Re: [Histonet] The Use of Plants in Histology Laboratories (Mickley, Beth)

2017-11-14 Thread Mayer,Toysha N via Histonet 
Beth,

We sure could have used the actual article in a lab I know of.  The person with 
the highest authority, removed them from a lab, and did not want to listen to 
what the supervisor had to say.  Without the actual article, nothing could 
change her mind.
It is common to have spider plants, and ivy in labs to help with the air 
quality. 
Now the EHS departments need to know about it as well.

Toysha

Message: 1
Date: Mon, 13 Nov 2017 20:24:31 +
From: "Mickley, Beth" 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] The Use of Plants in Histology Laboratories
Message-ID: <1510604671172.29...@urmc.rochester.edu>
Content-Type: text/plain; charset="Windows-1252"

I found this great article about plants used in laboratories:

Plants That Can Clean Up Your Indoor Air

Plants clean indoor air in two ways?by absorbing contaminants through pores on 
the leaves, and by metabolizing contaminants through organisms living in the 
soil. In fact, plants are so effective that some stores, like Lowe?s and Home 
Depot, are starting to label the most effective ones with tags. 

Though it seems most plants will benefit indoor air, the following are those 
that have been shown in scientific studies and shown to work. These plants can 
also help maintain humidity levels and remove mold spores and bacteria from the 
air. 
1.Spider Plant: formaldehyde, xylene and toluene.
2.Golden Pothos: benzene, formaldehyde, trichloroethylene, xylene and toluene.
3.Snake Plant (Mother-in-Law?s Tongue): benzene, formaldehyde, 
trichloroethylene, xylene and toluene.
4.Bamboo Palm or Reed Palm: formaldehyde, xylene, and toluene.
5.Chinese Evergreen: benzene, formaldehyde.
6.Peace Lily: benzene, formaldehyde, trichloroethylene, xylene, toluene, and 
ammonia.
7.English Ivy: mold and mildew, formaldehyde, benzene, xylene, and toluene.
8.Gerbera Daisies: benzene, formaldehyde, trichloroethylene.
9.Red-Edged Dracaena (Dracaena Marginata): benzene, formaldehyde, 
trichloroethylene, xylene, and toluene. 
10.Warneck Dracaena: benzene, trichloroethylene, xylene, and toluene.
11.Weeping Fig: formaldehyde, xylene, and toluene.
12.Chrysanthemum: formaldehyde, benzene, trichloroethylene, xylene, toluene, 
and ammonia.
13.Boston fern: formaldehyde, xylene and toluene.
14.Philodendron: formaldehyde.


Beth Geer, HT
Mohs Surgery
University Dermatology Associates
Rochester, NY



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[Histonet] 2018 FSH Annual Meeting

2017-11-14 Thread John Shelley via Histonet 
Hello Histonetters!

The FSH Annual Meeting will be in Tampa, FL on May 17-20, 2018 at the 
Renaissance Tampa International Plaza Hotel.

We are looking for ideas for classes that you will be interested in attending.

We are also looking for speakers. Send abstracts to email below.

We want to hear from you. Contact us at fshgrouppresidentgmail.com

See you in Tampa.

Kind Regards!

John J Shelley
fshgrouppresid...@gmail.com

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Re: [Histonet] Mounting media

2017-11-14 Thread Terri Braud via Histonet 
Although we've used several brands with good success, our most consistent 
performer has been the Sakura Tissue Tek Glas Mounting Media #6419. When 
coverslipping, either automated or manual, the secret to avoiding air bubble 
during storage is to insure that the correct amount of media is dispensed onto 
the slide.  If the amount is insufficient, the slide will still coverslip to be 
read, but as time passes and the xylene dries out, there will be air left under 
the coverglass which will allow the stain to degrade. When coverslipping by 
hand, we go by the rule of 3 drops of media from a plastic disposable pipette 
for a 24x50 No.1 coverglass.  When techs "guesstimate" is when problems occur.  
Best of luck, I hope this helps

