Re: [Histonet] Cryostat Chirp

[Elizabeth Chlipala via Histonet]

Gary

I think that might have to do with the fan motor inside the unit, it might need 
to be replaced.   We had ours replaced a few years ago.  Do you service/PM the 
cryostat yearly?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Martin, Gary via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, May 03, 2016 2:46 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Chirp

My Leica CM 1850 cryostat is making a chirping sound and I can't figure out 
what is doing this. Has anyone experienced this sound, if what was the resolve. 

Thanks 

Gary

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[Histonet] Cryostat Chirp

[Martin, Gary via Histonet]

My Leica CM 1850 cryostat is making a chirping sound and I can't figure
out what is doing this. Has anyone experienced this sound, if what was
the resolve. 

Thanks 

Gary

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Re: [Histonet] PAS/Decal Question

[Rene J Buesa via Histonet]

My impression is that your problem is during the decalcification step. It 
cannot be hurried and has to be in EDTA at pH 7All reagents have to be prepared 
in pH7 phosphate buffer.The inconsistency resides in the fact that not all core 
Bx are the same regarding thickness, tissue condition or size.Besides you are 
hurrying too much. As yourself (and your pathologists) the following question: 
what good you take out of your protocol if the "failure" rate is as big as you 
describe?Change to EDTA and process more time.René 

On Tuesday, May 3, 2016 1:01 PM, "Marcum, Pamela A via Histonet" 
 wrote:
 

 We are still having issues with our PAS stain on decaled bone marrows.  The 
Pathologists in HemePath are seeing what they refer to as smudginess in cells 
on some areas of the completed PAS slides.  We have looked at everything and 
cannot find where the issue is coming from at this point.  We have done manual 
staining for PAS, automated on the Leica stainer and on the Dako Artisan.  All 
methods show the same result for some slides.  We can go for several days to a 
week or more with no problem and then suddenly it is back and we have changed 
nothing in the way we do the processing, embedding, sectioning, 
deparaffinization and coverslipping.  We do as many as 38 bone marrow cores a 
night or as few as 8 and can find no correlation in the number we have to deal 
with for a given period.  All bone marrows drawn today must be completed by 8AM 
tomorrow morning.

Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a 
maximum of 7 hours +/-.

Grossed and placed in cassettes for 15 minute rinse in running DI Water

Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C

Rinsed in running DI water for 15 minutes

Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours 
minimum to come off at 4:45AM.

If anyone knows of any literature on decal effects on PAS staining in bone 
marrows please contact me.  This has been going on for months and no matter 
what we do manual staining, Leica adaptation for automated or Dako it is not 
helping.  Dako has been great with sending in technical experts repeatedly and 
we cannot get this corrected.

Thanks,
Pam

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Re: [Histonet] Histonet Digest, Vol 150, Issue 2

[T H via Histonet]

Good to know that I am not the only on that think Rene is negative to others.  
Hey Rene it's not just one person that thinks your a dirt bag.


From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Tuesday, May 3, 2016 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 150, Issue 2

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."


Today's Topics:

   1. No More Blog Posts --  Over and Out! (Lester Raff MD)
   2. IHC positive controls (Cindy Bulmer)
   3. Re: No More Blog Posts --  Over and Out! (Rene J Buesa)
   4. Fwd: (Linda Margraf)
   5. Re: No More Blog Posts --  Over and Out! (Manfre, Philip)
   6. Re: IHC positive controls (Morken, Timothy)
   7. Re: No More Blog Posts --  Over and Out! (Boyd, Debbie M)
   8. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
   9. Re: No More Blog Posts -- Over and Out! (Caroline Miller)
  10. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
  11. Re: Fwd: (Rene J Buesa)
  12. HHV-8 RTU (Delray Beach Pathology Kari Simeone)
  13. PAS/Decal Question (Marcum, Pamela A)


--

Message: 1
Date: Mon, 2 May 2016 17:39:28 +
From: Lester Raff MD 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] No More Blog Posts --  Over and Out!
Message-ID:
<6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local>

Content-Type: text/plain; charset="us-ascii"

To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com   The mailing list is never used 
for any purpose other than announcing a new blog post. Be sure to let me know 
you are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203



--

Message: 2
Date: Mon, 2 May 2016 13:42:36 -0400
From: Cindy Bulmer 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls
Message-ID: <15472908bba-2169-b...@webprd-a99.mail.aol.com>
Content-Type: text/plain; charset=utf-8


Hello Histoland,

I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?

