[Histonet] Validation form for Sakura Tape Coverslipper

[Eileen Akemi Allison via Histonet]

Hello out there HistoPeeps:

I am reaching out to any of you who would be gracious enough to share your 
Validation Form for the Sakura Tape Coverslipper.  I do not have one in my 
files and would be in debt to you for your kindness.  We are getting a 
refurbished tape coverslipper in the next few days and I will need the 
Validation Form sooner than later.  I know Sakura now has on their website 
“Sakura University” which explains all the operations for their equipment, but 
I need a Validation form which they don’t have.

Thank you in advance for your assistance, and have an awesome day!

Best regards,
Akemi

Akemi Allison-Tacha BS, HT/HTL (ASCP)
Pathology Laboratory Manager at Golden State Dermatology
370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598
(925) 278-7592 Ext: 10079
Mobil:: (408) 335-9994
Email: aalli...@gsdermca.com 
Website: www.goldenstatedermatology.com 
 

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[Histonet] Oven Temps and baking times used for IHC's

[Eileen Akemi Allison via Histonet]

Good morning Histo Peeps!

I am taking a poll on what various labs are using for temperature and times for 
IHC’s slides which are baked in the oven.  It has been my experience while 
working at Biocare Medical, we would 1st wick excess water off the slides, then 
tap the rack of slides on paper; then subsequently place the rack of slides in 
a 36 degree oven for 30 minutes, then up the temperature to 60-62 degrees for 
an additional time of 30-60 minutes.  Sorry, my brain cells can’t remember 
which length of time in 60 degrees, I left Biocare Medical in 2006.  My ex, 
David Tacha, Ph.D, HT/HTL gave several workshops on this subject 
internationally.

The UCSF Surgical Pathology lab 1st wicks excess water off, then bakes their 
IHC slides at 62 degrees for 2 hours.  I have consulted w/ some labs who bake 
their IHC slides at 76 degrees for 20 minutes.  It has been my experience that 
higher than 62 degree temps will make the Ab lose their antigenicity.  CK Ab’s 
love a higher temp but most other Ab’s do not.

Any thoughts, information, and papers on this subject would be greatly 
appreciated.  Thank you and have a wonderful upcoming weekend!

Best regards,
Akemi

Akemi Allison-Tacha BS, HT/HTL (ASCP)
Pathology Laboratory Manager at Golden State Dermatology
370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598
Cell: (408) 335-9994
Email: aalli...@gsdermca.com 
Website: www.goldenstatedermatology.com 

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[Histonet] Bill DeSalvo and ASSIST Tracking

[Eileen Akemi Allison via Histonet]

Hi Histo Land:

Happy TGIF!  I have a quick question; I am trying to get a hold of Bill 
DeSalvo.  I wanted to ask him about interfacing ASSIST Tracking Software with 
our IntelliPath LIS.  I called his old cell number I had from over 15 years 
ago, but I think it isn’t any good any more.  Anybody want to share his current 
contact information?  Not sure if he is working at Quest Sonora anymore, or he 
retired. 

If any of you in Histoland  are using these systems and want to chime in, 
please do.  Have a great weekend!


Best regards,
Akemi

Akemi Allison-Tacha BS, HT/HTL (ASCP)
Laboratory Manager at Golden State Dermatology
370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598
Cell: (408) 335-9994
Email: aalli...@gsdermca.com 
Website: www.goldenstatedermatology.com 


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[Histonet] BAT-1 Ab Validation

[Eileen Akemi Allison via Histonet]

Hi All my Histo Peeps in the Derm Arena: 

Do any of you who have Validated the BAT-1 Ab on a Leica want to share your 
Validation Protocol?  I sure would appreciate it.  Email me and we can talk. 


Best,

Akemi Allison-Tacha BS, HT/HTL (ASCP)
Lab Manager at Golden State Dermatology
Address: 370 N Wiget Lane
Cell: (408) 335-9994
Email: aalli...@gsdermca.com 
Website: goldenstatedermatology.com 


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[Histonet] John Caggiano’s passing

[Akemi Allison via Histonet]

Dear HistoPeeps:
I have some sad news to share with the histology/pathology community. Joe 
Caggiano informed me Monday that his brother John passed away this last Sunday 
night.  John Caggiano, founder, President and CEO of Poly Scientific R & D 
Corp, died quietly in his sleep after a long illness. John and Poly Scientific 
were early and longtime supporters of NSH, (National Society for 
Histotechnologists). He was always supportive, generous, and it was a pleasure 
to spend time with him. John was a great friend and he will be greatly missed. 
RIP John.

Regards,
Akemi Allison-Tacha, BS, HT/HTL (ASCP)



Sent from my iPhone
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Re: [Histonet] Faded H&E tissue section

[Akemi Allison via Histonet]

Hi Tony:
I actually gave that tip to Lee back in 1979 when he came to OHSU to give a 
seminar to the Oregon Histology Society.  I found out that when I was doing GMA 
and the pathologists didn’t like Gills Hematoxylin. They loved my PAS on GMA so 
I tried using 1% Periodic Acid before using Harris Hematoxylin for H&E’s on GMA 
and it turned out beautifully. Guess he shared my tip with the rest of our 
society. He and I became closer se friends and we shared several tips, 
including his Movat’s tips which he didn’t publish, but I shared them in 
Frieda’s 4th Ed.

