RE: [Histonet] RE: white plastic scrapers

[Joelle Weaver]

I believe at some point I got some at a paint shop like Sherwin Williams. Carry 
mine with me in my "box of tools", works great.


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
> From: algra...@email.arizona.edu
> To: bmolin...@texasheart.org; Histonet@lists.utsouthwestern.edu
> Date: Tue, 14 Oct 2014 16:22:43 +
> CC: 
> Subject: [Histonet] RE: white plastic scrapers
> 
> Southeast Pathology was giving out scrapers at NSH this year but in case you 
> didn't get one - go to the cooking section of Target or Walmart and see if 
> they have a scraper to use on teflon surfaces. A squeegee works good on 
> molten wax surfaces too. Otherwise, a snow scraper might work too.
> 
> Retired but lurking,
> Andi G.
> 
> 
> From: histonet-boun...@lists.utsouthwestern.edu 
> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Betsy Molinari 
> [bmolin...@texasheart.org]
> Sent: Tuesday, October 14, 2014 5:04 AM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] white plastic scrapers
> 
> Hi all,
> Where can I purchase the whit plastic scrapers used to scrape the paraffin 
> off embedding centers and other surfaces. I believe ours went out with the 
> trash.
> Thanks.
> 
> Betsy Molinari HT(ASCP)
> Texas Heart Institute
> Cardiovascular Pathology
> 6770 Bertner Ave
> Houston,TX 77030
> 832-355-6524 (lab)
> 832-355-6812 (fax)
> 
> 
> 
> 
> 
> 
> 
> Betsy Molinari
> Senior Histology Research Technician
> 832-355-6524 | bmolin...@texasheart.org | 
> www.texasheart.org
> 
> 
> 
> 6770 Bertner Ave., MC 1-283, Houston, TX 77030
> 
> 
> 
> [Texas Heart 
> Institute][THI
>  News] [THI on Facebook]   
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RE: [Histonet] RE: Labeling Errors

[Joelle Weaver]

Thanks Tim, these references are VERY helpful.


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
> From: timothy.mor...@ucsfmedctr.org
> To: lcolb...@pathmdlabs.com; histonet@lists.utsouthwestern.edu
> Date: Tue, 14 Oct 2014 17:57:46 +
> CC: 
> Subject: [Histonet] RE: Labeling Errors
> 
> Laurie, 
> 
> The question to ask is, what is ACHIEVABLE with the technology you have in 
> place? The term is ALRA or ALRP...As Low as Reasonably Achievable, or 
> Possible. 
> 
> For instance, when we hand-wrote slide labels, and used offline cassette 
> printers we accepted that we would have a rather high rate of human errors. 
> Same with hand-applying labels after staining.
> 
> With barcoding, printing cassettes directly from the LIS, printing and 
> applying labels at the microtome our error rate is down by an order 
> magnitude, but occasional errors still occur due to humans "shortcutting"  
> the system, or faults in the system itself (ie, not flagging duplicate labels 
> scanned). None of those errors are acceptable but we still need to figure out 
> how to design the system to prevent them. 
> 
> The papers below give numbers to this problem. The patient ID error without 
> automation came out to 4+ per 1000 specimens. 
> 
> The general error rate was/is:
> 1/100 with all hand-written workflow
> 1/1000 with LIS printing of cassettes and labels, but human-applied
> 1/10,000 with barcoding and single piece workflow throughout the system and 
> interfaces to instruments (stainers)
> 
> Makary MA et al. Surgical specimen identification errors  Surgery 2007 
> Apr;141(4):450-5 
> Nakhleh RE, et al. Amended reports ... Q-probes study of 1,667,547 
> accessioned cases ...  Arch Pathol Lab Med. 1998 Apr;122(4):303-9
> Resar RK. Making noncatastrophic health care processes more reliable...  
> Health Serv Res. 2006; 41:1677-1689.
> 
> 
> 
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> San Francisco, CA
> 
> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for 
> the sole use of the intended recipient(s) and may contain confidential, 
> proprietary, and/or privileged information protected by law. If you are not 
> the intended recipient, you may not use, copy, or distribute this email 
> message or its attachments. If you believe you have received this email 
> message in error, please contact the sender by reply email and destroy all 
> copies of the original message.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
> Sent: Tuesday, October 14, 2014 10:21 AM
> To: Histonet Post (histonet@lists.utsouthwestern.edu)
> Subject: [Histonet] Labeling Errors
> 
> Is there a national average or benchmark for "acceptable" labeling errors in 
> Histology?
> 
> Laurie Colbert HT (ASCP)
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[Histonet] RE: Slides from antibody validation

[Joe W. Walker, Jr.]

