date:20190925

Re: [Histonet] Flammable Sprays in Cryostats

2019-09-25 Thread P Sicurello via Histonet

It's not safe to use any type of spray in a cryostat.  It creates aerosols
of who knows what (tuberculosis) from potentially infectious patient
biopsies.  I would not recommend it, just as a universal precaution.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



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On Wed, Sep 25, 2019 at 7:16 PM jasonhauser71--- via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> There are non flammable sprays available. We got the recall notice and
> switched our old inventory out. I found 3 different histology specific
> sprays. The electronic industry offers some as well. Another option is just
> using LN2 .Jason HauserSenior Histology TechnicianThe South Bend ClinicSent
> from my T-Mobile 4G LTE device-- Original message--From: AkemiDate:
> Wed, Sep 25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re:
> [Histonet] Flammable Sprays in CryostatsWe are purchasing two of their
> newer UV cryostats and they were adamant that we use a non flammable
> freezing spray because it can damage the UV unit and have potential for
> exploding.
>
> Akemi Allison
>
> Sent from my iPhone
>
> > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet  wrote:
> >
> > I just received a letter from Leica Biosystems where they are
> prohibiting the usage of flammable
> > freezing sprays in their cryostats.  What are others using in their
> cryostats to instantly freeze specimens?
> >
> > Thank you.
> >
> >
> > Deanne Knutson
> > Supervisor
> > Anatomic Pathology
> > dknut...@primecare.org
> >
> >
> >
> >
> >
> >
> >  
> > This email may include confidential and privileged information. If this
> is not intended for your use, please destroy immediately and contact the
> sender of the message.
> >
> > This email and attachments contain information that may be confidential
> or privileged. If you are not the intended recipient, notify the sender at
> once and delete this message completely from your information system.
> Further use, disclosure, or copying of information contained in this email
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> of privilege or other confidentiality protections.
> > ___
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>
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Re: [Histonet] Flammable Sprays in Cryostats

2019-09-25 Thread jasonhauser71--- via Histonet

There are non flammable sprays available. We got the recall notice and switched 
our old inventory out. I found 3 different histology specific sprays. The 
electronic industry offers some as well. Another option is just using LN2 
.Jason HauserSenior Histology TechnicianThe South Bend ClinicSent from my 
T-Mobile 4G LTE device-- Original message--From: AkemiDate: Wed, Sep 
25, 2019 10:05 AMTo: Knutson, Deanne;Histonet;Cc: Subject:Re: [Histonet] 
Flammable Sprays in CryostatsWe are purchasing two of their newer UV cryostats 
and they were adamant that we use a non flammable freezing spray because it can 
damage the UV unit and have potential for exploding.

Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet  wrote:
> 
> I just received a letter from Leica Biosystems where they are prohibiting the 
> usage of flammable
> freezing sprays in their cryostats.  What are others using in their cryostats 
> to instantly freeze specimens?
> 
> Thank you.
> 
> 
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknut...@primecare.org
> 
> 
> 
> 
> 
> 
>  
> This email may include confidential and privileged information. If this is 
> not intended for your use, please destroy immediately and contact the sender 
> of the message.
> 
> This email and attachments contain information that may be confidential or 
> privileged. If you are not the intended recipient, notify the sender at once 
> and delete this message completely from your information system. Further use, 
> disclosure, or copying of information contained in this email is not 
> authorized, and any such action should not be construed as a waiver of 
> privilege or other confidentiality protections.
> ___
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Re: [Histonet] Freezing spray in cryostats