Best,
Akemi Allison-Tacha, BS, HT/HTL (ASCP)

Sent from my iPhone

> On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet 
>  wrote:
> 
> Thanks, I'll check it out.
> 
>> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood  wrote:
>> 
>> You could treat the decoverslipped section in 1% periodic acid (same as
>> used in the PAS technique)  for 30 minutes prior to H&E staining. This
>> might improve the H&E staining.
>> 
>> 
>> 
>> I believe Lee Luna suggested this but for the life of me, I can’t find the
>> reference!
>> 
>> 
>> 
>> Regards,
>> 
>> 
>> 
>> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
>> 
>> Principal Scientist, the Children’s Hospital at Westmead (Retired)
>> 
>> Adjunct Fellow, School of Medicine, University of Western Sydney.
>> 
>> 
>> 
>> *From: *jayalakshmy p.s via Histonet 
>> *Sent: *Friday, 10 November 2023 3:43 AM
>> *To: *histonet@lists.utsouthwestern.edu
>> *Subject: *[Histonet] Faded H&E tissue section
>> 
>> 
>> 
>> Hello,
>> I would like to know how to effectively restain a faded H&E tissue section.
>> The color becomes dull when re stained. Somebody please advise.
>> Prof. Jayalakshmy. P S
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>> 
>> 
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Re: [Histonet] [External] Re: Coffee at the desk

[Akemi Allison via Histonet]

  15:4-Observing Standard Precautions

   Occupational Safety and Health Administration (OSHA)

   Can a health care worker drink coffee in a laboratory where blood/body
   fluids/tissue tests are performed? Why or Why not?

   No, federal law prohibits eating and drinking in any area that can be
   potentially contaminated by blood or other body fluids.

   Regards,

   Akemi Allison-Tacha, BS, HT/HTL ASCP

   President/Consultant

   Phoenix Laboratory Consulting

   Sent from my iPhone

 On Jun 2, 2023, at 12:37 PM, Bitting, Angela K. via Histonet
  wrote:

   Oh, for the days when one could tuck a 32 oz. Slurpy inconspicuously
   behind the VIP without fear of admonishment.
   -Original Message-
   From: Jay Lundgren via Histonet 
   Sent: Friday, June 2, 2023 3:34 PM
   To: Piche, Jessica 
   Cc: Histonet@lists.utsouthwestern.edu
   Subject: [External] Re: [Histonet] Coffee at the desk
   External: Be careful with links and files. If suspicious, alert the
   Information Security Office. Questions? Call 570-271-8092
   Next thing you're going to be telling me it's not OK to embed with a 1
   foot tall bunsen burner open flame and a Coke on the cold tray!
   On Fri, Jun 2, 2023 at 7:46 AM Piche, Jessica via Histonet <
   histonet@lists.utsouthwestern.edu> wrote:

 It also doesn't set a good impression for everyone else in the lab

 since they can't have a coffee at their stations. If it could be
 done,

 I wouldn't want everyone else's coffees on my desk either,  but I'd

 have to because I'd want to make it fair to everyone. If only it was
 safe for all of us

 Have a great weekend everyone!

 Jessica

 Jessica Piche, HT(ASCP)

 Waterbury Hospital Histology Laboratory Histology Team Leader

 203-573-7167

 

 From: McLaughlin, Stacy L. via Histonet

 ;

 Histonet@lists.utsouthwestern.edu
 

 Subject: Re: [Histonet] Coffee at the desk

 [EXTERNAL MSG]

 It is not allowed.  The area you want food/drink has to be
 completely

 separate- floor to ceiling walls and a door.

 Stacy McLaughlin, HT(ASCP) QLScm

 Histology Supervisor

 Cooley Dickinson Hospital

 30 Locust Street

 Northampton, MA 01060

 Office:  (413)582-2019

 Lab:  (413)582-2179

 smclaughl...@cooleydickinson.org

 -Original Message-

 From: Hannen, Valerie via Histonet
 

 Sent: Thursday, June 1, 2023 3:40 PM

 To: Histonet@lists.utsouthwestern.edu

 Subject: [Histonet] Coffee at the desk

External Email - Use Caution

 Is there any regulation/ reason why a Histology supervisor whose
 desk

 is in the department and the desk area is taped off and designated
 as

 a "clean area (  meaning no chemicals or specimens cross the taped
 off

 area) can't have a closed coffee cup at their desk??  We are having
 a

 debate in our Lab and I wanted to get a consensus.

 Thanks in Advance,

 Valerie

 Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief
 Parrish

 Medical Center

 951 N. Washington Avenue

 Titusville, Florida 32796

 P: 321-268-6333  Ext. 7506

 F: 321-268-6149

 valerie.han...@parrishmed.com

 http://secur/

 e-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr
 3g

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 bG

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Re: [Histonet] Alcian blue van gieson double stain

[Akemi Allison via Histonet]

Just curious, why not do a Movat’s Pentachome? It gives you everything you are 
looking for and more.  
Best regards,
Akemi Allison-Tacha, BS, Ht, HTL 
akemiat3...@gmail.com

Sent from my iPhone

> On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet 
>  wrote:
> 
> Hi,
> if they are wanting to stain elastic, Alcian blue, and Van Gieson, I 
> recommend doing the Verhoff elastic stain first, then the Alcian blue, and 
> then the Van Gieson.
> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N 
> Blue Lake DriveNorman, OK 73069PH 
> 405-759-3953http://www.excaliburpathology.com
> 
> A sharp knife is nothing without a sharp eye. - Klingon Proverb 
> 
>On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet 
>  wrote:  
> 
> I am working with a researcher and they saw an article where someone
> performed an Alcian Blue  and elastic van Gieson double stain.  If anyone
> has done this before can you provide your protocol.  I am trying to figure
> out the best way to perform this process to get the best staining results.
> Here are my questions/thoughts
> 
> 1. Is it better to run the Alcian blue as usual and then perform the van
> gieson stain?
> 2. Will the differentiating solution in the van gieson stain affect the
> alcian blue staining in any way?
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[Histonet] Validation of new G2 Coverslipper

[Eileen Akemi Allison via Histonet]

Good Morning Histo Peeps:

We just purchased a new Sakura G2 Coverslipper for our IHC Lab.  The engineer 
will be coming Monday to make sure all adjustments are in alignment and fine 
tune anything which might have gotten out of alignment due to shipping. 

