[Histonet] Squamous cells staining on H&E and IHC

[Amber McKenzie]

Does anyone else have problems with what looks like squamous cells staining on 
your H&E's and IHC's?  I'm trying to figure out how to eliminate that problem 
in our lab...wear gloves while cutting?  Change out water bath several times 
during shifts? Any suggestions?  Thanks!

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[Histonet] Telepathology

[Martin, Gary]

We are being asked to provide evaluation of EUS (Endoscopic Ultra Sound)
specimen from  a remote location. We are provided with a microscope and
a laptop, does anyone know of a simple way to capture and transmit
images back to the pathology lab? 

Thank you. 

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RE: [Histonet] PT testing for ER/PR by IHC

[Joelle Weaver]

yes


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
> From: ihcman2...@hotmail.com
> To: histonet@lists.utsouthwestern.edu
> Date: Tue, 28 Oct 2014 12:55:34 -0500
> Subject: RE: [Histonet] PT testing for ER/PR by IHC
> 
> 
> All,
> 
>  
> 
> If you perform ER/PR staining by IHC, are you REQUIRED to participate in 
> Proficiency Testing like that provided by CAP?
> 
>  
> 
> Thank-you in advance,
> 
>  
> 
> Glen Dawson  BS, HT(ASCP), QIHC
> 
> Histology Technical Specialist
> 
> Mercy Health System
> 
> Janesville, WI
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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RE: [Histonet] PT testing for ER/PR by IHC

[Glen Dawson]

All,

 

If you perform ER/PR staining by IHC, are you REQUIRED to participate in 
Proficiency Testing like that provided by CAP?

 

Thank-you in advance,

 

Glen Dawson  BS, HT(ASCP), QIHC

Histology Technical Specialist

Mercy Health System

Janesville, WI
  
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[Histonet] RE: Change in xylene substitute

[Kukieza, Debra]

Hi Ronda,

I was faced with the same issue a few years ago.  We placed one gram of 
paraffin into equal amounts of the xylene substitutes and monitored how quickly 
and effectively the paraffin dissolved in each.   In the end we proved it was 
better to keep the substitute we were using as it completely dissolved the 
paraffin and the other did not.  The other actually left a rather sticky mess 
with the paraffin.

Debra
UPMC East
Histology Laboratory 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Tuesday, October 28, 2014 1:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 131, Issue 31

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Today's Topics:

   1. Position Posting: Laboratory Manager, Lab of Comparative
  Pathology, MSKCC/WCMC/RU, New York, NY (monet...@mskcc.org)
   2. Change in xylene substitute (Ronda Mire)
   3. RE: Working on Saturday (Mike Pence)
   4. Combined Case prefix (jessica.va...@hcahealthcare.com)
   5. RE: Combined Case prefix (Morken, Timothy)
   6. RE: Combined Case prefix (Tom McNemar)
   7. Storing specimens (Abbott, Tanya)
   8. TCR immunohistochemistry (Houston, Ronald)
   9. Re: Storing specimens (Michael Ann Jones)


--

Message: 1
Date: Mon, 27 Oct 2014 17:11:18 +
From: monet...@mskcc.org
Subject: [Histonet] Position Posting: Laboratory Manager, Lab of
Comparative Pathology, MSKCC/WCMC/RU, New York, NY
To: histonet@lists.utsouthwestern.edu
Message-ID:

<1e99c9d9f6f44a40bcd63551c0ab29e4b4f74...@smskpex10mbx3.mskcc.root.mskcc.org>

Content-Type: text/plain; charset=iso-8859-1

Position posting: Laboratory Manager, Lab. of Comparative Pathology, 
MSKCC/WCMC/RU, New York, NY

The Laboratory of Comparative Pathology (LCP) is a full service diagnostic lab 
offering clinical and anatomic pathology services. The laboratory serves the 
research communities and the animal resources of Memorial Sloan Kettering 
Cancer Center, Weill Cornell Medical College and The Rockefeller University, by 
performing a wide range of laboratory procedures in Microbiology, Hematology, 
Chemistry, Parasitology, Urinalysis and Serology on specimens from a variety of 
animal species. It also provides histology and immunohistochemistry services to 
a team of four comparative pathologists and to the research community.

