Re: [Histonet] Suggestion for Dual staining Kit/
Hi Nasir, I use Biocare reagents (DAB, Warp Red and Ferengi Blue) for my multicolour stuff. Even though the Vector blue fluoresces you should still see what you need to see in brightfield. If you're worried about losing your signal through solvents you could probably just airdry before coverslipping, that's what I do (using Ecomount). The xyelene is only a problem after you put the chromogen on, not before. Looks like you have 2 rabbit and 1 rat Ab for your Abs, it may be possible to do a triple stain with that with a rat and rabbit in the first round and the other rabbit in the 2nd or you should be able to combine at least a rat and rabbit in a double simultaneous or 2 rabbits in a double sequential stain depending on your Abs and how robust they are. If you don't have access to spectral imaging though it may be safer going with doubles so you can pull everything apart by eye. Is that CD8 Ab behaving ok for you? Looks like it has a ton of background in the images. If you're having issues with it you may be able to combine your CD4 Ab with the CD3 SP7 clone to show the presumptive CD8s. I haven't worked with any of those Abs but I do do multicolour IHC in both human and mouse tissues so if you need help sorting out protocols let me know, of course I can only speak to Biocare reagents for this as that's what I usually use. Katy Milne Deeley Research Centre Message: 2 Date: Fri, 4 Nov 2016 15:36:54 + (UTC) From: Nasir Abbas To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] Suggestion for Dual staining Kit/reagent Message-ID: <735822620.281524.1478273814...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hi All Can some one suggest dual staining reagents for IHC-P (mouse liver tissue) for my primary antibodies: ab183685, GTX53126 and ab25377.I was suggested vectors labs products (following table) but I am not clear about Vector Blue reagent SK-5300 that whether it can be used in IHC-P, because it features shows it is fluorescent. Moreover it is sensitive to xylene and Histoclear may be used as a clearing reagent in this case, which makes me even more worried. Any suggestion and tips for my dual staining?Table: | Pri-Ab | Vector Lab Products- Suggested | | ab183685 | MP-7401-15 | | GTX53126 | SK-4105 | | ab25377 | MP-5404-15 | | ? | SK-5300 | ? Sincerely?Nasir Abbas ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] In need of a temporary histotech in San Diego
Good Afternoon, We are looking for an experienced histotech (HT/HTL certified) who can work as a temp full-time. We need this person to start the week of November 14, 2016. If this is you, please contact me. My work email is psicure...@ucsd.edu, my phone # is 619-543-2872. Sorry for the short notice, but stuff happens, you know what I mean? Happy Friday. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist/Interim Supervisor Histology, EM, IHC UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Transporting wet tissues
Helen, we use Coleman 50-qt wheeled coolers (with wheels and handles). The containers and buckets are parafilmed and put in plastic bags. The coolers have formalin absorbing pads at the bottom for any leaks. The coolers are liquid proof, easy to roll around. Occasionally a handle will break on one, but we keep spares at each site. We use these for couriering all our paperwork and specimens between 3 sites. We don't courier too many large specimens, but we do courier a lot of cassettes in baskets for processing from two sites to a central histo lab for processing. Same issue - containing the formalin in a container. https://www.amazon.com/Coleman-50-Quart-Wheeled-Cooler-Blue/dp/B000G33YT0/ref=sr_1_2?ie=UTF8&qid=1478280100&sr=8-2&keywords=50+qt+wheeled+cooler Tim Morken Pathology Site Manager, Parnassus Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: Howe, Helena via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, November 04, 2016 10:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Transporting wet tissues Does anyone have a system for transporting large specimens to Your lab. We are building an offsite lab for Anatomic Pathology and are looking for a safe way to transport the large specimens that are in buckets of formalin. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Her2 Reflex
Re: Her2 by IHC We reflex 1+ to FISH, too Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Transporting wet tissues
Does anyone have a system for transporting large specimens to Your lab. We are building an offsite lab for Anatomic Pathology and are looking for a safe way to transport the large specimens that are in buckets of formalin. _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ EMAIL CONFIDENTIALITY NOTICE This Email message, and any attachments, may contain confidential patient health information that is legally protected. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this message is strictly prohibited. If you have received this information in error, please notify the sender immediately by replying to this message and delete the message from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Suggestion for Dual staining Kit/reagent
Hi All Can some one suggest dual staining reagents for IHC-P (mouse liver tissue) for my primary antibodies: ab183685, GTX53126 and ab25377.I was suggested vectors labs products (following table) but I am not clear about Vector Blue reagent SK-5300 that whether it can be used in IHC-P, because it features shows it is fluorescent. Moreover it is sensitive to xylene and Histoclear may be used as a clearing reagent in this case, which makes me even more worried. Any suggestion and tips for my dual staining?Table: | Pri-Ab | Vector Lab Products- Suggested | | ab183685 | MP-7401-15 | | GTX53126 | SK-4105 | | ab25377 | MP-5404-15 | | | SK-5300 | Sincerely Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue Science Park Guangzhou, 510530, Guangdong P.R.ChinaCell: +86-1312 8296 614 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
