Re: [Histonet] Protocol for DAPI staining on paraffin sections

[Alida Bailleul via Histonet]

Dear Carl, thank you so much for checking!
Yes, may I please see some images? And would you mind to share your
specific protocols and the brand also of the DAPI that you bought? (I am
guessing it does not matter too much though).
Thank you very very much, I really appreciate the help.

All the best

Alida

ps: my sections are demineralized bone, it should work the same for any
tissue right? Do you recommend to cut and then stain right away? Or would
it also work on paraffin slides that were cut a few years ago?

On Wed, Jun 9, 2021 at 10:01 PM Hobbs, Carl via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Dear Alida
> my emails to your msu.montan.edu get returned to me as undeliverable
> I have incubated 2 dewaxed sections ( different slides) in HOECHST +/-
> HIER.
> Both stain nuclei very well.
> The HIER-treated section gave a brighter nuclear positivity ( so needed to
> lower exposure time on taking images)
> NSD though
> I can post on Histonet images, if you wish
>
> carl
>
>
>
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge
> Kings College London
> London
> SE1 1UL
>
>
>
> 020 7848 6810
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Dr. Alida M. Bailleul
Associate Research Fellow/Associate Professor
Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy
of Sciences
www.ivpp-avianevolution.com
& Research Associate of Paleontology, Museum of the Rockies, Montana State
University
Google Scholar
 -
ResearchGate

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Re: [Histonet] Protocol for DAPI staining on paraffin sections

[Hobbs, Carl via Histonet]

Dear Alida
my emails to your msu.montan.edu get returned to me as undeliverable
I have incubated 2 dewaxed sections ( different slides) in HOECHST +/- HIER.
Both stain nuclei very well.
The HIER-treated section gave a brighter nuclear positivity ( so needed to 
lower exposure time on taking images)
NSD though
I can post on Histonet images, if you wish

carl



Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 


020 7848 6810
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