I have never validated a new Coverslipper in any of the labs I have managed.  I 
searched the internet and was not able to find any information or instructions 
pertaining to Coverslipper Validation.  I am of course going to run several 
test slides prior to running any actual cases on it.  I will instruct my staff 
to date and provide test information on the slides to accompany the paperwork 
Sakura provides for the set-up of the equipment, but I am not sure that will be 
sufficient. 

Since we our a research facility, my IHC Director does not feel we need to 
validate our new Coverslipper to comply to GLP.  I just want to make sure for 
my own assurance.  I would greatly appreciate any guidelines for validation you 
may want to share with me.

Best Regards,
Akemi

Eileen (Akemi) Allison, BS, HT/HTL (ASCP)
IHC Manager
HistoTox Labs, Inc.
2108 55th St. Suite 120
Boulder, CO 80301
Cell: (408) 335-9994
Office: 303-633-5401
Email: ealli...@histotoxlabs.com 
www.histotoxlabs.com 
 
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[Histonet] GLP lot# documentation

[Eileen Akemi Allison via Histonet]

Hello Histo Land:

I am the new IHC manager for a research lab which works primarily with animal 
tissue and we are moving towards GLP compliance for our department. We have an 
extremely large client base which consists of Big Pharma, private research 
companies, universities, etc…  We are in the process of updating our SOW and 
SOP forms, Stain line maintenance forms, solution stainer change logs, etc…  

I have a question regarding documenting lot #’s for our hematoxylin 
counterstain protocol for IHC's.  Do any of you who are GLP compliant document 
lot numbers for your alcohols and xylenes?  Our director and QA officer would 
like to incorporate documenting lot numbers for alcohols and xylenes.  Our 
histology department who is GLP certified only documents their lot numbers for 
Hematoxylin, Eosin, Bluing solution and High Def solutions for their H&E stain 
line.  They currently do not document lot numbers for  alcohols and xylenes.   
I have primarily worked for Clinical pathology labs, who did not document lot 
numbers for alcohols or xylenes.  Please let me know if you do.

Thanks,
Akemi
 
Eileen (Akemi) Allison, BS, HT/HTL (ASCP)
Manager – IHC Department
HistoTox Labs, Inc.
2108 55th St. Suite 120
Boulder, CO 80301
Cell: 408-335-9994
www.histotoxlabs.com 
 
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[Histonet] Bx Processing run

[Eileen Akemi Allison via Histonet]

Good morning Histopeeps:

I am reaching out to you this morning to ask for your processing protocols for 
your biopsies and routines.  Our department has two 25 year old VIP Tissue 
processors.  Prior to my arrival, the department was only using one processor 
at a time and running the biopsies and routine tissues together.  They were 
also only rotating solutions once a week even though there were close to 300 
cassettes a day.

With that being said, we are now using both tissue processors and separating 
biopsies from routines and we rotate all solutions daily.  The specimens are 
put on the machines at 5:00 PM.  This is not an option.  Our biopsies finish at 
5:00 AM and the routines finish at 6:30 AM.  I would like to have our biopsies 
finish by 3:00 AM and the routines finish by 5:00 AM.  Naturally, I do not want 
to compromise the quality of our tissue samples.  I would appreciate you 
sharing your processing protocols with me which would include solutions and 
time in each station.

Thank you in advance for your information.

Akemi Allison, BS, HT/HTL (ASCP)
Histology Supervisor
UMC El Paso, TX


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[Histonet] Leica's Spectra w/ coverslipper

[Eileen Akemi Allison via Histonet]

Good morning Histopeeps:

We will be receiving our new Spectra H&E Stainer w/ cover slipper the week of 
May 4th.  This instrument was in negotiations for purchase prior to me coming 
on board.  I have worked with the older models of Leica’s H&E Stainers, but am 
unfamiliar with this one.  Could any of you who has this unit be willing to 
share your thoughts with me.  It’s been several years since I have validated a 
new H&E stainer.  Do I need to validate the instrument along with validating 
and optimizing the H&E stains?

Thank you in advance for your information.

Akemi Allison, BS, HTL/HT (ASCP)


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[Histonet] histologists assisting with transplants

[Eileen Akemi Allison via Histonet]

Good morning histoland!
Just curious, this is definitely a 1st for me.  Received a call this morning 
from my histotech who is on call this week that she was called in at 3:30 AM to 
assist one our pathologists with a liver transplant.  In all my years working 
in this field, histologists have never had anything to do with transplants.  
Have any of you out there had anything to do with transplants?

Akemi Allison, BS, HTL
Histology Supervisor
UMC El Paso, TX
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[Histonet] HistoQIP/PIP

[Eileen Akemi Allison via Histonet]

Hello Histopeeps:

Can someone tell me what year HistoQip start with NSH/CAP and PIP with CAP.

Thank you!

Akemi Allison, BS, HT/HTL


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[Histonet] Histotech openings in El Paso, TX

[Eileen Akemi Allison via Histonet]

Happy Friday Histopeeps!

Any Histotechs want to move to El Paso, TX?  El Paso has a lot to offer!  We 
have one current opening and another coming up in the next month.  We are 
losing one of our best tech’s to FL.  Her husband is a pathologist who is going 
to do his GI fellowship in Miami.  Our lab is getting a Leica Upgrade starting 
in March!  A Ceribro system, 2 new Bonds, a new H&E Stainer with Cover Slipper, 
and Cassette and Slide Printer.  Email me and I will provide all information.

