonet@lists.utsouthwestern.edu
 Date: Tuesday, September 23, 2014, 3:40 AM
 
 Teri makes an excellent
 point about higher alcohols causing 
 precipitation of phosphate salts.
 
 Geoff
 
 On 9/22/2014 1:17 PM, Teri Johnson wrote:
 > Hi Donna,
 >
 > Interesting conundrum! A few questions:
 >   - Do you see it in tissues
 that have not undergone any staining, just floated in buffer
 and coverslipped?
 >   - Have
 you tried wet mounting them after staining and prior to
 dehydration and clearing to see if the artifact is there?
 >   - Have you recently changed
 glove manufacturers, could this be talc or some other powder
 from gloves?
 >   - Are you
 dehydrating out of PBS into an alcohol concentration higher
 than 70%? You could be getting phosphate precipitation. Try
 using a water rinse prior to alcohol or make sure your first
 alcohol concentration is 70% or lower.
 >
 > Those are the weird
 things that came to mind. I hope this helps,
 >
 > Teri Johnson
 > Manager Clinical Trial Testing
 > Genoptix, Inc.
 >
 Carlsbad, CA 92008
 >
 >
 >
 >
 
 >
 > CONFIDENTIALITY NOTICE: This e-mail
 message, including any attachments, is for the sole use of
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 or its subsidiaries. Any unauthorized review, use,
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 -- 
 --
 **
 Geoff McAuliffe, Ph.D.
 Neuroscience and Cell Biology
 Robert Wood Johnson Medical School
 675 Hoes Lane, Piscataway, NJ 08854
 voice: (732) 235-4583; fax: -4029
 mcaul...@rwjms.rutgers.edu
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[Histonet] RE: Surgical Pathology reports

2014-09-23 Thread Horn, Hazel V 
Debby there is critical test results to call.  Unexpected findings such as 
malignant when benign were expected for example.   Our pathologists call and it 
is documented in the pathology report when they called and who they spoke to.   
If the clinicians are waiting on a diagnosis and results are delayed for 
special testing the pathologists will call and tell them what is going on with 
their case.  There is a CAP standard for this very issue, what you do about 
unexpected findings.

Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hor...@archildrens.org
archildrens.org






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie
Sent: Tuesday, September 23, 2014 12:23 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Surgical Pathology reports

Do pathologists ever call pathology reports to a physician?  If so, what types 
of diagnoses do they call?  There are no "critical" test results in Pathology 
that CAP deems necessary to call as in other areas of the laboratory..  How do 
others handle this?

Debra Lake  MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN  46952
(765) 660-6521
Fax: (765-651-7330)






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RE: [Histonet] RE: Surgical Pathology reports

2014-09-23 Thread Joelle Weaver 
Pretty much " same here". The policy I wrote complies with the ANP checklist 
for " critical findings", give fairly broad scope the pathologist to determine 
when this is needed or just good patient care, and provides for documentation 
when this occurs. 


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
> From: toni.rathbo...@rwjuh.edu
> To: debbie.l...@mgh.net; histonet@lists.utsouthwestern.edu
> Date: Tue, 23 Sep 2014 17:34:16 +
> CC: 
> Subject: [Histonet] RE: Surgical Pathology reports
> 
> Yes, more often than you may imagine. Unexpected findings are always called 
> and documented. See ANP12175. Although not required, another reason is the 
> courtesy extended by the pathologist to the physician. This may occur if a 
> preliminary report is to be issued, or the final report not signed out while 
> the pathologist waits for additional tests to be completed. The pathologist 
> may call if he/she knows that the physician is especially interested in 
> getting the results for patient care reasons, and wants to explain why there 
> may be delays to the final report.
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie
> Sent: Tuesday, September 23, 2014 1:23 PM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: [Histonet] Surgical Pathology reports
> 
> Do pathologists ever call pathology reports to a physician?  If so, what 
> types of diagnoses do they call?  There are no "critical" test results in 
> Pathology that CAP deems necessary to call as in other areas of the 
> laboratory..  How do others handle this?
> 
> 
> Debra Lake  MT(ASCP)
> Manager Micro, Blood Bank, Pathology
> Marion General Hospital
> Marion, IN  46952
> (765) 660-6521
> Fax: (765-651-7330)
> 
> 
> 
> 
> 
> 
> If you are not the intended recipient(s), you are notified that any 
> disclosure, copying, distribution or any action taken or omitted to be taken 
> in reliance on the contents of this information is prohibited and may be 
> unlawful. If you receive this message in error, or are not the named 
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[Histonet] RE: Surgical Pathology reports

