Re: [Histonet] Hematoxylin Precipitate and filtering Gill formulations
Yes, I have used Gill 1, 2 and 3 even in the early days of buying these formulations from a vendor, and always filtered them before using. Old school habits never changed.. Gayle Callis -Original Message- From: Manfre, Philip via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 12:26 PM To: Elizabeth Chlipala ; Tim H Cc: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Hematoxylin Precipitate Wow, I agree with Liz. There should not routinely be "so much tissue washing off". There is a fundamental problem, if this is the case. With regards to hematoxylin, have you tried Gill's Hematoxylin, 1, 2, or 3? These do not need filtering and do not produce a precipitate. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Quick H202 quenching question.
If I have an IHC where I am staining 2 slides from the same block, one with one primary antibody and the other with a different primary antibody. And one antibody's slides have high nonspecific background, but the other antibody's slides have no background, Can I deduce that the background staining is not caused by insufficient H202 quenching/blocking? All the steps were the same, except for the primary antibody/secondary antibody. Also, they did have different epitope retrievals. But the wash buffers/blocking buffers/and substrate were the same. I am fairly confident that my background problems are related to this particular primary antibody, and not the actual quenching/blocknig from my IHC protocol, but I thought I'd check with you guys. Patrick. Patrick Lewis Research Associate II Bench Seattle Childrens Research Institute 206-884-1115 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hematoxylin Precipitate
Tim First of all my comment was not meant to criticize your post and even if you may not think so I was trying to help. I stand by what I said, my comment was to address what I thought was the amount of tissue loss your lab experiences and if I took your comment too literally I apologize but I firmly believe that properly processed and sectioned tissue samples should remain on the slide. I do understand that we will on occasion have loss of tissue from the slides that we cut and stain. We will see a lens floating in one of the alcohols when we stain mouse eyes or a portion of a dermal construct will come off the slides if we have not dried it properly. Most of the tissue loss we experience is due to improperly processed and sectioned samples. We are a small lab and we are GLP compliant. We do not change our H&E staining reagents daily our volume varies depending upon the projects we are working on, but I can tell you that we have looked at H&E staining over time and we have made sure that our reagents are changed appropriately. We do not filter our hematoxylin daily and have not experienced many floaters or carry over or other things on our slides. Liz from wacky tabacky country Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Tim H via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 3:04 PM To: gayle.cal...@bresnan.net; Histonet Subject: Re: [Histonet] Hematoxylin Precipitate Liz, Phillip and all that are interested, I take it you have guys never looked at or had someone else examine what is at the bottom of the Hematoxylin filter after you put through a day's work. There will be tissue particles of tissue along with other contaminates, I am not saying you are going to see a complete LEEP sitting at the bottom but you will have contamination. Liz, maybe the tissue super adheres up there in wacky tabacky country and Phillip sitting in a research facility (are you even involved with processing and staining of slides or just publishing articles?) but in the rest of the United States, I can guarantee you there will be some tissue in the Hematoxylin if you use a traditional dip and dunk system. Liz, you do bring up a good point about having tissue in every container. To a degree you will have tissue in every reagent container. I was assuming and maybe unjustly that most labs are using Good Laboratory Practice and discard their reagents after they have used them for a staining session. This topic was about Hematoxylin Precipitate and "small spore or pollen-like blue dots" which I did not say was tissue, I was passing along some of my 23 years of knowledge. Listen; don't take my word for it. You can have Ventana come out to your facility and filter all your reagents and stains and have them tell you what is in your solutions. Next time just pass along good information and criticize those trying to help! To many people like to make a negative on this site of people trying to truly help. This is why I rarely post on Histonet but instead directly email the person. By no means am I promoting Ventana/Roche products. I am just passing along information that might be helpful. Tim Disclaimer: This information is by no means is meant for any weak stomach individuals or those preparing for the zombie apocalypse. > From: gayle.cal...@bresnan.net > To: thiggin...@msn.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Hematoxylin Precipitate > Date: Tue, 22 Sep 2015 12:27:21 -0600 > > Sandy, > > After years of using Richard Allan's hematoxylin 2 with great success, if > we didn't filter daily before use, we had stain precipitate on sections. > Some of this comes from the hematoxylin continuing to oxidize in open air, > bacteria and other "crud". Tim is absolutely correct ignoring > manufacturers no filtering instructions. Being old school, we were taught > to faithfully filter any hematoxylin, regardless of progressive or > regressive types.If we topped off hematoxylin 2 or used new stock, the > stain was filtered into a CLEAN staining container/dish. Keep an extra > container around if possible. We used a medium fast filter paper, Whatman > 54. I realize this takes time but junk on a slide is NOT good thing, > especially after IHC staining and have a photo to show this - the result of > being lazy and not filtering the hematoxylin on that particular day. > > We used a distilled water rinse before hematoxylin2, but DI H2O will > be contaminated with cellular debris and last hydrating alcohol carryover. > Change DI water frequently if you have many runs in a day. We used 1 > minute running tap water ri
[Histonet] Elastic Stain on the Benchmark Special Stainer (BMSS)?
