Re: [Histonet] Special Stainers
Agilent-Artisan stainers are amazing. You can search old Histonet posts with my explanation as to why! I once had a lot to say about this. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcoo...@chla.usc.edu -Original Message- From: Kara, Phillip via Histonet Sent: Wednesday, February 22, 2023 11:05 AM To: Eddie Martin via Histonet Subject: [Histonet] Special Stainers (EXTERNAL EMAIL) CAUTION: BE CAREFUL WITH THIS MESSAGE* This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-boun...@lists.utsouthwestern.edu. So I am working for a brand new lab that has been open since September. We currently perform all our specials by hand (roughly 7 stains; 3 silvers) and our numbers are starting to shoot up big time on them. Any recommendations on special stainers and kits that go with them? We are a Sakura flag ship lab but we are open to other equipment if the cost is good and the results are great. Phillip Kara BS, HTL(ASCP) : Lead Histology Tech Wise Pathology LLC 609 Medical Center Dr. Decatur TX. 76234 Office: 940-626-0120 pk...@wisehealthsystems.com [https://secure-web.cisco.com/1W2I8ZnRRyP53h6sYjsyA3KJtvbAABXQ_56wZrZBYbUNfmm5-C1x3Sqw0olIUR-tu8gxIsc7gCpI5WJaJ8y_D5z6FQKe3-g3_B-GzjeHOoAzFk-K9wHim2il_295OsAPxtygC_U2rIWpMEAC7_3LPdZsT9nKOGCP1Un-3w6JeSPRNRoecSHRzbV0EfFudWlHHb-0-stMEqbkjfzHt1h5X8sQbJU1W8RTxUJbe_w3T0dyUUB8Fo8HNBkCk079A_C4A38Q8ZlR78FLt-4td2_sfXA/https%3A%2F%2Fattachments.office.net%2Fowa%2Fpkara%2540wisehealthsystem.com%2Fservice.svc%2Fs%2FGetAttachmentThumbnail%3Fid%3DAAMkAGY1MTI5ZDNjLWU3NTgtNDA1NS1hM2NhLWQxODc1MDQ0MmU5MQBGAAAG%252BU6a0gKKTaEnWb7ih%252BoQBwBaK5n5dSnaQLPfKtCbo5IBAAEJAABaK5n5dSnaQLPfKtCbo5IBAABLgFJuAAABEgAQAPGmugMa86BHnNf4tfiY61Q%253D%26thumbnailType%3D2%26token%3DeyJhbGciOiJSUzI1NiIsImtpZCI6IkQ4OThGN0RDMjk2ODQ1MDk1RUUwREZGQ0MzODBBOTM5NjUwNDNFNjQiLCJ0eXAiOiJKV1QiLCJ4NXQiOiIySmozM0Nsb1JRbGU0Tl84dzRDcE9XVUVQbVEifQ.eyJvcmlnaW4iOiJodHRwczovL291dGxvb2sub2ZmaWNlLmNvbSIsInVjIjoiM2I0MmFjODRiMTVmNGMyMjkyOTk3NThhYzcwNzE5YjYiLCJzaWduaW5fc3RhdGUiOiJbXCJkdmNfbW5nZFwiLFwiZHZjX2RtamRcIixcImlua25vd25udHdrXCIsXCJr 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.WhZkvba9Eq838pqyvXzeL70kAivoZmreu0WYNt3iqB8_1-oez56A6zLQULuJr9o9ypmQQssj9deKbLQQ2PP8s_Hw_QJfoikCDf7h_Ho_oRqJ131emfs9uJCIYbfojGn7SFR73iQr8VIuzVh8shs_DmByzi2fjKxwuQIRW6EzJE-eearAUxk2oDqfeWdKyFCj-BkXeZ8FPUglxXKU-bZU0rhAryXjojYfxd7AzEU3jJuuEU5lF 1Ltpl5EfLqQLlvkwHgYIChWaWisXX5QFhJ1mM2TLUWJHiJQ-wjE5QCAtz1b99kVtgXRohYA_rIJCSW8sb853mxM74YxwohVQoMfew%26X-OWA-CANARY%3DRiiZcTdBCE2TzgqeELpeDjALI_oGFdsYiCPzjgXJu5RsBG9ZQwnwt2G0DkaG_9YPlYKQYM3yp5g.%26owa%3Doutlook.office.com%26scriptVer%3D20230210007.07%26animation%3Dtrue CONFIDENTIALITY NOTICE: This email is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521 and is legally privileged. This communication may also contain material protected and governed by the Health insurance Portability and Accountability Act of 1996 (HIPAA). This e-mail is only for the personal and confidential use of the individuals to which it is addressed and contains confidential information. If you are not the intended recipient, you are notified that you have received this document in error, and that any reading, distributing, copying or disclosure is unauthorized. If you have received this communication in error, please notify the sender immediately by the telephone number above and destroy the message ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://secure-web.cisco.com/1RC2m7IhavI1TjTTDJ7Orzxm4-5w2OR17lVwoEjed-uqwNqSdXCxizFUn3LF34svAgEDNZyjYSD0gbngtEmXHIqvvrIRswcpCCcZoPDf63NS6mCa-YHL28ZBkeferXboiQm7R8sx6mYwKhV7OTUohcCNZEVavDLOyjOd5xR1BREzqDQ0-2UsgiVez70BSGdxBBDjRtpSSCaJQSL_zXcVqfP5xPoAeLZchpUiuf4p6hX21u6pjsBNXspCcNijuE19b2Zw9vvPmKqcrOXp5nG0NHMdCkh82rP5HiUs1D9JjYcKCuacc1dSrFRD2G0xihPW12oANv5epATp5qdrlnzd3h7w4ea6rTlSJkS9NlOmymJA3FH4vH9pUMGXskt-eHYx66cCl4HcQNrw12sk1s1d22UKqq2o-mzaxrPXw8fZHSCjgT-dN6k02Q-qLe5c3REjE/http%3A%2F%2Flists.utso