Akemi Allison, BS, HT/HTL
Histology Supervisor
UMC El Paso, TX

Any Histotech's want to move to El Paso? 2 openings coming up in my lab!
Any Histotech's want to move to El Paso? 2 openings coming up in my lab!
Any Histotech's want to move to El Paso? 2 openings coming up in my lab!
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[Histonet] Tissue disposal

[Eileen Akemi Allison via Histonet]

Good morning Histopeeps! After reviewing our Policy Procedure Manual, our 
manager is questioning the bags we are using for disposing our tissues after 
diagnosis is complete.  We are separating the tissue from the formalin by 
straining the excess formalin and neutralizing it.  We then put the tissue into 
“Large Biohazard Bags” and the EVS staff picks it up and disposes it.  My 
manager is wondering if the “Biohazard Bags" are appropriate.  Should the bags 
state “Tissue” on the them?  Are there any special disposal bags available?  

While working for a GI Lab in Monterey, CA we put all our bottle and tissue in 
"Biohazard Bags" and a Hazardous Waste company picked up the waste products 
from our endoscopy department along with ours.  Any comments and suggestions 
would be greatly appreciated.

Thank you in advance,
Akemi Allison, BS, HT/HTL


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Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel

[Eileen Akemi Allison via Histonet]

Went on line to get MSDS regarding HistoGel.  Here’s the link.  
https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf 
<https://www.medline.com/media/catalog/Docs/MSDS/MSD_SDSD91968.pdf>

Happy eating from a microwave which has been used at MD Anderson for HistGel!

Best regards,
Akemi

> On Dec 12, 2019, at 5:18 AM, Kathleen S Cormier  wrote:
> 
> Can I share that we had a pathologist  (back in the day) use our lab 
> microwave to re-heat his coffee? It had Schiff’s reagent stains all over the 
> inside of it. Some people really don’t have any common sense… 
> 
> 
> Kathy Cormier
> Core Leader
> Hope Babette Tang Histology Facility
> Koch Institute for Integrative Cancer Research at MIT
> 500 Main Street, 76-182
> Cambridge, MA 02139
> 617-258-8183
> corm...@mit.edu <mailto:corm...@mit.edu> 
> https://ki.mit.edu/sbc/histology <https://ki.mit.edu/sbc/histology>
> 
> 
> 
> 
> 
> 
> 
> 
> 
>> On Dec 12, 2019, at 7:12 AM, Eileen Akemi Allison via Histonet 
>> > <mailto:histonet@lists.utsouthwestern.edu>> wrote:
>> 
>> Good morning histopeeps:
>> 
>> We recently brought on an Ophthalmology Pathologist from MD Anderson and 
>> they use histogel for orienting their thin eye specimens and need a 
>> microwave to heat it up.  We do not have a microwave in the lab for their 
>> use but I had ordered one for them.  I just found out they used the 
>> microwave in our lab lounge to heat up histonet which was opened and being 
>> stored in our gross area refrigerator.  
>> 
>> Do any of you know the CAP regulation against use of laboratory reagents 
>> being used in a microwave being used for food consumption?  I ended up 
>> removing the microwave they used and put it in the histology lab.  Our 
>> Director wants me to write a formal letter to submit to him.
>> 
>> Thank you in advance for your impute!
>> 
>> Akemi Allison, BS,  HT/HTL (ASCP)
>> 
>> 
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[Histonet] guidelines for using a microwave designated for food, but used to heat up histogel

[Eileen Akemi Allison via Histonet]

Good morning histopeeps:

We recently brought on an Ophthalmology Pathologist from MD Anderson and they 
use histogel for orienting their thin eye specimens and need a microwave to 
heat it up.  We do not have a microwave in the lab for their use but I had 
ordered one for them.  I just found out they used the microwave in our lab 
lounge to heat up histonet which was opened and being stored in our gross area 
refrigerator.  

Do any of you know the CAP regulation against use of laboratory reagents being 
used in a microwave being used for food consumption?  I ended up removing the 
microwave they used and put it in the histology lab.  Our Director wants me to 
write a formal letter to submit to him.

Thank you in advance for your impute!

Akemi Allison, BS,  HT/HTL (ASCP)


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Re: [Histonet] Questions on how to operate a ThermoFisher Lab Vision autostainer 3602D

[Eileen Akemi Allison via Histonet]

Ask Biocare or Dako.  Unbeknownst to most of the public, it is the same exact 
unit they had years ago.  Lab Vision made all 3 units. Their brand, Biocare and 
Dako.  Lab Vision just put different branding on the various units when they 
were sold.

Akemi Allison-Tacha, BS, HT/HTL (ASCP)
Former Director and Operations Manager at Histology at Biocare

> On Oct 24, 2019, at 12:49 PM, Bummer, Katherine via Histonet 
>  wrote:
> 
> Hello Histonetters,
> 
> My company has acquired a refurbished ThermoFisher Lab Vision autostainer 
> 3602D and I have questions on how to operate.  It is no longer supported by 
> ThermoFisher so I am unable to get tech support through them.  Is there 
> anyone who is familiar and available/willing to answer a few questions?
> 
> Thank you in advance.
> 
> Kate
> 
> Katherine Bummer
> Nektar Therapeutics
> 455 Mission Bay Boulevard South
> San Francisco, CA  94158
> k.bum...@nektar.com
> 415.482.5716
> 
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[Histonet] hints for cutting fatty FS

[Eileen Akemi Allison via Histonet]

A while back on histonet a few people gave some tricks to cutting fatty tissue 
on histonet.  Someone mentioned using 50% alcohol I believe.  We had some 
extremely fatty LN’s yesterday.  The pathologist cut away most of the fat, but 
the tech still had a hard time, so I got called in to help the tech.  I was 
able to get sufficient sections, but they weren’t optimal.  Could any of you 
share your secrets please. 

