[Histonet] Floaters
Here is a reference link to floaters. My experience with tissue floaters is that most come from staining baths. However, some manufacturers of stainers claim there is significance with these floaters. My experience is that Pathologists read through the floaters and for the most part they don't mind. My concern for floaters that pose a real threat to an accurate diagnosis come from the block. Created either at grossing with contaminated grossing tools or embedding. https://meridian.allenpress.com/aplm/article/133/6/973/64098/Tissue-Floaters-and-Contaminants-in-the-Histology *John Frazier* *MT(ASCP), MBA* *Lean 6 Six Sigma Black Belt* *Healthcare Consultant* *704-847-0566* *wilfong1...@gmail.com * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Crusting lesions and thick cattle skin
Hi!I'm looking for recommendations for cutting FFPE blocks that contain thick cattle hide/skin and crusting lesions. What do you all use to soften this kind of tissue? Our lab has used Millifex, fabric softener, and nair. We read ammonia may work to rehydrate the tissue enough to get a decent section. Has anyone tried that?Thanks in advance for your help!Rhonda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Floaters
Interesting thread. Thanks for kicking it off. Does anybody have a reference that cites diagnostic errors caused by floaters / contamination from other specimens? John On Mon, Dec 7, 2020 at 9:15 AM, Joe W. Walker, Jr. via Histonet wrote: > Don’t shoot the messenger. :) > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology and Interim Phlebotomy Manager > Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > P 802.747.1790 F 802.747.6525 > joewal...@rrmc.org, www.rrmc.org > > -Original Message- > From: Terri Braud via Histonet > Sent: Monday, December 7, 2020 8:42 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: Re: [Histonet] Floaters > > [External Email] This email originated from outside of the organization. > Think before you click: Don’t click on links, open attachments or respond to > requests for sensitive information if the email looks suspicious or you don’t > recognize the sender. > > 60% of floaters from the water bath? I find that really hard to believe. > The Gephardt and Zarbo CAP study from 1996 showed reported the results of a > Q-Probes study of 275 laboratories and documented a frequency of > contamination of between 0.6% and 2.9%, depending on the study method. Their > study demonstrated the rate of extraneous tissue contamination was higher for > blocks than for slides and higher in a retrospective review than in a > prospective study. So in other words, when people knew they were being > studied, they were more careful and the contamination rate went down, but in > retrospect, the majority of floaters occurred in the blocks, not the water > bath. > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > HNL Labs, Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > Today's Topics: > > 1. "Floaters" in surgical or cytology specimens > (Martha Ward-Pathology) > 2. Re: "Floaters" in surgical or cytology specimens > (Joe W. Walker, Jr.) > > -- > > Message: 1 > Date: Fri, 4 Dec 2020 13:55:20 + > From: Martha Ward-Pathology > To: "histonet@lists.utsouthwestern.edu" > > Subject: [Histonet] "Floaters" in surgical or cytology specimens > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > I am posting this question for a colleague in our Cytology department. How > often do you see floaters on surgical or cytology specimens? Obviously we > would never want to see any type of carryover but is there a standard rate > published somewhere that he can reference? > > Thanks in advance for your help. > > Martha Ward, MT ASCP, QIHC > Manager, Molecular Diagnostics Lab > Wake Forest Baptist Medical Center > > -- > > Message: 2 > Date: Fri, 4 Dec 2020 17:41:11 + > From: "Joe W. Walker, Jr." > To: Martha Ward-Pathology > Cc: "histonet@lists.utsouthwestern.edu" > > Subject: Re: [Histonet] "Floaters" in surgical or cytology specimens > Message-ID: > > > Content-Type: text/plain; charset="utf-8" > > https://urldefense.com/v3/__https://academic.oup.com/ajcp/article/136/5/767/1766314__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYpUvPASA$ > > "Floaters represent a potential source of diagnostic error and occur in 0.01% > to 1.2% of slides. Pick up of floaters from the water bath appears most > common (?60%). Floaters in only 1 level and mismatch with the specimen tissue > type are clues to the extraneous nature of the floater." > > Joe W. Walker, Jr. MS, SCT(ASCP) > Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical > Center > 160 Allen Street, Rutland, VT 05701 > P 802.747.1790 F 802.747.6525 > joewal...@rrmc.org, http://www.rrmc.org > > -Original Message- > From: Martha Ward-Pathology via Histonet > Sent: Friday, December 4, 2020 8:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] "Floaters" in surgical or cytology specimens > > [External Email] This email originated from outside of the organization. > Think before you click: Don?t click on links, open attachments or respond to > requests for sensitive information if the email looks suspicious or you don?t > recognize the sender. > > I am posting this question for a colleague in our Cytology department. How > often do you see floaters on surgical or cytology specimens? Obviously we > would never want to see any type of carryover but is there a standard rate > published somewhere that he can reference? > > Thanks in advance for your help. > > Martha Ward, MT ASCP, QIHC > Manager, Molecular Diagnostics Lab > Wake Forest Baptist Medical Center > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!vtJNIWgmSiJpyLbSct_WD7kUYBMBOk43t6WiqJfWP
Re: [Histonet] Floaters