[Histonet] Special Stainers
So I am working for a brand new lab that has been open since September. We currently perform all our specials by hand (roughly 7 stains; 3 silvers) and our numbers are starting to shoot up big time on them. Any recommendations on special stainers and kits that go with them? We are a Sakura flag ship lab but we are open to other equipment if the cost is good and the results are great. Phillip Kara BS, HTL(ASCP) : Lead Histology Tech Wise Pathology LLC 609 Medical Center Dr. Decatur TX. 76234 Office: 940-626-0120 pk...@wisehealthsystems.com [https://attachments.office.net/owa/pkara%40wisehealthsystem.com/service.svc/s/GetAttachmentThumbnail?id=AAMkAGY1MTI5ZDNjLWU3NTgtNDA1NS1hM2NhLWQxODc1MDQ0MmU5MQBGAAAG%2BU6a0gKKTaEnWb7ih%2BoQBwBaK5n5dSnaQLPfKtCbo5IBAAEJAABaK5n5dSnaQLPfKtCbo5IBAABLgFJuAAABEgAQAPGmugMa86BHnNf4tfiY61Q%3D&thumbnailType=2&token=eyJhbGciOiJSUzI1NiIsImtpZCI6IkQ4OThGN0RDMjk2ODQ1MDk1RUUwREZGQ0MzODBBOTM5NjUwNDNFNjQiLCJ0eXAiOiJKV1QiLCJ4NXQiOiIySmozM0Nsb1JRbGU0Tl84dzRDcE9XVUVQbVEifQ.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 ixcInByaW1hcnlzaWRcIjpcIlMtMS01LTIxLTM5MjgzMzIyMDktMzgyNDU3MTAyMy0zMDU1OTc1OTk1LTEyNTY5MzIxXCJ9IiwibmJmIjoxNjc3MDY3MTAzLCJleHAiOjE2NzcwNjc3MDMsImlzcyI6IjAwMDAwMDAyLTAwMDAtMGZmMS1jZTAwLTAwMDAwMDAwMDAwMEA0MGM5NTkxYS05M2FjLTQwNTUtOWU3NS1lNjdjYTNlZDRkYjQiLCJhdWQiOiIwMDAwMDAwMi0wMDAwLTBmZjEtY2UwMC0wMDAwMDAwMDAwMDAvYXR0YWNobWVudHMub2ZmaWNlLm5ldEA0MGM5NTkxYS05M2FjLTQwNTUtOWU3NS1lNjdjYTNlZDRkYjQiLCJoYXBwIjoib3dhIn0.WhZkvba9Eq838pqyvXzeL70kAivoZmreu0WYNt3iqB8_1-oez56A6zLQULuJr9o9ypmQQssj9deKbLQQ2PP8s_Hw_QJfoikCDf7h_Ho_oRqJ131emfs9uJCIYbfojGn7SFR73iQr8VIuzVh8shs_DmByzi2fjKxwuQIRW6EzJE-eearAUxk2oDqfeWdKyFCj-BkXeZ8FPUglxXKU-bZU0rhAryXjojYfxd7AzEU3jJuuEU5lF1Ltpl5EfLqQLlvkwHgYIChWaWisXX5QFhJ1mM2TLUWJHiJQ-wjE5QCAtz1b99kVtgXRohYA_rIJCSW8sb853mxM74YxwohVQoMfew&X-OWA-CANARY=RiiZcTdBCE2TzgqeELpeDjALI_oGFdsYiCPzjgXJu5RsBG9ZQwnwt2G0DkaG_9YPlYKQYM3yp5g.&owa=outlook.office.com&scriptVer=20230210007.07&animation=true] CONFIDENTIALITY NOTICE: This email is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521 and is legally privileged. This communication may also contain material protected and governed by the Health insurance Portability and Accountability Act of 1996 (HIPAA). This e-mail is only for the personal and confidential use of the individuals to which it is addressed and contains confidential information. If you are not the intended recipient, you are notified that you have received this document in error, and that any reading, distributing, copying or disclosure is unauthorized. If you have received this communication in error, please notify the sender immediately by the telephone number above and destroy the message ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] UNSUBSCRIBE
Unsubscribe me -Original Message- From: Naira Margaryan via Histonet Sent: Wednesday, February 22, 2023 7:16 AM To: Charles Riley Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] research questions Hi Charles, I always ran serial sections for negative control - so, yes, the same species. Good luck, Naira On Tue, Feb 21, 2023 at 2:58 PM Charles Riley via Histonet < histonet@lists.utsouthwestern.edu> wrote: > When performing IHC's for research projects is it recommended to use > the same species for control tissues or will any tissues that the > antibody has been validated for by the company be acceptable? > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] research questions
Hi Charles, I always ran serial sections for negative control - so, yes, the same species. Good luck, Naira On Tue, Feb 21, 2023 at 2:58 PM Charles Riley via Histonet < histonet@lists.utsouthwestern.edu> wrote: > When performing IHC's for research projects is it recommended to use the > same species for control tissues or will any tissues that the antibody has > been validated for by the company be acceptable? > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