To view more details about the position and to apply, please see:  
http://careers.mskcc.org/jobs/descriptions/manager-laboratory-of-comparative-pathology-new-york-new-york-job-4779417



S?bastien Monette, DMV, MVSc, DACVP
Head of Anatomic Pathology
Tri-Institutional Laboratory of Comparative Pathology Genetically Modified 
Animal Phenotyping Service Memorial Sloan-Kettering Cancer Center Weill Cornell 
Medical College The Rockefeller University

1275 York Avenue, Box 270, New York NY 10065
Phone: 646-888-2420
Fax: 646-422-0139
Email MSKCC: monet...@mskcc.org
Email WCMC: sem2...@med.cornell.edu
Web site: http://www.mskcc.org/research/comparative-medicine-pathology-0


 =

 

 Please note that this e-mail and any files transmitted from

 Memorial Sloan-Kettering Cancer Center may be privileged, confidential,

 and protected from disclosure under applicable law. If the reader of

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 you are hereby notified that any reading, dissemination, distribution,
 
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 and deleting this message, any attachments, and all copies and backups

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--



Message: 2
Date: Tue, 28 Oct 2014 07:55:15 -0400
From: "Ronda Mire" 
Subject: [Histonet] Change in xylene substitute
To: 
Message-ID: <01cff2a6$11498ec0$33dcac40$@org>
Content-Type: text/plain;   charset="us-ascii"

Hello netters,

 

Are all Xylene Substitutes the same?  My manager wants to change brands of 
Xylene Substitute to save money.  My fe

[Histonet] RE: Combined Case prefix

[Hannen, Valerie]

We only use one prefix for both AP and cytology( long ago we got rid of doing 
PAPS), has been this way for many years and don't recall any real problems.

Our computer system can differentiate specimen types(fluids vs tissues) for 
searches that we have set  up.



Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy
Sent: Tuesday, October 28, 2014 10:56 AM
To: 'jessica.va...@hcahealthcare.com'; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Combined Case prefix

We have several prefixes: surgical, cytology, eye pathology, hemepath, autopsy, 
research (we have many  more Specimen Classes but, thankfully, they do not all 
get separate prefixes). I'm not going to say it is absolutely necessary, just 
historical here. However, I have never been anywhere that did not have at least 
cyto and surgical use separate prefixes. I would guess that the larger the 
institution the more likely it is that there will be more separation. A small 
institution can combine everything without much problem. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC 
San Francisco Medical Center San Francisco, CA

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
jessica.va...@hcahealthcare.com
Sent: Tuesday, October 28, 2014 7:37 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Combined Case prefix

How many facilities have combined their prefixes? Meaning your pathology and 
cytology are using the same prefix so there is no difference when accessioning 
cases of pathology or cytology. What do you feel are the pro's and con's of 
this?

Jessica Vacca


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Re: [Histonet] Storing specimens

[Michael Ann Jones]

We run a query of reports (finalized) for that period of two weeks, and
any outstanding/pending report, we pull the bottle.
We use the storage bins for each day of the week - during gross we toss
the bottle into the storage bin for that day. We have two sets: Mon - Fri.
We rotate those in two cabinets and have a ³Current² sign we use and move
from one cabinet to another depending on which we dump. Once we dump the
bins (and disinfect them) we keep the Medical Waste bin for another week
(we keep our tissues for a total of three weeks) We don¹t have storage
room for 3 weeks worth rotation.
Did I make any sense?
:)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 10/28/14, 10:04 AM, "Abbott, Tanya" 
wrote:

>How does everyone handle storage of specimens for 2 weeks post sign out?
>We are a small lab (as is everyone on Histonet, I'm sure!!) and trying to
>figure out the best way to organize to make the flow go smoothly.
>Meaning, we are hoping not to have to check each specimen to determine it
>was signed out before we discard. Any suggestions?
>Thanks! Tanya
>
>Tanya G. Abbott RT (CSMLS)
>Manager Technologist, Histology/Cytology
>St. Joseph Medical Center
>Reading, PA 19603-0316
>ph  610-378-2635
>fax 610-898-5871
>email: tanyaabb...@catholichealth.net
>
>This electronic mail and any attached documents are intended solely for
>the named addressee(s) and contain confidential information. If you are
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[Histonet] TCR immunohistochemistry

