Re: [Histonet] RE: Paraffin Temperature Checks

[b427297]

I like to use a chart recorder on paraffin dispensers in case the thing goes 
whacko over a weekend and boils the paraffin, then goes back to normal before 
anyone notices.

Sent from my iPhone

> On Jan 8, 2015, at 6:10 PM, Elizabeth Chlipala  wrote:
> 
> All of our logs are use logs so we record when we use any piece of equipment 
> and any QC or maintenance if required, we do not record or document anything 
> if we do not use it, other than lab temperature and humidity which we record 
> daily.
> 
> Liz
> 
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> l...@premierlab.com
> www.premierlab.com
> 
> March 10, 2014 is Histotechnology Professionals Day
> 
> Ship to Address:
> 
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
> Sent: Thursday, January 08, 2015 4:34 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Paraffin Temperature Checks
> 
> For those of you out there that are fortunate enough to have tissue 
> processors or embedding centers that are used less often than daily-like 
> maybe even weekly (or even less frequently,) how often are you checking and 
> documenting the paraffin temperatures on said pieces of equipment?  For our 
> daily equipment, it's no big deal obviously.  But we have a "research" 
> processor and embedding center that doesn't get used often-sometimes for a 
> week or two, and if we're not touching the machine, it seems overkill to 
> document paraffin temps daily.
> 
> Thanks,
> 
> Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology 
> and Laboratory Medicine Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> Ph: 323.361.3357 Pager: 213-209-0184
> bcoo...@chla.usc.edu
> 
> 
> 
> -
> CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is 
> for the sole use of the intended recipient(s) and may contain confidential or 
> legally privileged information. Any unauthorized review, use, disclosure or 
> distribution is prohibited. If you are not the intended recipient, please 
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> 
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[Histonet] RE: Paraffin Temperature Checks

[Elizabeth Chlipala]

All of our logs are use logs so we record when we use any piece of equipment 
and any QC or maintenance if required, we do not record or document anything if 
we do not use it, other than lab temperature and humidity which we record daily.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Thursday, January 08, 2015 4:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin Temperature Checks

For those of you out there that are fortunate enough to have tissue processors 
or embedding centers that are used less often than daily-like maybe even weekly 
(or even less frequently,) how often are you checking and documenting the 
paraffin temperatures on said pieces of equipment?  For our daily equipment, 
it's no big deal obviously.  But we have a "research" processor and embedding 
center that doesn't get used often-sometimes for a week or two, and if we're 
not touching the machine, it seems overkill to document paraffin temps daily.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] RE: Paraffin Temperature Checks

[Morken, Timothy]

Brain, we write in NIU for Not in Use if it is not used on a particular day. 
However, you may want to do checks a couple times per week just to be sure it 
is working so you don't find solidified paraffin the day you want to use it! 
Since it takes just as much time to write NIU as it does to write down the 
temp, you might as well do it!

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian
Sent: Thursday, January 08, 2015 3:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin Temperature Checks

For those of you out there that are fortunate enough to have tissue processors 
or embedding centers that are used less often than daily-like maybe even weekly 
(or even less frequently,) how often are you checking and documenting the 
paraffin temperatures on said pieces of equipment?  For our daily equipment, 
it's no big deal obviously.  But we have a "research" processor and embedding 
center that doesn't get used often-sometimes for a week or two, and if we're 
not touching the machine, it seems overkill to document paraffin temps daily.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and 
Laboratory Medicine Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] Paraffin Temperature Checks

[Cooper, Brian]

For those of you out there that are fortunate enough to have tissue processors 
or embedding centers that are used less often than daily-like maybe even weekly 
(or even less frequently,) how often are you checking and documenting the 
paraffin temperatures on said pieces of equipment?  For our daily equipment, 
it's no big deal obviously.  But we have a "research" processor and embedding 
center that doesn't get used often-sometimes for a week or two, and if we're 
not touching the machine, it seems overkill to document paraffin temps daily.

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] plant processing

[Jennifer MacDonald]

I have a colleague at a neighboring school that would like to process 
plant tissue for materials for his botany class.  Is there a good resource 
for plant processing?  Is there anyone in southern California that is 
processing plant materials?
Thank you,
Jennifer
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[Histonet] RE: ASP300S or VIP 6

[Hugh Luk]

Hi Jim,

Both machines have given us years of service and we (pretty) highly recommend 
either.

However, part of your decision should encompass service.  I recommend buying 
two of the same.  Validation was easy (well, sort-of) on either machine.

Notes and problems we've encountered with our machines:

ASP300(S) "Thermal fuses" blow occasionally (if you do not premelt ALL the 
paraffin).   Maybe 1-2/year.  Also had a problem with a clog during the 
cleaning cycle (but resolved itself as was more a 'lazy tech' issue).  
Processing baskets are 3 x 100 and there are three paraffin chambers.  Reagent 
containers "Low Level" are > 1 gallon (awe, have to get another bottle, to top 
off the container).

VIP6:  Our touchscreen malfunctioned one day after the warranty expired.  
Otherwise, the machine processes reagents similar to the ASP300 S and VIP5.  
Processing baskets are 2 x 150 and there are four, removable paraffin 
containers.  Reagent containers are 1.0 gallons.