2019-09-25 Thread Akemi via Histonet

I make my tech’s wear PPE’s, goggles/masks, gloves and N95 masks for potential 
TB and regular masks for normal tissue.
Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 1:00 PM, Charles Riley via Histonet 
>  wrote:
> 
> I have to agree with Terri. I also believe that CAP is going to be putting
> in regulations forbidding the use of freeze sprays in cryostats soon (at
> least I think I heard a rumor about this).
> 
> Anyway another useful item is liquid nitrogen if you really need a quick
> freeze. Dip a brush into the liquid and lightly apply to the specimen. Not
> always recommended as could cause artifacts.
> 
> On Wed, Sep 25, 2019 at 1:37 PM Terri Braud via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> My question is why would you ever want to use an aerosol in the cryostat,
>> taking a chance on aerosolizing some nasty bug?
>> Is the 30 seconds you save in using freezing spray worth the exposure to
>> TB or goodness-knows-what?
>> We use a simple metal heat extractor (comes standard in our Leicas) and
>> place it on top of the freezing block to accelerate the process.  We never
>> have issues with turn around times.
>> For me, no freezing spray.  Ever.
>> 
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>> 
>> -Original Message-
>> From: histonet-requ...@lists.utsouthwestern.edu [mailto:
>> histonet-requ...@lists.utsouthwestern.edu]
>> Sent: Wednesday, September 25, 2019 1:00 PM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: Histonet Digest, Vol 190, Issue 18
>> 
>> CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links
>> or opening attachments.
>> 
>> Send Histonet mailing list submissions to
>>histonet@lists.utsouthwestern.edu
>> 
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>>histonet-ow...@lists.utsouthwestern.edu
>> 
>> When replying, please edit your Subject line so it is more specific
>> than "Re: Contents of Histonet digest..."
>> 
>> 
>> Today's Topics:
>> 
>>   1. Flammable Sprays in Cryostats (Knutson, Deanne)
>>   2. Re: Flammable Sprays in Cryostats (Akemi)
>>   3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
>>   4. Re: Flammable Sprays in Cryostats (Ingles Claire)
>> 
>> 
>> --
>> 
>> Message: 1
>> Date: Wed, 25 Sep 2019 07:50:44 -0500
>> From: "Knutson, Deanne" 
>> To: "'histonet@lists.utsouthwestern.edu'"
>>
>> Subject: [Histonet] Flammable Sprays in Cryostats
>> Message-ID:
>><
>> 1e0e2b14c709174b8ac2be0ae7f768330159b5756...@exchange2k7.staprimecare.org>
>> 
>> Content-Type: text/plain; charset="us-ascii"
>> 
>> I just received a letter from Leica Biosystems where they are prohibiting
>> the usage of flammable
>> freezing sprays in their cryostats.  What are others using in their
>> cryostats to instantly freeze specimens?
>> 
>> Thank you.
>> 
>> 
>> Deanne Knutson
>> Supervisor
>> Anatomic Pathology
>> dknut...@primecare.org
>> 
>> 
>> 
>> 
>> 
>> 
>>  
>> This email may include confidential and privileged information. If this is
>> not intended for your use, please destroy immediately and contact the
>> sender of the message.
>> 
>> This email and attachments contain information that may be confidential or
>> privileged. If you are not the intended recipient, notify the sender at
>> once and delete this message completely from your information system.
>> Further use, disclosure, or copying of information contained in this email
>> is not authorized, and any such action should not be construed as a waiver
>> of privilege or other confidentiality protections.
>> 
>> 
>> --
>> 
>> Message: 2
>> Date: Wed, 25 Sep 2019 09:05:20 -0500
>> From: Akemi 
>> To: "Knutson, Deanne" , Histonet
>>
>> Subject: Re: [Histonet] Flammable Sprays in Cryostats
>> Message-ID: <19db25eb-9944-406b-917c-0878d6f15...@gmail.com>
>> Content-Type: text/plain;   charset=us-ascii
>> 
>> We are purchasing two of their newer UV cryostats and they were adamant
>> that we use a non flammable freezing spray because it can damage the UV
>> unit and have potential for exploding.
>> 
>> Akemi Allison
>> 
>> Sent from my iPhone
>> 
>>> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet <
>> histonet@lists.utsouthwestern.edu> wrote:
>>> 
>>> I just received a letter from Leica Biosystems where they are
>> prohibiting the usage of flammable
>>> freezing sprays in their cryostats.  What are others using in their
>> cryostats to instantly f

Re: [Histonet] Freezing spray in cryostats

2019-09-25 Thread Charles Riley via Histonet

I have to agree with Terri. I also believe that CAP is going to be putting
in regulations forbidding the use of freeze sprays in cryostats soon (at
least I think I heard a rumor about this).

