[Histonet] Tissue controls error and appology

[Rachel M Gonzalez via Histonet]

Hi Everyone,

I want to send a sincere apology to the histonet group. I realized now that
I was wrong to send out this survey even if it was with the good intention
 to help my friend. I am sorry, I honestly did not realize the implication
and how unfair this was.

In the past, you have helped me to learn to cut tissue suggested great
antibodies, and given great advice on some practical applications. I feel
that this is an IHC community that I can turn to for help but realized now
although I was trying to help a friend in this format I was wrong.

I will not make out that mistake again. I hope you will accept my apology.

Sincerely,

Rachel
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Re: [Histonet] Poor Staining

[Debra Siena via Histonet]

Hi Charles 

Getting three shades of pink depends a kit on your dehydration steps after 
eosin.  Eosin is more soluble in water than alcohol so you have to watch how 
much water is being absorbed from the atmosphere and change/rotate alcohols 
when you see the last absolute turning pink, also if you have diluted alcohol 
after eosin you may wish to cut down or remove. I don't use a diluted alcohol 
at all just straight absolute alcohol. I recommend 3-4 changes of absolute 
alcohol for 1 minute each. 

For nuclear detail that could be caused by over processing and removing the 
bound water, If this is an occasional occurrence but if happening in all 
tissues large and small, It may also be that your hematoxylin and acid rinse 
need some type of adjustment. 

If I can help you offline please let me know.  Thanks

Debbie Siena, HT(ASCP)QIHC



Sent from my iPhone

> On Jun 16, 2016, at 1:20 PM, Charles Riley via Histonet 
>  wrote:
> 
> Can over processing small biopsies lead to poor chromatin staining? Also
> can this cause an issue with the eosin staining ( for example only getting
> to shades of pink instead of three)?
> 
> Please give me any feedback you can
> 
> -- 
> 
> Charles Riley HT(ASCP)CM
> 
> Histopathology Coordinator/ Mohs
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Re: [Histonet] Poor Staining

[Jennifer MacDonald via Histonet]

Heat can cause poor chromatin staining.  Is the two shades of eosin only 
on the biopsies, or all tissues? 



From:   Charles Riley via Histonet 
To: histonet@lists.utsouthwestern.edu
Date:   06/16/2016 11:19 AM
Subject:[Histonet] Poor Staining



Can over processing small biopsies lead to poor chromatin staining? Also
can this cause an issue with the eosin staining ( for example only getting
to shades of pink instead of three)?

Please give me any feedback you can

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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[Histonet] warthin-starry

[Jeff Halstead via Histonet]

Hi all thanx for the tips-I am not really allowed to use acid washes-don't have 
access to a reliable microwave-the stain is done by hand  with me assimilating 
the different reagents-or a mastertech kit. The idea of the bleach causing 
problems is interesting-before I was told not to use the strong acid washes-now 
that I ponder---I do have a bit of the old solution on hand might try one run 
with-nobody please mention this-with glassware washed the old fashioned way. 
Other than the bleach-am at loss as to cause of the precipitate. By the by  do 
not use any type of gelatin or adhesive in the water bath.
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[Histonet] Poor Staining

[Charles Riley via Histonet]

Can over processing small biopsies lead to poor chromatin staining? Also
can this cause an issue with the eosin staining ( for example only getting
to shades of pink instead of three)?

Please give me any feedback you can

-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] warthin-starry stain

[Jennifer MacDonald via Histonet]

are you doing a microwave method or traditional?



From:   Jeff Halstead via Histonet 
To: "histonet@lists.utsouthwestern.edu" 

Date:   06/16/2016 10:17 AM
Subject:[Histonet] warthin-starry stain



Hello histonet---I am a tech in western Oregon, and I have been 
experiencing strange problems with the afore mentioned stain. When the 
stain is run a very strange grey precipitate deposits upon the slides. The 
deposition is not uniform across the slides and varies from slide to slide 
within the run. I use bleach washed slides and all glassware is also 
bleach washed. The slides I am using are non charged with only frosted 
top. The last two runs I used reagents from just opened bottles. No meatal 
contacts the slides or the glassware used to assimilate the reagents. As I 
said---what gives. To say I am perplexed is understating my mood. I have 
even tried washing the slides in the hot water bath to no improvement. My 
path has a list of cases to run and is a bit anxious. Please help. Anyone 
with any ideas ? one step I did not mention-I bleach wash the slides-wash 
in di water-soak in 100% ethanol-then air dry and cover. Also thought I 
might mention I de-wax the pookers from 30 minut
 es to 45 minutes. Thanx for the time   jeff
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[Histonet] warthin-starry stain

[Jeff Halstead via Histonet]