I consider there problems small as our Biomed and back-up processors saved us.  
Best of luck making your choice.
Hugh
Hawaii


> --
> Date: Wed, 7 Jan 2015 18:24:42 +
> From: "Vickroy, James" 
> Subject: [Histonet] ASP300S or VIP 6
> To: "histonet@lists.utsouthwestern.edu"
>   
> Message-ID:
>   <9b1a1501a800064397369bd8072e6bca96a...@e2k10db.springfieldclinic.com>
> Content-Type: text/plain; charset="us-ascii"
> 
> 
> Buying two automated tissue processors for new lab.   I have always used the 
> VIP tissue processors, can anyone comment on a side by side comparison 
> between the Leica and the VIP 6?
> 
> thanks
> 
> Jim Vickroy
> Histology Manager
> Springfield Clinic, Main Campus, East Building
> 1025 South 6th Street
> Springfield, Illinois  62703
> Office:  217-528-7541, Ext. 15121
> Email:  jvick...@springfieldclinic.com
> 
> 
> This electronic message contains information from Springfield Clinic, LLP 
> that may be confidential, privileged, and/or sensitive. This information is 
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> distribution, or action taken on the contents of this information is strictly 
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Re: And other crazy stuff. RE: [Histonet] cutting honey bees

[Jennifer MacDonald]

Termites for a science fair project.
anchovy from a pizza to pull a joke on a pathologist
chicken bone from dim sum chicken feet, again to pull one over on a 
pathologist.  Not decalcified, embedded in resin.



From:   "Morken, Timothy" 
To: Patsy Ruegg , Roberta Horner , 
"Douglas Gregg" , "Histonet@Lists. Edu" 

Date:   01/08/2015 12:00 PM
Subject:And other crazy stuff.  RE: [Histonet] cutting honey bees
Sent by:histonet-boun...@lists.utsouthwestern.edu



You crazy research people...OK, so what is the craziest thing you ever had 
to cut, or were asked to cut?

For me, not too bad, but embedding for EM and sectioning a single oocyte 
that was nearly microscopic. I'll just say it took a LOT of thick sections 
too face down to it without actually cutting through it.


Open the floodgates

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, January 06, 2015 11:13 AM
To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
Subject: RE: [Histonet] cutting honey bees

for the whole bee I probably would process and embed it in glycol 
methacrylate (gma) it is much harder and would give better sections, we 
have done zebra fish and several other harder tissues including calcified 
bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> From: r...@psu.edu
> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
> Date: Sat, 3 Jan 2015 23:15:33 +
> Subject: RE: [Histonet] cutting honey bees
> CC: 
> 
> I sectioned and stained honey bee and yellow jacket stingers years ago. 
They wanted to show the difference between the stingers.  I wasn't sure 
what to do so I processed and handled like everything else.  I was able to 
get some good sections.  I put 6 stingers in each block and cut several 
sections figuring there should be at least one good stinger in each block 
and it worked.
> Roberta Horner
> Penn State University
> Animal Diagnostic Lab
> 
> From: histonet-boun...@lists.utsouthwestern.edu 
> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg 
> [classic...@gmail.com]
> Sent: Saturday, January 03, 2015 6:08 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] cutting honey bees
> 
> Has anyone had experience embedding and cutting honey bees. I am sure 
> there are some issues with the harder exoskeleton. Would that have to 
> be dissected away first. I am considering helping a student with a 
> science fair project on bees.
> 
> Douglas Gregg
> Veterianary pathologist
> 
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RE: [Histonet] Decal acquired by StatLab

[Patsy Ruegg]

that is very interesting.  I always used Decal Chemical products really liked 
their formic acid Immunocal reagent.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> Date: Thu, 8 Jan 2015 15:44:26 -0500
> From: rsrichm...@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Decal acquired by StatLab
> 
> I just got an e-mail noting that
> 
> "StatLab Medical Products, the leading manufacturer, developer and
> distributor of cost-effective histology, cytology and immunohistochemistry
> consumable supplies, is pleased to announce the acquisition of Decal
> Chemical Corporation of Tallman, NY."
> 
> Good time to be reminded that Decal™ is a trademarked name, and is not the
> generic term for any bottle of decalcifier on the shelf.
> 
> Decal was the most commonly used proprietary decalcifier when I started out
> in pathology fifty years ago. In a day when most labs prepared most of
> their own reagents, they always bought proprietary decalcifiers.
> 
> Nowadays people buy all solutions. I think the Herrn Inschpektors get you
> if you home-brew.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville TN
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[Histonet] Decal acquired by StatLab

[Bob Richmond]

I just got an e-mail noting that

"StatLab Medical Products, the leading manufacturer, developer and
distributor of cost-effective histology, cytology and immunohistochemistry
consumable supplies, is pleased to announce the acquisition of Decal
Chemical Corporation of Tallman, NY."

Good time to be reminded that Decal™ is a trademarked name, and is not the
generic term for any bottle of decalcifier on the shelf.

Decal was the most commonly used proprietary decalcifier when I started out
in pathology fifty years ago. In a day when most labs prepared most of
their own reagents, they always bought proprietary decalcifiers.