Hi Netters, Has anyone out in Histoland achieved blackness with the BMSS Elastic stain? All I'm getting are purple elastic fibers. If you have crossed over to the darker side of staining and have a protocol you'd be willing to share, by all means let me know. I want to join the darkside too! At least with the Elastic stain. Thanks in advance, Paula :-) UCSD Health System ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hematoxylin Precipitate
Liz, Phillip and all that are interested, I take it you have guys never looked at or had someone else examine what is at the bottom of the Hematoxylin filter after you put through a day’s work. There will be tissue particles of tissue along with other contaminates, I am not saying you are going to see a complete LEEP sitting at the bottom but you will have contamination. Liz, maybe the tissue super adheres up there in wacky tabacky country and Phillip sitting in a research facility (are you even involved with processing and staining of slides or just publishing articles?) but in the rest of the United States, I can guarantee you there will be some tissue in the Hematoxylin if you use a traditional dip and dunk system. Liz, you do bring up a good point about having tissue in every container. To a degree you will have tissue in every reagent container. I was assuming and maybe unjustly that most labs are using Good Laboratory Practice and discard their reagents after they have used them for a staining session. This topic was about Hematoxylin Precipitate and "small spore or pollen-like blue dots" which I did not say was tissue, I was passing along some of my 23 years of knowledge. Listen; don't take my word for it. You can have Ventana come out to your facility and filter all your reagents and stains and have them tell you what is in your solutions. Next time just pass along good information and criticize those trying to help! To many people like to make a negative on this site of people trying to truly help. This is why I rarely post on Histonet but instead directly email the person. By no means am I promoting Ventana/Roche products. I am just passing along information that might be helpful. Tim Disclaimer: This information is by no means is meant for any weak stomach individuals or those preparing for the zombie apocalypse. > From: gayle.cal...@bresnan.net > To: thiggin...@msn.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Hematoxylin Precipitate > Date: Tue, 22 Sep 2015 12:27:21 -0600 > > Sandy, > > After years of using Richard Allan's hematoxylin 2 with great success, if > we didn't filter daily before use, we had stain precipitate on sections. > Some of this comes from the hematoxylin continuing to oxidize in open air, > bacteria and other "crud". Tim is absolutely correct ignoring > manufacturers no filtering instructions. Being old school, we were taught > to faithfully filter any hematoxylin, regardless of progressive or > regressive types.If we topped off hematoxylin 2 or used new stock, the > stain was filtered into a CLEAN staining container/dish. Keep an extra > container around if possible. We used a medium fast filter paper, Whatman > 54. I realize this takes time but junk on a slide is NOT good thing, > especially after IHC staining and have a photo to show this - the result of > being lazy and not filtering the hematoxylin on that particular day. > > We used a distilled water rinse before hematoxylin2, but DI H2O will be > contaminated with cellular debris and last hydrating alcohol carryover. > Change DI water frequently if you have many runs in a day. We used 1 > minute running tap water rinses after hematoxylin, clearant and bluing. If > you are using running water rinses, take a look at the blue ppt in the post > hematoxylin container as you don't want that sticking to sections. Non > running water rinses should be changed after each H&E run in my opinion. > Adequate clean water rinses are important to not have carry over of clearant > into bluing reagents or bluing reagent into eosin in order to maintain > correct pH for staining. > > Good luck > > Gayle M. Callis > HTL/HT/MT(ASCP) > > -Original Message- > From: Tim H via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Tuesday, September 22, 2015 11:25 AM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Hematoxylin Precipitate > > You should be filtering your Hematoxylin on a daily basis regardless of what > the manufactures says. We use to filter twice a day since we did a > traditional overnight run and then again in the afternoon for specimens that > had been microwave processed. So much tissue washes off in the solutions > they should be changed or filtered fairly regularly to try and prevent cross > contamination on the slides. > > You can also try increasing your rinse times and see if that doesn't help as > well. > > Thanks, > > Tim > > > > Message: 1 > > Date: Mon, 21 Sep 2015 15:14:39 -0500 > > From: "Sandra Cheasty" > > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > > > Subject: [Histonet] Hematoxylin Precipitate > > Message-ID: <4cda87133587e64c965ce6c356d18...@svm.vetmed.wisc.edu> > > Content-Type: text/plain; charset="us-ascii" > > > > Hello all, > > > > Has anyone using Richard Allen Hematoxylin-2 noticed an > odd artifact on the slides after using th