Hello histonet---I am a tech in western Oregon, and I have been experiencing 
strange problems with the afore mentioned stain. When the stain is run a very 
strange grey precipitate deposits upon the slides. The deposition is not 
uniform across the slides and varies from slide to slide within the run. I use 
bleach washed slides and all glassware is also bleach washed. The slides I am 
using are non charged with only frosted top. The last two runs I used reagents 
from just opened bottles. No meatal contacts the slides or the glassware used 
to assimilate the reagents. As I said---what gives. To say I am perplexed is 
understating my mood. I have even tried washing the slides in the hot water 
bath to no improvement. My path has a list of cases to run and is a bit 
anxious. Please help. Anyone with any ideas ? one step I did not mention-I 
bleach wash the slides-wash in di water-soak in 100% ethanol-then air dry and 
cover. Also thought I might mention I de-wax the pookers from 30 minutes to 45
  minutes. Thanx for the time   jeff
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[Histonet] Histology Medical Waste

[Cassie P. Davis via Histonet]

In May,  Curt sent this out to Histoland:



"Message: 2
Date: Wed, 11 May 2016 20:18:52 +
From: Curt 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] embedding and microtomy "medical waste"
Message-ID:

<9C8F910F72893643B3C3793C3D67132B67CBAFD6@PATHOLOGYSERVER.pathologyarts.local>

Content-Type: text/plain; charset="us-ascii"

So here's a good one for you all... we had the county health department come 
through the lab and ding us on medical waste... specifically the plastic 
disposable lids at embedding, the lens paper used for wrapping specimens such 
as ECC and EMB. Then they got us on the Kim Wipes used to clean the water bath 
at microtomy... those papers have human tissue on them so they need to be 
treated as medical waste... NOW we have to have red cans next to all microtomes 
and embedding stations. The obvious issue outside of cost and logic is that 
these medical waste cans all seem to have self closing lids which really 
interferes with the rhythm and pace of work when one needs to reach over with a 
food to open the lid after every block is embedded and when they water bath is 
cleaned after every block...

Simple question(s): 1)does anyone else have to do such things to contain the 
waste, 2) does anyone know of a source for medical waste cans that do not have 
these frustrating self closing lids... if we could simple remove the lid and 
replace it when done then we could deal with it, the cost is one thing but 
slowing down work flow is a problem.

And just for a little more humor, they actually wanted me to contain and 
dispose of the water runoff from our two automated slide stainers, we run about 
2200 slides a night... that would be many gallons of waste water every night 
and would not be within the budget We in turn ran a fish kill test which 
demonstrated that the water runoff which contain little Hematoxylin, bluing and 
clarifier do not pose any significant threat to the environment, not even in 
California

Bottom line to all this, I need some red trash cans with removable lids, if 
they're still out there somewhere Anywhere.


Thanks for your input,

Curt

Ps, I didn't proff read thie smail... if something is not spelt correctly, 
don't hold it against me"



 I forwarded this to my supervisor, hoping to be proactive. She forwarded it to 
the person who is in charge of safety at our facitlity. He would like to know 
which states require the block and slide to be put in biohazard containers 
before we invest in the containers. Histofolks, please help another Histonetter 
out and let me know where you are and if you are required to put your shavings, 
block and or slides in biohazard medical waste.

Thanks!


Cassandra Davis
Histology Technician
Anatomical Pathology Laboratory
Saint Francis Healthcare
701 N. Clayton Street
Wilmington,DE 19805
Office:  302-575-8095
Email:  cda...@che-east.org
www.saintfrancishealthcare.org

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Re: [Histonet] Tissue Controls (Deceptive)

[Rachel M Gonzalez via Histonet]

Hi

I am really sorry to everyone.
You have always been so helpful to me I just wanted to help a colleague.
Sorry, I am a scientist and did not realize the implication as unfair.
I will not do that again.
Rachel

On Wed, Jun 15, 2016 at 3:52 PM, Jay Lundgren via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Get out the pitchforks and torches!
>
> On Wed, Jun 15, 2016 at 11:26 AM, Sally Price via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> > Not cool Rachel.  I took the survey assuming that it was intended and
> > might help a colleage, but it's nothing but a marketing ploy, which are
> > strongly discouraged on this forum.
> >
> >  Original message 
> > From histonet-requ...@lists.utsouthwestern.edu
> > Date: 06/15/2016  1:00 PM  (GMT-05:00)
> > To histonet@lists.utsouthwestern.edu
> > Subject Histonet Digest, Vol 151, Issue 14
> >
> > -
> >
> > Message: 1
> > Date: Tue, 14 Jun 2016 14:09:16 -0400
> > From: Rachel M Gonzalez 
> > To: "histonet@lists.utsouthwestern.edu"
> > 
> > Subject: [Histonet] Tissue controls
> > Message-ID:
> > 
> > Content-Type: text/plain; charset=UTF-8
> >
> > Hi Everyone,
> >
> >
> > I am trying to help out a coworker with a survey on types of tissue and
> > controls needed for both clinical and research labs. She has a short
> > deadline by next Monday.
> >
> > She if in charge of the OriGene Technologies tissue division.  If you
> could
> > take a few minutes to fill in the survey for a really nice coworker
> (Julie)
> > that would be great.
> >
> > https://www.surveymonkey.com/r/QMGWKHZ
> >
> >
> > Thanks for any help.
> >
> > Rachel
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> >
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