[Histonet] myogenin control tissue

[drmoses111]

Does anyone have a control block for myogenin? My only block of 
rhabdomyosarcoma is nearly exhausted. Please contact me at 
drmoses...@comcast.net,, 
Thank you, 
Renee Boston-Moses HT,QIHC 
Virtua Health System 
Histology Department 
100 Bowman Dr. 
Voorhees, NJ 08043 
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[Histonet] Pathologist Assistant in Newport Beach, CA

[Yang, Mari]

Dear Histonet,



I am pleased to announce a Pathologist Assistant position for Hoag
Hospital, located in Newport Beach, CA is now available. All candidates
PA(ASCP) certified or eligible are encouraged to apply. For further
information, please visit http://www.hoag.org/Pages/Hoag-Careers.aspx
and search pathologist assistant.



Regards,

Mari



Mari Yang, MHA, CTCM(ASCP)HTLCM

Manager | Anatomic Pathology & Cytology

Hoag Memorial Presbyterian Hospital

One Hoag Drive, PO Box 6100, Newport Beach, CA 92658

949.764.5631 | mari.y...@hoag.org



P Save a tree, please don't print this e-mail unless you really need to.



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Thank you,

Hoag Memorial Hospital Presbyterian and its Affiliates

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Re: [Histonet] Glacial Acetic Acid subs.

[Jennifer MacDonald]

vinegar.  you can buy "cleaning" vinegar.  It is acetic acid, but not 
concentrated.



From:   "Armstrong, Karoleigh T" 
To: "histonet@lists.utsouthwestern.edu" 

Date:   09/30/2014 11:09 AM
Subject:[Histonet] Glacial Acetic Acid subs.
Sent by:histonet-boun...@lists.utsouthwestern.edu



Does any one know of a substitute for Glacial Acetic Acid? We use it to 
clean the lines on the VIP and in the Eosin. Our Corp. heads want to 
eliminate Glacial in concentration form from all its hospitails.
Thanks,
Karoleigh Armstrong, HT (ASCP)


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Re: [Histonet] microtome stage

[Alan Bright]

Donna, I would take the powder coating off with a paint stripper, try 
sectioning, if happy have the holder electro plated which will be a conductive 
coating that will protect the base metal from corrosion. Cost under $20.
BR Alan Bright, BIC...Cambs..UK

Sent from my iPhone

> On 30 Sep 2014, at 02:53, Donna Maney  wrote:
> 
> I have a Leica SM2000 microtome, circa 2004, that has recently developed an 
> issue with the dry ice tray. Up until this year, we've never had any problems 
> with the specimen adhering to the platform. But suddenly we cannot cut 
> specimens because they easily become dislodged. We use 30% sucrose to stick 
> the specimen to the dry ice tray. When we borrow a dry ice tray from another 
> lab, the problem goes away.
> 
> Our dry ice tray is powder coated and we can see the the coating is rubbing 
> away, but it does not seem to be coming off with the specimen. Rotating the 
> tray so that the specimen is attached at a different location on the platform 
> does not help. We have cleaned the dry ice tray with hot soapy water, rinsed 
> with distilled water and then with ethanol. 
> 
> Leica told me that the new trays do not have this powder coating, which makes 
> me think they know about a problem, but the person I spoke to on the phone 
> was not aware of one.
> 
> I'd be glad to hear from anyone who has experienced the same thing or who has 
> suggestions. A new tray will cost $500-$1000 so I'd like to troubleshoot with 
> this one to the extent that it is appropriate.
> 
> Thanks,
> Donna
> 
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> 
> -- 
> 

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[Histonet] Glacial Acetic Acid subs.

[Armstrong, Karoleigh T]

Does any one know of a substitute for Glacial Acetic Acid? We use it to clean 
the lines on the VIP and in the Eosin. Our Corp. heads want to eliminate 
Glacial in concentration form from all its hospitails.
Thanks,
Karoleigh Armstrong, HT (ASCP)


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only for the use of the individual(s) and entity named in the message. If you 
are not an intended recipient of this message, please notify the sender 
immediately and delete the material from your computer. Do not deliver, 
distribute or copy this message and do not disclose its contents or take any 
action in reliance on the information it contains.
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[Histonet] RELIA Special Histology Job Alert 9-30-2014. First Look at Some of the Best Opportunities this Fall!!