Nowadays people buy all solutions. I think the Herrn Inschpektors get you
if you home-brew.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: And other crazy stuff. RE: [Histonet] cutting honey bees

[Paula Sicurello]

You asked for it:

squid tentacle tip, frog oocyte, serial sections through a entire zebra
fish embryo, honey bee eyes, harbor seal artery, Andean mummy muscle (flaky
little boogers), probably lots of others that I can't remember off the top
of my head.

Paula

On Thu, Jan 8, 2015 at 3:20 PM, Michael Ann Jones 
wrote:

> We did a goldfish once, interesting microscopically and difficult for
> peeling (lots of keratin?)
> Michael Ann Jones, HT (ASCP)
> Histology Manager
> Metropath
> 7444 W. Alaska Dr. #250
> Lakewood, CO 80226
> 303.634.2511
> mjo...@metropath.com
>
>
>
>
> On 1/6/15, 12:23 PM, "Morken, Timothy"  wrote:
>
> >You crazy research people...OK, so what is the craziest thing you ever
> >had to cut, or were asked to cut?
> >
> >For me, not too bad, but embedding for EM and sectioning a single oocyte
> >that was nearly microscopic. I'll just say it took a LOT of thick
> >sections too face down to it without actually cutting through it.
> >
> >
> >Open the floodgates
> >
> >Tim Morken
> >
> >-Original Message-
> >From: histonet-boun...@lists.utsouthwestern.edu
> >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy
> >Ruegg
> >Sent: Tuesday, January 06, 2015 11:13 AM
> >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
> >Subject: RE: [Histonet] cutting honey bees
> >
> >for the whole bee I probably would process and embed it in glycol
> >methacrylate (gma) it is much harder and would give better sections, we
> >have done zebra fish and several other harder tissues including calcified
> >bone in GMA.
> >
> >Cheers,
> >Patsy
> >
> >Patsy Ruegg, HT(ASCP)QIHC
> >Ruegg IHC Consulting
> >40864 E Arkansas Ave
> >Bennett, CO 80102
> >H 303-644-4538
> >C 720-281-5406
> >prueg...@hotmail.com
> >
> >
> >
> >> From: r...@psu.edu
> >> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
> >> Date: Sat, 3 Jan 2015 23:15:33 +
> >> Subject: RE: [Histonet] cutting honey bees
> >> CC:
> >>
> >> I sectioned and stained honey bee and yellow jacket stingers years ago.
> >> They wanted to show the difference between the stingers.  I wasn't sure
> >>what to do so I processed and handled like everything else.  I was able
> >>to get some good sections.  I put 6 stingers in each block and cut
> >>several sections figuring there should be at least one good stinger in
> >>each block and it worked.
> >> Roberta Horner
> >> Penn State University
> >> Animal Diagnostic Lab
> >> 
> >> From: histonet-boun...@lists.utsouthwestern.edu
> >> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg
> >> [classic...@gmail.com]
> >> Sent: Saturday, January 03, 2015 6:08 PM
> >> To: histonet@lists.utsouthwestern.edu
> >> Subject: [Histonet] cutting honey bees
> >>
> >> Has anyone had experience embedding and cutting honey bees. I am sure
> >> there are some issues with the harder exoskeleton. Would that have to
> >> be dissected away first. I am considering helping a student with a
> >> science fair project on bees.
> >>
> >> Douglas Gregg
> >> Veterianary pathologist
> >>
> >> ___
> >> Histonet mailing list
> >> Histonet@lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >>
> >> ___
> >> Histonet mailing list
> >> Histonet@lists.utsouthwestern.edu
> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>  ___
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RE: And other crazy stuff. RE: [Histonet] cutting honey bees

[Patsy Ruegg]

