Re: [Histonet] RE: Nuclear "Artifact"

[Sue]

we do not use freeze spray in the lab at all. this issue has really gotten me 
bummed out since it is so sporadic. I am going to try to see if it is 
associated to one day in particular. it is one PA but multiple techs have 
identified the issue. 
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Re: [Histonet] RE: Nuclear "Artifact"

[mucram11]

We don't even allow freeze spray in the lab.  

Sent from my Verizon 4G LTE Smartphone


-- Original message--
From: Jennifer MacDonald
Date: Wed, Apr 22, 2015 5:34 PM
To: Arbaugh, Roberta;
Cc: histonet@lists.utsouthwestern.edu;Lisa 
Roy;histonet-boun...@lists.utsouthwestern.edu;'Morken, Timothy';
Subject:RE: [Histonet] RE: Nuclear "Artifact"

Our pathologists also complained about some of the GI biopsies looking 
burnt.  We tracked all of the problem cases back to one histotech.  The 
histotech was causing the "burn" artifact with excessive use of freezing 
spray. 



From:   "Arbaugh, Roberta" 
To: "'Morken, Timothy'" , Sue 
, Lisa Roy 
Cc: "histonet@lists.utsouthwestern.edu" 

Date:   04/22/2015 11:32 AM
Subject:RE: [Histonet] RE: Nuclear "Artifact"
Sent by:histonet-boun...@lists.utsouthwestern.edu



We have had the same problem. We come to the conclusion that it was water 
droplets. We had the problem when our humidity was high in the lab, or our 
weekend run. The changes we made where :
1. We no longer process over the weekend . We cannot count on our heating 
and cooling in our building.
2. We place a towel and a thick piece of cardboard on top of the processor 
lid.
3.We do not use the really fine mesh cassettes. We will use formalin 
soaked sponges or perm papers.
4. We do not over pack cassette basket.
We had our processor check every time we saw the artifact and they could 
never find a problem with the processor.
Hope this help, Roberta

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
Sent: Wednesday, April 22, 2015 11:16 AM
To: Sue; Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Nuclear "Artifact"

"One pathologist said it looks like the tissue has been cooked."  Which 
could also be drying artifact after bx, before formalin.

"The only issue is we can have two biopsies right next to one another in 
the basket one looks good and one looks bad. My director also thinks it is 
the processors."

Same processor but two results? Solving intermittent problems takes time 
to check variables - time almost no one wants to spend to check out every 
possibility. But if one variable is the same for both, and the result is 
different, then most likely it is a different variable causing the 
problem.

I think the other idea suggested of checking the handling of the tissue at 
the source of the biopsy is more likely to shed some light on this issue.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department 
of Pathology UC San Francisco Medical Center





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, April 21, 2015 4:55 PM
To: Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Nuclear "Artifact"

OMG we are experiencing the same issue. At first it was just GI and now we 
are seeing it on prostate. One pathologist said it looks like the tissue 
has been cooked. The only issue is we can have two biopsies right next to 
one another in the basket one looks good and one looks bad. My director 
also thinks it is the processors. I had Thermo out and they could find 
nothing. We changed out all the reagents and the biopsies were fine than 
two days later we had some bad ones. I know in July Fisher had a formalin 
recall associated to the mixture of buffer, water and formalin. We thought 
that might be it but it is now almost a year later and all the bad 
formalin should be gone. The histotechs say the tissue is crunchy and they 
are right. I am running a test tonight of a small needle biopsy that I 
made from a colon. I placed it is straight formaldehyde overnight and am 
processing it on our biopsy cycle tonight. My director also wanted us to 
only put three levels on our Thermo, but he wanted the middle level to 
have empty baskets. I stopped that today because I think the other issue 
is that the poor biopsies may be on the top level and as the reagents are 
used the level changes, and also due to displacement with the middle level 
being empty the reagent levels may not reach the top. We just do not have 
the manpower to inspect every reagent every day, we have 6 processor and 
it would take a tech all day. We actually take a digital picture when they 
come out of the processor. I want to check my problems cases tomorrow. We 
do not use sponges but the only other like was the PA who was wrapping the 
blue paper very tight around the tissue. I really do not think this is the 
issue though.. Any other insight would be greatly appreciated.

Susan T. Paturzo
TJUH
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RE: [Histonet] RE: Nuclear "Artifact"

[Jennifer MacDonald]

Our pathologists also complained about some of the GI biopsies looking 
burnt.  We tracked all of the problem cases back to one histotech.  The 
histotech was causing the "burn" artifact with excessive use of freezing 
spray. 



From:   "Arbaugh, Roberta" 
To: "'Morken, Timothy'" , Sue 
, Lisa Roy 
Cc: "histonet@lists.utsouthwestern.edu" 

Date:   04/22/2015 11:32 AM
Subject:RE: [Histonet] RE: Nuclear "Artifact"
Sent by:histonet-boun...@lists.utsouthwestern.edu



We have had the same problem. We come to the conclusion that it was water 
droplets. We had the problem when our humidity was high in the lab, or our 
weekend run. The changes we made where :
1. We no longer process over the weekend . We cannot count on our heating 
and cooling in our building.
2. We place a towel and a thick piece of cardboard on top of the processor 
lid.
3.We do not use the really fine mesh cassettes. We will use formalin 
soaked sponges or perm papers.
4. We do not over pack cassette basket.
We had our processor check every time we saw the artifact and they could 
never find a problem with the processor.
Hope this help, Roberta

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
Sent: Wednesday, April 22, 2015 11:16 AM
To: Sue; Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Nuclear "Artifact"

"One pathologist said it looks like the tissue has been cooked."  Which 
could also be drying artifact after bx, before formalin.

"The only issue is we can have two biopsies right next to one another in 
the basket one looks good and one looks bad. My director also thinks it is 
the processors."

Same processor but two results? Solving intermittent problems takes time 
to check variables - time almost no one wants to spend to check out every 
possibility. But if one variable is the same for both, and the result is 
different, then most likely it is a different variable causing the 
problem.

I think the other idea suggested of checking the handling of the tissue at 
the source of the biopsy is more likely to shed some light on this issue.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department 
of Pathology UC San Francisco Medical Center





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, April 21, 2015 4:55 PM
To: Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Nuclear "Artifact"

OMG we are experiencing the same issue. At first it was just GI and now we 
are seeing it on prostate. One pathologist said it looks like the tissue 
has been cooked. The only issue is we can have two biopsies right next to 
one another in the basket one looks good and one looks bad. My director 
also thinks it is the processors. I had Thermo out and they could find 
nothing. We changed out all the reagents and the biopsies were fine than 
two days later we had some bad ones. I know in July Fisher had a formalin 
recall associated to the mixture of buffer, water and formalin. We thought 
that might be it but it is now almost a year later and all the bad 
formalin should be gone. The histotechs say the tissue is crunchy and they 
are right. I am running a test tonight of a small needle biopsy that I 
made from a colon. I placed it is straight formaldehyde overnight and am 
processing it on our biopsy cycle tonight. My director also wanted us to 
only put three levels on our Thermo, but he wanted the middle level to 
have empty baskets. I stopped that today because I think the other issue 
is that the poor biopsies may be on the top level and as the reagents are 
used the level changes, and also due to displacement with the middle level 
being empty the reagent levels may not reach the top. We just do not have 
the manpower to inspect every reagent every day, we have 6 processor and 
it would take a tech all day. We actually take a digital picture when they 
come out of the processor. I want to check my problems cases tomorrow. We 
do not use sponges but the only other like was the PA who was wrapping the 
blue paper very tight around the tissue. I really do not think this is the 
issue though.. Any other insight would be greatly appreciated.

