[Histonet] H&E on frozen sections

[Ana Maluenda via Histonet]

Hi everyone,

Just wondering if anyone out there had already had issues with commercial 
Haematoxylin and Eosin. I started working in a lab that recently purchased 
Sigma Mayer's Haematoxylin (MHS-16) and 1% Alcoholic Eosin (Fronine - II016). 
We work with fresh frozen sections of mouse tissue. Since then, H&E protocol 
haven't worked well. I tried different protocols, following the product sheet 
as well as following other researcher's protocols and none worked. Both 
Haematoxylin and Eosin are very faint, with Eosin been uptake very 
inconsistently even on the same section (some of the areas are not even stained 
at all). I also used sections from different projects, with different people 
performing tissue harvest, OCT embedding and sectioning (so it looks more a 
problem with the staining protocol rather than the specimens) and tried 
increasing the staining times.

Any ideas would be much appreciated. Also, what are people using as commercial 
staining Haem and Eosin (brand and catalogue number)?

Thanks very much!

Kind regards,

Ana

Ana Maluenda
Research Assistant
Atherothrombosis and Vascular Biology Laboratory

Baker Heart and Diabetes Institute
75 Commercial Road, Melbourne VIC 3004
P (03) 8532 1359 E ana.malue...@baker.edu.au 
W www.baker.edu.au

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[Histonet] Gold Chloride Source

[ian bernard via Histonet]

Histonetters, looking for Gold Chloride purchase source. Current distributor
has the product on back order- Sigma Aldrich. Need another source since we
are running low.  Please provide catalog number as well.

 

IB

 

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[Histonet] Cryostat and Parts

[Travis Troyer via Histonet]

We are in the process of moving and we have some equipment and supplies
that we are not taking with us.We have 2 Microm HM 505E cryostats and
all of the internal components.One of the cryostats doesn't cool down
and the other one doesn't advance properly but is otherwise in working
order.If anyone is interested in the cryostats or the any of the parts
please let me know.We also have 12 bottles of Polarstat Plus cryostat
mounting media (exp 6/18).If anyone is interested please let me know.

Travis Troyer

Advanced Dermatology and Skin Cancer Center
1133 College Ave., Suite E230
Manhattan, KS 66502
Tel: 785-537-4990 <(785)%20537-4990>
Fax: 785-537-1938 <(785)%20537-1938>
d...@ksderm.com
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Re: [Histonet] . Refurbished Leica RM2135

[Mayer,Toysha N via Histonet]

Sandra,

On some holders you can easily convert back and forth from low to high.  If 
your service person does not know anything about that, I would ask Leica.  
Specifically ask for a tech rep with the most microtome experience (in years).  
That person should be able to tell you what part you need.  They may even have 
an old one lying around for you.
Also check with some of the more established labs in your area, they may have a 
blade holder that you can get for parts.

Toysha Mayer




Message: 2
Date: Tue, 02 May 2017 14:10:05 +
From: Sandra Cheasty 
To: "Histonet (histonet@lists.utsouthwestern.edu)"

Subject: [Histonet] Refurbished Leica RM2135
Message-ID:



Content-Type: text/plain; CHARSET=US-ASCII

Hi everyone,
I have a newly refurbished Leica RM2135 at my disposal, but the 
knife holder is for high profile blades. 2 questions.
1-Is it that different using high-profile blades vs. low. (I have used 
low-profile blades forever.) 2-There is a slot on the bottom of the blade 
holder, and I seem to remember that it can be converted to a low-profile holder 
by sliding an narrow metal strip into that slot. I know I've done this before, 
but it was long ago, and the used equipment guy says there's no such thing.

Thank you!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine



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Re: [Histonet] Refurbished Leica RM2135

[Пешков Максим via Histonet]

From sugh expensive blades can be done more very good sections (at 3 microns) 
from any difficult type tissue.
These investments will pay off very quickly.
Maxim Peshkov
Russia,
Taganrog.


>Вторник,  2 мая 2017, 21:16 +03:00 от "Howery, Jeff L" 
>:
>
>But, high profile knives are more expensive. This is due to the increased 
>amount of Stainless steel needed to make them. 
>
>-Original Message-
>From: Пешков Максим via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
>Sent: Tuesday, May 02, 2017 11:12 AM
>To: Sandra Cheasty
>Cc:  histonet@lists.utsouthwestern.edu
>Subject: Re: [Histonet] Refurbished Leica RM2135
>
>
>Sandra, high profile blades was better for me, because they are harder and 
>more durable than low profile of any manufacturer. Also I recommend for you to 
>try Surgipath DB80HS. They must better than 818. I regret that not having 
>bought a holder for a high profile blades.
>Leica makes the blade holders only for one type blades (low or high).
>Microm's blade holder made for both type blades.
>--
>Sincerlely,
>Maxim Pehkov
>Russia,
>Taganrog.
>
>
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Re: [Histonet] Refurbished Leica RM2135

[Пешков Максим via Histonet]

Sandra, high profile blades was better for me, because they are harder and more 
durable than low profile of any manufacturer. Also I recommend for you to try 
Surgipath DB80HS. They must better than 818. I regret that not having bought a 
holder for a high profile blades.
Leica makes the blade holders only for one type blades (low or high).
Microm's blade holder made for both type blades.
--
Sincerlely,
Maxim Pehkov
Russia,
Taganrog.


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[Histonet] AFB Control Blocks

[Clarence Owens via Histonet]

Good Morning All,

I am in search for 2 blocks of AFB positive lung blocks. Does anyone have 2
blocks they could spare. I am willing to trade a control block for another
control block. I thank you for your help in advance.

Regards,

- Clarence Owens
​, HT (ASCP) QIHC​
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[Histonet] Refurbished Leica RM2135

[Sandra Cheasty via Histonet]

Hi everyone,
I have a newly refurbished Leica RM2135 at my disposal, but the 
knife holder is for high profile blades. 2 questions.
1-Is it that different using high-profile blades vs. low. (I have used 
low-profile blades forever.)
2-There is a slot on the bottom of the blade holder, and I seem to remember 
that it can be converted to a low-profile holder by sliding an narrow metal 
strip into that slot. I know I've done this before, but it was long ago, and 
the used equipment guy says there's no such thing.

Thank you!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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