we once played an april's fool joke on our Heme Pathologist.  We took the wings 
off a fly and embedded it in plastic (GMA) sectioned and stained it. Grossly It 
looked similar to the bone marrow core biopsies we did.  Heme pathologist are 
notorious for sticking slides under the microscope on high power without 
looking at them grossly.  We said we needed him to consult on this really 
strange bone marrow core because we couldn't figure out what the patient had.  
Most of our patients at the time were patients with leukemia or lymphoma.  Sure 
enough he stuck the slide under high power and probably even under oil 
immersion.  Trying to look at the morphology of individual cells.  He was 
stumped and was going to show it to another colleague, so we had to confess 
that it was a fly and this was a AF joke.  We thought he would have thought we 
were clever and laugh about it but he did not think it was funny.  We never did 
anything like that again.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> Subject: RE: And other crazy stuff.  RE: [Histonet] cutting honey bees
> Date: Thu, 8 Jan 2015 15:27:58 -0500
> From: dhew...@hvhs.org
> To: mjo...@metropath.com; timothy.mor...@ucsf.edu; prueg...@hotmail.com; 
> r...@psu.edu; classic...@gmail.com; histonet@lists.utsouthwestern.edu
> 
> I have done a stink bug, spider and a few other creepy crawlers for my
> kids to look at under the scope, they have no idea what they are looking
> at but still love it.
> 
> Daniel Hewitt
> Histology Supervisor, HVS
> 412-749-7371
> 
> This email, including any attachments, is for the sole use of the
> intended recipient(s) and may contain confidential and privileged
> information.  Any unauthorized review, use, disclosure or distribution
> is prohibited.  If you are not the intended recipient, or an agent
> responsible for delivering the message to the intended recipient, please
> contact the sender by reply e-mail and delete and destroy all copies of
> the original message, including attachments.
> 
> Please note that any views or opinions presented in this e-mail are
> solely those of the author and do not necessarily represent those of
> Heritage Valley Health System.  The integrity and security of this
> message cannot be guaranteed on the internet.
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
> Ann Jones
> Sent: Thursday, January 08, 2015 3:20 PM
> To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg;
> 'histonet@lists.utsouthwestern.edu'
> Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees
> 
> We did a goldfish once, interesting microscopically and difficult for
> peeling (lots of keratin?)
> Michael Ann Jones, HT (ASCP)
> Histology Manager
> Metropath
> 7444 W. Alaska Dr. #250
> Lakewood, CO 80226
> 303.634.2511
> mjo...@metropath.com
> 
> 
> 
> 
> On 1/6/15, 12:23 PM, "Morken, Timothy"  wrote:
> 
> >You crazy research people...OK, so what is the craziest thing you ever
> >had to cut, or were asked to cut?
> >
> >For me, not too bad, but embedding for EM and sectioning a single
> oocyte
> >that was nearly microscopic. I'll just say it took a LOT of thick
> >sections too face down to it without actually cutting through it.
> >
> >
> >Open the floodgates
> >
> >Tim Morken
> >
> >-Original Message-
> >From: histonet-boun...@lists.utsouthwestern.edu
> >[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy
> >Ruegg
> >Sent: Tuesday, January 06, 2015 11:13 AM
> >To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
> >Subject: RE: [Histonet] cutting honey bees
> >
> >for the whole bee I probably would process and embed it in glycol
> >methacrylate (gma) it is much harder and would give better sections, we
> >have done zebra fish and several other harder tissues including
> calcified
> >bone in GMA.
> >
> >Cheers,
> >Patsy
> >
> >Patsy Ruegg, HT(ASCP)QIHC
> >Ruegg IHC Consulting
> >40864 E Arkansas Ave
> >Bennett, CO 80102
> >H 303-644-4538
> >C 720-281-5406
> >prueg...@hotmail.com
> >
> >
> >
> >> From: r...@psu.edu
> >> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
> >> Date: Sat, 3 Jan 2015 23:15:33 +
> >> Subject: RE: [Histonet] cutting honey bees
> >> CC: 
> >> 
> >> I sectioned and stained honey bee and yellow jacket stingers years
> ago.
> >> They wanted to show the difference between the stingers.  I wasn't
> sure
> >>what to do so I processed and handled like everything else.  I was
> able
> >>to get some good sections.  I put 6 stingers in each block and cut
> >>several sections figuring there should be at least one good stinger in
> >>each block and it worked.
> >> Roberta Horner
> >> Penn State University
> >> Animal Diagnostic Lab
> >> 
> >> From: histonet-boun...@lists.utsouthwestern.edu
> >> [his

RE: And other crazy stuff. RE: [Histonet] cutting honey bees

[Daniel Hewitt]

I have done a stink bug, spider and a few other creepy crawlers for my
kids to look at under the scope, they have no idea what they are looking
at but still love it.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Michael
Ann Jones
Sent: Thursday, January 08, 2015 3:20 PM
To: Morken, Timothy; Patsy Ruegg; Roberta Horner; Douglas Gregg;
'histonet@lists.utsouthwestern.edu'
Subject: Re: And other crazy stuff. RE: [Histonet] cutting honey bees

We did a goldfish once, interesting microscopically and difficult for
peeling (lots of keratin?)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 1/6/15, 12:23 PM, "Morken, Timothy"  wrote:

>You crazy research people...OK, so what is the craziest thing you ever
>had to cut, or were asked to cut?
>
>For me, not too bad, but embedding for EM and sectioning a single
oocyte
>that was nearly microscopic. I'll just say it took a LOT of thick
>sections too face down to it without actually cutting through it.
>
>
>Open the floodgates
>
>Tim Morken
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy
>Ruegg
>Sent: Tuesday, January 06, 2015 11:13 AM
>To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
>Subject: RE: [Histonet] cutting honey bees
>
>for the whole bee I probably would process and embed it in glycol
>methacrylate (gma) it is much harder and would give better sections, we
>have done zebra fish and several other harder tissues including
calcified
>bone in GMA.
>
>Cheers,
>Patsy
>
>Patsy Ruegg, HT(ASCP)QIHC
>Ruegg IHC Consulting
>40864 E Arkansas Ave
>Bennett, CO 80102
>H 303-644-4538
>C 720-281-5406
>prueg...@hotmail.com
>
>
>
>> From: r...@psu.edu
>> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
>> Date: Sat, 3 Jan 2015 23:15:33 +
>> Subject: RE: [Histonet] cutting honey bees
>> CC: 
>> 
>> I sectioned and stained honey bee and yellow jacket stingers years
ago.
>> They wanted to show the difference between the stingers.  I wasn't
sure
>>what to do so I processed and handled like everything else.  I was
able
>>to get some good sections.  I put 6 stingers in each block and cut
>>several sections figuring there should be at least one good stinger in
>>each block and it worked.
>> Roberta Horner
>> Penn State University
>> Animal Diagnostic Lab
>> 
>> From: histonet-boun...@lists.utsouthwestern.edu
>> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas
Gregg
>> [classic...@gmail.com]
>> Sent: Saturday, January 03, 2015 6:08 PM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] cutting honey bees
>> 
>> Has anyone had experience embedding and cutting honey bees. I am sure
>> there are some issues with the harder exoskeleton. Would that have to
>> be dissected away first. I am considering helping a student with a
>> science fair project on bees.
>> 
>> Douglas Gregg
>> Veterianary pathologist
>> 
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> ___
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>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
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>
>___
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Re: And other crazy stuff. RE: [Histonet] cutting honey bees