Susan T. Paturzo
TJUH
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Re: [Histonet] NY State regulations

[Garrey Faller]

Check this link out from CAP.
http://www.cap.org/apps/docs/education/lapaudio/pdf/031710_qa.pdf
Scroll down to #17. It address IHC.
Garrey

On Wed, Apr 22, 2015 at 4:32 PM, Joelle Weaver 
wrote:

> The FDA categorizes and grades each test based on the complexity of the
> test method. The FDA lists the category at
>
> *http://www.fda.gov/MedicalDevices/DeviceRegulationandGuidance/IVDRegulatoryAssistance/ucm393285.htm
> 
> *on the FDA website. The FDA categorizes test methods into three levels
> of complexity:
>
> Waived complexity, Moderate Complexity, including the subcategory of
> Provider-Performed Microscopy Procedures (PPMP); and High Complexity.
>
> When categorizing a test, the FDA considers the:
>
>
>
>
>1. Amount of interpretation involved;
>2. Calibration and quality control requirements of the instruments
>used;
>3. Degree of independent judgment involved;
>4. Difficulty of the calculations involved;
>5. Examinations and procedures performed and the methodologies
>employed; and
>6. Type of training required to operate the instruments used in the
>methodology.
>
>
> How is it determined if a test is waived, moderate or high complexity?
> For moderate and high complexity tests, the FDA evaluates each new
> commercial test system during the premarket approval process by scoring
> seven criteria as described in the CLIA regulations. The final score is
> used to determine whether the test system is classified as moderate or high
> complexity. See 42 CFR 493.17. For more details, please also see the FDA’s
> webpage on the CLIA Categorization Criteria
> [image:
> External Web Site Icon]  and
> CMS’ webpage on Categorization of Tests
> [image:
> External Web Site Icon] .
>
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>
>
>
>
>
> > From: caroline.pr...@uphs.upenn.edu
> > To: garr...@gmail.com; gmarce...@nj-urology.com
> > Date: Wed, 22 Apr 2015 19:33:35 +
> > Subject: RE: [Histonet] NY State regulations
> > CC: histonet@lists.utsouthwestern.edu
> >
> > Just a CLIA reg, but you are correct microtomy, embedding and routine
> stains are only Moderate Complexity testing.
> >
> > -Original Message-
> > From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garreyf
> > Sent: Wednesday, April 22, 2015 3:26 PM
> > To: Gail Marcella
> > Cc: histonet@lists.utsouthwestern.edu
> > Subject: Re: [Histonet] NY State regulations
> >
> > I believe grossing of small biopsies and performing ihc are both
> considered high complex testing. You must fulfill the clia personnel
> requirements of high complex testing.
> >
> > I also believe a histotech who only cuts and performs routine stains is
> not considered highly complex. I'm not sure why? Anyone know?
> >
> > Garrey
> >
> > Sent from my iPhone
> >
> > > On Apr 22, 2015, at 10:55 AM, Gail Marcella 
> wrote:
> > >
> > > Hi - I was wondering if anyone knows the regulations regarding the NY
> State Clinical Laboratory license. I have been a Histotech and have worked
> in IHC for 20+ years and was required to obtain a NY State Clinical Lab
> License in 2007. I don't have and associates or bachelor degree and was not
> required to prior to 2007. I was told on a job interview that if I don't
> have either of these degrees that I cannot gross any specimens or run IHC.
> I've never heard this before. Has anyone else ever heard of this??? Thanks
> - Gail
> > > ___
> > > Histonet mailing list
> > > Histonet@lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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[Histonet] Technovit 9100 new

[khong]

Hi,

it there anyone who has experience in Technovit 9100 new?
I'm having a hard time in polymerization process using T9100 now.
I just try to follow the manufacture instruction but it didn't work.

Pls give me an advice or suggestion.

Thanks,

Kai H



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[Histonet] Lifting sections off of slides

[Ann Specian]

Does anyone have a procedure where in you can lift the stained sections off of 
a slide and place them on another slide?



Sent from AOL Mobile Mail
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RE: [Histonet] NY State regulations

[Joelle Weaver]

The FDA categorizes and grades each test based on the complexity of the test 
method. The FDA lists the category at 
http://www.fda.gov/MedicalDevices/DeviceRegulationandGuidance/IVDRegulatoryAssistance/ucm393285.htm
 on the FDA website. The FDA categorizes test methods into three levels of 
complexity:


Waived complexity, Moderate Complexity, including the subcategory of 
Provider-Performed Microscopy Procedures (PPMP); and High Complexity.When 
categorizing a test, the FDA considers the:




Amount of interpretation involved;
Calibration and quality control requirements of the instruments used;
Degree of independent judgment involved;
Difficulty of the calculations involved;
Examinations and procedures performed and the methodologies employed; and
Type of training required to operate the instruments used in the methodology.
How is it determined if a test is waived, moderate or high complexity? 
For moderate and high complexity tests, the FDA evaluates each new commercial 
test system during the premarket approval process by scoring seven criteria as 
described in the CLIA regulations.The final score is used to determine 
whether the test system  is classified as moderate or high complexity. See 42 
CFR 493.17. For more details, please also see the FDA’s webpage on the CLIA 
Categorization Criteria and CMS’ webpage on Categorization of Tests.


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 > From: caroline.pr...@uphs.upenn.edu
> To: garr...@gmail.com; gmarce...@nj-urology.com
> Date: Wed, 22 Apr 2015 19:33:35 +
> Subject: RE: [Histonet] NY State regulations
> CC: histonet@lists.utsouthwestern.edu
> 
> Just a CLIA reg, but you are correct microtomy, embedding and routine stains 
> are only Moderate Complexity testing.
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garreyf
> Sent: Wednesday, April 22, 2015 3:26 PM
> To: Gail Marcella
> Cc: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] NY State regulations
> 
> I believe grossing of small biopsies and  performing ihc are both considered 
> high complex testing. You must fulfill the clia personnel requirements of 
> high complex testing.
> 
> I also believe a histotech who only cuts and performs routine stains is not 
> considered highly complex. I'm not sure why? Anyone know?
> 
> Garrey 
> 
> Sent from my iPhone
> 
> > On Apr 22, 2015, at 10:55 AM, Gail Marcella  
> > wrote:
> > 
> > Hi - I was wondering if anyone knows the regulations regarding the NY State 
> > Clinical Laboratory license. I have been a Histotech and have worked in IHC 
> > for 20+ years and was required to obtain a NY State Clinical Lab License in 
> > 2007. I don't have and associates or bachelor degree and was not required 
> > to prior to 2007. I was told on a job interview that if I don't have either 
> > of these degrees that I cannot gross any specimens or run IHC. I've never 
> > heard this before. Has anyone else ever heard of this??? Thanks - Gail
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
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> 
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Re: [Histonet] histology in higher education

[koellingr]

Hi, 
Thanks for info and happy to hear that.  Didn't realize.  May 10-15 is Worlds 
largest International Science and Engineering Fair in Pittsburgh this year and 
maybe will see others there and watch a high school histology project from 
somewhere bring home a $75,000 scholarship.  
  
Ray 

- Original Message -

From: "Joelle Weaver"  
To: koelli...@comcast.net, histonet@lists.utsouthwestern.edu 
Sent: Wednesday, April 22, 2015 11:53:14 AM 
Subject: RE: [Histonet] histology in higher education 

Many people do this, and have donated hundreds of hours of their own time. But 
definately not enough. Good to encourage people to get involved. 