On a recent CAP inspection, we did have an inspector ask to see our slides from 
our HER2 validation.  For the past 5 years, we have kept all of the slides from 
our validation studies along with the paperwork in a notebook.  These glass 
slide holders have worked well for keeping everything together in one place for 
inspections: http://www.rochester100.com/store/p/195-SL-3-182.aspx

Interestingly enough, on a recent Joint Commission inspection last year, an 
inspector made a similar request to see an example of an IHC that we had 
validated.

Joe W. Walker, Jr. MS, SCT(ASCP)CM
Manager of Anatomical Pathology, Microbiology and Reference
Rutland Regional Medical Center
160 Allen Street, Rutland, VT 05701
P: 802.747.1790  F: 802.747.6525
Email joewal...@rrmc.orgwww.rrmc.org

Our Vision:
To be the Best Community Healthcare System in New England

Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet 
Recognition® and the Governor's Award for Performance Excellence


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell, Lynne
Sent: Tuesday, October 14, 2014 12:35 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Slides from antibody validation

Following antibody validation studies, do you keep your slides accessible for 
CAP to peruse during inspection?  Has anyone had CAP actually ask to see your 
slides?  I feel that keeping the validation forms, signed off by the medical 
director, is sufficient, but I would like other opinions.

As always, thanks for any and all guidance!

Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
130 Fisher Road
Berlin, VT  05641
802-371-4923



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[Histonet] RE: Labeling Errors

[Morken, Timothy]

Laurie, 

The question to ask is, what is ACHIEVABLE with the technology you have in 
place? The term is ALRA or ALRP...As Low as Reasonably Achievable, or Possible. 

For instance, when we hand-wrote slide labels, and used offline cassette 
printers we accepted that we would have a rather high rate of human errors. 
Same with hand-applying labels after staining.

With barcoding, printing cassettes directly from the LIS, printing and applying 
labels at the microtome our error rate is down by an order magnitude, but 
occasional errors still occur due to humans "shortcutting"  the system, or 
faults in the system itself (ie, not flagging duplicate labels scanned). None 
of those errors are acceptable but we still need to figure out how to design 
the system to prevent them. 

The papers below give numbers to this problem. The patient ID error without 
automation came out to 4+ per 1000 specimens. 

The general error rate was/is:
1/100 with all hand-written workflow
1/1000 with LIS printing of cassettes and labels, but human-applied
1/10,000 with barcoding and single piece workflow throughout the system and 
interfaces to instruments (stainers)

Makary MA et al. Surgical specimen identification errors  Surgery 2007 
Apr;141(4):450-5 
Nakhleh RE, et al. Amended reports ... Q-probes study of 1,667,547 accessioned 
cases ...  Arch Pathol Lab Med. 1998 Apr;122(4):303-9
Resar RK. Making noncatastrophic health care processes more reliable...  Health 
Serv Res. 2006; 41:1677-1689.



Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Tuesday, October 14, 2014 10:21 AM
To: Histonet Post (histonet@lists.utsouthwestern.edu)
Subject: [Histonet] Labeling Errors

Is there a national average or benchmark for "acceptable" labeling errors in 
Histology?

Laurie Colbert HT (ASCP)
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[Histonet] Labeling Errors

[Laurie Colbert]

Is there a national average or benchmark for "acceptable" labeling errors in 
Histology?

Laurie Colbert HT (ASCP)
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[Histonet] Slides from antibody validation

[Bell, Lynne]

Following antibody validation studies, do you keep your slides accessible for 
CAP to peruse during inspection?  Has anyone had CAP actually ask to see your 
slides?  I feel that keeping the validation forms, signed off by the medical 
director, is sufficient, but I would like other opinions.

As always, thanks for any and all guidance!

Lynne Bell, HT (ASCP)
Histology Team Leader
Central Vermont Medical Center
130 Fisher Road
Berlin, VT  05641
802-371-4923



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[Histonet] RE: white plastic scrapers

[Grantham, Andrea L - (algranth)]

Southeast Pathology was giving out scrapers at NSH this year but in case you 
didn't get one - go to the cooking section of Target or Walmart and see if they 
have a scraper to use on teflon surfaces. A squeegee works good on molten wax 
surfaces too. Otherwise, a snow scraper might work too.

Retired but lurking,
Andi G.


