[Histonet] Alk pho staining on paraffin section

[abtdhu]

Hi,

I couldn't do on paraffin tissue after trying a few times. But works fine on 
MMA plastic section.
If any one has a practical protocol, please share. Thanks in advance.

Dorothy Hu


Hello,



I was wondering if anyone has had success in staining osteoblasts via
alkaline phosphatase in decaled ffpe tissues? Currently using a sigma kit
without success even with pre-incubating overnight with magnesium chloride.

Thanks!
Melanie

-- 
Melanie Smith, MS
University of Delaware
melsm...@udel.edu
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RE: [Histonet] Masson trichrome and H and E

[Hugh Luk]

Hi Emily,

My $0. 02 by saying it's a challenge for research groups, like ours, to keep 
quality stains that are not used everyday/week/month.  

I agree with your intuition.  Buy or make new eosin.

As for stocking eosin (or hematoxylin), I buy premade by a reputable vendor and 
always keep an eye on it getting weak or the dye adhering to the bottle.  
Oxidation hurts H&E stains, so I never return used dye to the "New" bottle once 
I have used them (I get rather oblique requests too).  Alcohols are similar, as 
they always pull water out of the air, so my final dehydrating alcohols are 
always fresh.  Xylenes are less fussy.

For manual Masson trichrome, I use Newcomer Supply as I appreciate Marcia 
Walsh's humor, but most vendors are pretty good.  250 ml of aniline blue is 
$28.90.  Works great.  I don't know why your kit was $800, but I would guess it 
would be the phosphomolybdic/phoshotungstic acid.  Her Masson kit (blue) is 
rather high too at $294 for the 250 ml.

For me, aniline blue solution crashes first in the trichrome (other than the 
Weigerts).  Have you any desire to make solution from powder?  $27.10 for 25 g 
of certified dye?  Just wondering.  Shipping to my state adds $50-$100 to the 
price, so I think about stuff like this.

Hugh
Hawaii


> --
> Date: Tue, 3 Mar 2015 07:38:30 -0500
> From: Emily Brown 
> Subject: [Histonet] Masson trichrome and H and E
> To: "histonet@lists.utsouthwestern.edu"
>   
> Message-ID:
>   
> Content-Type: text/plain; charset=UTF-8
> 
> Hello!
> 
> I am doing Masson trichrome manually (not with a machine) and I just found
> out the kit is $800!! I was thinking of buying the ingredients separately,
> but why the hell is it so expensive???
> Also, the people I was going to borrow their reagents from said their
> aniline blue is not very good and I wanted to replace it.  I only need
> about 250ml, what brand do you guys prefer?
> l know I can google this, but I want to know what you guys like and what
> works best.  This is for mouse kidney paraffin sections, 4 to 5 microns.
> Another question, I did H and E and there is no eosin staining.  I think
> the reagents are pretty old, so I thought that might be a problem.  Also
> because my lab is cheap, they were reusing the xylenes and EtOH for both
> rehydrating and rehydrating.  I told my boss this is probably not a good
> idea as the end steps will have stain in them.  And I also think this is
> why it didn't work! The EtOH is also really old so who know is the 100% is
> actually even close to 100% any more.  I'm buying new reagents, but if you
> guys think anything else would help, let me know.
> 
> Also, shoutout to Ann, I know you're reading these!! Join the list!!
> 
> Emily
> 
> --

  
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[Histonet] Differentiating mycobacteria from Nocardia

[Conway, Carla]

Hello,

Could anyone provide a staining protocol that will differentiate
mycobacteria from Nocardia in tissue sections? The bacteria were both
acid-fast-positive by the Ziehl-Neelsen stain.

Thank you!

Carla




Carla Conway
Histology Technician
Western Fisheries Research Center, USGS
6505 N.E. 65th Street
Seattle, WA 98115-5016 USA
Phone: 206-526-2042
Fax: 206-526-6654
E-mail: cmcon...@usgs.gov
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[Histonet] Microscopy training courses or certification programs

[Coffey, Anna (NIH/NCI) [C]]

Hello Histonet,

I am curious if anyone can recommend a good microscopy training course or 
certificate program? I'm looking primarily for training on light microscopes.