[Michael Ann Jones]

We did a goldfish once, interesting microscopically and difficult for
peeling (lots of keratin?)
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com




On 1/6/15, 12:23 PM, "Morken, Timothy"  wrote:

>You crazy research people...OK, so what is the craziest thing you ever
>had to cut, or were asked to cut?
>
>For me, not too bad, but embedding for EM and sectioning a single oocyte
>that was nearly microscopic. I'll just say it took a LOT of thick
>sections too face down to it without actually cutting through it.
>
>
>Open the floodgates
>
>Tim Morken
>
>-Original Message-
>From: histonet-boun...@lists.utsouthwestern.edu
>[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy
>Ruegg
>Sent: Tuesday, January 06, 2015 11:13 AM
>To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
>Subject: RE: [Histonet] cutting honey bees
>
>for the whole bee I probably would process and embed it in glycol
>methacrylate (gma) it is much harder and would give better sections, we
>have done zebra fish and several other harder tissues including calcified
>bone in GMA.
>
>Cheers,
>Patsy
>
>Patsy Ruegg, HT(ASCP)QIHC
>Ruegg IHC Consulting
>40864 E Arkansas Ave
>Bennett, CO 80102
>H 303-644-4538
>C 720-281-5406
>prueg...@hotmail.com
>
>
>
>> From: r...@psu.edu
>> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
>> Date: Sat, 3 Jan 2015 23:15:33 +
>> Subject: RE: [Histonet] cutting honey bees
>> CC: 
>> 
>> I sectioned and stained honey bee and yellow jacket stingers years ago.
>> They wanted to show the difference between the stingers.  I wasn't sure
>>what to do so I processed and handled like everything else.  I was able
>>to get some good sections.  I put 6 stingers in each block and cut
>>several sections figuring there should be at least one good stinger in
>>each block and it worked.
>> Roberta Horner
>> Penn State University
>> Animal Diagnostic Lab
>> 
>> From: histonet-boun...@lists.utsouthwestern.edu
>> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg
>> [classic...@gmail.com]
>> Sent: Saturday, January 03, 2015 6:08 PM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] cutting honey bees
>> 
>> Has anyone had experience embedding and cutting honey bees. I am sure
>> there are some issues with the harder exoskeleton. Would that have to
>> be dissected away first. I am considering helping a student with a
>> science fair project on bees.
>> 
>> Douglas Gregg
>> Veterianary pathologist
>> 
>> ___
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> 
>> ___
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>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
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>Histonet@lists.utsouthwestern.edu
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>
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[Histonet] RE: PASD muscle stains

[Cooper, Brian]

Same here. 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcoo...@chla.usc.edu 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Linda Prasad 
(SCHN)
Sent: Tuesday, January 06, 2015 2:58 PM
To: 'Tiffany Passaro'; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: PASD muscle stains

Usually we do the DiPAS stain on muscle frozen section. Air dried for 10 
minutes. Don't use any fixatives :)


Linda Prasad, MSc, BSc

Senior Scientist | Histopathology
t: 02 9845 3306 | f: 02 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au
m: 0425 314 267
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead 
NSW 2145, AUSTRALIA



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tiffany Passaro
Sent: Tuesday, 6 January 2015 10:23 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] PASD muscle stains

Greetings,


I am looking for fixatives that others are using in their labs 
for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 
10% NBF. Thanks in advance for any info on this.

Tiffany
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-
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or distribution is prohibited. If you are not the intended recipient, please
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[Histonet] RE: PASD muscle stains

[Linda Prasad (SCHN)]

Usually we do the DiPAS stain on muscle frozen section. Air dried for 10 
minutes. Don't use any fixatives :)


Linda Prasad, MSc, BSc

Senior Scientist | Histopathology
t: 02 9845 3306 | f: 02 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: 
www.schn.health.nsw.gov.au
m: 0425 314 267
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tiffany Passaro
Sent: Tuesday, 6 January 2015 10:23 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] PASD muscle stains

Greetings,


I am looking for fixatives that others are using in their labs 
for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 
10% NBF. Thanks in advance for any info on this.