Re: [Histonet] Webinar presentation hand-outs
Elaine you can download the powerpoint and all the handouts from the Advance for Laboratories website under Webinars, QA/QC, " Basic Principles of Validation in Histology" Link is below: http://laboratory-manager.advanceweb.com/Webinar/Editorial-Webinars/Basic-Principles-of-Validation-in-Histology.aspx I hope you find it helpful. Contact me directly with any questions or comments. Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Elaine allison Hoffman via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:45 AM To: Histonet List Subject: [Histonet] Webinar presentation hand-outs Hi all, Just recently joined in with an Advance webinar last week, 9/15/15, Tuesday with speaker, Tim Morken on "Basic Principles of Validation in Histology". The webinar was very informative and thorough. The power-point presentation looked to be about 40 pages long. I'm interested in getting a copy of the the presentation shown during the webinar but don't know how. There was definitely too much to remember and our lab could really use it ASAP. Wasn't able to write it down fast enough. I left emails with Advance but no answer back yet. Any suggestions?? Appreciate any help, thanks Elaine Hoffman, HT(ASCP) The Gastroenterology Clinic & Endoscopy Center, Inc.GI Pathology Associates ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Webinar presentation hand-outs
Hi all, Just recently joined in with an Advance webinar last week, 9/15/15, Tuesday with speaker, Tim Morken on "Basic Principles of Validation in Histology". The webinar was very informative and thorough. The power-point presentation looked to be about 40 pages long. I'm interested in getting a copy of the the presentation shown during the webinar but don't know how. There was definitely too much to remember and our lab could really use it ASAP. Wasn't able to write it down fast enough. I left emails with Advance but no answer back yet. Any suggestions?? Appreciate any help, thanks Elaine Hoffman, HT(ASCP) The Gastroenterology Clinic & Endoscopy Center, Inc.GI Pathology Associates ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hematoxylin Precipitate
Sandy, After years of using Richard Allan's hematoxylin 2 with great success, if we didn't filter daily before use, we had stain precipitate on sections. Some of this comes from the hematoxylin continuing to oxidize in open air, bacteria and other "crud". Tim is absolutely correct ignoring manufacturers no filtering instructions. Being old school, we were taught to faithfully filter any hematoxylin, regardless of progressive or regressive types.If we topped off hematoxylin 2 or used new stock, the stain was filtered into a CLEAN staining container/dish. Keep an extra container around if possible. We used a medium fast filter paper, Whatman 54. I realize this takes time but junk on a slide is NOT good thing, especially after IHC staining and have a photo to show this - the result of being lazy and not filtering the hematoxylin on that particular day. We used a distilled water rinse before hematoxylin2, but DI H2O will be contaminated with cellular debris and last hydrating alcohol carryover. Change DI water frequently if you have many runs in a day. We used 1 minute running tap water rinses after hematoxylin, clearant and bluing. If you are using running water rinses, take a look at the blue ppt in the post hematoxylin container as you don't want that sticking to sections. Non running water rinses should be changed after each H&E run in my opinion. Adequate clean water rinses are important to not have carry over of clearant into bluing reagents or bluing reagent into eosin in order to maintain correct pH for staining. Good luck Gayle M. Callis HTL/HT/MT(ASCP) -Original Message- From: Tim H via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18...@svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd artifact on the slides after using the Hematoxylin for more than a few days on their stainer? We are seeing small spore or pollen-like blue dots here and there on the slides. It is not coming from the water bath or our water supply on the stainer. I used sterile gloves, opened a new case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI again, air-dried and coverslipped them, and the blue dots were there. The only way we got rid of the blue artifact was to use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hematoxylin Precipitate