Joelle Weaver MAOM, HTL (ASCP) QIHC 

 
   

  
> Date: Wed, 22 Apr 2015 14:07:18 + 
> From: koelli...@comcast.net 
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] histology in higher education 
> 
> The following has to do with histology and STEM (science, technology, 
> engineering, math) so if not interested, please ignore.  But I believe it can 
> have real meaning to the profession of histology at the NSH, state society 
> and local levels. 
>   
> I am elected to the Board of WSSEF (Washington State Science and Engineering 
> Fair) where I am in educational outreach and also the assistant to the head 
> judge.  We recently had our Washington State Fair with 650 kids, grades 1-12 
> from all over the state.  And while there was a lot of engineering and robots 
> and computers there were a few projects having to do with medicine, 
> biotechnology, immunology and pathology with some familiar histology or 
> immunohistochemistry pictures included.  At the end of the fair, we awarded 
> almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 
> students.  Not only that, our top winners get an all-expenses paid trip to 
> present at the ISEF (Intel International Fair) with 1,700 students competing 
> from all 50 states and 70 countries.  Wherever you are in the US, you have a 
> state fair. 
>   
> I would advocate for some of you so interested at the national, state or 
> local levels to promote histology, by getting involved as mentors for middle 
> and high school students to science fairs; especially those that could lead 
> to histopathology or other related projects that could lead into Intel 
> affiliated fairs resulting in great benefit to the student and a spread of 
> the word of histology into both the STEM world and general population. 
>   
> I've mentored for 15 years.  It can be done.  Molecular histopathology, 
> personalized diagnostics and therapeutics, advances in immunohistochemistry, 
> current controversies about breast biopsy diagnosis, or other disease with 
> newer classifications, PCR and RTPCR in histology, modern-targeted 
> therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image 
> analysis software for you computer geeks and on and on; the list is nearly 
> limitless.  Especially if you are close to or can contact biotech companies 
> or educational institutions to find co-mentors for grades 7-12 there are 
> histology-related science project possibilities in terms of data collection 
> and the scientific method and project presentation are nearly unlimited now. 
>   
> Be a mentor for or engage a grade 7-12 student, with the help of another 
> mentor or organization, to think about (histology-related) projects for 
> science fairs leading to a state fair and Intel ISEF.  Can't think of any 
> better way to "promote histology" so would hope those at NSH would take note 
> of this.  And since the ISEF fair receives projects and groups from 70 
> countries, I hope any outside the US would also think about the same thing. 
>   
> Ray Koelling 
> HT, HTL, QIHC, STEM educational outreach advocate 
> Lake Forest Park, WA 
> ___ 
> Histonet mailing list 
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet 

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RE: [Histonet] NY State regulations

[Pratt, Caroline]

Just a CLIA reg, but you are correct microtomy, embedding and routine stains 
are only Moderate Complexity testing.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Garreyf
Sent: Wednesday, April 22, 2015 3:26 PM
To: Gail Marcella
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] NY State regulations

I believe grossing of small biopsies and  performing ihc are both considered 
high complex testing. You must fulfill the clia personnel requirements of high 
complex testing.

I also believe a histotech who only cuts and performs routine stains is not 
considered highly complex. I'm not sure why? Anyone know?

Garrey 

Sent from my iPhone

> On Apr 22, 2015, at 10:55 AM, Gail Marcella  wrote:
> 
> Hi - I was wondering if anyone knows the regulations regarding the NY State 
> Clinical Laboratory license. I have been a Histotech and have worked in IHC 
> for 20+ years and was required to obtain a NY State Clinical Lab License in 
> 2007. I don't have and associates or bachelor degree and was not required to 
> prior to 2007. I was told on a job interview that if I don't have either of 
> these degrees that I cannot gross any specimens or run IHC. I've never heard 
> this before. Has anyone else ever heard of this??? Thanks - Gail
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] NY State regulations

[Garreyf]

I believe grossing of small biopsies and  performing ihc are both considered 
high complex testing. You must fulfill the clia personnel requirements of high 
complex testing.

I also believe a histotech who only cuts and performs routine stains is not 
considered highly complex. I'm not sure why? Anyone know?

Garrey 

Sent from my iPhone

> On Apr 22, 2015, at 10:55 AM, Gail Marcella  wrote:
> 
> Hi - I was wondering if anyone knows the regulations regarding the NY State 
> Clinical Laboratory license. I have been a Histotech and have worked in IHC 
> for 20+ years and was required to obtain a NY State Clinical Lab License in 
> 2007. I don't have and associates or bachelor degree and was not required to 
> prior to 2007. I was told on a job interview that if I don't have either of 
> these degrees that I cannot gross any specimens or run IHC. I've never heard 
> this before. Has anyone else ever heard of this??? Thanks - Gail
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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RE: [Histonet] histology in higher education

[Joelle Weaver]

Many people do this, and have donated hundreds of hours of their own time. But 
definately not enough. Good to encourage people to get involved.


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
> Date: Wed, 22 Apr 2015 14:07:18 +
> From: koelli...@comcast.net
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] histology in higher education
> 
> The following has to do with histology and STEM (science, technology, 
> engineering, math) so if not interested, please ignore.  But I believe it can 
> have real meaning to the profession of histology at the NSH, state society 
> and local levels. 
>   
> I am elected to the Board of WSSEF (Washington State Science and Engineering 
> Fair) where I am in educational outreach and also the assistant to the head 
> judge.  We recently had our Washington State Fair with 650 kids, grades 1-12 
> from all over the state.  And while there was a lot of engineering and robots 
> and computers there were a few projects having to do with medicine, 
> biotechnology, immunology and pathology with some familiar histology or 
> immunohistochemistry pictures included.  At the end of the fair, we awarded 
> almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 
> students.  Not only that, our top winners get an all-expenses paid trip to 
> present at the ISEF (Intel International Fair) with 1,700 students competing 
> from all 50 states and 70 countries.  Wherever you are in the US, you have a 
> state fair. 
>   
> I would advocate for some of you so interested at the national, state or 
> local levels to promote histology, by getting involved as mentors for middle 
> and high school students to science fairs; especially those that could lead 
> to histopathology or other related projects that could lead into Intel 
> affiliated fairs resulting in great benefit to the student and a spread of 
> the word of histology into both the STEM world and general population. 
>   
> I've mentored for 15 years.  It can be done.  Molecular histopathology, 
> personalized diagnostics and therapeutics, advances in immunohistochemistry, 
> current controversies about breast biopsy diagnosis, or other disease with 
> newer classifications, PCR and RTPCR in histology, modern-targeted 
> therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image 
> analysis software for you computer geeks and on and on; the list is nearly 
> limitless.  Especially if you are close to or can contact biotech companies 
> or educational institutions to find co-mentors for grades 7-12 there are 
> histology-related science project possibilities in terms of data collection 
> and the scientific method and project presentation are nearly unlimited now. 
>   
> Be a mentor for or engage a grade 7-12 student, with the help of another 
> mentor or organization, to think about (histology-related) projects for 
> science fairs leading to a state fair and Intel ISEF.  Can't think of any 
> better way to "promote histology" so would hope those at NSH would take note 
> of this.  And since the ISEF fair receives projects and groups from 70 
> countries, I hope any outside the US would also think about the same thing. 
>   
> Ray Koelling 
> HT, HTL, QIHC, STEM educational outreach advocate 
> Lake Forest Park, WA 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
___
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RE: [Histonet] NY State regulations

[Joelle Weaver]

Probably CLIA related to high complexity testing. IHC is not considered under 
CLIA ( from 1988), though many people feel otherwise. Grossing is. I think that 
it is under sub part G or H if I remember correctly. 


Joelle Weaver MAOM, HTL (ASCP) QIHC


  

 
> From: gmarce...@nj-urology.com
> To: histonet@lists.utsouthwestern.edu
> Date: Wed, 22 Apr 2015 10:55:19 -0400
> Subject: [Histonet] NY State regulations
> 
> Hi - I was wondering if anyone knows the regulations regarding the NY State 
> Clinical Laboratory license. I have been a Histotech and have worked in IHC 
> for 20+ years and was required to obtain a NY State Clinical Lab License in 
> 2007. I don't have and associates or bachelor degree and was not required to 
> prior to 2007. I was told on a job interview that if I don't have either of 
> these degrees that I cannot gross any specimens or run IHC. I've never heard 
> this before. Has anyone else ever heard of this??? Thanks - Gail
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
___
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RE: [Histonet] RE: Nuclear "Artifact"

[Arbaugh, Roberta]

We have had the same problem. We come to the conclusion that it was water 
droplets. We had the problem when our humidity was high in the lab, or our 
weekend run. The changes we made where :
1. We no longer process over the weekend . We cannot count on our heating and 
cooling in our building.
2. We place a towel and a thick piece of cardboard on top of the processor lid.
3.We do not use the really fine mesh cassettes. We will use formalin soaked 
sponges or perm papers.
4. We do not over pack cassette basket.
We had our processor check every time we saw the artifact and they could never 
find a problem with the processor.
Hope this help, Roberta

-Original Message-
From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu]
Sent: Wednesday, April 22, 2015 11:16 AM
To: Sue; Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Nuclear "Artifact"

"One pathologist said it looks like the tissue has been cooked."  Which could 
also be drying artifact after bx, before formalin.