Tiffany
DISCLAIMER:

This message is intended for the sole use of the addressee, and may contain 
information that is privileged, confidential and exempt from disclosure under 
applicable law. If you are not the addressee you are hereby notified that you 
may not use, copy, disclose, or distribute to anyone the message or any 
information contained in the message. If you have received this message in 
error, please immediately advise the sender by reply email and delete this 
message.
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This note also confirms that this email message has been virus scanned and 
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Network accepts no liability for any consequential damage resulting from email 
containing computer viruses.
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[Histonet] RE: Research cases

[Morken, Timothy]

We do a lot of research work , but generally in-house projects. We charge the  
technical-only  fee. For in-house academic research we offer a steep discount. 
For commercial work we have a client price, depending on how much work we do 
for them. 


Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential, 
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intended recipient, you may not use, copy, or distribute this email message or 
its attachments. If you believe you have received this email message in error, 
please contact the sender by reply email and destroy all copies of the original 
message.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Thursday, January 08, 2015 6:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Research cases

Good morning everyone!

I wanted to ask what others are doing about charges when blocks & slides are 
being requested for research purposes. We have been asked to provide a cost for 
this service, because the research companies are willing to pay the lab for our 
time processing, embedding, and cutting slides specifically for this purpose. 
Any guidance in this would be greatly appreciated!


Toni Rathborne
Pathology Supervisor

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[Histonet] zirconia ceramic knife 2015!

[peter]

 Hi  manager, 


 Good day!
Need zirconia ceramic knife, we are professional Chinese factory .
 If  have interest, pls contact me.
 3''-7''  knife  /   knife set/  vegetable peeler, scisssor   /   fork, 
spoon   /noble gift set


welcomed for inquiry.
K.R
Peter chan



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[Histonet] RE: PASD muscle stains

[Morken, Timothy]

Tiffany, we use 10% acetic acid per the AFIP procedure (1968 edition)


Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tiffany Passaro
Sent: Monday, January 05, 2015 3:23 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] PASD muscle stains

Greetings,


I am looking for fixatives that others are using in their labs 
for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 
10% NBF. Thanks in advance for any info on this.

Tiffany
DISCLAIMER:

This message is intended for the sole use of the addressee, and may contain 
information that is privileged, confidential and exempt from disclosure under 
applicable law. If you are not the addressee you are hereby notified that you 
may not use, copy, disclose, or distribute to anyone the message or any 
information contained in the message. If you have received this message in 
error, please immediately advise the sender by reply email and delete this 
message.
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And other crazy stuff. RE: [Histonet] cutting honey bees

[Morken, Timothy]

You crazy research people...OK, so what is the craziest thing you ever had to 
cut, or were asked to cut?

For me, not too bad, but embedding for EM and sectioning a single oocyte that 
was nearly microscopic. I'll just say it took a LOT of thick sections too face 
down to it without actually cutting through it.


Open the floodgates

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, January 06, 2015 11:13 AM
To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu
Subject: RE: [Histonet] cutting honey bees

for the whole bee I probably would process and embed it in glycol methacrylate 
(gma) it is much harder and would give better sections, we have done zebra fish 
and several other harder tissues including calcified bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> From: r...@psu.edu
> To: classic...@gmail.com; histonet@lists.utsouthwestern.edu
> Date: Sat, 3 Jan 2015 23:15:33 +
> Subject: RE: [Histonet] cutting honey bees
> CC: 
> 
> I sectioned and stained honey bee and yellow jacket stingers years ago.  They 
> wanted to show the difference between the stingers.  I wasn't sure what to do 
> so I processed and handled like everything else.  I was able to get some good 
> sections.  I put 6 stingers in each block and cut several sections figuring 
> there should be at least one good stinger in each block and it worked.
> Roberta Horner
> Penn State University
> Animal Diagnostic Lab
> 
> From: histonet-boun...@lists.utsouthwestern.edu 
> [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg 
> [classic...@gmail.com]
> Sent: Saturday, January 03, 2015 6:08 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] cutting honey bees
> 
> Has anyone had experience embedding and cutting honey bees. I am sure 
> there are some issues with the harder exoskeleton. Would that have to 
> be dissected away first. I am considering helping a student with a 
> science fair project on bees.
> 
> Douglas Gregg
> Veterianary pathologist
> 
> ___
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> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
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RE: [Histonet] Anyone using Biogenex stainers

[Patsy Ruegg]

I was curious about that too.  Don't they have that large fancy machine they 
claim does ISH and everything?


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com



> From: timothy.mor...@ucsf.edu
> To: histonet@lists.utsouthwestern.edu
> Date: Thu, 8 Jan 2015 18:43:41 +
> Subject: [Histonet] Anyone using Biogenex stainers
> 
> Does anyone use a Biogenex stainer these days? They used to be a premier 
> company but seem to have disappeared over the last decade.  They still 
> display at NSH and have ads in various places but am not aware of any lab 
> that uses one.
> 
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> Box 1656
> 505 Parnassus Ave
> San Francisco, CA 94143
> USA
> 
> 415.514-6042  (office)
> tim.mor...@ucsfmedctr.org
> 
> CONFIDENTIALITY NOTICE: This email message, including any attachments, is for 
> the sole use of the intended recipient(s) and may contain confidential, 
> proprietary, and/or privileged information protected by law. If you are not 
> the intended recipient, you may not use, copy, or distribute this email 
> message or its attachments. If you believe you have received this email 
> message in error, please contact the sender by reply email and destroy all 
> copies of the original message.
> 
> 
> 
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[Histonet] Usage of Thermo Cassette and Slde Printer

[Vickroy, James]

Could anyone comment on the Thermo Printmate and Slide Mate?   I am considering 
going to it for labeling of cassettes and slides.

