Re: [Histonet] Histology text book "bibles"
These last few posts r so wonderful and revealing. Where we came from and all the glorious history. Very nostalgic Dr Azam Sent from my iPhone > On Jul 9, 2020, at 2:44 PM, Hobbs, Carl via Histonet > wrote: > > Good to read of others' xp > I remember the Armed Forces book...good in parts, unlike the Curate's egg! > Disbrey and Rack...wonderfully refreshing. > Exceedingly well-informed person was Brenda! > I reckon she swung the panel in my favour for a Histology Manager > job.YEARS ago! > Imho the forerunner of the best: Kiernan > Sure, neck and neck with Bancroft and Stevensboth game-changers > Pearce...wonderfully instructive yetdense? > What about the 70's "bible"...I forget the English author..dash it! > His surname begins with a C? > Carleton! > The book was THE text at that time ( in UK)...however, I found it rather > didacticno depth > I could be wrong, of course! > I did learn a lot from it...then found it lacing in depth...thirst for Histo > teck knowledge, chuckle > It gave me a seminal Taster for more, however. > Met him at many London Histology discussion meetings...at one of which he > dismisssed me for using DPX mountant > ( cheaper for my lab/quickersetting...close RI , I recall?) > Harrumph, he saiduse natural resin: Canada balsam! > Much closer RIhmm > What does Prof. Kiernan think re DPX v Canada balsam?? > Luverly smell..tho ( C.balsam) > Does anyone use CBalsam still? > Also > sure, Harry Cook has a special place in my Histology career...such a humble > yet...knowledgeable person. > He opened up my mind to things Histological...his depth and breadth of things > Histological was...humbling and mesmeric. > His Carbohydrate booklet was so good for me, when I needed it! > Curious-illy > Always > > Carl > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson CARD > Guys Campus, London Bridge > Kings College London > London > SE1 1UL > > > 020 7848 6813 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology text book "bibles"
Good to read of others' xp I remember the Armed Forces book...good in parts, unlike the Curate's egg! Disbrey and Rack...wonderfully refreshing. Exceedingly well-informed person was Brenda! I reckon she swung the panel in my favour for a Histology Manager job.YEARS ago! Imho the forerunner of the best: Kiernan Sure, neck and neck with Bancroft and Stevensboth game-changers Pearce...wonderfully instructive yetdense? What about the 70's "bible"...I forget the English author..dash it! His surname begins with a C? Carleton! The book was THE text at that time ( in UK)...however, I found it rather didacticno depth I could be wrong, of course! I did learn a lot from it...then found it lacing in depth...thirst for Histo teck knowledge, chuckle It gave me a seminal Taster for more, however. Met him at many London Histology discussion meetings...at one of which he dismisssed me for using DPX mountant ( cheaper for my lab/quickersetting...close RI , I recall?) Harrumph, he saiduse natural resin: Canada balsam! Much closer RIhmm What does Prof. Kiernan think re DPX v Canada balsam?? Luverly smell..tho ( C.balsam) Does anyone use CBalsam still? Also sure, Harry Cook has a special place in my Histology career...such a humble yet...knowledgeable person. He opened up my mind to things Histological...his depth and breadth of things Histological was...humbling and mesmeric. His Carbohydrate booklet was so good for me, when I needed it! Curious-illy Always Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Crystals forming in Hematoxylin
That is the aluminum salt precipitating out. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, July 9, 2020, 11:47:53 AM CDT, Terri Braud via Histonet wrote: Hello Histo Peeps - What are the clear crystals that form in Hematoxylin? We use Richard-Allen 7211. We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom. Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful. It had a few occlusions of purple streaks, but otherwise was clear. Doesn't seem to affect the stain, so I was just curious. Inquisitively yours, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Crystals forming in Hematoxylin
Hi Terri, The crystals form during cold winter months, usually during shipping or can form if the Hematoxylin 7211 is stored in a cold environment. They also form due to the Mordant being put into solution at it's saturation point. The crystals have no adverse effect on the stain itself. If your bottle has a few crystals, just filter it and it should remain crystal free. If there are a lot, you can gently warm it, using a "hot" plate and constant stirring until the crystals go back into solution, then filter it and is should be good to go. I only learned this when I "googled" about it myself. I hope this helps!! Regards, Valerie Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -Original Message- From: Terri Braud via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, July 09, 2020 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL Sender] [Histonet] Crystals forming in Hematoxylin This message came from an external source. Please do not click links or open attachments if unexpected or unusual. Begin Original Message: -- Hello Histo Peeps - What are the clear crystals that form in Hematoxylin? We use Richard-Allen 7211. We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom. Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful. It had a few occlusions of purple streaks, but otherwise was clear. Doesn't seem to affect the stain, so I was just curious. Inquisitively yours, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PaPH0eDnAlaiSnq7Yv-U!vBWHyLqdMq6OOl66sorBVdmBIxsRePjH1moP6w3bOijwraARJ_4VQ8f_A8SaZWSs2RVXMg$ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Crystals forming in Hematoxylin
Hello Histo Peeps - What are the clear crystals that form in Hematoxylin? We use Richard-Allen 7211. We love the stain, but occasionally, when a bottle has been previously opened, we find clear crystalline precipitate forming in the bottom. Today, we had a huge one (over an 2" in greatest diameter) that was quite beautiful. It had a few occlusions of purple streaks, but otherwise was clear. Doesn't seem to affect the stain, so I was just curious. Inquisitively yours, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen tissue
I'm testing a faster Mart 1 for melanoma on Mohs specimens. Its been a while since I've done any frozen tissue IHC stains and I can't remember the fixation to keep it viable for testing the next day, or same day but later on. Will it hold up if fixed in acetone and dried or do I need to refrigerate or freeze? Also how long do they last, I can't remember the protocol. Thanks Anne HT ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