"The only issue is we can have two biopsies right next to one another in the 
basket one looks good and one looks bad. My director also thinks it is the 
processors."

Same processor but two results? Solving intermittent problems takes time to 
check variables - time almost no one wants to spend to check out every 
possibility. But if one variable is the same for both, and the result is 
different, then most likely it is a different variable causing the problem.

I think the other idea suggested of checking the handling of the tissue at the 
source of the biopsy is more likely to shed some light on this issue.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, April 21, 2015 4:55 PM
To: Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Nuclear "Artifact"

OMG we are experiencing the same issue. At first it was just GI and now we are 
seeing it on prostate. One pathologist said it looks like the tissue has been 
cooked. The only issue is we can have two biopsies right next to one another in 
the basket one looks good and one looks bad. My director also thinks it is the 
processors. I had Thermo out and they could find nothing. We changed out all 
the reagents and the biopsies were fine than two days later we had some bad 
ones. I know in July Fisher had a formalin recall associated to the mixture of 
buffer, water and formalin. We thought that might be it but it is now almost a 
year later and all the bad formalin should be gone. The histotechs say the 
tissue is crunchy and they are right. I am running a test tonight of a small 
needle biopsy that I made from a colon. I placed it is straight formaldehyde 
overnight and am processing it on our biopsy cycle tonight. My director also 
wanted us to only put three levels on our Thermo, but he wanted the middle 
level to have empty baskets. I stopped that today because I think the other 
issue is that the poor biopsies may be on the top level and as the reagents are 
used the level changes, and also due to displacement with the middle level 
being empty the reagent levels may not reach the top. We just do not have the 
manpower to inspect every reagent every day, we have 6 processor and it would 
take a tech all day. We actually take a digital picture when they come out of 
the processor. I want to check my problems cases tomorrow. We do not use 
sponges but the only other like was the PA who was wrapping the blue paper very 
tight around the tissue. I really do not think this is the issue though.. Any 
other insight would be greatly appreciated.

Susan T. Paturzo
TJUH
___
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immediately contact the sender if you have received this message in error. 
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RE: [Histonet] Nuclear "Artifact"

[Kienitz, Kari]

I have experience the over-cooked, dried out look a few times myself through 
the years and it can be baffeling especially when pathologists tell you things 
look "dried out".  The first thing we think of it too much exposure to 
heat/chemical when its almost always not enough exposure to xylene at the 
deparaffinization stage or the tissue is underprocessed. Both of these 
scenarios can make tissue looked dried out. Make sure your protocols are 
adequate for processing thickness. Then there are the varying paraffin 
compounds.  the higher the polymer content the more time you need in 
xyleneI know it sounds crazy but increase your time in xylene prior to 
staining and you will see alot of your random staining issues disappear.


Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
 NE 99th Ave
Portland, OR  97220
503.935.8311
kkien...@orclinic.com




CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive 
and confidential use of the intended recipient. If you are not the intended 
recipient, please do not read, distribute or take action in reliance upon this 
missive. If you have received this in error, please notify the sender 
immediately by reply e-mail and delete this message and its attachments from 
your computer system. Thank you

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patrick Laurie 
[foreig...@gmail.com]
Sent: Wednesday, April 22, 2015 9:10 AM
To: Michael Ann Jones
Cc: histonet@lists.utsouthwestern.edu; Lisa Roy
Subject: Re: [Histonet] Nuclear "Artifact"

At a previous job, we found (this was specific to prostate biopsies) that
one of our clients was taking the prostate biopsies out, lining them up on
a dry paper towel, doing the whole procedure, then putting them into the
formalin jars.  It can be some time between the start and finish of a
procedure, so the first couple were looking very dried out (paper towel
absorbed most of the moisture) with very pale hematoxylin staining.  The
pathologist found them almost uninterpretable. When this happened, the
first natural area we examined was processing, which in this case turned
out not to be the culprit.  Collection procedures are not usually done by
lab staff, a clinician or staff may have a great idea to make things easier
but not know the downstream effects.

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869

On Wed, Apr 22, 2015 at 10:02 AM, Michael Ann Jones 
wrote:

> Happy Lab Week!!
> We worked on our H&E for almost two years. We were using Leica H&E
> products and after 2 years of struggling, adjusting and analyzing
> everything in our lab - we switched to the Richard-Allen Scientific
> products. We were seeing variability between days of staining, tissues
> right next to each other, nuclear paleness, eosin uniformity instead of
> differentiated, etc. Switching reagents helped us tremendously - we have
> more consistent higher quality stains on a daily basis and within tissues.
>
> I you¹re struggling and have analyzed your processors, etc. to death -
> maybe try different reagents? (we even measured the tap water that we use
> on our stainer daily, the pH of reagents every other hour etc. and between
> 5 experienced histotechs, we couldn¹t figure it out)
> Good luck! :)
>
> Michael Ann
> Michael Ann Jones, HT (ASCP)
> Histology Manager
> Metropath
> 7444 W. Alaska Dr. #250
> Lakewood, CO 80226
> 303.634.2511
> mjo...@metropath.com
>
>
>
>
>
>
>
> On 4/21/15, 5:55 PM, "Sue"  wrote:
>
> >OMG we are experiencing the same issue. At first it was just GI and now
> >we are seeing it on prostate. One pathologist said it looks like the
> >tissue has been cooked. The only issue is we can have two biopsies right
> >next to one another in the basket one looks good and one looks bad. My
> >director also thinks it is the processors. I had Thermo out and they
> >could find nothing. We changed out all the reagents and the biopsies were
> >fine than two days later we had some bad ones. I know in July Fisher had
> >a formalin recall associated to the mixture of buffer, water and
> >formalin. We thought that might be it but it is now almost a year later
> >and all the bad formalin should be gone. The histotechs say the tissue is
> >crunchy and they are right. I am running a test tonight of a small needle
> >biopsy that I made from a colon. I placed it is straight formaldehyde
> >overnight and am processing it on our biopsy cycle tonight. My director
> >also wanted us to only put three levels on our Thermo, but he wanted the
> >middle level to have empty baskets. I stopped that today because I think
> >the other issue is that the poor biopsies may be on the top level and as
> >the reagents are used the level changes, and also due to displacement
> >with the middle 

RE: [Histonet] histology in higher education

[Piche, Jessica]

Thank you for sharing this information Andi. I'd like to do something like this 
and I'm going to send this on to my daughters science teachers at school. I 
think it's a great idea. It always amazed me all the different jobs in 
hospitals alone that are available for kids and adults alike and no one knows 
they exist. Especially histology. Looking forward to passing this on!
Thanks again,
Jessica Piche, HT(ASCP)
Waterbury Hospital
CT

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Wednesday, April 22, 2015 12:01 PM
To: ""
Subject: RE: [Histonet] histology in higher education

Bonnie, and anybody who wants to do this:
www.prescientist.org



From: Whitaker, Bonnie [bonnie.whita...@osumc.edu]
Sent: Wednesday, April 22, 2015 8:45 AM
To: Grantham, Andrea L - (algranth); ""
Subject: RE: [Histonet] histology in higher education

Andi,

Would you be willing to share the information on how to volunteer with this 
program?