Thanks

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com


This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
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RE: [Histonet] New 88344 CPT code question

[Murray Anderson]

Dear Michelle,

For CMS:
All tests are still billed "per specimen-per procedure"
All "G" codes have been replaced with the following:
88342 - 1st initial antibody stain procedure
88341 - each additional antibody stain procedure
88344 - each Multiplex stain procedure

Assuming that your block 1 and block 2 came from the same specimen...

Your Scenario:
Stain1 - Block A1 = 1 x 88344
Stain1 - Block A2 = cannot bill
Stain 2 - Block A1 = 1 x 88344

Regards,

Murray Anderson

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Thursday, January 08, 2015 7:17 AM
To: Histonet
Subject: [Histonet] New 88344 CPT code question

Hello Histonetters :)

With regards to billing for multiplex IHC stains, I have a scenario and 
question.
Scenario: 1 container, 2 blocks, 2 different cocktail IHC stains. Stain1 is 
done on both blocks A1 and A2.  Stain2 is done on block A1.
How would we bill for this?  Would it be 88344X2 - because there are two 
distinct cocktails. OR would it be 88344X1 - because you can only charge one 
per container?

Thanks in advance,
Michelle

Sent from my iPhone
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[Histonet] Anyone using Biogenex stainers

[Morken, Timothy]

Does anyone use a Biogenex stainer these days? They used to be a premier 
company but seem to have disappeared over the last decade.  They still display 
at NSH and have ads in various places but am not aware of any lab that uses one.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
Box 1656
505 Parnassus Ave
San Francisco, CA 94143
USA

415.514-6042  (office)
tim.mor...@ucsfmedctr.org

CONFIDENTIALITY NOTICE: This email message, including any attachments, is for 
the sole use of the intended recipient(s) and may contain confidential, 
proprietary, and/or privileged information protected by law. If you are not the 
intended recipient, you may not use, copy, or distribute this email message or 
its attachments. If you believe you have received this email message in error, 
please contact the sender by reply email and destroy all copies of the original 
message.



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Re: [Histonet] X-gal staining

[koellingr]

Kristopher, 
beta-gal is an enzyme and as far as I know is rendered inert after FFPE.  I did 
do fresh skin in X-gal to target, as you would with any whole mount specimen, 
THEN FFPE and cut sections to it to see the signal.  If that is not an option, 
go after it with an anti-beta galactosidase antibody (polyclonal-like Pierce I 
used-have zero relationship to them) from several places.  Make sure the 
immunogen is a full, length protein that produced the antibody, and it will go 
much easier. 
  
Ray 
  
Raymond Koelling 
Seattle, WA area 

- Original Message -

From: "Kristopher Kalleberg"  
To: histonet-requ...@lists.utsouthwestern.edu, 
histonet@lists.utsouthwestern.edu 
Sent: Thursday, January 8, 2015 9:53:38 AM 
Subject: [Histonet] X-gal staining 

Hello All, 

Does anyone know of a commercially available X-gal stain that would work on 
formalin fixed paraffin embedded skin samples in order to detect beta 
galactosidase.  Thank you in advance. 

Kris 
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[Histonet] X-gal staining

[Kalleberg, Kristopher]

Hello All,

Does anyone know of a commercially available X-gal stain that would work on 
formalin fixed paraffin embedded skin samples in order to detect beta 
galactosidase.  Thank you in advance.

Kris
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Re: [Histonet] Celestin Blue B

[Hans B Snyder]

Hello,

Just in case you didn't find this yet, Rowley Biochemical in Danvers
MA sells this.

http://www.rowleybio.com/order.php

Good luck!
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
h...@histologistics.com


On Tue, Jan 6, 2015 at 5:18 PM, John Smallwood  wrote:
> Hello Histologists:
> Is  there anywhere to get this ?  Celestin Blue B C.I. 
> 51050
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[Histonet] Histology Supervisor Job Opening in Buffalo, NY

[Melissa Owens]

Hello and Happy New Year. My firm has a histology supervisor opening in 
Buffalo, NY. It requires at least a year of histology supervisor or lead or 
senior experience within a clinical histology department and a new york 
clinical lab or histology license. Please contact me if you are interested and 
I can give you more details. Thank you,

Melissa Owens (Phelan)
President, Laboratory Staffing
Allied Search Partners
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[Histonet] New 88344 CPT code question

[histot...@imagesbyhopper.com]

Hello Histonetters :)

With regards to billing for multiplex IHC stains, I have a scenario and 
question.
Scenario: 1 container, 2 blocks, 2 different cocktail IHC stains. Stain1 is 
done on both blocks A1 and A2.  Stain2 is done on block A1.
How would we bill for this?  Would it be 88344X2 - because there are two 
distinct cocktails. OR would it be 88344X1 - because you can only charge one 
per container?