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Betsy Molinari 
[bmolin...@texasheart.org]
Sent: Tuesday, October 14, 2014 5:04 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] white plastic scrapers

Hi all,
Where can I purchase the whit plastic scrapers used to scrape the paraffin off 
embedding centers and other surfaces. I believe ours went out with the trash.
Thanks.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)







Betsy Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.org | 
www.texasheart.org



6770 Bertner Ave., MC 1-283, Houston, TX 77030



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Institute][THI
 News] [THI on Facebook]   [THI 
on Flicker]   [THI on Google] 
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[Histonet] recycled formalin

[Webb, Dorothy L]

How does everyone handle the use of recycled formalin in regard to tissue 
processors?  OK to use on the processors?  We have just recently installed the 
Creative Waste formalin recycler and I am looking for guidelines from those who 
have experience in this area.

Much thanks,  Dorothy Webb (Regions Histology, St. Paul, MN)



  

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Re: [Histonet] white plastic scrapers

[Sue]

Home Depot or Lowes sells plastic scrappers in the paint area.  I cannot see 
why we would pay top dollar for this item.  I also purchase brushed to clean 
paraffin off microtomes at the craft store.  50 cents each is much better than 
5 dollars. 
  
Sue Paturzo 
TJUH 
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RE: [Histonet] white plastic scrapers

[Shirley A. Powell]

Metal scrapers may remove some of the ceramic coatings on embedding centers, 
they do have plastic putty knives at Lowes or Home Depot or where ever you get 
hardware.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Tuesday, October 14, 2014 8:38 AM
To: Histonet@lists.utsouthwestern.edu; bmolin...@texasheart.org
Subject: RE: [Histonet] white plastic scrapers

You know what works better? A small, metal drywall taping/mud knife-maybe 3-4 
inches. They're more flexible than the plastic scrapers, and I think they 
actually pick up more paraffin per pass. You can pick these up at any hardware 
store.

Thanks,

Brian Cooper, HT (ASCP)
Histology Supervisor,
Path & Lab Medicine
Children's Hospital, Los Angeles

Sent from my Galaxy S3, so please forgive any weird typos . . .


-Original Message-
From: Betsy Molinari [bmolin...@texasheart.org]
Received: Tuesday, 14 Oct 2014, 5:08AM
To: Histonet@lists.utsouthwestern.edu [Histonet@lists.utsouthwestern.edu]
Subject: [Histonet] white plastic scrapers

Hi all,
Where can I purchase the whit plastic scrapers used to scrape the paraffin off 
embedding centers and other surfaces. I believe ours went out with the trash.
Thanks.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)







Betsy Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.org | 
www.texasheart.org



6770 Bertner Ave., MC 1-283, Houston, TX 77030



[Texas Heart 
Institute][THI
 News] [THI on Facebook]   [THI 
on Flicker]   [THI on Google] 
   [THI on Pinterest] 
  [THI on Twitter] 
  [THI on You Tube] 



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[Histonet] RE: Gram staining

[Gomez, Milton]

we use 5ml Picric Acid to 50 ml acetone and it works very well.  


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Cooper, Brian 
[bcoo...@chla.usc.edu]
Sent: Thursday, October 09, 2014 3:48 PM
To: Bitting, Angela K.; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Gram staining

We use the McDonald's Gram Stain kit from American Master Tech, which employs 
the use of a Modified Tartrazine counterstain.  Both our docs and techs love 
our Gram stains . . .

Here's the link to the product info.

http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=KTMGS

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bitting, Angela 
K.
Sent: Thursday, October 09, 2014 11:36 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gram staining


Our new Pathologist would like to see more yellow in the background of our Gram 
stain.  We use picric acid-acetone currently, which looks more pink than yellow 
most times. So  I'm wondering if a method using a Tartrazine counterstain would 
be better.
Any thoughts?


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[Histonet] RE: white plastic scrapers

[Weems, Joyce K.]

I've seen them at Bed Bath and Beyond  if you're talking about the little ones 
about 2 1/2 or 3 in squarish ones.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Betsy Molinari
Sent: Tuesday, October 14, 2014 8:04 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] white plastic scrapers

Hi all,
Where can I purchase the whit plastic scrapers used to scrape the paraffin off 
embedding centers and other surfaces. I believe ours went out with the trash.
Thanks.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)







Betsy Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.org | 
www.texasheart.org



6770 Bertner Ave., MC 1-283, Houston, TX 77030



[Texas Heart 
Institute][THI
 News] [THI on Facebook]   [THI 
on Flicker]   [THI on Google] 
   [THI on Pinterest] 
  [THI on Twitter] 
  [THI on You Tube] 




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[Histonet] THICK AND THIN SECTIONS ?