Anyway another useful item is liquid nitrogen if you really need a quick
freeze. Dip a brush into the liquid and lightly apply to the specimen. Not
always recommended as could cause artifacts.

On Wed, Sep 25, 2019 at 1:37 PM Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> My question is why would you ever want to use an aerosol in the cryostat,
> taking a chance on aerosolizing some nasty bug?
> Is the 30 seconds you save in using freezing spray worth the exposure to
> TB or goodness-knows-what?
> We use a simple metal heat extractor (comes standard in our Leicas) and
> place it on top of the freezing block to accelerate the process.  We never
> have issues with turn around times.
> For me, no freezing spray.  Ever.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
>
> -Original Message-
> From: histonet-requ...@lists.utsouthwestern.edu [mailto:
> histonet-requ...@lists.utsouthwestern.edu]
> Sent: Wednesday, September 25, 2019 1:00 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 190, Issue 18
>
> CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links
> or opening attachments.
>
> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. Flammable Sprays in Cryostats (Knutson, Deanne)
>2. Re: Flammable Sprays in Cryostats (Akemi)
>3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
>4. Re: Flammable Sprays in Cryostats (Ingles Claire)
>
>
> --
>
> Message: 1
> Date: Wed, 25 Sep 2019 07:50:44 -0500
> From: "Knutson, Deanne" 
> To: "'histonet@lists.utsouthwestern.edu'"
> 
> Subject: [Histonet] Flammable Sprays in Cryostats
> Message-ID:
> <
> 1e0e2b14c709174b8ac2be0ae7f768330159b5756...@exchange2k7.staprimecare.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> I just received a letter from Leica Biosystems where they are prohibiting
> the usage of flammable
> freezing sprays in their cryostats.  What are others using in their
> cryostats to instantly freeze specimens?
>
> Thank you.
>
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknut...@primecare.org
>
>
>
>
>
>
>   
> This email may include confidential and privileged information. If this is
> not intended for your use, please destroy immediately and contact the
> sender of the message.
>
> This email and attachments contain information that may be confidential or
> privileged. If you are not the intended recipient, notify the sender at
> once and delete this message completely from your information system.
> Further use, disclosure, or copying of information contained in this email
> is not authorized, and any such action should not be construed as a waiver
> of privilege or other confidentiality protections.
>
>
> --
>
> Message: 2
> Date: Wed, 25 Sep 2019 09:05:20 -0500
> From: Akemi 
> To: "Knutson, Deanne" , Histonet
> 
> Subject: Re: [Histonet] Flammable Sprays in Cryostats
> Message-ID: <19db25eb-9944-406b-917c-0878d6f15...@gmail.com>
> Content-Type: text/plain;   charset=us-ascii
>
> We are purchasing two of their newer UV cryostats and they were adamant
> that we use a non flammable freezing spray because it can damage the UV
> unit and have potential for exploding.
>
> Akemi Allison
>
> Sent from my iPhone
>
> > On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > I just received a letter from Leica Biosystems where they are
> prohibiting the usage of flammable
> > freezing sprays in their cryostats.  What are others using in their
> cryostats to instantly freeze specimens?
> >
> > Thank you.
> >
> >
> > Deanne Knutson
> > Supervisor
> > Anatomic Pathology
> > dknut...@primecare.org
> >
> >
> >
> >
> >
> >
> >  
> > This email may include confidential and privileged information. If this
> is not intended for your use, please destroy immediately and contact the
> sender of the me

Re: [Histonet] Freezing spray in cryostats

2019-09-25 Thread Terri Braud via Histonet

My question is why would you ever want to use an aerosol in the cryostat, 
taking a chance on aerosolizing some nasty bug?
Is the 30 seconds you save in using freezing spray worth the exposure to TB or 
goodness-knows-what?  
We use a simple metal heat extractor (comes standard in our Leicas) and place 
it on top of the freezing block to accelerate the process.  We never have 
issues with turn around times.
For me, no freezing spray.  Ever.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
[mailto:histonet-requ...@lists.utsouthwestern.edu] 
Sent: Wednesday, September 25, 2019 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 190, Issue 18

CAUTION: This is an EXTERNAL EMAIL. Stop and think before clicking links or 
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You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."