Thank you,
Akemi Allison, BS, HT/HTL (ASCP)
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[Histonet] Cutting fatty FS

[Eileen Akemi Allison via Histonet]

Good Morning Histoland,
A while back on histonet a few people gave some tricks to cutting fatty FS 
tissue on histonet.  Someone mentioned using 50% alcohol I believe.  We had 
some extremely fatty FS LN’s yesterday.  The pathologist cut away most of the 
fat, but the tech still had a hard time, so I got called in to help the tech.  
I was able to get sufficient sections, but they weren’t optimal.  Could any of 
you share your secrets please. 

Thank you,
Akemi Allison, BS, HT/HTL (ASCP)
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[Histonet] Histo techs assisting on BM Bx's

[Eileen Akemi Allison via Histonet]

Good Morning Histo Peeps:
Back from a great trip to NSH NOLA and hit the ground running yesterday.  I am 
faced with a department coverage issue which impacts our TAT.  I am curious how 
many facilities have their histologists assist with BM Bx’s.  I have never in 
my 50 years as a histologist assisted with BM Bx’s.  That responsibility was 
under the hematology department responsibilities.

We have 4 FT Histo tech’s and 1 PNR in our department.  Yesterday we had 2 
histologists pulled away from bench duties to assist with BM Bx’s, and then 
another histo tech was called to go to the main hospital to cut FS’s, which is 
2 blokes away.  One of the tech’s was gone for 3 hrs because the pathologist 
performing the BM was extremely slow.  That left 1 tech and myself to cover 
bench duties.  

Keep in mind this is a county hospital with limited funding and a freeze on 
staffing.

Thank you in advance for your input.
Akemi

Akemi Allison, BS, HT/HTL
Histology Supervisor
UMC El Paso, TX


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[Histonet] Disposal of contaminated gloves, paper towels etc... after grossing

[Eileen Akemi Allison via Histonet]

Good morning Histo Peeps:

I am curious as to your laboratory/hospital policies on how to dispose of 
contaminated gloves, paper towels etc… after the PA/pathologists gross a 
specimen.  

Often times we receive fresh tissue in the lab and we do not know if the 
patient has TB, Hepatitis, HIV, or any other infectious disease.  I was always 
taught that to treat these items universally as potential biohazards.  Do you 
discard these items in a Biohazard Waste Container,, or do you discard them in 
a regular trash basket?  

Thank you in advance for your feedback. 

Akemi Allison, BS, HT/HTL (ASCP)
Histology Manager
UMC El Paso, TX
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[Histonet] Plus Slides

[Eileen Akemi Allison via Histonet]

Good morning:

I just to to thank everyone who responded to my recent inquiry regarding (+) 
charged slides for IHC.  I am getting samples sent to me to test from a few 
vendors.

Thanks again!
Akemi Allison, BS, HT/HTL (ASCP)
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[Histonet] Plus Slides

[Eileen Akemi Allison via Histonet]

Good morning Histo Peeps!

I would like to get information on where you are getting your Plus Slides for 
IHC.  We are currently getting our (+) charged slides from Cardinal Health and 
they have little or no charge.  We currently have 2 Ventana IHC Instruments, as 
well a Special Stainer.  We are having tissues falling partially and completely 
off even on the SS Stainer. Also, I realize Ventana will not back the IHC 
results unless the end user uses a particular brand of (+) charged slides.  

Years ago when I worked at Biocare Medical I used to QC our Kling-On Slides 
before selling them by checking to see if the sections indeed stuck to the 
slides without any lifting on each lot.  I realize that positive charged slides 
have an expiration life, and these slides are newer lot numbers within their 
expiration.  

Thank you in advance for your assistance!

Akemi Allison, BS, HT/HTL (ASCP)
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[Histonet] recomendations on new cryostats

[Eileen Akemi Allison via Histonet]

Good Morning Histo Peeps!

Our facility is looking to purchase 2 new cryostats.  Can any of my 
pathology/histology colleagues recommend which you prefer.  Pros and cons of 
the Sakura and Leica cryostats would be greatly appreciated.

Thank you in advance for your input!

Warm regards,
Akemi Allison, BS HT/HTL (ASCP)
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Re: [Histonet] test

[Eileen Akemi Allison via Histonet]

I received it.  I have had the same problem lately.  I didn’t get a copy of the 
email sent.  It would be nice to know that it was received. Think they changed 
their format.

Akemi Allison

> On Nov 16, 2018, at 7:25 AM, Campbell, Tasha M. via Histonet 
>  wrote:
> 
> I keep trying to send an email for help with an issue I am having and its not 
> going through. It says the email has been sent but I am not getting the email 
> and no one has responded.  I am testing to see if this message goes through.
> 
> 
> 
> 
> Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144
> 
> 
> CONFIDENTIALITY NOTICE: This electronic mail transmission and any 
> accompanying data files is confidential and is intended exclusively for the 
> individual or entity to which it is addressed. The communication may contain 
> information that is proprietary, privileged or confidential or otherwise 
> legally exempt from disclosure. If you are not the named addressee or you 
> otherwise have received this message in error, you are not authorized to 
> read, print, copy or disseminate this message or any part of it. If you have 
> received this message in error, please notify the sender immediately by email 
> and delete all copies of this message. Receipt by anyone other than the named 
> addressee is not a waiver of any attorney-client work product or other 
> applicable privilege.
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[Histonet] Stain removal on clothing

[Eileen Akemi Allison via Histonet]

Just found this info on-line for stain removal.  I assume it originally came 
from Chuck Churukian since it’s from University of Rochester.


REMOVING STAINS FROM HANDS AND CLOTHING

Some stains can be removed form the hands by using *Erado-Sol.  Dilute Chlorox 
(or similar bleach) and Erado-Sol are useful for removing stains from white 
clothing.  Care should be exercised in removing stains from colored clothing.

