Re: [Histonet] guinea pig IHC
following. I haven't done frozen stains on GP only FFPE...interested in what others have to say. Colleen On Tue, Jan 29, 2019 at 1:58 PM Jan Shivers via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Has anyone ever performed IHC on frozen sections of guinea pig tissue? I > am experiencing an enormous amount of bubbling when doing the peroxidase > blocking step, even though I'm only using a 0.3% concentration of H2O2. > And when I say 'enormous', I mean it's like continuous champagne bubbles > rising out of the tissue, even after 20 minutes in the H2O2 solution. > > I can't find anything in the literature that mentions guinea pigs having a > higher peroxidase content in their tissues. > > Thanks for any help that anyone can provide. > > Jan Shivers > Senior Scientist > IHC/Histology Section Manager > Pathology Teaching Program > Veterinary Diagnostic Laboratory > University of Minnesota > 1333 Gortner Ave. > St. Paul, MN 55108 > 612-624-7297 > shive...@umn.edu > > *Confidentiality Notice: This message, together with any attachments, is > intended only for the use of the individual or entity to which it is > addressed and may contain confidential or privileged information. If you > think you have received this message in error, please advise the sender and > then delete this message and any attachments immediately.* > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory B173 PWB 612-626-1930 *If submitting histology request please also forward to Lori Holm at ho...@umn.edu * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] guinea pig IHC
Has anyone ever performed IHC on frozen sections of guinea pig tissue? I am experiencing an enormous amount of bubbling when doing the peroxidase blocking step, even though I'm only using a 0.3% concentration of H2O2. And when I say 'enormous', I mean it's like continuous champagne bubbles rising out of the tissue, even after 20 minutes in the H2O2 solution. I can't find anything in the literature that mentions guinea pigs having a higher peroxidase content in their tissues. Thanks for any help that anyone can provide. Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cameras in the gross room
Hello Izak, The cameras at embedding show a view of the embedding hot plate and embedders lap. I would be surprised to meet a histo tech who has never experienced a piece of tissue popping out of a cassette when opening it up. The cameras are useful for identifying where the tissue might have jumped to. In addition, should there be any other type of error or floater tissue in a block, the cameras can be reviewed to see where the mystery tissue came from. When trouble shooting source of errors you can also check to see if the techs are following best practices, like cleaning tools after each use and cleaning the hot plates between blocks. Having cameras at gross and embedding allow you to determine at what point floaters could have been introduced in the process or reconcile any discrepancies between gross and embedding. Without the cameras, we could only make our best guess when it comes to troubleshooting. By reviewing camera footage it is easier to identify the real root cause of an issue so that you can improve your process and reduce future errors. In addition to histology, we also have cameras in our accessioning area where the specimens are initially received. I work for a company with multiple labs throughout Washington. We recently opened a new facility and it did not have cameras set up for the first six weeks or our operations. It was a great reminder of how helpful and important these cameras are. We would never go back to a camera free environment. Our cameras are IQeye cameras, and the camera footage is managed by OpenEye video surveillance software. I hope this is helpful information. Miranda Giorgi -Original Message- From: Izak Dimenstein via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, January 29, 2019 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cameras in the gross room Dear Miranda Giorgi: What is the purpose to have cameras, especially at the embedding station? What kind of cameras do you use? Thank you, Izak Dimenstein ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the InCyte Privacy Officer at priv...@incdx.com or call (509) 892-2700. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Can I Warm You Up With some Sizzlin' Hot Histo Opportunties?
Hello Histonetters, I hope you are having a great week. I wanted to send a quick note to tell you about the positions that I am working on and am most excited about. Why am I excited about these positions? Because each of these clients was asked if I had someone for you today would you be ready to interview and hire Every One of these clients said YES!!! Here is THE Top! Super Sizzlin Hot Spotlight Opportunity! Histotech Norfolk, VA Day shift 9K sign on bonus!! These are the Sizzling HOT Super Leadership Opportunities!! Territory Sales Manager Ohio Territory Sales Manager Texas AP Manager Milwaukee, WI These are the Sizzling HOT Super HT/HTL Opportunities!! Histotech Annapolis, MD Histotech Norfolk, VA Histotech Springfield, MA Histotech NYC, NY NY license req. Histotech Chattanooga, TN Histotech Atlanta, GA Histotech Milwaukee, WI All of these positions are full time and permanent!! My clients offer excellent compensation, benefits and in most cases either relocation or a sign-on bonus. Histonetters, if you think you or someone you know might be interested in any of these opportunities or would like to talk about a job search in another area, please contact me. If I place someone that You refer You will earn a referral fee. If you are interested in any of these opportunities CALL /TEXT MY CELL ASAP!!! At 407-353-5070. I can also be reached toll free at the office at 866-607-3542 or at rel...@earthlink.net to set up a time to talk at your convenience! Thank you so much for taking the time to read my email. I really appreciate it!! Thanks- Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] glass slides
That's interesting, we've been seeing warping with the Leica coverslips. I wonder if there's something in common in their manufacturing. We've been using a mixture of Superfrost Plus (Fisher), Basix (Fisher), and Platinum line (Mercedes Medical) all with good results. The 'Basix' seems to be a good all-around non-charged slide that is affordable. Hope this helps! Mike Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | Commonwealth Health Laboratory Services | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484 -Original Message- From: Nancy Schmitt [mailto:nancy_schm...@pa-ucl.com] Sent: Monday, January 28, 2019 12:10 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] glass slides Hello- We are seeing some warping with glass slides from Leica. Looking for recommendation on what you are currently using? Thank you Nancy Schmitt MLT, HT(ASCP) Pathology Support Services Manager United Clinical Laboratories NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ** Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cameras in the gross room
Dear Miranda Giorgi: What is the purpose to have cameras, especially at the embedding station? What kind of cameras do you use? Thank you, Izak Dimenstein ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