Thanks,
Anna

Anna Coffey, MS, HTL(ASCP)CM
Histotechnologist
Center for Advanced Preclinical Research
Frederick National Laboratory for Cancer Research
Leidos Biomedical Research, Inc.
Bld 539, 224
Frederick, Maryland 21702
301-846-1730

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[Histonet] Frozen section calculations

[Algeo, Lacie A]

Hi All,
When you receive multiple frozen sections on the same patient, does the 
pathologist call each one and then document the time for each one, or do they 
call in 'batches' and the time from receipt to call is divided by the total 
number of frozens done in that time frame for that patient.  Or, does anyone 
have a different way that they do it?  Is there any documentation recommending 
the process or recording frozen section times?
Thanks,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.al...@providence.org


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may not use, copy, disclose or distribute to anyone the message or any 
information contained in the message.  If you have received this message in 
error, please immediately advise the sender by reply e-mail and delete this 
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may not use, copy, disclose, or distribute to anyone the message or any 
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error, please immediately advise the sender by reply email and delete this 
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[Histonet] Dako Coverstainer

[Sanders, Jeanine (CDC/OID/NCEZID)]

Good afternoon all!

I would like to hear back from those of you who have the Dako 
Coverstainerpros and cons!

Thanks much!


Jeanine H. Sanders
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-639-3590
j...@cdc.gov


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[Histonet] Histology Supervisor and Bench histotech opportunities in Alpharetta, Ga.

[Taylor Rinaldi]

A growing Pathology laboratory in Alpharetta, Ga is looking to bring some
fresh faces to their Histology department. They are currently looking to
hire a new Histology Supervisor, and a bench Histotechnician. ASCP
certification preferred. This is a permanent, full time opportunity. 

 

Please reach out to me for immediate consideration.

 

Thanks so much!

 

Taylor Rinaldi

Nationwide Laboratory Recruiter

Prometheus Healthcare 

Office (301) 693-9057

tay...@prometheushealthcare.com

 

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Re: AW: [Histonet] Masson trichrome and H and E

[Rene J Buesa]

Emely:
To your initial e-mail the kit is so expensive because it incorporates a salary 
component for the preparation of the solutions + any and all enormous profit 
margin the manufacturer wants to add.I never/ever used kits, always used 
solutions prepared "from scratch" in my lab and the savings were enormous.As to 
preferred dry dyes my best preferred are Merk (Darmstad), Harleco and 
Sigma.René J.  

 On Tuesday, March 3, 2015 1:19 PM, Gudrun Lang  wrote:
   

 Hi!
For lacking Eosin-staining check the pH of the solution. It should be 4-5.
With some drops of acetic acid you may recover the staining-action.
Gudrun
= = =
On 03/03/15, Emily Brown   wrote:
> Hello!
> 
> I am doing Masson trichrome manually (not with a machine) and I just 
> found out the kit is $800!! I was thinking of buying the ingredients 
> separately, but why the hell is it so expensive???
> Also, the people I was going to borrow their reagents from said their 
> aniline blue is not very good and I wanted to replace it. I only need 
> about 250ml, what brand do you guys prefer?
> l know I can google this, but I want to know what you guys like and 
> what works best. This is for mouse kidney paraffin sections, 4 to 5
microns.
> Another question, I did H and E and there is no eosin staining. I 
> think the reagents are pretty old, so I thought that might be a 
> problem. Also because my lab is cheap, they were reusing the xylenes 
> and EtOH for both rehydrating and rehydrating. I told my boss this is 
> probably not a good idea as the end steps will have stain in them. And 
> I also think this is why it didn't work! The EtOH is also really old 
> so who know is the 100% is actually even close to 100% any more. I'm 
> buying new reagents, but if you guys think anything else would help, let
me know.
> 
> Also, shoutout to Ann, I know you're reading these!! Join the list!!
> 
> Emily
> 
> 
> "By bitching and bitching and bitching, they could exhaust the drama 
> of their own horror stories. Grow bored. Only then could they accept a 
> new story for their lives. Move forward."
> 
> -Chuck Palahniuk, "Haunted"
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
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Re: [Histonet] IHC work flow

[Rene J Buesa]

Clare:
I think that in order to benefit the most from potential answers, you should 
have first offered the information from your lab for each of the questions, 
along with your TAT.By doing so those willing to offer their information on the 
subject would have a better idea if your lab is performing at par with theirs.I 
think that in this way you will benefit the most.René J.  

 On Tuesday, March 3, 2015 1:09 PM, Clare Thornton 
 wrote:
   

 I would love to hear about your IHC department or lab.  We are looking to 
changing our work flow, and sometimes it's difficult to know if you are trying 
to perform miracles!  These are the questions I have:


1.      What is your volume of IHC slides/month?

2.      How many antibodies do you offer?

3.      What platforms do you use?  Do you use platforms from multiple vendors?

4.      What is your turn around time?  Do you offer same day turn around, and 
under what conditions?

5.      How many full time employees work in IHC?  Do any work on a part time 
or rotating basis?

6.      Is your IHC department part of general histology, or is it a separate 
department?

7.      If you are continuous flow, how does this work?  I will say that we use 
Ventana platforms (both Ultra and XT), so I would be interested in hearing 
about those who also use Ventana, but I would like to hear from users with 
other platforms as well.