Thanks,
Bonnie

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Wednesday, April 22, 2015 11:40 AM
To: ""
Subject: RE: [Histonet] histology in higher education

For a few years I've been involved in a program called "letters to a 
pre-scientist". The idea is to reach middle schoolers as they are being 
introduced to the sciences. They have pretty high goals at this time, they want 
to be doctors and astronauts and engineers but they are just starting to learn 
about these things.
You become a pen pal/mentor of sorts and write letters to a child and they will 
write back to you. Last year I was writing to a boy in the Chicago area and 
this year it was a girl in LA. I always write about what I do and how important 
it is and include pictures of things like brain cells, muscle, fungus, bacteria 
and pictures of my lab. I always pick up a copy of the NSH coloring book and 
send it to them and tell them what they need to study to be a histotech and 
other than a hospital, where they can find a job. Of course we also tell them 
about other things like our families, pets, vacations, etc. at the same time.

It's just a small thing but it plants a seed.

Andi G.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
wsim...@athensgastro.com [wsim...@athensgastro.com]
Sent: Wednesday, April 22, 2015 7:31 AM
To: koelli...@comcast.net; ""
Subject: Re: [Histonet] histology in higher education

Good morning Ray and thank you for promoting the field of Histotechnology.

While President of the NSH, Vinnie DellaSperanza started a career day function 
at the annual NSH symposium.  It has been very successful and the individuals 
that contribute to this volunteer effort are usually the same individuals that 
participate at the state and regional level.
Thank you for the idea of state fairs and other avenues for the target age of 
middle & high school students.
I did this myself when my children were in girl scouts , "Odyssey of the Mind" 
and advance placement opportunities.


Wanda K. Simons, HT (ASCP)
GSH President
www.histosearch.com/gsh/


>  ---Original Message---
>  From: koelli...@comcast.net
>  To: histonet@lists.utsouthwestern.edu
>  Subject: [Histonet] histology in higher education
>  Sent: Apr 22 '15 10:14
>
>  The following has to do with histology and STEM (science, technology, 
> engineering, math) so if not interested, please ignore.  But I believe it can 
> have real meaning to the profession of histology at the NSH, state society 
> and local levels.
>
>  I am elected to the Board of WSSEF (Washington State Science and Engineering 
> Fair) where I am in educational outreach and also the assistant to the head 
> judge.  We recently had our Washington State Fair with 650 kids, grades 1-12 
> from all over the state.  And while there was a lot of engineering and robots 
> and computers there were a few projects having to do with medicine, 
> biotechnology, immunology and pathology with some familiar histology or 
> immunohistochemistry pictures included.  At the end of the fair, we awarded 
> almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 
> students.  Not only that, our top winners get an all-expenses paid trip to 
> present at the ISEF (Intel International Fair) with 1,700 students competing 
> from all 50 states and 70 countries.  Wherever you are in the US, you have a 
> state fair.
>
>  I would advocate for some of you so interested at the national, state or 
> local levels to promote histology, by getting involved as mentors for middle 
> and high school students to science fairs; especially those that could lead 
> to histopathology or other related projects that could lead

Re: [Histonet] Nuclear "Artifact"

[Patrick Laurie]

At a previous job, we found (this was specific to prostate biopsies) that
one of our clients was taking the prostate biopsies out, lining them up on
a dry paper towel, doing the whole procedure, then putting them into the
formalin jars.  It can be some time between the start and finish of a
procedure, so the first couple were looking very dried out (paper towel
absorbed most of the moisture) with very pale hematoxylin staining.  The
pathologist found them almost uninterpretable. When this happened, the
first natural area we examined was processing, which in this case turned
out not to be the culprit.  Collection procedures are not usually done by
lab staff, a clinician or staff may have a great idea to make things easier
but not know the downstream effects.

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869

On Wed, Apr 22, 2015 at 10:02 AM, Michael Ann Jones 
wrote:

> Happy Lab Week!!
> We worked on our H&E for almost two years. We were using Leica H&E
> products and after 2 years of struggling, adjusting and analyzing
> everything in our lab - we switched to the Richard-Allen Scientific
> products. We were seeing variability between days of staining, tissues
> right next to each other, nuclear paleness, eosin uniformity instead of
> differentiated, etc. Switching reagents helped us tremendously - we have
> more consistent higher quality stains on a daily basis and within tissues.
>
> I you¹re struggling and have analyzed your processors, etc. to death -
> maybe try different reagents? (we even measured the tap water that we use
> on our stainer daily, the pH of reagents every other hour etc. and between
> 5 experienced histotechs, we couldn¹t figure it out)
> Good luck! :)
>
> Michael Ann
> Michael Ann Jones, HT (ASCP)
> Histology Manager
> Metropath
> 7444 W. Alaska Dr. #250
> Lakewood, CO 80226
> 303.634.2511
> mjo...@metropath.com
>
>
>
>
>
>
>
> On 4/21/15, 5:55 PM, "Sue"  wrote:
>
> >OMG we are experiencing the same issue. At first it was just GI and now
> >we are seeing it on prostate. One pathologist said it looks like the
> >tissue has been cooked. The only issue is we can have two biopsies right
> >next to one another in the basket one looks good and one looks bad. My
> >director also thinks it is the processors. I had Thermo out and they
> >could find nothing. We changed out all the reagents and the biopsies were
> >fine than two days later we had some bad ones. I know in July Fisher had
> >a formalin recall associated to the mixture of buffer, water and
> >formalin. We thought that might be it but it is now almost a year later
> >and all the bad formalin should be gone. The histotechs say the tissue is
> >crunchy and they are right. I am running a test tonight of a small needle
> >biopsy that I made from a colon. I placed it is straight formaldehyde
> >overnight and am processing it on our biopsy cycle tonight. My director
> >also wanted us to only put three levels on our Thermo, but he wanted the
> >middle level to have empty baskets. I stopped that today because I think
> >the other issue is that the poor biopsies may be on the top level and as
> >the reagents are used the level changes, and also due to displacement
> >with the middle level being empty the reagent levels may not reach the
> >top. We just do not have the manpower to inspect every reagent every day,
> >we have 6 processor and it would take a tech all day. We actually take a
> >digital picture when they come out of the processor. I want to check my
> >problems cases tomorrow. We do not use sponges but the only other like
> >was the PA who was wrapping the blue paper very tight around the tissue.
> >I really do not think this is the issue though.. Any other insight would
> >be greatly appreciated.
> >
> >Susan T. Paturzo
> >TJUH
> >___
> >Histonet mailing list
> >Histonet@lists.utsouthwestern.edu
> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] histology in higher education

[Grantham, Andrea L - (algranth)]

Bonnie, and anybody who wants to do this:
www.prescientist.org



From: Whitaker, Bonnie [bonnie.whita...@osumc.edu]
Sent: Wednesday, April 22, 2015 8:45 AM
To: Grantham, Andrea L - (algranth); ""
Subject: RE: [Histonet] histology in higher education

Andi,

Would you be willing to share the information on how to volunteer with this 
program?

Thanks,
Bonnie

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Wednesday, April 22, 2015 11:40 AM
To: ""
Subject: RE: [Histonet] histology in higher education

For a few years I've been involved in a program called "letters to a 
pre-scientist". The idea is to reach middle schoolers as they are being 
introduced to the sciences. They have pretty high goals at this time, they want 
to be doctors and astronauts and engineers but they are just starting to learn 
about these things.
You become a pen pal/mentor of sorts and write letters to a child and they will 
write back to you. Last year I was writing to a boy in the Chicago area and 
this year it was a girl in LA. I always write about what I do and how important 
it is and include pictures of things like brain cells, muscle, fungus, bacteria 
and pictures of my lab. I always pick up a copy of the NSH coloring book and 
send it to them and tell them what they need to study to be a histotech and 
other than a hospital, where they can find a job. Of course we also tell them 
about other things like our families, pets, vacations, etc. at the same time.