[Pam Barker]

Hi Histonetters!!
How are you doing today? 
Are you enjoying a fun filled Fall?  
For me the Summer just flew by!!  
I have been so busy here at RELIA.   
I have made a lot of histotechs happy with the positions
we landed for them this summer!!
I am sending this special alert out because I am starting the Fall just like
I started the Summer: 
WITH SOME OF THE BEST JOB OPPORTUNITIES OUT THERE!!!

I am especially excited about these openings because they are with several
of my best clients.  Why do I refer to them as best clients . Well for a
variety of reasons:  
Location, 
The rest of the team,
The fantastic manager, 
Excellent benefits, 
Generous relocation 
And most importantly I like a client that only has openings due to growth. 

Here is a list of the positions that I am most excited about:
Seattle, WA - Director of Safety Assessment
Hammond, IN - AP Manager
Hammond, IN - Histotechnician
Austin, TX - Histotechnician
Austin, TX - IHC Specialist
Fayetteville, AR - Dermpath Histotech 
Norfolk, VA - Histotechnologist - 15K Sign On Bonus!
Philadelphia, PA - Histotechnician
Lafayette, LA - Cytotech
Wilmington, NC - Cytotech

If you or anyone you know is interested in more details   I can be reached
toll free at 866-607-3542 or rel...@earthlink.net. 
If you are starting to save up your Christmas money remember I pay a
referral fee to you if I place someone that you refer to me.  

Have a Great Day!!

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





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[Histonet] From: Stephanie Moore

[Stephanie Moore]

Hi histonet



http://buymobile.kz/tail.php?cool=qzmvknxxxw3181cnp


Stephanie Moore
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RE: [Histonet] RE: Negative Control for Validation

[Sebree Linda A]

Jamal,

We use the same patient cases throughout the validation procedure.  So each 
block gets stained with the new antibody (optimized protocol already signed off 
by IHC director or designee), vimentin (+ reagent control) and negative control 
serum (- reagent control).  As stated earlier, the negative tissue control is 
any non-staining negative tissue elements, expected to be negative, within the 
block.  

If you're interested I can send you our Validation Protocol Proposal form under 
separate cover.

Linda

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-Original Message-
From: Jamal [mailto:j.rowa...@alborglaboratories.com] 
Sent: Tuesday, September 30, 2014 5:15 AM
To: Sebree Linda A; 'Arrington, Karla A'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Negative Control for Validation

Hi Linda
can you give us more details about the controls selection and processing 
procedures.



Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832| j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Tuesday, September 30, 2014 12:43 AM
To: 'Arrington, Karla A'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Negative Control for Validation

Hey Karla,

We use Vimentin as a positive reagent control, our negative control serum for 
the negative reagent control and for the negative tissue control, the 
pathologist reviews the antibody stained slide and OK's the internal negative 
tissue elements as appropriately negative.

Hope that helps,

Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arrington, 
Karla A
Sent: Monday, September 29, 2014 1:25 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Negative Control for Validation

We are beginning the process of validating new antibodies using the BenchMark 
XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately positive, 
is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately negative, 
what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per antibody, 
or per case that will be used for validation?

Thanks All!
Karla

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarring...@anthc.org

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RE: [Histonet] RE: Negative Control for Validation

[Jamal]

Hi Linda
can you give us more details about the controls selection and processing
procedures.



Best Regards,


Jamal M. Al Rowaihi Anatomic Pathology Supervisor   | Al Borg
Medical Laboratories |  Mobile +966 503629832|
j.rowa...@alborglaboratories.com 
Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
www.alborglaboratories.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A
Sent: Tuesday, September 30, 2014 12:43 AM
To: 'Arrington, Karla A'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Negative Control for Validation

Hey Karla,

We use Vimentin as a positive reagent control, our negative control serum
for the negative reagent control and for the negative tissue control, the
pathologist reviews the antibody stained slide and OK's the internal
negative tissue elements as appropriately negative.

Hope that helps,

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arrington,
Karla A
Sent: Monday, September 29, 2014 1:25 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Negative Control for Validation

We are beginning the process of validating new antibodies using the
BenchMark XT but have some questions for validation.

Question:  For a positive reagent control that stains appropriately
positive, is a known control block used? If not, what is used?
Question:  For a negative reagent control that stains appropriately
negative, what is ran?
Question:  Is a negative tissue slide ran for all of our 10 cases per
antibody, or per case that will be used for validation?

Thanks All!
Karla

Karla Arrington, HT(ASCP), HIT(AHIMA)
Lead Histology Technician
ANMC Pathology
907-729-1810
kaarring...@anthc.org

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