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal



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Re: [Histonet] mounting medium

2017-11-14 Thread Mary Lou Norman via Histonet 
Refrax from Anatech

-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, November 13, 2017 5:47 PM
To: warda hassan
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium

Hi,

This information would be very useful for me and probably others as well, since 
I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help 
> preservation of staining properties without creating fading of stains 
> and bubbles on long run.
> Thanks alot
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Re: [Histonet] mounting medium

2017-11-14 Thread Diane Satterfield via Histonet 
We use Sub-X Mounting Medium from Leica.  This is what the pathologist wants us 
to use.

-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, November 13, 2017 5:47 PM
To: warda hassan
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium

Hi,

This information would be very useful for me and probably others as well,
since I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help preservation
> of staining properties without creating fading of stains and bubbles on
> long run.
> Thanks alot
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=imBPVzF25OnBgGmVOlcsiEgHoG1i6YHLR0Sj_gZ4adc&r=eLj2B87Mkll0NUAmalTD_1fRsqzhM-r-54bMn0ykFbs&m=qAeXtP7MVtiB5tSOrrA22EAPWyiHIuG7ijlXdENpf68&s=-1VDk505lt6WIaFntwlARL_RqLOgFH02KVXBwp7kovE&e=
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[Histonet] HSV I/II Control

2017-11-14 Thread McCabe, Sara via Histonet 
Awhile ago I had posted looking for anyone that had any in house HSV controls.  
A gentleman had reached out to me; however, I never did receive the controls 
that he said he was sending me.

I am trying to reach him.



I am also looking for anyone else that may also have HSV controls. We are in 
need.  I would rather not purchase commercial control slides.



Thank you in advance for your help!
Sara J. McCabe, HT(ASCP)CM
Histology Supervisor
Penn Highlands DuBois
100 Hospital Avenue
DuBois, PA 15801
814-375-7514 Telephone-Office
814-375-3264 Telephone-Histology  Lab
814-375-3784 Fax
sjmcc...@phhealthcare.org
www.phhealthcare.org

[PHH ESig Logo 150dpi-01]
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[Histonet] R: EDTA decalcification tissue issues

2017-11-14 Thread MANTERO Stefano RIC via Histonet 
Good Morning Kelly,

I work in a research institute that works with murine samples; In our 
laboratory we have adopted two descaling protocols: one with ion exchange 
resins/acid solution while the other uses EDTA.
The EDTA descaling system is slower but keeps the nucleic acids much better.
We use an 14% solution of tetrasodium EDTA in water buffered to pH 7.4-7.6 with 
acetic acid, the solution is left for several days (from 5 to 10 depending on 
the density and size of the bone) with daily solution changes.

Have a good work

Stefano

Dott. Stefano Mantero

Human Genome Laboratory  CNR-IRGB
c/o Humanitas Research Hospital

Via Rita Levi Montalcini (Ex Via Dainese)
20090 Pieve Emanuele (MI)

Ph. +39 02 8224 5164 (desk)
Ph. +39 02 8224 5177 (lab)
Fax +39 02 8224 5191



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-Messaggio originale-
Da: Pairan, Kelly via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Inviato: venerdì 10 novembre 2017 12:39
A: histonet@lists.utsouthwestern.edu
Oggetto: [Histonet] EDTA decalcification tissue issues

Good Morning,

Recently, our lab has been working on validating an EDTA method of 
decalcification.  When we ran the IHC's on the decalcified bone block, the 
majority of the tissue lifted off the slide.  We use the Leica Bonds for our 
IHC staining.  Does anyone else have a hard time getting EDTA decalcified 
tissue to stay on positively charged slides during IHC runs?  Do you have a 
trick you use?  We really did not have this big of a problem when we were 
decalcifying with Formical or Nitrical (big bones only).


Thanks for your help,

?Kelly Pairan, HT(ASCP), QIHC(ASCP)
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