To remove the following stains use the solutions indicated.

1.Alcian blue - to remove from the hands use the acid alcohol solution. 
 For cleaning glassware try using dilute Chlorox or acid alcohol.  If the stain 
resists, use them alternatively.
2.Auramine-rhodamine - acid alcohol.
3.Basic fuchsin - Erado-Sol.
4.Carmine - acid alcohol.
5.Chromic acid - concentrated aqueous sodium thiosulfate containing a 
few drops of sulfuric acid.
6.Crystal violet - Erado-Sol or Chlorox.
7.Eosin Y - saturated aqueous lithium carbonate.
8.Fast green and similar acid dyes - saturated aqueous lithium 
carbonate.
9.Hematoxylin (alum and iron) - Erado-Sol or acid alcohol.
10.  Iodine - 5% sodium thiosulfate.
11.  Methylene blue - acid alcohol, Erado-Sol or Chlorox.
12.  Nuclear fast red - acid alcohol.
13.  Picric acid - saturated aqueous lithium carbonate.
14.  Potassium permanganate - 5% oxalic acid.
15.  Schiffs solution - Erado-Sol or Chlorox.
16.  Silver deposits - Lugols iodine followed by 5% sodium thiosulfate.
17.  Sudan stains (Oil red O, Sudan black B) - acetone.
18.  Thioflavin T - acid alcohol.


*American Scientific Products
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[Histonet] Alcian Blue stain on lab coats

[Eileen Akemi Allison via Histonet]

Hello Histo Peeps:

I have two new lab coats which have been stained with Alcian Blue and can’t get 
the stains out.  I have tried oxy clean incorporated with bleach, as well as 
pre-soaking in 3% acetic acid, and American Mastertech’s stain remover to no 
avail.  Any suggestions would be greatly appreciated.

Thank you in advance,

Akemi Allison, BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 
Tele: (831) 375-3577 X117
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[Histonet] Histology position in Beautiful Montery, CA

[Eileen Akemi Allison via Histonet]

HISTOLOGY POSITION IN BEAUTIFUL MONTEREY, CA

ATTENTION HISTOLOGISTS who meet CLIA grossing regulations and are HT or HTL 
certified. We have an opening in our pathology laboratory to meet the need of 
our growing medical group.

Our lab has a formalin-free environment with new Milestone microwave hybrid 
tissue processing technology, Leica 2255 microtomes, a new Leica embedding 
center, a Leica H&E stainer, and a new Sakura cassette and slide printer.

GI experience preferred, but we will train a knowledgeable and willing 
candidate. We insist on quality embedding and microtomy skills. Duties to 
include (but not limited to): grossing, embedding, microtomy, limited manual 
special stains, manual coverslipping, general histology and histology related 
clerical duties, accessioning, and data entry into our AP Easy LIS System.

The right candidate is reliable, self-motivated, articulate, and well versed 
with anatomy identifications. Must be adept with Microsoft, Excel, and possess 
excellent keyboarding skills.

The lab is located in a private practice Gastroenterology medical group in 
beautiful Monterey, CA., near the world-famous Pebble Beach and 
Carmel-by-the-Sea. This job is full-time days, Monday through Friday with NO 
WEEKEND’s and no travel. We offer a benefits package including medical, dental 
and vision insurance, paid holidays, vacation, sick accrual and a profit 
sharing 401K plan. Salary is negotiable depending on experience.

Job Type: Full-time  Please forward your cover letter and Resume to:

Akemi Allison, BS, HT (ASCP) HTL
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
Work: (831) 375-3577  X117
Email: aalli...@montereygi.com
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[Histonet] Histology Opening in Beautiful Monterey, CA

[Eileen Akemi Allison via Histonet]

ATTENTION HISTOLOGISTS! Do you know of anyone who is looking for a job? We have 
an opening in our pathology laboratory located in a private practice 
Gastroenterology Medical Group in beautiful Monterey, CA.  It is a great 
opportunity for someone who is HT or HTL certified. The candidate must also 
meet CLIA grossing regulations. 

Our lab has a formalin-free environment with new Milestone microwave hybrid 
tissue processing technology, Leica 2255 Microtome's, New Leica Embedding 
Center and Leica H&E Stainer.

GI experience helpful, but we will train a knowledgeable and willing candidate. 
We insist on quality embedding and cutting skills. Duties to include (but not 
limited to): accessioning, data entry into our AP Easy LIS System, grossing, 
embedding, microtomy, limited manual special stains, manual coverslipping, 
general histology and histology related clerical duties. The right candidate is 
reliable, self-motivated, articulate and well versed with anatomy 
identifications. Must be adept with Microsoft, Excel, and having excellent 
typing skills.

Full Time Days, Monday through Friday with NO WEEKEND’S!  We offer A benefits 
package including medical, dental and vision insurance, paid holidays, 
vacation, sick accrual and a profit sharing 401K plan.  Salary is negotiable 
depending on experience. If you are interested, or if you know of someone, 
contact me and I will give you more details.

Akemi Allison, BS, HT/HTL (ASCP) 
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W.Email: aalli...@montereygi.com
H. Email: akemiat3...@gmail.com
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[Histonet] Histology Opening in Beautiful Monterey, CA

[Eileen Akemi Allison via Histonet]

ATTENTION HISTOLOGISTS! Do you know of anyone who is looking for a job? We have 
an opening in our pathology laboratory located in a private practice 
Gastroenterology Medical Group in beautiful Monterey, CA.  It is a great 
opportunity for someone who is HT or HTL certified. The candidate must also 
meet CLIA grossing regulations. 