It's just a small thing but it plants a seed.

Andi G.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
wsim...@athensgastro.com [wsim...@athensgastro.com]
Sent: Wednesday, April 22, 2015 7:31 AM
To: koelli...@comcast.net; ""
Subject: Re: [Histonet] histology in higher education

Good morning Ray and thank you for promoting the field of Histotechnology.

While President of the NSH, Vinnie DellaSperanza started a career day function 
at the annual NSH symposium.  It has been very successful and the individuals 
that contribute to this volunteer effort are usually the same individuals that 
participate at the state and regional level.
Thank you for the idea of state fairs and other avenues for the target age of 
middle & high school students.
I did this myself when my children were in girl scouts , "Odyssey of the Mind" 
and advance placement opportunities.


Wanda K. Simons, HT (ASCP)
GSH President
www.histosearch.com/gsh/


>  ---Original Message---
>  From: koelli...@comcast.net
>  To: histonet@lists.utsouthwestern.edu
>  Subject: [Histonet] histology in higher education
>  Sent: Apr 22 '15 10:14
>
>  The following has to do with histology and STEM (science, technology, 
> engineering, math) so if not interested, please ignore.  But I believe it can 
> have real meaning to the profession of histology at the NSH, state society 
> and local levels.
>
>  I am elected to the Board of WSSEF (Washington State Science and Engineering 
> Fair) where I am in educational outreach and also the assistant to the head 
> judge.  We recently had our Washington State Fair with 650 kids, grades 1-12 
> from all over the state.  And while there was a lot of engineering and robots 
> and computers there were a few projects having to do with medicine, 
> biotechnology, immunology and pathology with some familiar histology or 
> immunohistochemistry pictures included.  At the end of the fair, we awarded 
> almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 
> students.  Not only that, our top winners get an all-expenses paid trip to 
> present at the ISEF (Intel International Fair) with 1,700 students competing 
> from all 50 states and 70 countries.  Wherever you are in the US, you have a 
> state fair.
>
>  I would advocate for some of you so interested at the national, state or 
> local levels to promote histology, by getting involved as mentors for middle 
> and high school students to science fairs; especially those that could lead 
> to histopathology or other related projects that could lead into Intel 
> affiliated fairs resulting in great benefit to the student and a spread of 
> the word of histology into both the STEM world and general population.
>
>  I've mentored for 15 years.  It can be done.  Molecular histopathology, 
> personalized diagnostics and therapeutics, advances in immunohistochemistry, 
> current controversies about breast biopsy diagnosis, or other disease with 
> newer classifications, PCR and RTPCR in histology, modern-targeted 
> therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image 
> analysis software for you computer geeks and on and on; the list is nearly 
> limitless.  Especially if you are close to or can contact biotech companies 
> or educa

RE: [Histonet] histology in higher education

[Whitaker, Bonnie]

Andi,

Would you be willing to share the information on how to volunteer with this 
program?

Thanks,
Bonnie

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham, 
Andrea L - (algranth)
Sent: Wednesday, April 22, 2015 11:40 AM
To: ""
Subject: RE: [Histonet] histology in higher education

For a few years I've been involved in a program called "letters to a 
pre-scientist". The idea is to reach middle schoolers as they are being 
introduced to the sciences. They have pretty high goals at this time, they want 
to be doctors and astronauts and engineers but they are just starting to learn 
about these things.
You become a pen pal/mentor of sorts and write letters to a child and they will 
write back to you. Last year I was writing to a boy in the Chicago area and 
this year it was a girl in LA. I always write about what I do and how important 
it is and include pictures of things like brain cells, muscle, fungus, bacteria 
and pictures of my lab. I always pick up a copy of the NSH coloring book and 
send it to them and tell them what they need to study to be a histotech and 
other than a hospital, where they can find a job. Of course we also tell them 
about other things like our families, pets, vacations, etc. at the same time. 

It's just a small thing but it plants a seed.

Andi G.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
wsim...@athensgastro.com [wsim...@athensgastro.com]
Sent: Wednesday, April 22, 2015 7:31 AM
To: koelli...@comcast.net; ""
Subject: Re: [Histonet] histology in higher education

Good morning Ray and thank you for promoting the field of Histotechnology.

While President of the NSH, Vinnie DellaSperanza started a career day function 
at the annual NSH symposium.  It has been very successful and the individuals 
that contribute to this volunteer effort are usually the same individuals that 
participate at the state and regional level.
Thank you for the idea of state fairs and other avenues for the target age of 
middle & high school students.
I did this myself when my children were in girl scouts , "Odyssey of the Mind" 
and advance placement opportunities.


Wanda K. Simons, HT (ASCP)
GSH President
www.histosearch.com/gsh/


>  ---Original Message---
>  From: koelli...@comcast.net
>  To: histonet@lists.utsouthwestern.edu
>  Subject: [Histonet] histology in higher education
>  Sent: Apr 22 '15 10:14
>
>  The following has to do with histology and STEM (science, technology, 
> engineering, math) so if not interested, please ignore.  But I believe it can 
> have real meaning to the profession of histology at the NSH, state society 
> and local levels.
>
>  I am elected to the Board of WSSEF (Washington State Science and Engineering 
> Fair) where I am in educational outreach and also the assistant to the head 
> judge.  We recently had our Washington State Fair with 650 kids, grades 1-12 
> from all over the state.  And while there was a lot of engineering and robots 
> and computers there were a few projects having to do with medicine, 
> biotechnology, immunology and pathology with some familiar histology or 
> immunohistochemistry pictures included.  At the end of the fair, we awarded 
> almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 
> students.  Not only that, our top winners get an all-expenses paid trip to 
> present at the ISEF (Intel International Fair) with 1,700 students competing 
> from all 50 states and 70 countries.  Wherever you are in the US, you have a 
> state fair.
>
>  I would advocate for some of you so interested at the national, state or 
> local levels to promote histology, by getting involved as mentors for middle 
> and high school students to science fairs; especially those that could lead 
> to histopathology or other related projects that could lead into Intel 
> affiliated fairs resulting in great benefit to the student and a spread of 
> the word of histology into both the STEM world and general population.
>
>  I've mentored for 15 years.  It can be done.  Molecular histopathology, 
> personalized diagnostics and therapeutics, advances in immunohistochemistry, 
> current controversies about breast biopsy diagnosis, or other disease with 
> newer classifications, PCR and RTPCR in histology, modern-targeted 
> therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image 
> analysis software for you computer geeks and on and on; the list is nearly 
> limitless.  Especially if you are close to or can contact biotech companies 
> or educational institutions to find co-mentors for grades 7-12 there are 
> histology-related science project possibilities in terms of data collection 
> and the scientific method and project presentation are nearly unlimited now.
>
>  Be a mentor for or engage a grade 7-12 student, with the help 

RE: [Histonet] histology in higher education

[Grantham, Andrea L - (algranth)]

For a few years I've been involved in a program called "letters to a 
pre-scientist". The idea is to reach middle schoolers as they are being 
introduced to the sciences. They have pretty high goals at this time, they want 
to be doctors and astronauts and engineers but they are just starting to learn 
about these things.
You become a pen pal/mentor of sorts and write letters to a child and they will 
write back to you. Last year I was writing to a boy in the Chicago area and 
this year it was a girl in LA. I always write about what I do and how important 
it is and include pictures of things like brain cells, muscle, fungus, bacteria 
and pictures of my lab. I always pick up a copy of the NSH coloring book and 
send it to them and tell them what they need to study to be a histotech and 
other than a hospital, where they can find a job. Of course we also tell them 
about other things like our families, pets, vacations, etc. at the same time. 

It's just a small thing but it plants a seed.

Andi G.