[Houston, Ronald]

has anyone had any success with the antibodies available for TCR lymphocytes in 
human paraffin tissue?
Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster

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[Histonet] Storing specimens

[Abbott, Tanya]

How does everyone handle storage of specimens for 2 weeks post sign out? We are 
a small lab (as is everyone on Histonet, I'm sure!!) and trying to figure out 
the best way to organize to make the flow go smoothly. Meaning, we are hoping 
not to have to check each specimen to determine it was signed out before we 
discard. Any suggestions?
Thanks! Tanya

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabb...@catholichealth.net

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[Histonet] RE: Combined Case prefix

[Tom McNemar]

We have a few different prefixes.  We use Meditech and have different 
enter/edit screens depending on whether the specimen is a cytology or surgical. 
 It also help in searches and gathering statistics if you can limit to a 
specific prefix.

Tom Mc Nemar, HT(ASCP)
Histology Supervisor
(740) 348-4163
Licking Memorial Hospital
1320 West Main Street
Newark, OH  43055


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
jessica.va...@hcahealthcare.com
Sent: Tuesday, October 28, 2014 10:37 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Combined Case prefix

How many facilities have combined their prefixes? Meaning your pathology and 
cytology are using the same prefix so there is no difference when accessioning 
cases of pathology or cytology. What do you feel are the pro's and con's of 
this?

Jessica Vacca


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[Histonet] RE: Combined Case prefix

[Morken, Timothy]

We have several prefixes: surgical, cytology, eye pathology, hemepath, autopsy, 
research (we have many  more Specimen Classes but, thankfully, they do not all 
get separate prefixes). I'm not going to say it is absolutely necessary, just 
historical here. However, I have never been anywhere that did not have at least 
cyto and surgical use separate prefixes. I would guess that the larger the 
institution the more likely it is that there will be more separation. A small 
institution can combine everything without much problem. 

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential, 
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message.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
jessica.va...@hcahealthcare.com
Sent: Tuesday, October 28, 2014 7:37 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Combined Case prefix

How many facilities have combined their prefixes? Meaning your pathology and 
cytology are using the same prefix so there is no difference when accessioning 
cases of pathology or cytology. What do you feel are the pro's and con's of 
this?

Jessica Vacca


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[Histonet] Combined Case prefix

[Jessica.Vacca]

How many facilities have combined their prefixes? Meaning your pathology and 
cytology are using the same prefix so there is no difference when accessioning 
cases of pathology or cytology. What do you feel are the pro's and con's of 
this?

Jessica Vacca


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[Histonet] RE: Working on Saturday

[Mike Pence]

Same here.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Thursday, October 23, 2014 1:01 PM
To: 'Weems, Joyce K.'; 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Working on Saturday

Since ANP 22983 has been changed to state that breast fixation in formalin is 
between 6-72 hours, we no longer have staff come in on weekends. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Thursday, October 23, 2014 1:44 PM
To: 'Smallwood, Lorraine'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Working on Saturday

We stopped Sat a few years ago. We have someone on call if something is needed. 
We trained the clinical lab to take the breast cases off the processor so we 
rarely need to come in.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Smallwood, 
Lorraine
Sent: Thursday, October 23, 2014 1:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Working on Saturday

We are a 450 bed hospital.  Due to our size and volume of work, our work week 
is Monday-Saturday. Because of healthcare reform, budget cuts and staffing 
shortage, we are faced with closing the department on Saturday (!!Histotechs 
are happy!!). One tech will continue to come in on Saturday to process any 
breast specimens received.   I am curious to find out what is the norm 
concerning working on Saturdays.  How many other Histology departments work on 
Saturday?  Thanks in advance for your opinions!

Lorraine Smallwood MScEd, PA(ASCP)
BB(ASCP)IHC, MLS(ASCP)HT, MT(AMT)
Pathologist Assistant


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[Histonet] Change in xylene substitute

[Ronda Mire]

Hello netters,

 

Are all Xylene Substitutes the same?  My manager wants to change brands of
Xylene Substitute to save money.  My feeling is that I will need to validate
processing, staining, ihc etc  if the chemical formula is different from
the substitute we currently use.  If it's the same then no harm, no foul.
What are your thoughts?

 

Ronda

 

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