Our lab has a formalin-free environment with new Milestone microwave hybrid 
tissue processing technology, Leica 2255 Microtome's, New Leica Embedding 
Center and Leica H&E Stainer.

GI experience helpful, but we will train a knowledgeable and willing candidate. 
We insist on quality embedding and cutting skills. Duties to include (but not 
limited to): accessioning, data entry into our AP Easy LIS System, grossing, 
embedding, microtomy, limited manual special stains, manual coverslipping, 
general histology and histology related clerical duties. The right candidate is 
reliable, self-motivated, articulate and well versed with anatomy 
identifications. Must be adept with Microsoft, Excel, and having excellent 
typing skills.

Full Time Days, Monday through Friday with NO WEEKEND’S!  We offer A benefits 
package including medical, dental and vision insurance, paid holidays, 
vacation, sick accrual and a profit sharing 401K plan.  Salary is negotiable 
depending on experience. If you are interested, or if you know of someone, 
contact me and I will give you more details.

Akemi Allison, BS, HT/HTL (ASCP) 
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W.Email: aalli...@montereygi.com 
H. Email: akemiat3...@gmail.com 
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Re: [Histonet] Supervising a histologist

[Eileen Akemi Allison via Histonet]

We are CLIA/CMS certified only.  My lab assistant only has a high school degree 
and never had any background in any sciences. She knows nothing about 
trouble-shooting, nor theory and practice. She has worked primarily in clerical 
roles for our GI practice for 13 years. In the past 1.5 years, I trained her to 
accession, process, embed, cut, stain, and coverslip. We do not do IHC here. 
Our CEO/GI, MD said she could oversee our new HTL during my absence.

Akemi Allison

> On Nov 1, 2017, at 5:43 AM, Joseph Saby  wrote:
> 
> Eileen-
> 
> I think it would depend on what specific procedures both of them are signed 
> off on.
> And it would be on specific procedures.
> Or things are very strange in that area of Histoland.
> 
> Joe Saby
> 
> 
> From: Eileen Akemi Allison via Histonet 
> To: Histonet  
> Sent: Wednesday, November 1, 2017 8:20 AM
> Subject: [Histonet] Supervising a histologist
> 
> Hello Histoland:
> 
> This may sound like a strange question, but I have my reasons.  Has anyone 
> run into a situation where a OJT histology assistant supervises an HTL when 
> the histology manager is absent?  Is it even legal?  Any feedback would be 
> greatly appreciated.
> 
> 
> Akemi Allison BS, HT/HTL (ASCP)
> Pathology Manager
> Monterey Bay GI Consultants Laboratory
> 23 Upper Ragsdale Drive, Suite 200
> Monterey, CA 93940
> 
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> 
> 

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[Histonet] Supervising a histologist

[Eileen Akemi Allison via Histonet]

Hello Histoland:

This may sound like a strange question, but I have my reasons.  Has anyone run 
into a situation where a OJT histology assistant supervises an HTL when the 
histology manager is absent?  Is it even legal?  Any feedback would be greatly 
appreciated.


Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940

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Re: [Histonet] CAP question ANP.12500

[Eileen Akemi Allison via Histonet]

We keep ours for one week after final report. There have been past issues as to 
tissues  submitted from our endoscopy center not matching up to our gross 
exams. Gastric tissue included with esophageal tissue, I account for every 
MINUTE fragment, so there were absolutely floaters from somewhere!. We account 
for everything when I gross the tissue!  I have a embedding log sheet which 
accounts for every piece submitted, as well as who embeds it, cuts, it, etc.. 
as well as any comments which just don’t match up to the gross.

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 
Tele: (831) 375-3577 X117
Cell: (408) 335-9994

> On Sep 15, 2017, at 8:46 AM, Vickroy, James via Histonet 
>  wrote:
> 
> Wondered if something has changed on the question pertaining to retention.   
> We only do biopsies and keep the containers they come in for two days.   We 
> have done this forever.   Going through the new checklist I notice under wet 
> tissue it has (stock bottle).   Before I interpret this incorrectly, a stock 
> bottle to me meant left over fixed tissue not taken for sections.  Last week 
> I got an email saying this other institution was keeping the empty containers 
> for two weeks after final report.
> 
> Obviously, it is clear if we have left over tissue we hold it for the 
> specified time period but does this also mean we should be holding the empty 
> biopsy container for two weeks plus also?
> 
> I hope I am overthinking this since we don't have room for them.
> 
> Your thoughts?
> 
> Jim
> 
> Jim Vickroy
> Histology Manager
> Springfield Clinic, Main Campus, East Building
> 1025 South 6th Street
> Springfield, Illinois  62703
> Office:  217-528-7541, Ext. 15121
> Email:  jvick...@springfieldclinic.com
> 
> 
> 
> This electronic message contains information from Springfield Clinic, LLP 
> that may be confidential, privileged, and/or sensitive. This information is 
> intended for the use of the individual(s) or entity(ies) named above. If you 
> are not the intended recipient, be aware that disclosure, copying, 
> distribution, or action taken on the contents of this information is strictly 
> prohibited. If you have received this electronic message in error, please 
> notify the sender immediately, by electronic mail, so that arrangements may 
> be made for the retrieval of this electronic message. Thank you.
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Re: [Histonet] RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017

[Eileen Akemi Allison via Histonet]

Tim: I know full well about that facility and have known about their activity 
for several years!  I am at a point in my life I really could care less about 
any consequences! I am Too old to care about being PC when it comes to the care 
and nurturing of histologists! I am 67 and all about patient care and what we 
do to do our best to produce the best quality we can!  What can anyone do to me 
at this point in my life???

