Re: [Histonet] Post fixing IHC slides in formalin

[Barry Rittman]

There are always discussions about fixation but I have never seen comments
about using vapor fixation for post fixing or for fixing fresh frozen
specimens.
Vapor fixation is simple to use and does not require any solvent as it uses
the tissue fluids in the case of fresh frozen sections or the solution
remaining after IHC.
Especially useful for formaldehyde, alcohol,  acetone, osmium tetroxide etc.
Barry

On Sat, Mar 21, 2015 at 12:14 PM, Ann Specian  wrote:

> Does anyone post fix their IHC slides in formalin in an effort to try to
> reduce tissue loss?  If so, does anyone have a protocol for this that they
> have used and have seen good results?
>
>
> If you have any other suggestions which can help to reduce tissue loss
> during IHC staining, I would love to hear from you.
> Thanks,
> Ann
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[Histonet] Post fixing IHC slides in formalin

[Ann Specian]

Does anyone post fix their IHC slides in formalin in an effort to try to reduce 
tissue loss?  If so, does anyone have a protocol for this that they have used 
and have seen good results?


If you have any other suggestions which can help to reduce tissue loss during 
IHC staining, I would love to hear from you.
Thanks,
Ann
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Re: [Histonet] temporary histotech position

[Ronda Mire]

Hi Tasha,

I live in Frederick, MD and would be happy to fill in for you.  I am board 
registered HT with 20 years experience. I worked at Meritus Medical Lab for 22 
years and then at CVPath Institute.  I would be happy to meet with you or send 
my resume for you review.

 Kind regards,

Ronda Mire
240-217-9881
> On Mar 20, 2015, at 2:16 PM, Campbell, Tasha M.  wrote:
> 
> Hello everyone, I was wondering if there is anyone out there that does 
> temporary work in histo labs in the Frederick MD area.  I run a small GI lab 
> and I will be going on maternity leave in October and we would like someone 
> to fill in.  I know that there are temp agencies I can go through, but I 
> thought I would try leaving the middle man out first because we are a small 
> lab and are trying to save money and they can have more costs.  I would need 
> someone for 8 wks.  The tech would have to accession, gross the biopsies and 
> of course process, embed, cut and stain.  I also do warthin starry and ABPAS 
> by hand.  There are on average 30 blocks a day and 10 specials a day.  You do 
> your own thing. Go at your own pace.  Flexible time.  No one bothers you. 
> Just as long as the work gets done. It's really a walk in the park.
> 
> If there is anyone in the area that is willing to do this, please email or 
> call me!
> 
> Thanks!!
> 
> 
> 
> 
> 
> Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144 (w)
> 304-685-9307 (c)
> 
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