Re: [Histonet] Alcian Green Dye for Attwood stain

[Debra Siena via Histonet]

Hi John,

Thank you very much. I was hoping that you would also weigh in on this.

Appreciate all your wisdom and willingness to share with others.

Have a great holiday weekend!
Debbie
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From: John Kiernan via Histonet 
Sent: Thursday, April 6, 2023 12:22 AM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: Re: [Histonet] Alcian Green Dye for Attwood stain


*** Externally sourced email message ***


Alcian green was a mixture of two cationic "Ingrain" dyes: one of ICI's alcian 
blues (a copper phthalocyanine) and their alcian yellow (CI 12840, a monoazo 
dye). ICI stopped making these dyes, which were a commercial flop, in the early 
1970s. It's extremely unlikely that anything now sold as "alcian green" will be 
the genuine article. The original "Alcian blue" dyes were unstable even as 
powders.

The staining procedure published by HD Attwood in J. Path. Bact. 76:211 (1958) 
has to be seen as an oddity from the days when carbohydrate histochemistry was 
still in its infancy. Any textbook published since the 1970s will provide very 
simple and rational staining techniques for showing cartilage matrix 
(basophilic) and keratin (acidophilic) in contrasting colours.

John Kiernan
London, Canada
= = =

To: 'histonet@lists.utsouthwestern.edu' 

Subject: [Histonet] Alcian Green Dye for Attwood stain

Hi All,

I could use some assistance.  I'm gotten a request to do an Attwood stain for 
squames and cartilage and I'm having a difficult time finding the Alcian Green 
2GX dye powder or even a kit if one exists.  Another question is the protocol 
that I've seen from Histonet archives states that you dilute the phloxine in 
cellosolve.  I would just like to ask if anyone has another version of the 
protocol with any updates to the protocol?

Thanks in advance.

Debbie Siena
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From: Debra Siena via Histonet 
Sent: April 5, 2023 2:47 PM
To: 'histonet@lists.utsouthwestern.edu' 
Subject: [Histonet] Alcian Green Dye for Attwood stain

Hi All,

I could use some assistance.  I'm gotten a request to do an Attwood stain for 
squames and cartilage and I'm having a difficult time finding the Alcian Green 
2GX dye powder or even a kit if one exists.  Another question is the protocol 
that I've seen from Histonet archives states that you dilute the phloxine in 
cellosolve.  I would just like to ask if anyone has another version of the 
protocol with any updates to the protocol?

Thanks in advance.

Debbie Siena





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[Histonet] PERMANENT Histotech Opportunity – Hicksville area of New York

[Shelli DeFazio via Histonet]

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