Today's Topics:

   1. Flammable Sprays in Cryostats (Knutson, Deanne)
   2. Re: Flammable Sprays in Cryostats (Akemi)
   3. Re: Flammable Sprays in Cryostats (Hannen, Valerie)
   4. Re: Flammable Sprays in Cryostats (Ingles Claire)

--

Message: 1
Date: Wed, 25 Sep 2019 07:50:44 -0500
From: "Knutson, Deanne" 
To: "'histonet@lists.utsouthwestern.edu'"

Subject: [Histonet] Flammable Sprays in Cryostats
Message-ID:

<1e0e2b14c709174b8ac2be0ae7f768330159b5756...@exchange2k7.staprimecare.org>

Content-Type: text/plain; charset="us-ascii"

I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable
freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?

Thank you.

Deanne Knutson
Supervisor
Anatomic Pathology
dknut...@primecare.org

This email may include confidential and privileged information. If this is not 
intended for your use, please destroy immediately and contact the sender of the 
message.

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
and delete this message completely from your information system. Further use, 
disclosure, or copying of information contained in this email is not 
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privilege or other confidentiality protections.

--

Message: 2
Date: Wed, 25 Sep 2019 09:05:20 -0500
From: Akemi 
To: "Knutson, Deanne" , Histonet

Subject: Re: [Histonet] Flammable Sprays in Cryostats
Message-ID: <19db25eb-9944-406b-917c-0878d6f15...@gmail.com>
Content-Type: text/plain;   charset=us-ascii

We are purchasing two of their newer UV cryostats and they were adamant that we 
use a non flammable freezing spray because it can damage the UV unit and have 
potential for exploding.

Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet 
>  wrote:
> 
> I just received a letter from Leica Biosystems where they are prohibiting the 
> usage of flammable
> freezing sprays in their cryostats.  What are others using in their cryostats 
> to instantly freeze specimens?
> 
> Thank you.
> 
> 
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknut...@primecare.org
> 
> 
> 
> 
> 
> 
>  
> This email may include confidential and privileged information. If this is 
> not intended for your use, please destroy immediately and contact the sender 
> of the message.
> 
> This email and attachments contain information that may be confidential or 
> privileged. If you are not the intended recipient, notify the sender at once 
> and delete this message completely from your information system. Further use, 
> disclosure, or copying of information contained in this email is not 
> authorized, and any such action should not be construed as a waiver of 
> privilege or other confidentiality protections.
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--

Message: 3
Date: Wed, 25 Sep 2019 15:49:00 +
From: "Hannen, Valerie"

[Histonet] job search in Chicago area

2019-09-25 Thread Margaryan, Naira via Histonet

Hello histonetters,

Please share this email with your colleagues.
I am in a job searching process in Chicago area to be close to my family as my 
appointment in West Virginia University Research Corporation, Morgantown, WV is 
coming to an end since my long-standing supervisor is closing her research 
laboratory.

I have had the privilege of establishing a Research Histology Core in Stanley 
Manne Children's Research Institute, Chicago, IL as well as a Research 
Histology Service in West Virginia University Research Corporation, Morgantown, 
WV, where I have provided histology services to scientists in various 
disciplines to support their research projects. I have also trained students 
enrolled in health science programs in the areas of routine histology and 
animal work.

My experience in cancer research with background in histology and in veterinary 
medicine has given me a unique opportunity to translate my knowledge from basic 
science to clinically relevant applications for diagnostic, prognostic and 
therapeutic development as they relate to cancer. My contributions to various 
research projects have included in vitro and in vivo work of predictive cancer 
biomarkers. I have been involved in testing novel humanized targeted 
antibody-based therapy in the treatment of human cancer in xenograft mouse 
models.