1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) for 
1 hr.
 then put slides in refrigerator for future use.
2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and put 
slides
 directly in refrigerator for future use.
3)  Cut "fresh" every time they order the Ab.

Thank you,
Cindy


Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX






--

Message: 3
Date: Mon, 2 May 2016 18:22:59 + (UTC)
From: Rene J Buesa 
To: Lester Raff MD ,
"'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] No More Blog Posts --  Over and Out!
Message-ID:
<1946825210.5059374.1462213379630.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

Thank you VERY MUCH!Ren?

On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet 
 wrote:


 To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.? Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com? The mailing list is never used for 
any purpose other than announcing a new blog post. Be sure to let me know you 
are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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[Histonet] Golgi Kopsch

[Mca Werdler via Histonet]

Hello Everyone,

I am completely new to the technique, i tried several different protocols
but it doesn't seemed to succeed that very well. Does anyone have a
protocol for the Golgi Kopsch, where i have to include the tissue in the
end with paraffin?

Thank you all for your time

Mwerdler

UNAM Mexico
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[Histonet] No More Blog Posts -- Over and Out!

[Blake Taylor via Histonet]

I have been completely disappointed at the remarks and ugliness that I have 
seen lately on this post.  I will be extremely unlikely to continue to use this 
list serve anymore due to the complete lack of professionalism and compassion 
that has been displayed of late. This blog post talk has certainly brought out 
the worst in people but it certainly is not the first time I've seen people be 
short, judgmental and rude on this site.  It has been nice to see some people 
speak up and ask others to behave better but as usual it is the worst of the 
crowd that always seems to be the loudest.

Blake Taylor
Surgical Pathology

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Tuesday, May 03, 2016 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 150, Issue 2

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


Today's Topics:

   1. No More Blog Posts --  Over and Out! (Lester Raff MD)
   2. IHC positive controls (Cindy Bulmer)
   3. Re: No More Blog Posts --  Over and Out! (Rene J Buesa)
   4. Fwd: (Linda Margraf)
   5. Re: No More Blog Posts --  Over and Out! (Manfre, Philip)
   6. Re: IHC positive controls (Morken, Timothy)
   7. Re: No More Blog Posts --  Over and Out! (Boyd, Debbie M)
   8. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
   9. Re: No More Blog Posts -- Over and Out! (Caroline Miller)
  10. Re: No More Blog Posts --  Over and Out! (WILLIAM DESALVO)
  11. Re: Fwd: (Rene J Buesa)
  12. HHV-8 RTU (Delray Beach Pathology Kari Simeone)
  13. PAS/Decal Question (Marcum, Pamela A)


--

Message: 1
Date: Mon, 2 May 2016 17:39:28 +
From: Lester Raff MD 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] No More Blog Posts --  Over and Out!
Message-ID:
<6347C6D2B080534F9B5C2B08436DCFAF10D5E354@COLOEXCH01.uropartners.local>

Content-Type: text/plain; charset="us-ascii"

To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.  Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com   The mailing list is never used 
for any purpose other than announcing a new blog post. Be sure to let me know 
you are from the Histonet list!

I will continue to participate in any histology/pathologist discussions here as 
I have for many years.

Cheers,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203



--

Message: 2
Date: Mon, 2 May 2016 13:42:36 -0400
From: Cindy Bulmer 
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC positive controls
Message-ID: <15472908bba-2169-b...@webprd-a99.mail.aol.com>
Content-Type: text/plain; charset=utf-8


Hello Histoland,
 
I need some advice, I have a PT block that is positive with Spirochetes.
What would be the best way to use this block as a positive control?
 
