Re: [Histonet] Ada's Mail Stain line order

2021-10-26 Thread Dee Wolfe via Histonet 
Hi Anne,

Are they using the term "clearing" loosely, but really mean "rinsing"? The term 
"clearing" typically refers to the step after the alcohol (dehydration) and 
before coverslipping (if using a permanent, solvent based mounting media). Not 
sure why someone would "clear" before the rinse and bluing step in frozen 
section staining.

For paraffin embedded specimens, the slide would need to Deparaffinize (in 
clearing agent-xylene or xylene substitute), dehydrate (graded alcohols—high to 
low), water rinse (preferably DI), stain the nuclei (hematoxylin), water rinse, 
decolorize (acid alcohol-if staining regressively), water rinse(s), bluing, 
water rinses, graded alcohol–low to high, cytoplasmic staining (Eosin), alcohol 
rises, clearing.

For frozen section: 100% alcohol (or other fixative), water rise, hematoxylin 
(usually progressive, so no need to decolorize), bluing, water rinse, etc…

You may choose to skip bluing as tap water can do it. Although for consistency 
it should be kept in (tap water pH can with seasons, water treatment facilty 
processes, etc.)

Dee Wolfe


On Oct 26, 2021, at 12:26 PM, Anne Murvosh via Histonet wrote:

> When doing frozen section staining for Mohs we have DI water and don't need a 
> clearing agent. I am helping set up someone who is using tap water and needs 
> to clear it. They mentioned that they used to clear first, rinse, then use 
> bluing. I thought it was the opposite after heme. Rinse, bluing, rinse, 
> clearing. I don't remember which is first. Actually do you need a bluing if 
> using tap water since that blues better then DI water? Thanks for your input, 
> it's been 10 years since I had to think about this. Anne
> 
> 
> Anne Murvosh
> Histology Technician
> 
> 
> 
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Re: [Histonet] Stain line order

2021-10-26 Thread Mac Donald, Jennifer via Histonet 
That step is known as differentiation.  Clearing involves an organic solvent to 
prepare the slides for coverslipping in a synthetic mounting medium.  
A regressive H&E uses differentiation, usually with 1% acid alcohol.  A 
progressive stain does not.  Some companies use a modified progressive using 
clarifier which is an acid alcohol using acetic acid.

-Original Message-
From: Anne Murvosh  
Sent: Tuesday, October 26, 2021 9:56 AM
To: Mac Donald, Jennifer 
Subject: RE: Stain line order

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

Clear; the tap water makes a blue film on the slides. I think this person used 
to use HCL water. I used clarifier 2 in the past.  Thanks Anne

-Original Message-
From: Mac Donald, Jennifer 
Sent: Tuesday, October 26, 2021 9:49 AM
To: Anne Murvosh 
Subject: RE: Stain line order

Clear or differentiate?

-Original Message-
From: Anne Murvosh via Histonet 
Sent: Tuesday, October 26, 2021 9:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Stain line order

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

When doing frozen section staining for Mohs we have DI water and don't need a 
clearing agent. I am helping set up someone who is using tap water and needs to 
clear it. They mentioned that they used to clear first, rinse, then use bluing. 
I thought it was the opposite after heme. Rinse, bluing, rinse, clearing. I 
don't remember which is first. Actually do you need a bluing if using tap water 
since that blues better then DI water? Thanks for your input, it's been 10 
years since I had to think about this. Anne


Anne Murvosh
Histology Technician



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[Histonet] Stain line order

2021-10-26 Thread Anne Murvosh via Histonet 
When doing frozen section staining for Mohs we have DI water and don't need a 
clearing agent. I am helping set up someone who is using tap water and needs to 
clear it. They mentioned that they used to clear first, rinse, then use bluing. 
I thought it was the opposite after heme. Rinse, bluing, rinse, clearing. I 
don't remember which is first. Actually do you need a bluing if using tap water 
since that blues better then DI water? Thanks for your input, it's been 10 
years since I had to think about this. Anne


Anne Murvosh
Histology Technician



This electronic transmission and any documents accompanying this electronic 
transmission may contain information that is confidential and/or legally 
privileged. The information is intended only for the use of the individual or 
entity named above. If you are not the intended recipient, you are hereby 
notified that any disclosure, copying, distribution or the taking of any action 
in reliance on or regarding the contents of this electronically transmitted 
information is strictly prohibited. If you have received this e-mail in error, 
please notify the sender and delete this message immediately.

HIPAA Confidentiality Notice: The information and documents accompanying this 
e-mail may contain confidential information that is legally privileged and 
protected by federal and state law. The information is intended for use only by 
the entity or individual to whom it is addressed, the authorized recipient. The 
authorized recipient is obligated to maintain the information in a safe, 
secure, and confidential manner. The authorized recipient is prohibited from 
using the information for purposes other than intended, prohibited from 
disclosing the information to any other party unless required to do so by law 
or regulation, and is required to destroy the information after its stated need 
has been fulfilled. If you are in possession of this information, and are not 
the intended recipient, you are hereby notified that any improper disclosure, 
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[Histonet] Histonetters!!! The Florida Society for Histotechnology is having their Fall Meeting virtually. Here is the info.

2021-10-26 Thread Pam Barker via Histonet 
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