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of 
wsim...@athensgastro.com [wsim...@athensgastro.com]
Sent: Wednesday, April 22, 2015 7:31 AM
To: koelli...@comcast.net; ""
Subject: Re: [Histonet] histology in higher education

Good morning Ray and thank you for promoting the field of Histotechnology.

While President of the NSH, Vinnie DellaSperanza started a career day function 
at the annual NSH
symposium.  It has been very successful and the individuals that contribute to 
this volunteer effort are usually
the same individuals that participate at the state and regional level.
Thank you for the idea of state fairs and other avenues for the target age of 
middle & high school students.
I did this myself when my children were in girl scouts , "Odyssey of the Mind" 
and advance placement opportunities.


Wanda K. Simons, HT (ASCP)
GSH President
www.histosearch.com/gsh/


>  ---Original Message---
>  From: koelli...@comcast.net
>  To: histonet@lists.utsouthwestern.edu
>  Subject: [Histonet] histology in higher education
>  Sent: Apr 22 '15 10:14
>
>  The following has to do with histology and STEM (science, technology, 
> engineering, math) so if not interested, please ignore.  But I believe it can 
> have real meaning to the profession of histology at the NSH, state society 
> and local levels.
>
>  I am elected to the Board of WSSEF (Washington State Science and Engineering 
> Fair) where I am in educational outreach and also the assistant to the head 
> judge.  We recently had our Washington State Fair with 650 kids, grades 1-12 
> from all over the state.  And while there was a lot of engineering and robots 
> and computers there were a few projects having to do with medicine, 
> biotechnology, immunology and pathology with some familiar histology or 
> immunohistochemistry pictures included.  At the end of the fair, we awarded 
> almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 
> students.  Not only that, our top winners get an all-expenses paid trip to 
> present at the ISEF (Intel International Fair) with 1,700 students competing 
> from all 50 states and 70 countries.  Wherever you are in the US, you have a 
> state fair.
>
>  I would advocate for some of you so interested at the national, state or 
> local levels to promote histology, by getting involved as mentors for middle 
> and high school students to science fairs; especially those that could lead 
> to histopathology or other related projects that could lead into Intel 
> affiliated fairs resulting in great benefit to the student and a spread of 
> the word of histology into both the STEM world and general population.
>
>  I've mentored for 15 years.  It can be done.  Molecular histopathology, 
> personalized diagnostics and therapeutics, advances in immunohistochemistry, 
> current controversies about breast biopsy diagnosis, or other disease with 
> newer classifications, PCR and RTPCR in histology, modern-targeted 
> therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image 
> analysis software for you computer geeks and on and on; the list is nearly 
> limitless.  Especially if you are close to or can contact biotech companies 
> or educational institutions to find co-mentors for grades 7-12 there are 
> histology-related science project possibilities in terms of data collection 
> and the scientific method and project presentation are nearly unlimited now.
>
>  Be a mentor for or engage a grade 7-12 student, with the help of another 
> mentor or organization, to think about (histology-related) projects for 
> science fairs leading to a state fair and Intel ISEF.  Can't think of any 
> better way to "promote histology" so would hope those at NSH would take note 
> of this.  And since the ISEF fair receives projects and groups from 70 
> countries, I hope any outside the US would also think about the sam

[Histonet] RE: Nuclear "Artifact"

[Morken, Timothy]

"One pathologist said it looks like the tissue has been cooked."  Which could 
also be drying artifact after bx, before formalin.

"The only issue is we can have two biopsies right next to one another in the 
basket one looks good and one looks bad. My director also thinks it is the 
processors."

Same processor but two results? Solving intermittent problems takes time to 
check variables - time almost no one wants to spend to check out every 
possibility. But if one variable is the same for both, and the result is 
different, then most likely it is a different variable causing the problem.

I think the other idea suggested of checking the handling of the tissue at the 
source of the biopsy is more likely to shed some light on this issue. 


Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center





-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue
Sent: Tuesday, April 21, 2015 4:55 PM
To: Lisa Roy
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Nuclear "Artifact"

OMG we are experiencing the same issue. At first it was just GI and now we are 
seeing it on prostate. One pathologist said it looks like the tissue has been 
cooked. The only issue is we can have two biopsies right next to one another in 
the basket one looks good and one looks bad. My director also thinks it is the 
processors. I had Thermo out and they could find nothing. We changed out all 
the reagents and the biopsies were fine than two days later we had some bad 
ones. I know in July Fisher had a formalin recall associated to the mixture of 
buffer, water and formalin. We thought that might be it but it is now almost a 
year later and all the bad formalin should be gone. The histotechs say the 
tissue is crunchy and they are right. I am running a test tonight of a small 
needle biopsy that I made from a colon. I placed it is straight formaldehyde 
overnight and am processing it on our biopsy cycle tonight. My director also 
wanted us to only put three levels on our Thermo, but he wanted the middle 
level to have empty baskets. I stopped that today because I think the other 
issue is that the poor biopsies may be on the top level and as the reagents are 
used the level changes, and also due to displacement with the middle level 
being empty the reagent levels may not reach the top. We just do not have the 
manpower to inspect every reagent every day, we have 6 processor and it would 
take a tech all day. We actually take a digital picture when they come out of 
the processor. I want to check my problems cases tomorrow. We do not use 
sponges but the only other like was the PA who was wrapping the blue paper very 
tight around the tissue. I really do not think this is the issue though.. Any 
other insight would be greatly appreciated. 

Susan T. Paturzo 
TJUH 
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RE: [Histonet] IHC/SS QA/QC Sheets:

[Sebree Linda A]

Hi Craig,

As technologists, we of course QC all slides before they get to the 
pathologist(s) but that is not documented unless we put a repeat order in the 
case.  Then we add a note saying why a test needs repeating, i.e. positive 
control did not stain, patient tissue loss, etc.  But this is all done 
electronically, so no paper trail.  For general QC, we have gone to having a 
statement automatically populate to any case reports that include IHC/ISH 
staining but I'm assuming this could also work for H&Es, Special Stains, etc.; 
I'm only familiar with the IHC/ISH portion of QCing.  Our statement says 
something along the lines of the controls staining appropriately and as 
expected.  The pathologist that signs out the case is attesting to that 
statement.  This statement has eliminated HUGE amounts of paper and filing.

Hope this helps,

Linda


Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory, Rm A4/204-3224 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb
Sent: Tuesday, April 21, 2015 3:16 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC/SS QA/QC Sheets:

Can someone help guide me on the right direction regarding how to organize 
daily QC sheets. Currently we have one per case (this is time consuming and I'm 
on overload of papers). Does anyone have a good solution and are you willing to 
share?

Thank you for your help,

Craig

Sent from my iPhone
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[Histonet] NY State regulations

[Gail Marcella]

Hi - I was wondering if anyone knows the regulations regarding the NY State 
Clinical Laboratory license. I have been a Histotech and have worked in IHC for 
20+ years and was required to obtain a NY State Clinical Lab License in 2007. I 
don't have and associates or bachelor degree and was not required to prior to 
2007. I was told on a job interview that if I don't have either of these 
degrees that I cannot gross any specimens or run IHC. I've never heard this 
before. Has anyone else ever heard of this??? Thanks - Gail
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[Histonet] Need Daniel Terry exTBS new company info

[Ross Stapf]

I was told that Daniel Terry left TBS to work for a private biomed company.  I 
am looking for contact info on this company.  He was their main person for the 
ATP processor.  There are too many other Daniel Terry's in that area for google 
to help.  Hopefully someone here uses his new company.

Ross M Stapf HT(ASCP)

Dallas Associated Dermatologists, P.A.
12700 Park Central Drive
Suite B-150
Dallas, TX 75251

Phone # 214-987-3376 x3396
Fax # (214) 420-1441

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Re: [Histonet] histology in higher education

[wsimons]

Good morning Ray and thank you for promoting the field of Histotechnology.

