[Histonet] PIN4

[Renee Fisher via Histonet]

Need help the PIN4 has suddenly stop working we use P63+CK5+CK14 from Bio Care 
Medical and P504S from Dako.  We double stain on the Leica Bond with the 
Polymer refine Red and Polymer Refine detection. This stain has worked 
beautifully in the past. Any suggestions?



Thank you

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[Histonet] new dako omnis machine vs current dako autostainer link 48

[cafechague via Histonet]

hi all!I'm validating our dako omnis. it is a brand new platform.  and I'm 
aware that ten positive and negative controls for antibody validations are 
needed for cap regulations.we are currently using the older dako stainerlink48 
with all our antibodies already validated, but plan on moving it to a different 
location. am i to perform the same protocol for both new and old stainers? or 
can i just validate 5 positive and negative for the autostainerlink48, instead 
of having to validate 10.any feedback is appreciated!-cae


Sent from my Verizon, Samsung Galaxy smartphone
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[Histonet] biobond experience?

[Travers, Susan via Histonet]

Hi all,

Just wondering if anyone has had any experience using biobond as an adhesive 
for coating slides.  We have used a chrome-alum/gelatin coating for years with 
formalin-fixed, frozen brain sections and it works pretty well. However, with 
certain stains (e.g., black-gold) we continue to have periodic problems with 
sections loosening from the slide.  Wondering if Biobond might be an 
improvement?

Thanks for any insights

ST

Susan Travers
Professor, Biosciences
Division of Biosciences, College of Dentistry
The Ohio State University
305 West 12th Avenue
Columbus, Ohio  43210
traver...@osu.edu
(614)361-0800 (cell)
(614)292-6366 (work)

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Re: [Histonet] Adenovirus antibody?

[Jan Shivers via Histonet]

Hello Paula,

This isn't an answer to your question, because I do use the Millipore
antibody (MAB805) and it works quite well on a Dako platform or done
manually.  It requires enzyme digestion antigen retrieval.  I use it at a
1:400 dilution (45 min) with a polymer detection system (45 min).

I'm not sure why it works on one platform but not another.

Best of luck,


Jan Shivers
Senior Scientist
IHC/Histology Section Manager
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive...@umn.edu

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On Thu, Aug 3, 2017 at 8:47 PM, Tony Henwood (SCHN) via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Paula,
>
> We use Adenovirus antibody from BioSB (Diagnostic Technology)
> Cat Number  BSB 5040
> Clone   20/11 and 2/6
> Isotype IgG1/K
> Dilution1:200 with citrate retrieval on the Bond 3
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Principal Scientist, the Children's Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -Original Message-
> From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Friday, 4 August 2017 8:50 AM
> To: HistoNet
> Subject: [Histonet] Adenovirus antibody?
>
> Hello Listers,
>
> I am listing to starboard on this one.  We are using the Ventana Ultra
> staining platform and have had a bit of trouble with our Adenovirus
> stains.  We have used Abcam and Millipore to no avail.
>
> Do you have suggestions about another vendor to try?  We are a clinical
> lab so we only stain human tissue.  IVD is preferred but ASR and RUO are OK
> too.
>
> Any suggestions are gratefully accepted.
>
> Thank you in advance.
>
> Sincerely,
>
> Paula Sicurello, HTL (ASCP)CM
>
> Histotechnology Specialist
>
> UC San Diego Health
>
> 200 Arbor Drive
>
> San Diego, CA 92103
>
> (P): 619-543-2872 <#>
>
>
>
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[Histonet] Happy Friday--Lab related blog post

[Lester Raff MD via Histonet]

Have a good weekend-and I hope your lab works like ours!

http://www.chicagonow.com/downsize-maybe/2017/08/we-are-great-because-you-wont-stop/

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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