[Klaus Dern]

If you are using one of the following microtomes and the advance mechanism
is worn out
( too much play between spindle and spindle nut ), you could be faced with
purchasing a new microtome.

 REICHERT/JUNG2030
 LEICARM   2125
 LEICA2030 Biocut
 LEICA/JUNG  2035
 LEICA - CM   1800  Cryostat
 SAKURA - SRM  200

These models are no longer supported by the manufacturer, (no parts
availability).

Rather than replacing these excellent instruments, I have a PERMANENT
solution to fix this problem.

For information please contact:

Klaus Dern
Phone: 706 635-8840
E-Mail: klaus.der...@gmail.com
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[Histonet] RE: CD31 in rabbit tissue

[Bea DeBrosse-Serra]

We use CD31 from Spring BioScience (SP38).

Beatrice DeBrosse-Serra HT(ASCP)QIHC
Isis Pharmaceuticals
Antisense Drug Discovery
2855 Gazelle Ct.
Carlsbad, CA 92010
760-603-2371




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mandy Bell
Sent: Tuesday, October 14, 2014 5:46 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD31 in rabbit tissue

Hi,

We are trying to run CD31 in rabbit tissue.  We've tried a couple of different 
antibodies with no luck.  Hoping someone can point me in the right direction- 
does anyone have a CD31 they have had success with in rabbit tissue?

Thanks,

Mandy Bell,  HTL(ASCP)


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Re: [Histonet] CD31 in rabbit tissue

[Mehmet Fatih BOZKURT]

Hi,

Santacruz  Goat   sc-1506 works well on rabbit tissues. Good lucks.

On Tue, Oct 14, 2014 at 3:46 PM, Mandy Bell  wrote:

> Hi,
>
> We are trying to run CD31 in rabbit tissue.  We've tried a couple of
> different antibodies with no luck.  Hoping someone can point me in the
> right direction- does anyone have a CD31 they have had success with in
> rabbit tissue?
>
> Thanks,
>
> Mandy Bell,  HTL(ASCP)
>
>
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-- 
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Department of Pathology
Faculty of Veterinary Medicine
Afyon Kocatepe University
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-16173/16237

Asst. Prof. Dr. Mehmet Fatih BOZKURT
Manager of Veterinary Diagnostic Laboratory
Afyon Kocatepe University
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-16300-16301
vetana...@aku.edu.tr
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[Histonet] CD31 in rabbit tissue

[Mandy Bell]

Hi,

We are trying to run CD31 in rabbit tissue.  We've tried a couple of different 
antibodies with no luck.  Hoping someone can point me in the right direction- 
does anyone have a CD31 they have had success with in rabbit tissue?

Thanks,

Mandy Bell,  HTL(ASCP)


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RE: [Histonet] white plastic scrapers

[Cooper, Brian]

You know what works better? A small, metal drywall taping/mud knife-maybe 3-4 
inches. They're more flexible than the plastic scrapers, and I think they 
actually pick up more paraffin per pass. You can pick these up at any hardware 
store.

Thanks,

Brian Cooper, HT (ASCP)
Histology Supervisor,
Path & Lab Medicine
Children's Hospital, Los Angeles

Sent from my Galaxy S3, so please forgive any weird typos . . .


-Original Message-
From: Betsy Molinari [bmolin...@texasheart.org]
Received: Tuesday, 14 Oct 2014, 5:08AM
To: Histonet@lists.utsouthwestern.edu [Histonet@lists.utsouthwestern.edu]
Subject: [Histonet] white plastic scrapers

Hi all,
Where can I purchase the whit plastic scrapers used to scrape the paraffin off 
embedding centers and other surfaces. I believe ours went out with the trash.
Thanks.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)







Betsy Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.org | 
www.texasheart.org



6770 Bertner Ave., MC 1-283, Houston, TX 77030



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[Histonet] white plastic scrapers

[Betsy Molinari]

Hi all,
Where can I purchase the whit plastic scrapers used to scrape the paraffin off 
embedding centers and other surfaces. I believe ours went out with the trash.
Thanks.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston,TX 77030
832-355-6524 (lab)
832-355-6812 (fax)







Betsy Molinari
Senior Histology Research Technician
832-355-6524 | bmolin...@texasheart.org | 
www.texasheart.org



6770 Bertner Ave., MC 1-283, Houston, TX 77030



[Texas Heart 
Institute][THI
 News] [THI on Facebook]   [THI 
on Flicker]   [THI on Google] 
   [THI on Pinterest] 
  [THI on Twitter] 
  [THI on You Tube] 

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