While President of the NSH, Vinnie DellaSperanza started a career day function 
at the annual NSH
symposium.  It has been very successful and the individuals that contribute to 
this volunteer effort are usually
the same individuals that participate at the state and regional level.   
Thank you for the idea of state fairs and other avenues for the target age of 
middle & high school students.
I did this myself when my children were in girl scouts , "Odyssey of the Mind" 
and advance placement opportunities.


Wanda K. Simons, HT (ASCP)
GSH President
www.histosearch.com/gsh/


>  ---Original Message---
>  From: koelli...@comcast.net
>  To: histonet@lists.utsouthwestern.edu
>  Subject: [Histonet] histology in higher education
>  Sent: Apr 22 '15 10:14
>  
>  The following has to do with histology and STEM (science, technology, 
> engineering, math) so if not interested, please ignore.  But I believe it can 
> have real meaning to the profession of histology at the NSH, state society 
> and local levels.
>   
>  I am elected to the Board of WSSEF (Washington State Science and Engineering 
> Fair) where I am in educational outreach and also the assistant to the head 
> judge.  We recently had our Washington State Fair with 650 kids, grades 1-12 
> from all over the state.  And while there was a lot of engineering and robots 
> and computers there were a few projects having to do with medicine, 
> biotechnology, immunology and pathology with some familiar histology or 
> immunohistochemistry pictures included.  At the end of the fair, we awarded 
> almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 
> students.  Not only that, our top winners get an all-expenses paid trip to 
> present at the ISEF (Intel International Fair) with 1,700 students competing 
> from all 50 states and 70 countries.  Wherever you are in the US, you have a 
> state fair.
>   
>  I would advocate for some of you so interested at the national, state or 
> local levels to promote histology, by getting involved as mentors for middle 
> and high school students to science fairs; especially those that could lead 
> to histopathology or other related projects that could lead into Intel 
> affiliated fairs resulting in great benefit to the student and a spread of 
> the word of histology into both the STEM world and general population.
>   
>  I've mentored for 15 years.  It can be done.  Molecular histopathology, 
> personalized diagnostics and therapeutics, advances in immunohistochemistry, 
> current controversies about breast biopsy diagnosis, or other disease with 
> newer classifications, PCR and RTPCR in histology, modern-targeted 
> therapeutics like in melanoma or colo-rectal carcinoma, FISH, digital image 
> analysis software for you computer geeks and on and on; the list is nearly 
> limitless.  Especially if you are close to or can contact biotech companies 
> or educational institutions to find co-mentors for grades 7-12 there are 
> histology-related science project possibilities in terms of data collection 
> and the scientific method and project presentation are nearly unlimited now.
>   
>  Be a mentor for or engage a grade 7-12 student, with the help of another 
> mentor or organization, to think about (histology-related) projects for 
> science fairs leading to a state fair and Intel ISEF.  Can't think of any 
> better way to "promote histology" so would hope those at NSH would take note 
> of this.  And since the ISEF fair receives projects and groups from 70 
> countries, I hope any outside the US would also think about the same thing.
>   
>  Ray Koelling
>  HT, HTL, QIHC, STEM educational outreach advocate
>  Lake Forest Park, WA
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>  http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  

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[Histonet] histology in higher education

[koellingr]

The following has to do with histology and STEM (science, technology, 
engineering, math) so if not interested, please ignore.  But I believe it can 
have real meaning to the profession of histology at the NSH, state society and 
local levels. 
  
I am elected to the Board of WSSEF (Washington State Science and Engineering 
Fair) where I am in educational outreach and also the assistant to the head 
judge.  We recently had our Washington State Fair with 650 kids, grades 1-12 
from all over the state.  And while there was a lot of engineering and robots 
and computers there were a few projects having to do with medicine, 
biotechnology, immunology and pathology with some familiar histology or 
immunohistochemistry pictures included.  At the end of the fair, we awarded 
almost 1.8 MILLION dollars of scholarships and awards to grades 7-12 students.  
Not only that, our top winners get an all-expenses paid trip to present at the 
ISEF (Intel International Fair) with 1,700 students competing from all 50 
states and 70 countries.  Wherever you are in the US, you have a state fair. 
  
I would advocate for some of you so interested at the national, state or local 
levels to promote histology, by getting involved as mentors for middle and high 
school students to science fairs; especially those that could lead to 
histopathology or other related projects that could lead into Intel affiliated 
fairs resulting in great benefit to the student and a spread of the word of 
histology into both the STEM world and general population. 
  
I've mentored for 15 years.  It can be done.  Molecular histopathology, 
personalized diagnostics and therapeutics, advances in immunohistochemistry, 
current controversies about breast biopsy diagnosis, or other disease with 
newer classifications, PCR and RTPCR in histology, modern-targeted therapeutics 
like in melanoma or colo-rectal carcinoma, FISH, digital image analysis 
software for you computer geeks and on and on; the list is nearly limitless.  
Especially if you are close to or can contact biotech companies or educational 
institutions to find co-mentors for grades 7-12 there are histology-related 
science project possibilities in terms of data collection and the scientific 
method and project presentation are nearly unlimited now. 
  
Be a mentor for or engage a grade 7-12 student, with the help of another mentor 
or organization, to think about (histology-related) projects for science fairs 
leading to a state fair and Intel ISEF.  Can't think of any better way to 
"promote histology" so would hope those at NSH would take note of this.  And 
since the ISEF fair receives projects and groups from 70 countries, I hope any 
outside the US would also think about the same thing. 
  
Ray Koelling 
HT, HTL, QIHC, STEM educational outreach advocate 
Lake Forest Park, WA 
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Re: [Histonet] Nuclear "Artifact"

[Michael Ann Jones]

Happy Lab Week!!
We worked on our H&E for almost two years. We were using Leica H&E
products and after 2 years of struggling, adjusting and analyzing
everything in our lab - we switched to the Richard-Allen Scientific
products. We were seeing variability between days of staining, tissues
right next to each other, nuclear paleness, eosin uniformity instead of
differentiated, etc. Switching reagents helped us tremendously - we have
more consistent higher quality stains on a daily basis and within tissues.

I you¹re struggling and have analyzed your processors, etc. to death -
maybe try different reagents? (we even measured the tap water that we use
on our stainer daily, the pH of reagents every other hour etc. and between
5 experienced histotechs, we couldn¹t figure it out)
Good luck! :)

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com







On 4/21/15, 5:55 PM, "Sue"  wrote:

>OMG we are experiencing the same issue. At first it was just GI and now
>we are seeing it on prostate. One pathologist said it looks like the
>tissue has been cooked. The only issue is we can have two biopsies right
>next to one another in the basket one looks good and one looks bad. My
>director also thinks it is the processors. I had Thermo out and they
>could find nothing. We changed out all the reagents and the biopsies were
>fine than two days later we had some bad ones. I know in July Fisher had
>a formalin recall associated to the mixture of buffer, water and
>formalin. We thought that might be it but it is now almost a year later
>and all the bad formalin should be gone. The histotechs say the tissue is
>crunchy and they are right. I am running a test tonight of a small needle
>biopsy that I made from a colon. I placed it is straight formaldehyde
>overnight and am processing it on our biopsy cycle tonight. My director
>also wanted us to only put three levels on our Thermo, but he wanted the
>middle level to have empty baskets. I stopped that today because I think
>the other issue is that the poor biopsies may be on the top level and as
>the reagents are used the level changes, and also due to displacement
>with the middle level being empty the reagent levels may not reach the
>top. We just do not have the manpower to inspect every reagent every day,
>we have 6 processor and it would take a tech all day. We actually take a
>digital picture when they come out of the processor. I want to check my
>problems cases tomorrow. We do not use sponges but the only other like
>was the PA who was wrapping the blue paper very tight around the tissue.
>I really do not think this is the issue though.. Any other insight would
>be greatly appreciated.
>
>Susan T. Paturzo 
>TJUH 
>___
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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