1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) for 
1 hr. 
 then put slides in refrigerator for future use.
2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and put 
slides
 directly in refrigerator for future use.
3)  Cut "fresh" every time they order the Ab.
 
Thank you,
Cindy


Cynthia Bulmer HT(ASCP),QIHC
IHC Supervisor, CTPL
Waco, TX






--

Message: 3
Date: Mon, 2 May 2016 18:22:59 + (UTC)
From: Rene J Buesa 
To: Lester Raff MD ,
"'histonet@lists.utsouthwestern.edu'"

Subject: Re: [Histonet] No More Blog Posts --  Over and Out!
Message-ID:
<1946825210.5059374.1462213379630.javamail.ya...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

Thank you VERY MUCH!Ren? 

On Monday, May 2, 2016 1:54 PM, Lester Raff MD via Histonet 
 wrote:
 

 To My Lab Colleagues:

As my intent has never been to sow discontent or rancor, I think it is for the 
best if I no longer post links to my blog, lab related or otherwise.? Of course 
the blogs go on, and if anyone is interested in being added to my mailing list 
for future notifications, just drop me a line at 
les.r...@post.com?

[Histonet] Job Opening in CA

[Paula Keene Pierce via Histonet]

| 
|  |

 |


| 
| Dear Friends & Family,I am seeking a Research Scientist I, Stem Cell Biology 
for a stem cell therapy project that we just started. The funding has been 
secured for the entire project. The position is available immediately and the 
description is shown below. If you know somebody who may be interested in this 
opening, please share this e-mail with your contact. Any help, advice or 
recommendations are highly appreciated as always!Respectfully,Nikolay Turovets, 
Ph.D.CEO MediCell Technologies, LLC |

 |


| 
| 
| 
|  |

 |

 |

 |


| 
| RESEARCH SCIENTIST I, Stem Cell Biology |

 |


| 
| Job Description
Novel exclusive stem cell therapy project is seeking a highly motivated 
scientist to join its team.In consortium with a leading hospital in Los Angeles 
and a recognized business partner, MediCell Technologies is developing a novel 
stem cell-based therapeutic platform. The technology has been validated in 
animal proof-of-concept studies and attracts interests of key opinion leaders 
of the field. The funding has been secured for entire project.We are looking 
for a highly motivated and pro-active individual who is willing to grow with 
the project and contribute to the development of stem cell-based therapies. Our 
project is an outstanding opportunity for the right individual to display their 
talents and establish themselves in this highly competitive field.The scientist 
will be responsible for the derivation, expansion and characterization of a 
unique type of human stem cells. He or She will be involved in all range of 
activities associated with the technology transfer to a GMP facility as well as 
preparing a portfolio of Standard Operation Procedures. The Scientist will play 
an integral role in managing various aspects of lab operations-associated 
activities. The work will require periodic work on weekends, holidays and after 
business-hours time.This position requires hard work, ingenuity, and a strong 
commitment to a productive work environment. If you are not a highly confident, 
self-starting individual, this position is not meant for you.
 |

 |


| 
| Required qualifications   
   - PhD in cell biology, stem cell biology, tissue engineering or related 
discipline. 3+ years of work experience in stem cell research.
   - Outstanding hands-on techniques in stem cell culture, cell-based assays 
(including ICC, qPCR, FACS), and cell culture process development.
   - Excellent English written and oral communication skills, able to write 
SOPs, technical reports and protocols.
   - Demonstrated excellence in planning, executing, and analyzing experiments.
   - Able to troubleshoot, solve difficult problems, and develop process 
improvements.
   - Attention to detail and careful record-keeping.
   - Excellent organizational skills and ability to manage multiple projects.
   - Flexible team player excited to collaborate with internal and external 
partners.
   - Highly self-motivated.
   - Flexibility in working schedule to accommodate weekend/holiday and 
out-business hours work if necessary.
   - Authorized to legally work in US.
Preferred (but not required) qualifications:   
   - Experience with human pluripotent and/or mesenchymal stem cells is 
preferred.
   - Experience to work in GMP and/or GLP environment is appreciated.