Wow, I agree with Liz. There should not routinely be "so much tissue washing off". There is a fundamental problem, if this is the case. With regards to hematoxylin, have you tried Gill's Hematoxylin, 1, 2, or 3? These do not need filtering and do not produce a precipitate. Phil. Philip Manfre, B.A., HT (ASCP) Associate Principal Scientist Merck Research Laboratories WP45-251 PO Box 4 West Point, PA 19486 215-652-9750 215-993-0383 (fax) philip_man...@merck.com -Original Message- From: Elizabeth Chlipala via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 1:55 PM To: Tim H; 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) Subject: Re: [Histonet] Hematoxylin Precipitate I think you may a have a serious problem if "so much tissue washes off" if that is happening you have problems with tissue adherence. A properly processed and cut section should not wash off the slide, or even a portion of it should not wash off. That would mean that you did not provide to the pathologist what is represented in the block. If that is the case then you would need to filter all solutions on a stainer daily not just the hematoxylin. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Tim H via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18...@svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd > artifact on the slides after using the Hematoxylin for more than a few days > on their stainer? We are seeing small spore or pollen-like blue dots here and > there on the slides. It is not coming from the water bath or our water supply > on the stainer. I used sterile gloves, opened a new case of slides, dipped > them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI > again, air-dried and coverslipped them, and the blue dots were there. The > only way we got rid of the blue artifact was to use new RA Hematoxylin-2 > every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, > reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hematoxylin Precipitate
I think you may a have a serious problem if "so much tissue washes off" if that is happening you have problems with tissue adherence. A properly processed and cut section should not wash off the slide, or even a portion of it should not wash off. That would mean that you did not provide to the pathologist what is represented in the block. If that is the case then you would need to filter all solutions on a stainer daily not just the hematoxylin. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Tim H via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18...@svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd > artifact on the slides after using the Hematoxylin for more than a few days > on their stainer? We are seeing small spore or pollen-like blue dots here and > there on the slides. It is not coming from the water bath or our water supply > on the stainer. I used sterile gloves, opened a new case of slides, dipped > them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI > again, air-dried and coverslipped them, and the blue dots were there. The > only way we got rid of the blue artifact was to use new RA Hematoxylin-2 > every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, > reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hematoxylin Precipitate
You should be filtering your Hematoxylin on a daily basis regardless of what the manufactures says. We use to filter twice a day since we did a traditional overnight run and then again in the afternoon for specimens that had been microwave processed. So much tissue washes off in the solutions they should be changed or filtered fairly regularly to try and prevent cross contamination on the slides. You can also try increasing your rinse times and see if that doesn't help as well. Thanks, Tim > > Message: 1 > Date: Mon, 21 Sep 2015 15:14:39 -0500 > From: "Sandra Cheasty" > To: "Histonet (histonet@lists.utsouthwestern.edu)" > > Subject: [Histonet] Hematoxylin Precipitate > Message-ID: <4cda87133587e64c965ce6c356d18...