[Histonet] Proscia
Has anyone used Proscia for digital imaging? Comments? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 175, Issue 15
To unsubscribe, please follow this link http://lists.utsouthwestern.edu/mailman/listinfo/histonet Victor Sent from Xfinity Connect Mobile App -- Original Message -- From: Nava, Josefa via Histonet To: histonet@lists.utsouthwestern.edu Sent: June 18, 2018 at 4:25 AM Subject: Re: [Histonet] Histonet Digest, Vol 175, Issue 15 unsubscribe -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Saturday, June 16, 2018 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 175, Issue 15 [EXTERNAL] Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest. .." Today's Topics: 1. Staining Protocol for Chromosome Counting in Plants (Bharti Parihar) -- Message: 1 Date: Fri, 15 Jun 2018 15:09:00 -0700 From: Bharti Parihar To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staining Protocol for Chromosome Counting in Plants Message-ID: Content-Type: text/plain; charset="UTF-8" Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be theright stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm us ed to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it! -- Bharti Parihar, HT (ASCP)CM -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= -- End of Histonet Digest, Vol 175, Issue 15 * The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If yo u are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] benchmark special stain free sol
I have 1 bottle of LCS and 1.5 bottle of depar solution for the benchmark special stain platforms, send me a fedex or ups number if you want them. Curt CONFIDENTIALITY NOTE: The information transmitted, including attachments, is intended only for the person(s) or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this in error, please contact the sender and destroy any copies of this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Anti-RET
Does anyone out there run the Anti-RET IHC from ABCAM (ab134100) on the Leica bond platform. I am about to work it up on out system and want a good starting point rather than testing every option and wasting time and money. 1. Do you use any enzyme treatment 2. Do you use ER1 or ER2 and for what times? 3. What do you find to be a good dilution ratio? Any assistance will be greatly appreciated -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 175, Issue 15
unsubscribe -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Saturday, June 16, 2018 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 175, Issue 15 [EXTERNAL] Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Staining Protocol for Chromosome Counting in Plants (Bharti Parihar) -- Message: 1 Date: Fri, 15 Jun 2018 15:09:00 -0700 From: Bharti Parihar To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staining Protocol for Chromosome Counting in Plants Message-ID: Content-Type: text/plain; charset="UTF-8" Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be the right stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm used to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it! -- Bharti Parihar, HT (ASCP)CM -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= -- End of Histonet Digest, Vol 175, Issue 15 * The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 175, Issue 15
unsubscribe -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Saturday, June 16, 2018 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 175, Issue 15 [EXTERNAL] Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Staining Protocol for Chromosome Counting in Plants (Bharti Parihar) -- Message: 1 Date: Fri, 15 Jun 2018 15:09:00 -0700 From: Bharti Parihar To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staining Protocol for Chromosome Counting in Plants Message-ID: Content-Type: text/plain; charset="UTF-8" Hi ya'll. If there are any individuals out there that have done karyotyping, I need to try a protocol to get a chromosome count on a desert plant. We have germinated seeds with long roots, so sample size shouldn't be an issue. Tried a Orcein stain, but the results are quite lacking and it may not be the right stain for this plant. Gonna do a bit of modification with the fixative to specimen ratio to see if that helps, but it's possible this just isn't the right stain. After all, the stain ordered is for Arabidopsis, which is not our research plant. This is my first time doing plant histo, I'm used to human tissue, so I really don't know which stain to try. Anyone with any help/advice to offer, I would immensely appreciate it! -- Bharti Parihar, HT (ASCP)CM -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=tAAbe3VWSXg4_eMSItYKuRlnp5vCb2OrQZQHnRXk_fc&r=h0EwiXOa8WlT7rjYHe7TZnXzwRXmblPlGcETNHHbbyE&m=RCrqLpf9kzw7THpxjY2pykyQ5Qb5lW6jfciAqUZ2Ho4&s=DvuYiGLJ75JPR6bFHU4b-c7vOdBOQgdz99K76SsRs_c&e= -- End of Histonet Digest, Vol 175, Issue 15 * The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
