[Histonet] Histotech opening in Virginia
Hi Histonetters! Happy Monday - I hope you had a great weekend! I work at K.A. Recruiting specializing in the placement of healthcare professionals into permanent and full-time positions nationwide, and I am currently working with a lab in *Roanoke County, Virginia* that is looking to hire a Histotech for their M-F 4am-12:30pm shift. If you are interested, could you send me a resume and a good time to get in touch? They are looking to start the interview process asap so I would love to give you more information. I also have some other openings, if you or anyone you know may be interested let me know!! Central Georgia - Histotech x 2 openings (days 7:30a-4p and a morning 3a-1130a) Rockford, IL area- Histotech Chicago, IL area - Histotech x 2 openings (midnight ) anatomic pathology Northern Montana - Histotech Charlotte, NC area - Histotech North Dakota - Histology Lead (Monday-Friday day shift) White Plains, NY - Grossing Tech Nashville, TN area - Grosser (evening/ night shift) Again all of my positions are permanent and my clients offer great compensation and benefits, as well as relocation assistance and sign on bonuses! Sincerely, Katherine Marano *K.A. Recruiting, Inc.* Your Partner in Healthcare Recruiting 10 Post Office Square, 8th Floor So. Boston, MA 02109 P: (617) 746-2750 F: (617) 507-8009 kather...@ka-recruiting.com http://www.ka-recruiting.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] DAB enhaced with Nickel Sulfate Ammonium
Better try another counterstaining. Will be easier in the long run.René On Monday, August 5, 2019, 01:08:38 PM EDT, Alonso Martínez Canabal via Histonet wrote: Good Afternoon. It is great to be in this list again. My first e-mail is to ask your advise about a little problem that we have in the lab. We are doing immunohistochemistry in brain tissue using DAB enhanced with nickel ammonium sulfate. The result is great, with a very clean signal in 50um thickness brain sections. Our problem is that later we do some counterstaining with methyl green. It is purified with chorophorm and in pH 4.2 in Acetate buffer. We clearly see the green nuclei and the immunoreactive cells, but apparently, after the counterstaining, the dark-purple-blackish precipitate is gone and we can only see regular brown DAB. What do you think? I wonder if it is the pH of the Methyl green, but I am kind of lost here. Any advise? Thank you very much Dr. Alonso -- Dr. Alonso Martínez Canabal PhD Profesor Asociado "C" Departamento de Biología Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAB enhaced with Nickel Sulfate Ammonium
Good Afternoon. It is great to be in this list again. My first e-mail is to ask your advise about a little problem that we have in the lab. We are doing immunohistochemistry in brain tissue using DAB enhanced with nickel ammonium sulfate. The result is great, with a very clean signal in 50um thickness brain sections. Our problem is that later we do some counterstaining with methyl green. It is purified with chorophorm and in pH 4.2 in Acetate buffer. We clearly see the green nuclei and the immunoreactive cells, but apparently, after the counterstaining, the dark-purple-blackish precipitate is gone and we can only see regular brown DAB. What do you think? I wonder if it is the pH of the Methyl green, but I am kind of lost here. Any advise? Thank you very much Dr. Alonso -- Dr. Alonso Martínez Canabal PhD Profesor Asociado "C" Departamento de Biología Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Influenza A IHC
If anyone is still doing Influenza A IHC testing, could you share your vendor source and catalog number with me? I'm looking to find a better source than what I'm using right now. I don't do it very often (since we have a PCR test) but once in awhile it's requested, and my old antibody just doesn't seem to detect all that it should. Thanks in advance, Jan Shivers Senior Scientist IHC/Histology Section Manager Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu *Confidentiality Notice: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain confidential or privileged information. If you think you have received this message in error, please advise the sender and then delete this message and any attachments immediately.* ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
