Re: [Histonet] stored in 80% isopropanol

[Hobbs, Carl via Histonet]

If you have a processer using IMS ( or the more expensive ethanol)
take tissues from that 80% IPA ( iso Propyl alcohol) to a fresh 80% 
IMS/ethanol, whichever you use as std and continue as normal 
( you have no idea if the specimens were taken from water/PFA directly into 80% 
IPA? Thus there will be more than 20% water in there)
IMS/IPA/EtOH are all compatible for Pwax processing, where a std clearing agent 
is used ( eg: Xylene, Histoclear)
Sure, you can as suggested use increasing concs IPA and go directly into wax, 
bypassing the "clearing" stage but, imho longer Pwax times are needed as IPA is 
not miscible in Pwax unless at the high temp used.no big deal.
I have used this method in the past, with success
Tho I have to admit that I still for my sins, find that IMS then Xylene gives 
the best final Pwax block!
If others disagree, I'd be grateful to know what their safer equally as good 
clearing agent alternatives are and the cost/block compared with using xylene.
Cost/benefit?

Best wishes


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 

020 7848 6813
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[Histonet] Leica Bond Rx covertile cleaning question?

[Katherine Bummer via Histonet]

Hello Histonetters,

I wanted to find out how you are cleaning your covertiles that are used for 
chromogenic & Immunofluourescent IHC as well as RNAscope?

Thank you!

Kate

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Re: [Histonet] stored in 80% isopropanol

[Betsy Molinari via Histonet]

Yes, but I plan to put them in my processor starting at 95% ETOH and continue 
with my regular processing. I am assuming there should be no issues with that 
correct?

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
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From: Willis, Donna G 
Sent: Wednesday, June 3, 2020 5:43 PM
To: Betsy Molinari ; 
'Histonet@lists.utsouthwestern.edu' 
Subject: Re: stored in 80% isopropanol


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80% Isopropanol has 20% water. You will still have water in the tissue.

Donna Willis
Anatomic Pathology Manager
Baylor University Medical Center
Baylor Scott&White Health
214-820-2465 office

From: Betsy Molinari via Histonet 
mailto:histonet@lists.utsouthwestern.edu>>
Sent: Wednesday, June 3, 2020 1:39:37 PM
To: 'Histonet@lists.utsouthwestern.edu' 
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Subject: {EXTERNAL} [Histonet] stored in 80% isopropanol


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Hi,
If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 
% isopropanol  can the tissue be processed as if it was in ETOH? What are the 
effects to the tissue stored in isopropanol?
Thanks,
Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston, TX 77030

832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
texasheart.org
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 >

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Re: [Histonet] stored in 80% isopropanol

[Betsy Molinari via Histonet]

That is very interesting. Thank you for mentioning that!


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812 
Email: bmolin...@texasheart.org
texasheart.org  | facebook 
 | twitter 
-Original Message-
From: Gilchrist, Eric P  
Sent: Wednesday, June 3, 2020 3:14 PM
To: Tracy Pheneger ; Betsy Molinari 
; Porter, Amy 
Subject: RE: stored in 80% isopropanol

*** Important *** This email is not from Texas Heart Institute. Only click 
links or open attachments you know are safe.




If I am not mistaken, Thermo-Fisher's "Reagent Alcohols" are all Isopropanol 
based.  When we have providers who do not have a biopsy kit available and have 
a specimen I usually advise them to put the tissue in 70% isopropanol to fix 
and store and then overnight a biopsy kit to them.

Eric Gilchrist | Supervisor Oral & Maxillofacial Pathology University of North 
Carolina Adams School of Dentistry P 919.537.3153 | F 919.843.6508



-Original Message-
From: Tracy Pheneger via Histonet 
Sent: Wednesday, June 3, 2020 3:00 PM
To: Betsy Molinari ; Porter, Amy ; 
'Histonet@lists.utsouthwestern.edu' 
Subject: Re: [Histonet] stored in 80% isopropanol

Isopropanol processing is routinely used with formalin fixation.
Isopropanol is miscible in paraffin, so you can skip the clearing agents. Just 
run it down to 100% Isopropanol as you would do on a Ethanol processing 
schedule, then straight into paraffin.
Check this out:

https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_14534624_&d=DwIGaQ&c=5rShgjAa2OW9AX8kjI_BFTEsAS2aUiDYBgABvVqRiz0&r=4JF0M5k_UrXYJLzefN3bjagdyUrCioVawjbCC16NNH8&m=Z8lCQROWymUZDTR2-MB-L6oGzMGD3l5tsvfWpQLIcGs&s=yJZyBp1_0VSLcTPQXOs29ban8nT7qsaQzMbA9StpsRA&e=

😊

Tracy

Tracy Pheneger, HT/HTL (ASCP), QIHC (ASCP), LATg (AALAS) Senior Professional 
Research Assistant BioServe Space Technologies University of Colorado c/o 
Kolicko AES Univ of Colorado Rm 224, 3775 Discovery Dr Boulder CO 80309

From: Betsy Molinari via Histonet
Sent: Wednesday, June 3, 2020 12:50 PM
To: Porter, Amy; 
'Histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] stored in 80% isopropanol

I am also in research. I have very little to go on, but I presume it is not 
going to yield good results.


Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
texasheart.org | 
facebook | 
twitter
From: Porter, Amy 
Sent: Wednesday, June 3, 2020 1:47 PM
To: Betsy Molinari 
Subject: Re: stored in 80% isopropanol


*** Important *** This email is not from Texas Heart Institute. Only click 
links or open attachments you know are safe.



Rubbing alcohol - potentially really dehydrated already depending on how 
long???  I am research so no recommendation for clinical.


From: Betsy Molinari via Histonet 
mailto:histonet@lists.utsouthwestern.edu>>
Sent: Wednesday, June 3, 2020 2:39 PM
To: 'Histonet@lists.utsouthwestern.edu' 
mailto:Histonet@lists.utsouthwestern.edu>>
Subject: [Histonet] stored in 80% isopropanol

Hi,
If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80 
% isopropanol  can the tissue be processed as if it was in ETOH? What are the 
effects to the tissue stored in isopropanol?
Thanks,
Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
Houston, TX 77030

832-355-6524 (lab)
832-355-6812 (fax)

Betsy Molinari, HT (ASCP)
Sr. Histology Research Technician
CV Pathology Research

Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030

Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
texasheart.org
 > | 
facebook