@svm.vetmed.wisc.edu> > Content-Type: text/plain; charset="us-ascii" > > Hello all, > > Has anyone using Richard Allen Hematoxylin-2 noticed an odd > artifact on the slides after using the Hematoxylin for more than a few days > on their stainer? We are seeing small spore or pollen-like blue dots here and > there on the slides. It is not coming from the water bath or our water supply > on the stainer. I used sterile gloves, opened a new case of slides, dipped > them in DI water, then in the RA Hematoxylin 2 on the stainer, then in DI > again, air-dried and coverslipped them, and the blue dots were there. The > only way we got rid of the blue artifact was to use new RA Hematoxylin-2 > every 2-3 days, which is a bit expensive. > > Thanks for your input, and if you can recommend a different, > reasonably priced hematoxylin, that would be awesome. > > Cheers, > > Sandy > > > > Sandra J. Cheasty, HT (ASCP) > > Histology & Necropsy Supervisor > > UW-Madison, School of Veterinary Medicine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hematoxylin Precipitate
Denatured alcohol is used all the time. It is generally denatured so people don't drink it. This stems back to a time when the histology lab also made for a great bar and grill. :-) Phil. -Original Message- From: Alida Bailleul via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 22, 2015 11:28 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin Precipitate Dear Histonetters, There is some *Denatured Ethanol* bottles in the lab I just started working in (CDA, Fisher brand). Can this denatured ethanol be used for histological processing? I have never used this product, only absolute pure ethanol. Please advise. Thank you very much All the best Alida Bailleul ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth, New Jersey, USA 07033), and/or its affiliates Direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How deep in skin do hair dyes and shampoos penetrate?
Dear Histonetters: The other day, someone ask me in class, how deep in skin do hair dyes and shampoos penetrate? I know tattoos are generally placed in the dermis but I was not sure re. hair dyes and shampoos (incl. those that cause a tingling sensation). If you know, please feel free to email me off the list. Gratefully, Jorge blayjo...@gmail.com Jorge A. Santiago-Blay, PhD blaypublishers.com 1. Positive experiences for authors of papers published in *LEB* http://blaypublishers.com/testimonials/ 2. Free examples of papers published in *LEB*: http://blaypublishers.com/category/previous-issues/. 3. *Guidelines for Authors* and page charges of *LEB*: http://blaypublishers.com/archives/ *.* 4. Want to subscribe to *LEB*? http://blaypublishers.com/subscriptions/ http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.cfm ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hematoxylin Precipitate
Dear Histonetters, There is some *Denatured Ethanol* bottles in the lab I just started working in (CDA, Fisher brand). Can this denatured ethanol be used for histological processing? I have never used this product, only absolute pure ethanol. Please advise. Thank you very much All the best Alida Bailleul ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Hematoxylin Precipitate
Yes, me too. Turquois blue bacteria looking guys. Trying to filter and use within a couple of days. Has anyone mentioned this to their vendor? Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 9/21/15, 3:17 PM, "nancy lowen via Histonet" wrote: >Yes, I have had the same experience.Filtering it seems to help. > > > On Monday, September 21, 2015 1:22 PM, Sandra Cheasty via Histonet > wrote: > > > Hello all, > >Has anyone using Richard Allen Hematoxylin-2 noticed an >odd artifact on the slides after using the Hematoxylin for more than a >few days on their stainer? We are seeing small spore or pollen-like blue >dots here and there on the slides. It is not coming from the water bath >or our water supply on the stainer. I used sterile gloves, opened a new >case of slides, dipped them in DI water, then in the RA Hematoxylin 2 on >the stainer, then in DI again, air-dried and coverslipped them, and the >blue dots were there. The only way we got rid of the blue artifact was to >use new RA Hematoxylin-2 every 2-3 days, which is a bit expensive. > >Thanks for your input, and if you can recommend a >different, reasonably priced hematoxylin, that would be awesome. > >Cheers, > >Sandy > > > >Sandra J. Cheasty, HT (ASCP) > >Histology & Necropsy Supervisor > >UW-Madison, School of Veterinary Medicine > > > > > > > > > > > >___ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >___ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
