Re: [Histonet] Permanent mountant for Oil Red O

[B kB via Histonet]

Hi Christopher,

You van try Imsol-mount
Vendors are for example Sigmaaldrich or VWR .

After a staining, airdry the solide. Cover with mislopen-mount and let this
dry for a while ( no problem with higher temps). After that, You can cover
it with glass or tape.

Goed luck,
Bert klein brink
Gelre Hospital, Apeldoorn, Netherlands



Op ma 16 sep. 2019 om 06:04 schreef Hagon, Christopher (Health) via
Histonet 

> UNCLASSIFIED
>
> Hello Histonetters,
>
> We do Oil Red O stains on frozen section post mortem tissue, and need to
> find a permanent aqueous mounting medium. We used to use the Thermo-Fisher
> Perm-mount, but can't seem to get it anymore. As they are looking for fat
> deposits, we can't use any solvent based solutions, and the only aqueous
> ones we've found aren't permanent. We have to send the slides off site
> after staining, so the chances of the coverslip moving in transport is
> fairly high.
>
> Does anyone else have this issue and what did you end up using?
>
> Thanks in advance,
>
> Chris Hagon | Senior Scientist, Anatomical Pathology
> ACT Pathology | health.act.gov.au
> Phone (02) 5124 2874
>
>
>
>
>
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[Histonet] instruments needed

[Blanca Lopez via Histonet]

It's time to updated the lab so I'm looking for those items but they have to be 
new please!
If you are or know vendors please sent me an email.
What are your recommendations?
Any combos that you can make for me please? Quotes or prices asap please
thanks

Water bath
Embedding center
Processor
Microtome
Racks and containers used or not for a autostainer XL Leica

thanks


Blanca Lopez HT (ASCP)cm
Senior Histotechnologist
UT Southwestern Medical Center
Harold C. Simmons Comprehensive Cancer Center
UTSTR Biorepository Tissue Lab
6000 Harry Hines Blvd NB5.102
Dallas, Texas 75390
214-648-7598
blanca.lo...@utsouthwestern.edu





UT Southwestern


Medical Center



The future of medicine, today.

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