[Histonet] CSH Symposium
The California Society for Histotechnology will be hosting their 39th Annual Symposium at the Hilton Scotts Valley/Santa Cruz May 1-3. The link below will take you to our website. CE certificates will be issued at the completion of each workshop for those that have pre-registered for the event. The program can also be emailed to you if you contact me. Jennifer http://www.californiahistology.org/events.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Mushrooms for GMS fungus control
Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to . Sounds like fun to me. I'm curious, with the emphasis now on quality control in labs run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls? A PI in research who doesn't want his paper rejected at peer review. A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency. The dot-your-i's and cross-your-t's FDA people who might or might not OK your drug in development. Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. Ray, Seattle, WA - Original Message - From: "Linda Prasad (SCHN)" To: "Jeffrey Robinson" , histonet@lists.utsouthwestern.edu Sent: Sunday, March 8, 2015 4:09:02 PM Subject: [Histonet] RE: Mushrooms for GMS fungus control I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ♲ Please consider the environment before printing this email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Mushrooms for GMS fungus control
I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ♲ Please consider the environment before printing this email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FW: Masson's trichrome stain
Here is a message from Justine... From: Justine Lanzon [mailto:justinelan...@hotmail.com] Sent: Thursday, March 05, 2015 5:36 AM To: lindamarg...@gmail.com Subject: Masson's trichrome stain Hi, I am doing a write up on Masson's trichrome stain however I cannot answer these two questions: - Why are plastic forceps used instead of metal ones to hold the stained slide? - Why do we not rinse before Alinine blue? Can you please help me? Many Thanks, Justine Lanzon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Stain vs dye and control
There is always confusion about this. In textile "coloring" the term dyeing is used and this first carried over to histology as many of the textile dyes were the ones first used to stain tissue components. Some of these were specific and some non specific. In histology my understanding is that the dye is the powder, while the stain is the solution used for staining the tissues. and in most cases there is a certain amount of specificity in the reaction. Merely imparting a non specific color all components of a tissue I would regard as coloring as there is no specificity in this process. This raised the interesting question of what would you call the process whereby osmium tetroxide is used to fix and stain some fats? To further complicate in the concrete industry the term dye is used to indicate a general coloring of the concrete while the term stain is used to indicate a chemical reaction with the concrete components. Barry On Sun, Mar 8, 2015 at 3:02 AM, Gudrun Lang wrote: > Hi Jorge, > Not all histolabs have access to fresh cultures. So they search an easy > way to get bacterial controls. And things like sausage are more like the > usual specimens than liquid samples. > > The best staining control is an inhouse-specimen, that is processed in the > same way as any other specimen (preanalytic, fixation, processing, cutting, > staining). But sometimes this is not possible, so one uses the "next-best". > A control with known ingredients (like bacteria) can be used to check the > whole process and must be positive for the tested parameter. A > patient-sample can only be considered positive or negative, if the > positive-control proves the functionality of the process > (staining-protocol). > > Dye and stain. You can touch the dye, but not the stain. And then you > have got stained fingers. ;-) > > Gudrun > > > > -Ursprüngliche Nachricht- > Von: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Jorge A. > Santiago-Blay > Gesendet: Freitag, 06. März 2015 21:49 > An: Histonet@lists.utsouthwestern.edu > Betreff: [Histonet] Stain vs dye and control > > Dear Histonetters: > > Last semester I taught a microbiology lab and, as I was reviewing for > class, noticed some lack of precision in the use of the terms "dye" vs. > "stain" in biology. Could someone help? > > While I am on "stains", I have been following the emails on controls and > wonder a couple of things. > > 1. What would testing for bacteria on beef jerky, hot dogs or burgers > accomplish that is different (ideally better) that what one accomplishes by > pulling out from fresh cultures out of a medium (e.g. liquid, such as > broth, solid, such as slabs, agar)? Is it the idea to test for bacteria in > an animal tissue? If so, would a solid medium (like someone mentioned > recently, such as agar) do? > > 2. An advantage of using fresh bacterial cultures of known Gram is that it > could be used to test whether the reagents are good enough. Last semester I > had the suspicion that one (or more) of our Gram reagents where not up to > par. > > If you have any feedback, please feel free to email me directly at > blayjo...@gmail.com . Thank you. > > Sincerely, > > Jorge > > Jorge A. Santiago-Blay, PhD > blaypublishers.com > http://blayjorge.wordpress.com/ > http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Stain vs dye and control
Hi Jorge, Not all histolabs have access to fresh cultures. So they search an easy way to get bacterial controls. And things like sausage are more like the usual specimens than liquid samples. The best staining control is an inhouse-specimen, that is processed in the same way as any other specimen (preanalytic, fixation, processing, cutting, staining). But sometimes this is not possible, so one uses the "next-best". A control with known ingredients (like bacteria) can be used to check the whole process and must be positive for the tested parameter. A patient-sample can only be considered positive or negative, if the positive-control proves the functionality of the process (staining-protocol). Dye and stain. You can touch the dye, but not the stain. And then you have got stained fingers. ;-) Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Jorge A. Santiago-Blay Gesendet: Freitag, 06. März 2015 21:49 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Stain vs dye and control Dear Histonetters: Last semester I taught a microbiology lab and, as I was reviewing for class, noticed some lack of precision in the use of the terms "dye" vs. "stain" in biology. Could someone help? While I am on "stains", I have been following the emails on controls and wonder a couple of things. 1. What would testing for bacteria on beef jerky, hot dogs or burgers accomplish that is different (ideally better) that what one accomplishes by pulling out from fresh cultures out of a medium (e.g. liquid, such as broth, solid, such as slabs, agar)? Is it the idea to test for bacteria in an animal tissue? If so, would a solid medium (like someone mentioned recently, such as agar) do? 2. An advantage of using fresh bacterial cultures of known Gram is that it could be used to test whether the reagents are good enough. Last semester I had the suspicion that one (or more) of our Gram reagents where not up to par. If you have any feedback, please feel free to email me directly at blayjo...@gmail.com . Thank you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