8.      If there are any other aspects you would like to mention, please add 
them!


I appreciate the help on this.  Every lab is a world unto itself, often seeing 
how other labs run their operations can open your eyes to additional 
possibilities.  Thank you.

Clare J. Thornton, HTL(ASCP)QIHC
Lead Immunohistochemistry Technologist
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com


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AW: [Histonet] Masson trichrome and H and E

[Gudrun Lang]

Hi!
For lacking Eosin-staining check the pH of the solution. It should be 4-5.
With some drops of acetic acid you may recover the staining-action.
Gudrun
= = =
On 03/03/15, Emily Brown   wrote:
> Hello!
> 
> I am doing Masson trichrome manually (not with a machine) and I just 
> found out the kit is $800!! I was thinking of buying the ingredients 
> separately, but why the hell is it so expensive???
> Also, the people I was going to borrow their reagents from said their 
> aniline blue is not very good and I wanted to replace it. I only need 
> about 250ml, what brand do you guys prefer?
> l know I can google this, but I want to know what you guys like and 
> what works best. This is for mouse kidney paraffin sections, 4 to 5
microns.
> Another question, I did H and E and there is no eosin staining. I 
> think the reagents are pretty old, so I thought that might be a 
> problem. Also because my lab is cheap, they were reusing the xylenes 
> and EtOH for both rehydrating and rehydrating. I told my boss this is 
> probably not a good idea as the end steps will have stain in them. And 
> I also think this is why it didn't work! The EtOH is also really old 
> so who know is the 100% is actually even close to 100% any more. I'm 
> buying new reagents, but if you guys think anything else would help, let
me know.
> 
> Also, shoutout to Ann, I know you're reading these!! Join the list!!
> 
> Emily
> 
> 
> "By bitching and bitching and bitching, they could exhaust the drama 
> of their own horror stories. Grow bored. Only then could they accept a 
> new story for their lives. Move forward."
> 
> -Chuck Palahniuk, "Haunted"
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
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[Histonet] IHC work flow

[Clare Thornton]

I would love to hear about your IHC department or lab.  We are looking to 
changing our work flow, and sometimes it's difficult to know if you are trying 
to perform miracles!  These are the questions I have:


1.   What is your volume of IHC slides/month?

2.   How many antibodies do you offer?

3.   What platforms do you use?  Do you use platforms from multiple vendors?

4.   What is your turn around time?  Do you offer same day turn around, and 
under what conditions?

5.   How many full time employees work in IHC?  Do any work on a part time 
or rotating basis?

6.   Is your IHC department part of general histology, or is it a separate 
department?

7.   If you are continuous flow, how does this work?  I will say that we 
use Ventana platforms (both Ultra and XT), so I would be interested in hearing 
about those who also use Ventana, but I would like to hear from users with 
other platforms as well.

8.   If there are any other aspects you would like to mention, please add 
them!


I appreciate the help on this.  Every lab is a world unto itself, often seeing 
how other labs run their operations can open your eyes to additional 
possibilities.  Thank you.

Clare J. Thornton, HTL(ASCP)QIHC
Lead Immunohistochemistry Technologist
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com


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Re: [Histonet] Masson trichrome and H and E

[John Kiernan]

If you can't get two colours with H&E, don't expect to get the colour scheme 
right with Masson's trichrome, which needs more skill. If you are hoping to 
show basement membranes in the kidney, you would do better to use a technically 
simpler staining method such as picro-sirius red or periodic acid-Schiff. If 
for some reason you really need three colours, a one-step trichrome such as 
Gomori's, Cason's or Gabe's might be the way to go rather than Masson's or one 
of the other multi-step trichromes. Remember that all trichrome methods are 
greatly influenced by the fixative. A post-fixation treatment of the sections, 
usually with picric acid, is needed for formaldehyde-fixed tissues. Some 
alternative post-fixation treatments were proposed by Yu & Chapman (2003) J. 
Histotechnol. 26(2): 131-134, but their coloured photos were not very 
convincing.

Making up staining solutions in-house is always cheaper than buying pre-made 
solutions.

