Re: [Histonet] pan-TRK or NTRK antibody
VENTANA pan-TRK (EPR17341) Assay Catalog Number: 790-7026 It should not be too difficult to find positive tumour controls since there is a high prevalence of NTRK in some cancers. ie Papillary thyroid cancer and secretary cancers ie secretary breast cancer (75%) Normal testis should stain positive. Endothelial cells should stain positive and will make a great internal control. John Garratt www.ciqc.ca ‐‐‐ Original Message ‐‐‐ On Wednesday, September 11, 2019 12:21 PM, Mark Tarango via Histonet wrote: > While we're on the topic of NTRK, does anyone have a positive case that > they could share? I'm working up the FISH and have probes for NTRK1, 2 & > 3. Any pan-NTRK positive IHC case would be great for detecting by FISH too. > > thanks! > > Mark Tarango > > On Wed, Sep 11, 2019 at 11:23 AM Piche, Jessica via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Good Afternoon, > > Where are people buying the pan-TRK or NTRK antibody from? Pre-dilute > > preferred. Thank you in advance and have a great day! > > Jessica Piche, HT(ASCP) > > Waterbury Hosptial > > Waterbury, CT 06708 > > > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] pan-TRK or NTRK antibody
While we're on the topic of NTRK, does anyone have a positive case that they could share? I'm working up the FISH and have probes for NTRK1, 2 & 3. Any pan-NTRK positive IHC case would be great for detecting by FISH too. thanks! Mark Tarango On Wed, Sep 11, 2019 at 11:23 AM Piche, Jessica via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Good Afternoon, > > Where are people buying the pan-TRK or NTRK antibody from? Pre-dilute > preferred. Thank you in advance and have a great day! > > Jessica Piche, HT(ASCP) > Waterbury Hosptial > Waterbury, CT 06708 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mallory-Azan stain
Thank you John, As always, a wealth of information. I did do a PAS for him, I am not totally sure he knows exactly what he is looking for. I always learn something from your posts. Betsy Betsy Molinari Sr. Histology Research Technician CV Pathology Research Texas Heart Institute 6770 Bertner Avenue, MC 1-283 Houston, TX 77030 Office: 832-355-6524 | Fax: 832-355-6812 Email: bmolin...@texasheart.org texasheart.org | facebook | twitter -Original Message- From: Colleen Forster via Histonet Sent: Tuesday, September 10, 2019 11:17 AM To: Morken, Timothy Cc: Histonet ; John Kiernan Subject: Re: [Histonet] Mallory-Azan stain *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. Wow John, Great information.,always so much to learn!~ Thank you, Colleen Forster U of MN On Tue, Sep 10, 2019 at 10:55 AM Morken, Timothy via Histonet < histonet@lists.utsouthwestern.edu> wrote: > John, we love it when you "ramble!" It gives us an appreciation for > the history and breadth of histo techniques. > > Tim Morken > Supervisor, Electron Microscopy/Neuromuscular Special Studies > Department of Pathology UC San Francisco Medical Center > > -Original Message- > From: John Kiernan via Histonet > [mailto:histonet@lists.utsouthwestern.edu] > > Sent: Tuesday, September 10, 2019 8:43 AM > To: 'Histonet@lists.utsouthwestern.edu'; Betsy Molinari > Subject: Re: [Histonet] Mallory-Azan stain > > Mallory's (easy) and AZAN staining (difficult) are different methods! > > Frank B. Mallory's trichrome stain (Journal of Medical Research 13: > 113-136, 1905) is the earliest and one of the simplest of its kind: > acid fuchsine followed by a solution containing orange G, aniline blue > and phosphotungstic acid (PTA). Martin Heidenhain's trichrome is > usually called AZAN (from Azokarmin and Anilinblau, the German names > of two of the dyes used. I've not read his original 1916 publiication, > but a very thorough account was given by Manfred Gabe in his 1976 > Histological Techniques book (ISBN 3540901620), pp. 219-223. I used > this quite a bit in the 1990s mostly on paraffin sections of Bouin-fixed > decalcified rats' > heads. It is a 15-step procedure taking >2 hours and it includes two > critical differentiations requiring careful microscopic control. > Instructions based on my experiences can be found in Histological and > Histochemical Methods (5th ed., 2015, pp.198-200). > > AZAN gives a wider range of colours than Mallory's or Masson's > trichrome or the various one-step trichromes (Cason, Gomori, Gabe). > The related Romeis "cresazan" procedure was used to identify at least > 6 anterior pituitary cell-types until the 1950s when more rational > histochemically based stains were introduced by Adams, Herlant, Pearse and > others. > Nowadays, immunostainng accurately shows the hormones in pituitary > cells, but much more expensively. > > All trichromes give poor results after simple fixation in neutral > formaldehyde. Bouin or (better) a mercuric chloride-containing > fixative is needed. Zinc-formalin is probably also OK. (I haven't > tried it myself for this purpose). If material fixed in NBF must be > used, immerse hydrated paraffin sections in saturated aqueous picric > acid either for 2h at 56-60C or overnight at room temperature, then wash well > in water before staining. > (Bouin's fluid is often used, but its ingredients other than picric > acid are unnecessary.) Experiments are needed to learn the mechanism > of this "rescue" of staining properties of sections > formaldehyde-fixed tissue, which is sometimes wrongly called > "mordanting". My guess is that it's comparable to antigen retrieval. > It has been claimed that citrate buffer is just as good, though the photos > are unconvincing (J. Histotechnol. 