Alcian green was a mixture of two cationic "Ingrain" dyes: one of ICI's alcian
blues (a copper phthalocyanine) and their alcian yellow (CI 12840, a monoazo
dye). ICI stopped making these dyes, which were a commercial flop, in the early
1970s. It's extremely unlikely that anything now sold as "alcian green" will be
the genuine article. The original "Alcian blue" dyes were unstable even as
powders.
The staining procedure published by HD Attwood in J. Path. Bact. 76:211 (1958)
has to be seen as an oddity from the days when carbohydrate histochemistry was
still in its infancy. Any textbook published since the 1970s will provide very
simple and rational staining techniques for showing cartilage matrix
(basophilic) and keratin (acidophilic) in contrasting colours.
John Kiernan
London, Canada
= = =
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Alcian Green Dye for Attwood stain
Hi All,
I could use some assistance. I'm gotten a request to do an Attwood stain for
squames and cartilage and I'm having a difficult time finding the Alcian Green
2GX dye powder or even a kit if one exists. Another question is the protocol
that I've seen from Histonet archives states that you dilute the phloxine in
cellosolve. I would just like to ask if anyone has another version of the
protocol with any updates to the protocol?
Thanks in advance.
Debbie Siena
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From: Debra Siena via Histonet
Sent: April 5, 2023 2:47 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Alcian Green Dye for Attwood stain
Hi All,
I could use some assistance. I'm gotten a request to do an Attwood stain for
squames and cartilage and I'm having a difficult time finding the Alcian Green
2GX dye powder or even a kit if one exists. Another question is the protocol
that I've seen from Histonet archives states that you dilute the phloxine in
cellosolve. I would just like to ask if anyone has another version of the
protocol with any updates to the protocol?
Thanks in advance.
Debbie Siena
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