John Kiernan
London, Canada 
= = =
On 03/03/15, Emily Brown   wrote:
> Hello!
> 
> I am doing Masson trichrome manually (not with a machine) and I just found
> out the kit is $800!! I was thinking of buying the ingredients separately,
> but why the hell is it so expensive???
> Also, the people I was going to borrow their reagents from said their
> aniline blue is not very good and I wanted to replace it. I only need
> about 250ml, what brand do you guys prefer?
> l know I can google this, but I want to know what you guys like and what
> works best. This is for mouse kidney paraffin sections, 4 to 5 microns.
> Another question, I did H and E and there is no eosin staining. I think
> the reagents are pretty old, so I thought that might be a problem. Also
> because my lab is cheap, they were reusing the xylenes and EtOH for both
> rehydrating and rehydrating. I told my boss this is probably not a good
> idea as the end steps will have stain in them. And I also think this is
> why it didn't work! The EtOH is also really old so who know is the 100% is
> actually even close to 100% any more. I'm buying new reagents, but if you
> guys think anything else would help, let me know.
> 
> Also, shoutout to Ann, I know you're reading these!! Join the list!!
> 
> Emily
> 
> 
> "By bitching and bitching and bitching, they could exhaust the drama of
> their own horror stories. Grow bored. Only then could they accept a new
> story for their lives. Move forward."
> 
> -Chuck Palahniuk, "Haunted"
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
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[Histonet] HT vacancy

[Houston, Ronald]

We have an immediate opening for a  registered HT. This would be a great 
opening for a newly registered tech as you would have the opportunity to learn 
and perform all aspects of histotechnology, from routine microtomy to electron 
microscopy, from special stains to IHC/ISH/IF and enzyme histochemistry.

Please contact me for more information and go to our web-site to apply 
(https://careers.nationwidechildrens.org) and to see our incredible new 
facility (http://www.nationwidechildrens.org/)


Come work for an organization where everything matters


Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster

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RE: [Histonet] Masson trichrome and H and E

[Wineman, Terra]

I use the kit from Sigma-Aldrich (HT15 = $300).


Terra Wineman, HTL (ASCP)CM
Research Biologist, Nutritional Physiology
636-926-7476 phone
terra.wine...@novusint.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Tuesday, March 03, 2015 9:46 AM
To: Emily Brown; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Masson trichrome and H and E

Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around 
$60.00.

Laurie Colbert, HT (ASCP)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Tuesday, March 03, 2015 4:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Masson trichrome and H and E

Hello!

I am doing Masson trichrome manually (not with a machine) and I just found out 
the kit is $800!! I was thinking of buying the ingredients separately, but why 
the hell is it so expensive???
Also, the people I was going to borrow their reagents from said their aniline 
blue is not very good and I wanted to replace it.  I only need about 250ml, 
what brand do you guys prefer?
l know I can google this, but I want to know what you guys like and what works 
best.  This is for mouse kidney paraffin sections, 4 to 5 microns.
Another question, I did H and E and there is no eosin staining.  I think the 
reagents are pretty old, so I thought that might be a problem.  Also because my 
lab is cheap, they were reusing the xylenes and EtOH for both rehydrating and 
rehydrating.  I told my boss this is probably not a good idea as the end steps 
will have stain in them.  And I also think this is why it didn't work! The EtOH 
is also really old so who know is the 100% is actually even close to 100% any 
more.  I'm buying new reagents, but if you guys think anything else would help, 
let me know.

Also, shoutout to Ann, I know you're reading these!! Join the list!!

Emily


"By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward."

-Chuck Palahniuk, "Haunted"
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[Histonet] New York State Histotechnological Society Annual Meeting

[Mack, Sarah]

The 2015 NYSHS Annual Meeting will be held on Saturday the 18th of April, 2015 
at the Holiday Inn Express & Suites Latham in Latham New York. 
Check out our website for more information. http://www.nyhisto.com/

Sarah Mack

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[Histonet] Lynch syndrome

[Houston, Ronald]

I know it is a long-shot, but does anyone know of a TMA made with known Lynch 
Syndrome cases?

We have been asked to validate MSI antibodies for evaluating CMMRD 
(Constitutional Mismatch Repair Deficiency) in pediatric brain tumors and don't 
have access to Lynch Syndrome in our pediatric facility

Any help appreciated

Thanks
Ronnie

Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com

700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.hous...@nationwidechildrens.org
www.NationwideChildrens.org

"One person with passion is better than forty people merely interested."
~ E.M. Forster

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RE: [Histonet] Masson trichrome and H and E

[Laurie Colbert]

Medical Chemical Corporation has a trichrome kit with 4 oz reagents for around 
$60.00.

