Re: [Histonet] Blog Post--not work related

2015-12-17 Thread Rene J Buesa via Histonet
Why do you keep posting "not work related" things?I do not think this is 
proper, even if you think they are.It is annoying having to "spam" your 
postings.René 

On Thursday, December 17, 2015 1:23 PM, Lester Raff MD via Histonet 
 wrote:
 

 We are making progress, but doesn't look like I am going to give up my day job.

http://www.chicagonow.com/downsize-maybe/2015/12/if-we-spend-it-they-will-come/

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] Blog Post--not work related

2015-12-17 Thread Lester Raff MD via Histonet
We are making progress, but doesn't look like I am going to give up my day job.

http://www.chicagonow.com/downsize-maybe/2015/12/if-we-spend-it-they-will-come/

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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Re: [Histonet] Expiration dates of ammonium sulphide

2015-12-17 Thread Morken, Timothy via Histonet

Hi Karen, 

We get ours from Sigma-aldrich:
Ammonium Sulfide, 25ml  A1952-25Sigma-Aldrich   $34.12  EA  25 ml   

This is a 20% solution
We just got some so I know they have it.

We use a 10% solution of this, so it comes out to 2% final concentration: we 
use 4ml of the ammonium sulfide we buy in 36ml water. 

I don't remember the posting but in practical use ammonium sulfide is easily 
added in excess of the need for the reaction, so in that sense concentration 
does not matter much. But maybe if you made it super low concentration it would 
not work as well or as fast. For our purposes 2% final concentration and up is 
fine. 


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center



-Original Message-
From: Bryan, Karen via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, December 16, 2015 1:19 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Expiration dates of ammonium sulphide

Dear Mr. Morken,

I found your thread on Histonet regarding ammonium sulfide for muscles. We are 
running a panel including ATPase every week and have been having problems with 
the manufacturer with the supply. As of today we were told by FISHER that they 
are back ordered indefinitely.

What company are you using to buy the 25 ml bottle? I also see you stated back 
in 2010 that the concentration doesn't matter, but just so you have the 
information we are using 21% aqueous ammonium sulfide light (as stated on the 
bottle).

I hope you are still at this email address!

Thank you in advance for any help you can provide.

Karen

Karen P. Bryan, HT (ASCP)cm
Sr. Neurohistology Specialist
Department of Pathology/Amory 3
Brigham & Women's Hospital
75 Francis Street/Boston
617-732-7534
(Internal extension 2-7534)




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Re: [Histonet] H.pylori Control Blocks

2015-12-17 Thread Hist10 via Histonet
Histo controls they are part of poly scientific they had every type of control





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On Tuesday, December 15, 2015, Helene Degan via Histonet 
 wrote:

Morning We are in search of H.pylori control blocks, any one with information 
as to where we can purchase them I would appreciate it. Thanks Helene Degan 
Upstate Medical University Syracuse,NY 
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Re: [Histonet] Cell block protocol

2015-12-17 Thread Michael Ann Jones via Histonet
We use HistoGel instead of Cellblock with 100% capture of the specimen
(validated) and no peeling or sliding off of slides.
Hope that helps.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com



On 12/17/15, 7:16 AM, "Charles Riley via Histonet"
 wrote:

>Does anyone have a cell block protocol they use for Ultra sound Fine
>needle
>aspirates? We have been experiencing some issues with the tissue falling
>off slides and washing off immunostains. Any help would be greatly
>appreciated
>
>-- 
>
>Charles Riley HT(ASCP)CM
>
>Histopathology Coordinator/ Mohs
>
>Doctors Pathology Services, Dover DE
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[Histonet] Cell block protocol

2015-12-17 Thread Charles Riley via Histonet
Does anyone have a cell block protocol they use for Ultra sound Fine needle
aspirates? We have been experiencing some issues with the tissue falling
off slides and washing off immunostains. Any help would be greatly
appreciated

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs

Doctors Pathology Services, Dover DE
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[Histonet] cellient cell block usage

2015-12-17 Thread Melissa Likens via Histonet
Was just wondering if anyone is using the Hologic's Cellient automated cell 
block equipment and if you are happy with results?  Does anyone using this 
technology use Venatana Immunostains on these blocks with any success?  Any 
info is greatly appreciated.  ]
Thank you, Melissa L
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