Don’t shoot the messenger. :) Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewal...@rrmc.org, www.rrmc.org -Original Message- From: Terri Braud via Histonet Sent: Monday, December 7, 2020 8:42 AM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Floaters [External Email] This email originated from outside of the organization. Think before you click: Don’t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don’t recognize the sender. 60% of floaters from the water bath? I find that really hard to believe. The Gephardt and Zarbo CAP study from 1996 showed reported the results of a Q-Probes study of 275 laboratories and documented a frequency of contamination of between 0.6% and 2.9%, depending on the study method. Their study demonstrated the rate of extraneous tissue contamination was higher for blocks than for slides and higher in a retrospective review than in a prospective study. So in other words, when people knew they were being studied, they were more careful and the contamination rate went down, but in retrospect, the majority of floaters occurred in the blocks, not the water bath. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor HNL Labs, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. "Floaters" in surgical or cytology specimens (Martha Ward-Pathology) 2. Re: "Floaters" in surgical or cytology specimens (Joe W. Walker, Jr.) -- Message: 1 Date: Fri, 4 Dec 2020 13:55:20 + From: Martha Ward-Pathology To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] "Floaters" in surgical or cytology specimens Message-ID: Content-Type: text/plain; charset="us-ascii" I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens?Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center -- Message: 2 Date: Fri, 4 Dec 2020 17:41:11 + From: "Joe W. Walker, Jr." To: Martha Ward-Pathology Cc: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] "Floaters" in surgical or cytology specimens Message-ID: Content-Type: text/plain; charset="utf-8" https://urldefense.com/v3/__https://academic.oup.com/ajcp/article/136/5/767/1766314__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak39dSSWFLa5SL1M73A18pgYpUvPASA$ "Floaters represent a potential source of diagnostic error and occur in 0.01% to 1.2% of slides. Pick up of floaters from the water bath appears most common (?60%). Floaters in only 1 level and mismatch with the specimen tissue type are clues to the extraneous nature of the floater." Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewal...@rrmc.org, http://www.rrmc.org -Original Message- From: Martha Ward-Pathology via Histonet Sent: Friday, December 4, 2020 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "Floaters" in surgical or cytology specimens [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens?Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!vtJNIWgmSiJpyLbSct_WD7kUYBMBOk43t6WiqJfWPo6GJv5urHibw3NEp3Ztj3I$ [https://www.rrmc.org/app/files/public/3159/ValesEmailSig2020.jpg] -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!rJ2yq9KcDC2PooORZtJvXi4R8vHOIg5tak
Re: [Histonet] Floaters
60% of floaters from the water bath? I find that really hard to believe. The Gephardt and Zarbo CAP study from 1996 showed reported the results of a Q-Probes study of 275 laboratories and documented a frequency of contamination of between 0.6% and 2.9%, depending on the study method. Their study demonstrated the rate of extraneous tissue contamination was higher for blocks than for slides and higher in a retrospective review than in a prospective study. So in other words, when people knew they were being studied, they were more careful and the contamination rate went down, but in retrospect, the majority of floaters occurred in the blocks, not the water bath. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor HNL Labs, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal Today's Topics: 1. "Floaters" in surgical or cytology specimens (Martha Ward-Pathology) 2. Re: "Floaters" in surgical or cytology specimens (Joe W. Walker, Jr.) -- Message: 1 Date: Fri, 4 Dec 2020 13:55:20 + From: Martha Ward-Pathology To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] "Floaters" in surgical or cytology specimens Message-ID: Content-Type: text/plain; charset="us-ascii" I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens?Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center -- Message: 2 Date: Fri, 4 Dec 2020 17:41:11 + From: "Joe W. Walker, Jr." To: Martha Ward-Pathology Cc: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] "Floaters" in surgical or cytology specimens Message-ID: Content-Type: text/plain; charset="utf-8" https://academic.oup.com/ajcp/article/136/5/767/1766314 "Floaters represent a potential source of diagnostic error and occur in 0.01% to 1.2% of slides. Pick up of floaters from the water bath appears most common (?60%). Floaters in only 1 level and mismatch with the specimen tissue type are clues to the extraneous nature of the floater." Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790 F 802.747.6525 joewal...@rrmc.org, www.rrmc.org -Original Message- From: Martha Ward-Pathology via Histonet Sent: Friday, December 4, 2020 8:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "Floaters" in surgical or cytology specimens [External Email] This email originated from outside of the organization. Think before you click: Don?t click on links, open attachments or respond to requests for sensitive information if the email looks suspicious or you don?t recognize the sender. I am posting this question for a colleague in our Cytology department. How often do you see floaters on surgical or cytology specimens?Obviously we would never want to see any type of carryover but is there a standard rate published somewhere that he can reference? Thanks in advance for your help. Martha Ward, MT ASCP, QIHC Manager, Molecular Diagnostics Lab Wake Forest Baptist Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!I87qwjxLstg3H_X5!vtJNIWgmSiJpyLbSct_WD7kUYBMBOk43t6WiqJfWPo6GJv5urHibw3NEp3Ztj3I$ [https://www.rrmc.org/app/files/public/3159/ValesEmailSig2020.jpg] -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- End of Histonet Digest, Vol 205, Issue 2 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