Laurie Colbert, HT (ASCP)


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Tuesday, March 03, 2015 4:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Masson trichrome and H and E

Hello!

I am doing Masson trichrome manually (not with a machine) and I just found out 
the kit is $800!! I was thinking of buying the ingredients separately, but why 
the hell is it so expensive???
Also, the people I was going to borrow their reagents from said their aniline 
blue is not very good and I wanted to replace it.  I only need about 250ml, 
what brand do you guys prefer?
l know I can google this, but I want to know what you guys like and what works 
best.  This is for mouse kidney paraffin sections, 4 to 5 microns.
Another question, I did H and E and there is no eosin staining.  I think the 
reagents are pretty old, so I thought that might be a problem.  Also because my 
lab is cheap, they were reusing the xylenes and EtOH for both rehydrating and 
rehydrating.  I told my boss this is probably not a good idea as the end steps 
will have stain in them.  And I also think this is why it didn't work! The EtOH 
is also really old so who know is the 100% is actually even close to 100% any 
more.  I'm buying new reagents, but if you guys think anything else would help, 
let me know.

Also, shoutout to Ann, I know you're reading these!! Join the list!!

Emily


"By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward."

-Chuck Palahniuk, "Haunted"
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[Histonet] Histotech coverage needed in Milwaukee, WI

[Matthew Fleming]

Help needed in covering a histotech's vacation in dermatopathology lab in
downtown Milwaukee, WI. We need about 4 weeks' of coverage per year but one
tech would not have to do all of it; even a week or 2 would be helpful. If
interested, please respond to:

Matthew Fleming, MD
Fleming Dermatopathology
1661 N Water St, Ste 500
Milwaukee, WI 53202
mgf...@gmail.com
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RE: [Histonet] Masson trichrome and H and E

[Rathborne, Toni]

We have been very satisfied with special stain solutions from Poly Scientific 
(http://www.polyrnd.com/products/stains/general/aniline-blue---masson's-trichrome.aspx).
 We get an 8oz bottle for under $30. Very good customer service too.

Toni

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Brown
Sent: Tuesday, March 03, 2015 7:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Masson trichrome and H and E

Hello!

I am doing Masson trichrome manually (not with a machine) and I just found out 
the kit is $800!! I was thinking of buying the ingredients separately, but why 
the hell is it so expensive???
Also, the people I was going to borrow their reagents from said their aniline 
blue is not very good and I wanted to replace it.  I only need about 250ml, 
what brand do you guys prefer?
l know I can google this, but I want to know what you guys like and what works 
best.  This is for mouse kidney paraffin sections, 4 to 5 microns.
Another question, I did H and E and there is no eosin staining.  I think the 
reagents are pretty old, so I thought that might be a problem.  Also because my 
lab is cheap, they were reusing the xylenes and EtOH for both rehydrating and 
rehydrating.  I told my boss this is probably not a good idea as the end steps 
will have stain in them.  And I also think this is why it didn't work! The EtOH 
is also really old so who know is the 100% is actually even close to 100% any 
more.  I'm buying new reagents, but if you guys think anything else would help, 
let me know.

Also, shoutout to Ann, I know you're reading these!! Join the list!!

Emily


"By bitching and bitching and bitching, they could exhaust the drama of their 
own horror stories. Grow bored. Only then could they accept a new story for 
their lives. Move forward."

-Chuck Palahniuk, "Haunted"
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[Histonet] Masson trichrome and H and E

[Emily Brown]

Hello!

I am doing Masson trichrome manually (not with a machine) and I just found
out the kit is $800!! I was thinking of buying the ingredients separately,
but why the hell is it so expensive???
Also, the people I was going to borrow their reagents from said their
aniline blue is not very good and I wanted to replace it.  I only need
about 250ml, what brand do you guys prefer?
l know I can google this, but I want to know what you guys like and what
works best.  This is for mouse kidney paraffin sections, 4 to 5 microns.
Another question, I did H and E and there is no eosin staining.  I think
the reagents are pretty old, so I thought that might be a problem.  Also
because my lab is cheap, they were reusing the xylenes and EtOH for both
rehydrating and rehydrating.  I told my boss this is probably not a good
idea as the end steps will have stain in them.  And I also think this is
why it didn't work! The EtOH is also really old so who know is the 100% is
actually even close to 100% any more.  I'm buying new reagents, but if you
guys think anything else would help, let me know.

Also, shoutout to Ann, I know you're reading these!! Join the list!!

Emily


"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"
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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet