Re: [Histonet] Histonet Digest, Vol 204, Issue 6
Good morning All, I have had my fair share of nails falling off. I have found sometimes letting the slides air dry over night and staining then the next day helps. Have you tried thus yet? Respectfully Krista On Sat, Nov 14, 2020, 3:00 AM wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > >1. Toenail staining issues (Charles Riley) > > > > -- Forwarded message -- > From: Charles Riley > To: "histonet@lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Fri, 13 Nov 2020 14:51:29 + > Subject: [Histonet] Toenail staining issues > >From time to time, we have issues with our sections of toenail falling > off the slide during staining. > > Our H&E sections usually stain without issue but when we do our special > stains (PASF and GMS) the tissue falls off routinely. > > We use charged slides and if a section falls off, we coat the recut slide > with albumin to help get extra adherence. However sometimes even this does > not help the tissue stick through the whole process (especially on GMS). > > > Does anyone have any additional suggestion we could try to get the > sections to adhere better throughout the process? > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] old microtome LKB ULTRATOME TYPE 4801A
Just got a LKB ULTRATOME TYPE 4801A on my desk . Any chance that one of you have a manual? Would be nice if it still works. Tora ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cerebro
Does anybody have a Leica Biosystems CEREBRO users guide or manual? *John Frazier* *MT(ASCP), MBA* *Lean 6 Six Sigma Black Belt* *Healthcare Consultant* *704-847-0566* *wilfong1...@gmail.com * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Toenail staining issues
We use poly-L-lysine. Works for us. P4707 Sigma-Aldrich Poly-L-lysine solution 0.01%, sterile-filtered, BioReagent, suitable for cell cultur Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786 Fax: 413-322-4790 charles.ba...@baystatehealth.org -Original Message- From: Charles Riley Sent: Friday, November 13, 2020 9:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toenail staining issues >From time to time, we have issues with our sections of toenail falling off the >slide during staining. Our H&E sections usually stain without issue but when we do our special stains (PASF and GMS) the tissue falls off routinely. We use charged slides and if a section falls off, we coat the recut slide with albumin to help get extra adherence. However sometimes even this does not help the tissue stick through the whole process (especially on GMS). Does anyone have any additional suggestion we could try to get the sections to adhere better throughout the process? -- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794- and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Toenail staining issues
>From time to time, we have issues with our sections of toenail falling off the >slide during staining. Our H&E sections usually stain without issue but when we do our special stains (PASF and GMS) the tissue falls off routinely. We use charged slides and if a section falls off, we coat the recut slide with albumin to help get extra adherence. However sometimes even this does not help the tissue stick through the whole process (especially on GMS). Does anyone have any additional suggestion we could try to get the sections to adhere better throughout the process? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