I am confident that my skills and experience would allow me to be a great fit 
for the research laboratory where I could provide excellent support for a wide 
range of scientific projects. I am a fast learner, a hard worker and a strong 
team player.
Furthermore, I am able to transfer a DAKO autostainer and an H&E station as 
part of my relocation.

My Resume and Curriculum Vitae are available by request and provide the more 
comprehensive list of my qualifications and accomplishments. I am very 
interested in meeting to discuss how I can best contribute to the work in 
research laboratory.

Sincerely,
Naira Margaryan

Naira V. Margaryan, D.V.M., Ph.D.
Senior Research Scientist
West Virginia University
Robert C. Byrd Health Sciences Center
Department of Biochemistry
PO Box 9142
200 Erma Byrd Biomedical Research Bldg.
108 Biomedical Road
Morgantown, WV 26506
Tel: 304-293-2213
naira.margar...@hsc.wvu.edu

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Re: [Histonet] Flammable Sprays in Cryostats

2019-09-25 Thread Ingles Claire via Histonet

Liquid Nitrogen guns.
Claire

From: Knutson, Deanne via Histonet 
Sent: Wednesday, September 25, 2019 7:50 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Flammable Sprays in Cryostats

WARNING: This email appears to have originated outside of the UW Health email 
system.
DO NOT CLICK on links or attachments unless you recognize the sender and know 
the content is safe.

I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable
freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?

Thank you.

Deanne Knutson
Supervisor
Anatomic Pathology
dknut...@primecare.org

This email may include confidential and privileged information. If this is not 
intended for your use, please destroy immediately and contact the sender of the 
message.

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Re: [Histonet] Flammable Sprays in Cryostats

2019-09-25 Thread Hannen, Valerie via Histonet

We received the same letter months ago.  We changed to a non-flammable spray.  
It is called STATFREEZE, we purchase it from STATLAB.  The catalog number is 
SL202/134a.  Be careful if you order it because they also sell a product called 
STATFREEZE that is flammable. 



Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: Knutson, Deanne via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, September 25, 2019 8:51 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL Sender] [Histonet] Flammable Sprays in Cryostats


This message came from an external source. Please do not click links or open 
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Begin Original Message:

--
I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable

freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?



Thank you.





Deanne Knutson

Supervisor

Anatomic Pathology

dknut...@primecare.org













  

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Re: [Histonet] Flammable Sprays in Cryostats

2019-09-25 Thread Akemi via Histonet

We are purchasing two of their newer UV cryostats and they were adamant that we 
use a non flammable freezing spray because it can damage the UV unit and have 
potential for exploding.

Akemi Allison

Sent from my iPhone

> On Sep 25, 2019, at 7:50 AM, Knutson, Deanne via Histonet 
>  wrote:
> 
> I just received a letter from Leica Biosystems where they are prohibiting the 
> usage of flammable
> freezing sprays in their cryostats.  What are others using in their cryostats 
> to instantly freeze specimens?
> 
> Thank you.
> 
> 
> Deanne Knutson
> Supervisor
> Anatomic Pathology
> dknut...@primecare.org
> 
> 
> 
> 
> 
> 
>  
> This email may include confidential and privileged information. If this is 
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> of the message.
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[Histonet] Flammable Sprays in Cryostats

2019-09-25 Thread Knutson, Deanne via Histonet

I just received a letter from Leica Biosystems where they are prohibiting the 
usage of flammable
freezing sprays in their cryostats.  What are others using in their cryostats 
to instantly freeze specimens?

Thank you.


Deanne Knutson
Supervisor
Anatomic Pathology
dknut...@primecare.org






  
This email may include confidential and privileged information. If this is not 
intended for your use, please destroy immediately and contact the sender of the 
message.

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
and delete this message completely from your information system. Further use, 
disclosure, or copying of information contained in this email is not 
authorized, and any such action should not be construed as a waiver of 
privilege or other confidentiality protections.
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