[Histonet] PRN Histo position

[Steven Crochiere via Histonet]

Currently seeking an experienced HT for dermpath/MOHS laboratory in
Springfield, MA to cover for absences and possibility to become FT  in
future.

Contact:

Terry Molnar

New England Dermatology

3455 Main St

Springfield, MA 01107

tmol...@nedlc.com  

(413)733-9600 x319

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[Histonet] BD PROBETEC

[Baldwin, Kathy via Histonet]

Hi Histonetters!!
   We have a probetec unit that our histology department is willing 
to sell.  Below is conformation of the manufacturer date.  It was taken out of 
service this past fall and was in full working order.  It does have a water 
bath and heat block (the heat block is having issues maintaining a temp).  The 
model is BD Probetec ET, serial number: PT 2782.  I will get ahold of our 
materials management department if this is something any of you are interested 
in.



Thanks
S. Kathy Baldwin
Histology/Cytology Supervisor
Memorial Hospital and Health Care Center
800 West 9th St.
Jasper, Indiana  47546
Office 812-996-0210
Fax 812-996-0232





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[Histonet] Selling a Ventana Discovery XT

[Allan Wang via Histonet]

Hello list,

The last message reminded me:

My lab is upgrading from the Discovery XT to the Ultra so we're selling the
XT. We're offering it for a great price compared to new. It'll come with
the computer, slide label printer, accessories, and all the reagents -
everything needed to get started. It's only been used for 7 months and is
still under the original first year service contract (doubt it will
transfer though)

If anyone is interested, I'd be happy to discuss the pros and cons of the
system. It is located in Rockville, MD.

Here is an example of a double stain:
http://www.biomax.us/images/ER-Brown_HER2-Purple_core.jpg

Allan Wang
Lab Manager
US Biomax
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Re: [Histonet] need automation system for fluorescent Multiplex IHC

[Maria Mejia via Histonet]

I forgot to mention in my email below our sections will be paraffin for 
Multiplex
fluorescent.

Thank you
> On Feb 10, 2017, at 10:43 AM, Maria Mejia  wrote:
> 
> Dear All,
> 
> I work in a research lab where the focus is on early stages of Alzheimer’s 
> Disease.
> We are seriously considering using an automated staining system for 
> fluorescent Multiplex
> IHC, for the simultaneous detection of multiple (2-6 or more) proteins of 
> interest in 
> FFPE brain sections.
> 
> However, our lab has never used an automated staining system before, so we 
> lack
> the abilities and experience to make the correct choice for our lab’s needs.
> 
> Cost; the price of a USED system, since we are a research lab since a new 
> system is more
> expensive, user friendly & size of system are of course major considerations 
> as well 
> as the consumables that go along with the system e.g. reagents, containers, & 
> anything else
> to consider as well.
> 
> Please, I’m reaching out to anyone (system users & vendors) who can help our 
> lab make a smart choice.
> I would be most grateful for any recommendations or suggestions. I very much 
> look forward to hearing
> from you.
> 
> Best
> Maria Mejia
> Lead Histologist
> UCSF 
> Memory & Aging Center
> 
> 

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[Histonet] New lab

[Emily Horst via Histonet]

Hi everyone,

I am in the process of setting up a new lab. We will process FNA specimens
as cell blocks and then treat them as regular histology specimens.

I am curious as to finding a proper VIP schedule for processing these
specimens. Any suggestions??

Thanks in advance,

Emily Horst

-- 
Emily Horst, HT(ASCP)
Office Manager
Interventional Pathology of Ohio, LLC
Kettering OH 45429
937-297-0028
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[Histonet] need automation system for fluorescent Multiplex IHC

[Maria Mejia via Histonet]

Dear All,

I work in a research lab where the focus is on early stages of Alzheimer’s 
Disease.
We are seriously considering using an automated staining system for fluorescent 
Multiplex
IHC, for the simultaneous detection of multiple (2-6 or more) proteins of 
interest in 
FFPE brain sections.

However, our lab has never used an automated staining system before, so we lack
the abilities and experience to make the correct choice for our lab’s needs.

Cost; the price of a USED system, since we are a research lab since a new 
system is more
expensive, user friendly & size of system are of course major considerations as 
well 
as the consumables that go along with the system e.g. reagents, containers, & 
anything else
to consider as well.

Please, I’m reaching out to anyone (system users & vendors) who can help our 
lab make a smart choice.
I would be most grateful for any recommendations or suggestions. I very much 
look forward to hearing
from you.

Best
Maria Mejia
Lead Histologist
UCSF 
Memory & Aging Center


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Re: [Histonet] Immunohistochemistry ghosting

[Duddey, Aimee via Histonet]

When we saw this happening it was slide related, especially with it happening 
across all platforms.  We use Cardinal Healthcare SuperFrost Plus and haven't 
had an issue.  Every time we have tried another brand we have seen this happen 
to some degree. 

Aimee M. Duddey, MLT(ASCP)
Assistant Director of Laboratory - Pathology
FH Moore Regional Hospital
910-715-5286 office
910715-1944 fax


-Original Message-
From: Robyn L. Vazquez via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, February 09, 2017 7:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Immunohistochemistry ghosting

Hello,
We have been experiencing problems with ghosting (inconsistent and/or no 
staining) mainly affecting our control area which is located at the bottom of 
the slide.  Random antibodies markers are being affected. We were using Fisher 
Scientific then TruBond slides. We are using Ventana Ultras X4 and XT in which 
it is occurring on all machines. Ventana has come in to troubleshoot, in which 
we have not reached a solution.

Any suggestions would be greatly appreciated

Robyn L. Vazquez
Kaiser Permanente
Portland, Oregon
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