2014-09-23 Thread Rathborne, Toni 
Yes, more often than you may imagine. Unexpected findings are always called and 
documented. See ANP12175. Although not required, another reason is the courtesy 
extended by the pathologist to the physician. This may occur if a preliminary 
report is to be issued, or the final report not signed out while the 
pathologist waits for additional tests to be completed. The pathologist may 
call if he/she knows that the physician is especially interested in getting the 
results for patient care reasons, and wants to explain why there may be delays 
to the final report.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lake,Debbie
Sent: Tuesday, September 23, 2014 1:23 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Surgical Pathology reports

Do pathologists ever call pathology reports to a physician?  If so, what types 
of diagnoses do they call?  There are no "critical" test results in Pathology 
that CAP deems necessary to call as in other areas of the laboratory..  How do 
others handle this?


Debra Lake  MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN  46952
(765) 660-6521
Fax: (765-651-7330)






If you are not the intended recipient(s), you are notified that any disclosure, 
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[Histonet] Surgical Pathology reports

2014-09-23 Thread Lake,Debbie 
Do pathologists ever call pathology reports to a physician?  If so, what types 
of diagnoses do they call?  There are no "critical" test results in Pathology 
that CAP deems necessary to call as in other areas of the laboratory..  How do 
others handle this?

Debra Lake  MT(ASCP)
Manager Micro, Blood Bank, Pathology
Marion General Hospital
Marion, IN  46952
(765) 660-6521
Fax: (765-651-7330)






If you are not the intended recipient(s), you are notified that any disclosure, 
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[Histonet] At long last, a Murine CD4 monoclonal antibody that works on FFPE tissues

2014-09-23 Thread gayle callis 
Dear Histonetters, 

 

This antibody is going to be very welcome for those working with NBF fixed
paraffin embedded murine tissue.It is a rat antiMouse CD4  for FFPE
tissue.   For those interested go to Affymetrix/eBioscience, Rat antiMouse
CD4, clone 4SM95 (L3T4/Ly-4,  Catalog number 14-9766.   It also comes
conjugated to the company's eFluor 570, equivalent to Alexa 555 although one
could do their own conjugation to their favorite Alexa or DyLight dye.   

 

Now all we need is a mouse CD8 that works on FFPE for total happiness since
we often do these two antibodies in tandem as a double IF stain or on
adjacent sections for enzyme IHC.

 

Take care

 

Gayle M. Callis

HTL/HT/MT(ASCP)  

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[Histonet] Direct Hire/Permanent Histotech Job in Minnesota

2014-09-23 Thread Melissa Owens (Phelan) 
Good Morning,

I have a permanent/direct hire position available for a Histotech in
Rochester, MN area. Please contact me for the details if you are interested.
Thank you!

Melissa Owens (Phelan)
President, Laboratory Staffing
Allied Search Partners
www.linkedin.com/in/melissaphelan/

http://www.alliedsearchpartners.com 

T: 888.388.7571 ext. 102

F: 888.388.7572






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[Histonet] JCAHO Competency Requirements

2014-09-23 Thread Jones, Laura 
Good Morning!  We are switching from CAP to JCAHO for our inspection and 
accreditation.  I'm reading a lot about "Six Method Competency", but most of 
the information I have is geared toward blood testing.  If anyone out there can 
offer any suggestions as to how I should reformat our competency forms to 
satisfy JCAHO, I would appreciate any help.  Thanks in advance!


Laura Jones B.A., HT, PBT (ASCP) | Lead Tech, Histology | Community Health 
Systems
740 East State Street | Sharon PA | Phone (724)983-3950 | Fax (724)983-3982
www.sharonregional.com | 
lpjo...@srhs-pa.org



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