[Histonet] A Letter To My Histopeeps. I Can Keep A Secret. Can You?

[relia1--- via Histonet]

Hello Histopeeps!

I hope you are having a great day.

Did you know you might not even see the best opportunities?

What I am seeing is about 50% of the time 

The "PLUM" Jobs Don't Get Posted!

*   It's Networking! 
*   Word of Mouth!
*   Working with Recruiters!

Ask Yourself-

Which option is your safest bet for a confidential search?

 A Recruiter! 

The majority of the leadership opportunities that I work on

are NEVER advertised!

These are CONFIDENTIAL Searches!

Confidential Because:

*   It's A New Facility
*   No One On Staff Can do the job
*   A Reorganization

Opportunities Like:

*   Management - Manager, Supervisor, Lead Tech
*   Vendor Opportunities - Leadership, Sales, Support
*   Quality Assurance/Quality Control
*   Education

When I Get One Of These Positions, I Go To The People Who Have Told Me What
THEIR Next Opportunity Looks Like!

After I speak with them I put it in our Histopeep Career Bulletin.

Want First DIBS?

Help ME, Help YOU!

Shoot me a quick email OR schedule a call and Tell Me YOUR Next Opportunity
NEEDS to Look Like!

Have a great day and Thanks for Taking the Time!

Thanks-Pam

Right Time, Right Place, Right Move with RELIA!

Providing excellent service exclusively to the Histology Community!

 

Thank You!

Pam M. Barker

Pam Barker

President/Senior Recruiting Specialist-Histology

RELIA Solutions

Specialists in Allied Healthcare Recruiting

5717 Red Bug Lake Road #330

Winter Springs, FL 32708-4969

Phone: (407)657-2027

Cell:   (407)353-5070

Toll free: (866)60RELIA or (866)607-3542

E-mail: rel...@earthlink.net 

 

 

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[Histonet] A Letter To My Histopeeps. I Can Keep A Secret. Can YOU?

[Pam Barker via Histonet]

Hello Histopeeps 

I hope you are having a great day. 

Did you know you might not even see the best opportunities? 

What I am seeing is about 50% of the time  

The "PLUM" Jobs Don't Get Posted! 

·    It's Networking!  

·    Word of Mouth! 

·    Working with Recruiters! 

Ask Yourself- 

Which option is your safest bet for a confidential search? 

  A Recruiter!  

The Majority Of The Opportunities I Represent 

Are NEVER Advertised! 

These are CONFIDENTIAL Searches!

Confidential Because: 

Ø It's A New Facility 

Ø No One On Staff Can do the job 

Ø A Reorganization 

Opportunities Like: 

v Management - Manager, Supervisor, Lead Tech 

v Vendor Opportunities - Leadership, Sales, Support 

v Quality Assurance/Quality Control 

v Education 

When I Get One Of These Positions, I Go To The People Who Have Told Me What 
THEIR Next Opportunity Looks Like! 

AFTER I speak with them I put it in our Histopeep Career Bulletin! 

Histopeeps Want First DIBS? 

Help ME, Help YOU! 

Shoot me a quick email OR schedule a call and Tell Me YOUR Next Opportunity 
NEEDS to Look Like! 

Have a great day and Thanks for Taking the Time! 

Thanks-Pam 

Right Time, Right Place, Right Move with RELIA! 

Providing excellent service exclusively to the Histology Community! 

Thank You! 

 Pam M. Barker 

Pam Barker 

President/Senior Recruiting Specialist-Histology 

RELIA Solutions 

Specialists in Allied Healthcare Recruiting 

5717 Red Bug Lake Road #330 

Winter Springs, FL 32708-4969 

Phone: (407)657-2027 

Cell:   (407)353-5070 

Toll free: (866)60RELIA or (866)607-3542 

E-mail: rel...@earthlink.net   

Click Here to Unsubscribe 

RELIA Solutions 

histonet@lists.utsouthwestern.edu 

5703 Red Bug Lake Road #330

Winter Springs, fl 32708
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Re: [Histonet] Ihc control tissues

[Colleen Forster via Histonet]

Charles,

I am in a research only lab as well. We create TMA's as control blocks
using the same idea that Val uses. Multiple tissues per block for all IHC
and also good for special stains. You can make nicely organized TMA's
manually very cost effectively as well. Feel free to reach out to me if
interested in more information.