Salary: up to $85,000/annual + health insuranceJob Location: Carlsbad, 
CARe-location expense: Not offeredEmployment term: Full time, 3 month probation 
period |

 |


| 
| 
| 
|  |

 |

 |

 |


| 
| HOW TO APPLYPlease send CV and Cover Letter to Nikolay Turovets, PhD: 
n...@medicelltech.com |

 |

 Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 
405-759-7513www.excaliburpathology.com
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[Histonet] PAS/Decal Question

[Marcum, Pamela A via Histonet]

We are still having issues with our PAS stain on decaled bone marrows.  The 
Pathologists in HemePath are seeing what they refer to as smudginess in cells 
on some areas of the completed PAS slides.  We have looked at everything and 
cannot find where the issue is coming from at this point.  We have done manual 
staining for PAS, automated on the Leica stainer and on the Dako Artisan.  All 
methods show the same result for some slides.  We can go for several days to a 
week or more with no problem and then suddenly it is back and we have changed 
nothing in the way we do the processing, embedding, sectioning, 
deparaffinization and coverslipping.  We do as many as 38 bone marrow cores a 
night or as few as 8 and can find no correlation in the number we have to deal 
with for a given period.  All bone marrows drawn today must be completed by 8AM 
tomorrow morning.

Fixation after pulling the bone marrows is a minimum of 2 hours in AZF with a 
maximum of 7 hours +/-.

Grossed and placed in cassettes for 15 minute rinse in running DI Water

Decal currently in the Milestone Decal Unit for 45 minutes in Immunocal at 50C

Rinsed in running DI water for 15 minutes

Placed in 10% NBF and processed on a 4 hour program with a delay of 4 hours 
minimum to come off at 4:45AM.

If anyone knows of any literature on decal effects on PAS staining in bone 
marrows please contact me.  This has been going on for months and no matter 
what we do manual staining, Leica adaptation for automated or Dako it is not 
helping.  Dako has been great with sending in technical experts repeatedly and 
we cannot get this corrected.

Thanks,
Pam

--
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[Histonet] HHV-8 RTU

[Delray Beach Pathology Kari Simeone via Histonet]

My vendor (Cell Marque/Sigma Aldrich) has a YEAR backorder on this IHC RTU. 
Does anyone have a vendor they purchase from that I can research? Thanks so 
much.



Kari M Simeone

Histology/Immunohistochemistry Specialist Supervisor

Alternate Laboratory Supervisor









The information contained in this message and any attachments is intended only
for the use of the individual or entity to which it is addressed, and may 
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information that is privileged, confidential and exempt from disclosure under
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Re: [Histonet] Fwd:

[Rene J Buesa via Histonet]

As I see it, the best solution is "1"Even more: if the piece of tissue is large 
enough,→cut 1 section and stain → select at least 2 (+) areas→ divide the block 
into 2 blocks each containing one of those 2 areas and by doing so you would 
have duplicated the number of possible (+) sections.René 

On Monday, May 2, 2016 3:09 PM, Linda Margraf via Histonet 
 wrote:
 

 

> From: Cindy Bulmer 
> Date: May 2, 2016 at 12:15:22 PM CDT
> To: histonet-ow...@lists.utsouthwestern.edu
> 
> Hello Histoland,
>  
> I need some advice, I have a PT block that is positive with Spirochetes.
> What would be the best way to use this block as a positive control?
>  
> 1)  Cut (serial sections, stain the last slide for bugs) and oven time (60) 
> for 1 hr.
>      then put slides in refrigerator for future use.
> 2)  Cut (serial sections, stain the last slide for bugs) NO oven time  and 
> put slides
>      directly in refrigerator for future use.
> 3)  Cut "fresh" every time they order the Ab.
>  
> Thank you,
> Cindy
> Cynthia Bulmer HT(ASCP),QIHC
> IHC Supervisor, CTPL
> Waco, TX
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