Thanks in advance,
Michelle

Sent from my iPhone
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[Histonet] RE: Slide and cassette labelers

[Blazek, Linda]

You might also look at the Primera printers sold by Creative Waste Solutions.  
I have both the slide and cassette printer and like them both.  They are nice 
and compact.

http://cwsincorp.com/

Linda
Linda Blazek HT (ASCP)
Manager 
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James
Sent: Thursday, January 08, 2015 9:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide and cassette labelers


Has anyone used the Tissue Tex "SmartWrite" Slide Printer and Cassette Printer? 
  These both use Thermal transfer.

I am also considering ThermoScientific, Leica, and General Data.

Any advice would be welcome.

Thanks

Jim



Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com


This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
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[Histonet] RE: Research cases

[Goins, Tresa]

We charge by the hour.  The condition of the samples when received varies 
greatly (container, sample ID, master list, etc.) and by the hour rewards those 
who are organized and charges a fair rate for those who are not so organized. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Thursday, January 08, 2015 7:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Research cases

Good morning everyone!

I wanted to ask what others are doing about charges when blocks & slides are 
being requested for research purposes. We have been asked to provide a cost for 
this service, because the research companies are willing to pay the lab for our 
time processing, embedding, and cutting slides specifically for this purpose. 
Any guidance in this would be greatly appreciated!


Toni Rathborne
Pathology Supervisor

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Re: [Histonet] Research cases

[Rene J Buesa]

You should know your own costs for the whole process and you should add a 
"fair" amount as an external service.Talk with your lab manager and get to a 
consensus on how to proceed but your costs have to be your essential guide.René 
J.  

 On Thursday, January 8, 2015 9:45 AM, "Rathborne, Toni" 
 wrote:
   

 Good morning everyone!

I wanted to ask what others are doing about charges when blocks & slides are 
being requested for research purposes. We have been asked to provide a cost for 
this service, because the research companies are willing to pay the lab for our 
time processing, embedding, and cutting slides specifically for this purpose. 
Any guidance in this would be greatly appreciated!


Toni Rathborne
Pathology Supervisor
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[Histonet] Slide and cassette labelers

[Vickroy, James]

Has anyone used the Tissue Tex "SmartWrite" Slide Printer and Cassette Printer? 
  These both use Thermal transfer.

I am also considering ThermoScientific, Leica, and General Data.

Any advice would be welcome.

Thanks

Jim



Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com


This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
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[Histonet] Research cases

[Rathborne, Toni]

Good morning everyone!

I wanted to ask what others are doing about charges when blocks & slides are 
being requested for research purposes. We have been asked to provide a cost for 
this service, because the research companies are willing to pay the lab for our 
time processing, embedding, and cutting slides specifically for this purpose. 
Any guidance in this would be greatly appreciated!


Toni Rathborne
Pathology Supervisor
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RE: [Histonet] ASP300S or VIP 6

[ian bernard]

Rachel, thanks for the question.  For us, our reagent cost is comparable
other than now we have another processor so will have to acquire more
reagents to support both since we plan on running them both daily.  However,
another feature with the ASP 300 is that owing to the Reagent Management
System our reagents can last 3 times longer than reagent we used on the
VIP-5, per our protocol.  It also has a paraffin cleaning system. Where it
removes residue clearing agent from the paraffin thus preserving the life of
the paraffin as well.

And, in case all are wondering, I am not a spoke person for Leica or the
ASP300S. Just a happy and practical customer with his new equipment.

IRB

-Original Message-
From: Rachel Gonzalez [mailto:rac...@gbi-inc.com] 
Sent: Wednesday, January 07, 2015 7:37 PM
To: 'ian bernard'
Subject: RE: [Histonet] ASP300S or VIP 6

Hi 

Are  the reagent cost comparable. 

Thanks
Rachel

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ian bernard
Sent: Wednesday, January 7, 2015 5:05 PM
To: 'Vickroy, James'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] ASP300S or VIP 6

James, we have both the VIP-5 and Leica ASP300S.  While the VIP has
sustained us well through the years, notwithstanding, with malfunctions
since it's on its last leg; the ASP 300S was our researched replacement
and/or alternate processor of choice.  Since our validations and use of both
processors in parallel , we are happy to have made the transition to the
ASP-300S. 

It is user friendly, with nice graphics,  and with the operating manual on
the screen and a niche reagent management system as well as the auto fill
feature are excellent options to have.

IRB 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy,
James
Sent: Wednesday, January 07, 2015 11:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ASP300S or VIP 6


Buying two automated tissue processors for new lab.   I have always used the
VIP tissue processors, can anyone comment on a side by side comparison
between the Leica and the VIP 6?

thanks

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:
jvick...@springfieldclinic.com


This electronic message contains information from Springfield Clinic, LLP
that may be confidential, privileged, and/or sensitive. This information is
intended for the use of the individual(s) or entity(ies) named above. If you
are not the intended recipient, be aware that disclosure, copying,
distribution, or action taken on the contents of this information is
strictly prohibited. If you have received this electronic message in error,
please notify the sender immediately, by electronic mail, so that
arrangements may be made for the retrieval of this electronic message. Thank
you.
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