26, 133). > > It should be possible to identify Purkinje fibres with any staining > method that shows nuclei and myofibrils, such as H&E or a trichrome > method simpler than AZAN. A glycogen stain such as PAS might show this > substance in the otherwise pale areas around the central nuclei of > Purkinje fibres. I suggest persuading your researcher to let you try > something simpler before attempting Heidenhain's AZAN. Wheater's > Functional Histology has a nice photomicrograph of a section stained > with H&E and for endocardial elastin (looks like orcein). > > Enough rambling! > John Kiernan > Anatomy & Cell Biology > University of Western Ontario > London, Canada > = = = > > > From: Betsy Molinari via Histonet > Sent: 09 September 2019 10:53 > To: 'Histonet@lists.utsouthwestern.edu' > > > Subject: [Histonet] Mallory-Azan stain > > Hi histonetters, > I have a researcher that wants to stain Purkinje fibers and has > requested a Mallory-Azan stain. > I have no experience with this stain. I have looked online for > info
[Histonet] pan-TRK or NTRK antibody
Good Afternoon, Where are people buying the pan-TRK or NTRK antibody from? Pre-dilute preferred. Thank you in advance and have a great day! Jessica Piche, HT(ASCP) Waterbury Hosptial Waterbury, CT 06708 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] A little temperture monitoring debate
We had our Joint Commission inspection today (no deficiencies!) and the inspector was pressing us to do temperature monitoring in a different way. For chemicals in fridges and freezers we use the manufacturer recommendation of temperature range to set our ranges. Typically for a fridge it is 2C to 8C. That seems pretty standard from datasheets I have. So we set our range at 2-8 on our automated system and then have a 30 min delay if it goes over before an alert sounds. That is to prevent spurious out of range alerts just because someone opened the door for a bit longer than usual. And reagents are not going to warm up instantly either. The probe is in a liquid bottle so will warm up time will be similar to a reagent. Anyway, he thought we should set the ranges narrower so it alerts before it reaches the out of range mark. He felt that if the fridge goes out of range for any time AT ALL then we need to prove all the reagents are still good. He was satisfied with daily control review of immuno stains and said that would prove the reagents work. But I also pointed out to him that we take the diluted reagents out of the fridge and have them on the stainer for up to 8 hours every day at room temperature with no problems. He didn't really have an answer to that but said the manufacturer should consider that in their literature. We don't have too many alerts and those that do occur are usually due to a door not closed are resolved quickly. I'm wondering what others think and do. We had debate this internally when we set up the automated system and considered wider ranges to avoid too many out of range alerts due to opening the doors many times daily, but never considered narrower ranges. We decided to go with the manufacturer ranges in order to be consistent and not have to defend whatever arbitrary narrow or wide range we picked. At least with the manufacturer recommendations we have something on paper to point to. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Detecting copper in H&E staining?
Perhaps they did an orcein stain with H&E counterstain? That would make the copper associated protein a dark red-brown colour but with other components looking like an H&E. Penny Marr Senior BMS C/- Histology Conquest Hospital St Leonards-on-Sea TN37 7RD penny.m...@nhs.net (01424) 758023 -Original Message- From: Jennifer Phinney [mailto:jh...@vet.k-state.edu] Sent: 10 September 2019 15:57 To: HistoNet Subject: [Histonet] Detecting copper in H&E staining? Hello all, I am in a veterinary histology lab. One of my Pathologists mentioned that at two of the locations he'd been at previously (Ithaca, NY and Saskatoon, Canada) copper deposits stained in the routine H&E slides. Is anyone aware of how this could have been achieved? I assume it would either have to be an additive in the eosin or perhaps something naturally occurring in the tap water rinses? Thanks for any assistance, Jennifer Phinney QIHC Histology Laboratory Administrator KSVDL This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in relation to its contents. To do so is strictly prohibited and may be unlawful. Thank you for your co-operation. NHSmail is the secure email and directory service available for all NHS staff in England and Scotland. NHSmail is approved for exchanging patient data and other sensitive information with NHSmail and other accredited email services. For more information and to find out how you can switch, https://portal.nhs.net/help/joiningnhsmail ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