Colleen Forster HT(ASCP)QIHC
cfors...@umn.edu

On Wed, May 8, 2024 at 9:56 AM Charles Riley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Awesome thank you Val. I like the multi control option. Can save money in
> the end by using less reagents
>
> On Wed, May 8, 2024 at 10:52 AM Val T  wrote:
>
> > Hey Charles-
> >
> > Our research lab prepares a “multimouse” control. We embed 10 organs on
> > the same slide and it serves as a +/- control for just about every IHC
> > marker.
> > However, if you don’t want to do that- here are some tissues that would
> > make nice controls.
> >
> > > BCL-2- spleen, lymph nodes
> > > Caspase-3- spleen, lymph nodes
> > > CD117- skin, spleen, brain, lymph nodes
> > > CD62E (e-selectin), bone marrow, colon
> > > Collagen II, cartilage
> > > Desmin, heart, skeletal muscle
> > > GFAP, brain, spinal cord
> > > HMB45, skin
> > > Ki67, spleen, bowel
> > > p53, tumors, colon
> > > s100, colon, muscle
> >
> > Brain can serve as a negative control on just about all these. CD117 will
> > have some expression in the cerebellum, but will be negative in most
> other
> > areas.
> > Heart can be a negative control for GFAP.
> > Val
> >
> > > On May 8, 2024, at 6:59 AM, Charles Riley via Histonet <
> > histonet@lists.utsouthwestern.edu> wrote:
> > >
> > > Hello all,
> > >
> > > I am trying to put together a positive and negative tissue control list
> > for
> > > the following antibodies at my research core.  I came over from a
> > clinical
> > > pathology background and we used tonsils for a lot of these antibodies.
> > > Since rats and mice don't have tonsils I am looking for something they
> do
> > > have that can be used if possible to keep the controls as similar to
> the
> > > samples as possible.
> > >
> > > BCL-2
> > > Caspase-3
> > > CD117
> > > CD62E (e-selectin)
> > > Collagen II
> > > Desmin
> > > GFAP
> > > HMB45
> > > Ki67
> > > p53
> > > s100
> > > ___
> > > Histonet mailing list
> > > Histonet@lists.utsouthwestern.edu
> > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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Re: [Histonet] Ihc control tissues

[Charles Riley via Histonet]

Awesome thank you Val. I like the multi control option. Can save money in
the end by using less reagents

On Wed, May 8, 2024 at 10:52 AM Val T  wrote:

> Hey Charles-
>
> Our research lab prepares a “multimouse” control. We embed 10 organs on
> the same slide and it serves as a +/- control for just about every IHC
> marker.
> However, if you don’t want to do that- here are some tissues that would
> make nice controls.
>
> > BCL-2- spleen, lymph nodes
> > Caspase-3- spleen, lymph nodes
> > CD117- skin, spleen, brain, lymph nodes
> > CD62E (e-selectin), bone marrow, colon
> > Collagen II, cartilage
> > Desmin, heart, skeletal muscle
> > GFAP, brain, spinal cord
> > HMB45, skin
> > Ki67, spleen, bowel
> > p53, tumors, colon
> > s100, colon, muscle
>
> Brain can serve as a negative control on just about all these. CD117 will
> have some expression in the cerebellum, but will be negative in most other
> areas.
> Heart can be a negative control for GFAP.
> Val
>
> > On May 8, 2024, at 6:59 AM, Charles Riley via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > Hello all,
> >
> > I am trying to put together a positive and negative tissue control list
> for
> > the following antibodies at my research core.  I came over from a
> clinical
> > pathology background and we used tonsils for a lot of these antibodies.
> > Since rats and mice don't have tonsils I am looking for something they do
> > have that can be used if possible to keep the controls as similar to the
> > samples as possible.
> >
> > BCL-2
> > Caspase-3
> > CD117
> > CD62E (e-selectin)
> > Collagen II
> > Desmin
> > GFAP
> > HMB45
> > Ki67
> > p53
> > s100
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Re: [Histonet] Ihc control tissues

[Val T via Histonet]

Hey Charles- 

Our research lab prepares a “multimouse” control. We embed 10 organs on the 
same slide and it serves as a +/- control for just about every IHC marker.
However, if you don’t want to do that- here are some tissues that would make 
nice controls.

> BCL-2- spleen, lymph nodes
> Caspase-3- spleen, lymph nodes
> CD117- skin, spleen, brain, lymph nodes
> CD62E (e-selectin), bone marrow, colon
> Collagen II, cartilage
> Desmin, heart, skeletal muscle
> GFAP, brain, spinal cord
> HMB45, skin
> Ki67, spleen, bowel
> p53, tumors, colon
> s100, colon, muscle

Brain can serve as a negative control on just about all these. CD117 will have 
some expression in the cerebellum, but will be negative in most other areas.
Heart can be a negative control for GFAP.
Val

> On May 8, 2024, at 6:59 AM, Charles Riley via Histonet 
>  wrote:
> 
> Hello all,
> 
> I am trying to put together a positive and negative tissue control list for
> the following antibodies at my research core.  I came over from a clinical
> pathology background and we used tonsils for a lot of these antibodies.
> Since rats and mice don't have tonsils I am looking for something they do
> have that can be used if possible to keep the controls as similar to the
> samples as possible.
> 
> BCL-2
> Caspase-3
> CD117
> CD62E (e-selectin)
> Collagen II
> Desmin
> GFAP
> HMB45
> Ki67
> p53
> s100
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] Ihc control tissues

[Charles Riley via Histonet]

Hello all,

I am trying to put together a positive and negative tissue control list for
the following antibodies at my research core.  I came over from a clinical
pathology background and we used tonsils for a lot of these antibodies.
Since rats and mice don't have tonsils I am looking for something they do
have that can be used if possible to keep the controls as similar to the
samples as possible.

BCL-2
Caspase-3
CD117
CD62E (e-selectin)
Collagen II
Desmin
GFAP
HMB45
Ki67
p53
s100
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