Akemi Allison-Tacha BS, HT/HTL ASCP (HT 1968/HTL 1982)

> On Aug 1, 2017, at 4:09 PM, Morken, Timothy  wrote:
> 
> OOO, better watch it, that could get some people mad!!
> 
> Tim Morken
> Pathology Site Manager, Parnassus 
> Supervisor, Electron Microscopy/Neuromuscular Special Studies
> Department of Pathology
> UC San Francisco Medical Center
> 
> -Original Message-----
> From: Eileen Akemi Allison via Histonet 
> [mailto:histonet@lists.utsouthwestern.edu] 
> Sent: Tuesday, August 01, 2017 3:24 PM
> To: Pam Barker; Histonet
> Subject: [Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you 
> vacation 8-1-2017
> 
> Pam: No one in their right mind would work in that lab in Modesto!  They have 
> a horrible track record for numerous years! Run and don’t even think about 
> applying! 
> Akemi
> 
>> Begin forwarded message:
>> 
>> From: "Pam Barker" 
>> Subject: RELIA Histology Careers Bulletin - Live where you vacation 
>> 8-1-2017
>> Date: August 1, 2017 at 1:26:51 PM PDT
>> To: "Akemi Allison" 
>> 
>> Hi Akemi!
>> What are you doing on your summer vacation?  
>> Been anyplace you would like to move to?   
>> Whatever you want to do, wherever you want to be, I can help!
>> 
>> There is still time this summer to make that move.  
>> I have opportunities in all kinds of labs in all areas of the country. 
>> Your next position is just a phone call or an e-mail away.
>> 
>> All of the opportunities that I represent are permanent full time positions 
>> with companies that offer excellent salaries, benefits and relocation 
>> assistance.  
>> 
>> Here are some of the opportunities that I am most excited about:
>> 
>> HISTOLOGY SUPERVISORS AND MANAGERS:
>> Histology Lab Manager – Atlanta, GA
>> Histology Supervisor – Atlanta, GA
>> Histology Supervisor – Boston, MA
>> Histology Supervisor – Irvine, CA
>> Histology Supervisor – Modesto, CA
>> Lead Histotech – Los Angeles – BRAND NEW LAB Study Director – Fort 
>> Collins CO HISTOTECHNICIANS/HISTOTECHNOLOGISTS
>> CA – San Diego – brand new lab CLIA qualified to gross CA – Irvine – 
>> learn IHC CA – Los Angeles – Lead Histotech Brand New Lab!
>> TX – Nacogdoches day shift hospital environment CO – Glenwood day 
>> shift hospital environment TN – Chattanooga – CLIA qualified to gross 
>> 3rd shift generous shift diff.
>> MA – Greater Boston Area  - 2nd and 3rd shift generous shift diff.
>> 
>> If you happen to know anyone I am also looking for a cytotech in 
>> Clearwater, FL
>> 
>> If you or anyone you know are interested in hearing more about any of these 
>> opportunities or have another type of position or area in mind and would 
>> like some help in your job search please let me know.  Just shoot me an 
>> e-mail at rel...@earthlink.net <mailto:rel...@earthlink.net> or give me a 
>> call toll free at 866-607-3542 or on my cell at 407-353-5070.  
>> 
>> Thanks-Pam
>> 
>> Right Place, Right Time, Right Move with RELIA!
>> 
>> Thank You!
>> Pam M. Barker
>> 
>> Pam Barker
>> President/Senior Recruiting Specialist-Histology RELIA Solutions 
>> Specialists in Allied Healthcare Recruiting
>> 5703 Red Bug Lake Road #330
>> Winter Springs, FL 32708-4969
>> Phone: (407)657-2027
>> Cell: (407)353-5070
>> FAX: (407)678-2788
>> E-mail: rel...@earthlink.net <mailto:rel...@earthlink.net> 
>> https://www.facebook.com/RELIASolutionsforhistologyprofessionals 
>> <https://www.facebook.com/RELIASolutionsforhistologyprofessionals>
>> www.facebook.com 
>> <http://www.facebook.com/PamBarkerRELIA>/PamBarkerRELIA
>> www.linkedin.com/in/reliasolutions 
>> <http://www.linkedin.com/in/reliasolutions>
>> www.twitter.com/pamatrelia <http://www.twitter.com/pamatrelia>
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[Histonet] Fwd: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017

[Eileen Akemi Allison via Histonet]

Pam: No one in their right mind would work in that lab in Modesto!  They have a 
horrible track record for numerous years! Run and don’t even think about 
applying! 
Akemi

> Begin forwarded message:
> 
> From: "Pam Barker" 
> Subject: RELIA Histology Careers Bulletin - Live where you vacation 8-1-2017
> Date: August 1, 2017 at 1:26:51 PM PDT
> To: "Akemi Allison" 
> 
> Hi Akemi!
> What are you doing on your summer vacation?  
> Been anyplace you would like to move to?   
> Whatever you want to do, wherever you want to be, I can help!
>  
> There is still time this summer to make that move.  
> I have opportunities in all kinds of labs in all areas of the country. 
> Your next position is just a phone call or an e-mail away.
>  
> All of the opportunities that I represent are permanent full time positions 
> with companies that offer excellent salaries, benefits and relocation 
> assistance.  
>  
> Here are some of the opportunities that I am most excited about:
>  
> HISTOLOGY SUPERVISORS AND MANAGERS:
> Histology Lab Manager – Atlanta, GA
> Histology Supervisor – Atlanta, GA
> Histology Supervisor – Boston, MA
> Histology Supervisor – Irvine, CA
> Histology Supervisor – Modesto, CA
> Lead Histotech – Los Angeles – BRAND NEW LAB
> Study Director – Fort Collins CO
> HISTOTECHNICIANS/HISTOTECHNOLOGISTS
> CA – San Diego – brand new lab CLIA qualified to gross
> CA – Irvine – learn IHC
> CA – Los Angeles – Lead Histotech Brand New Lab!
> TX – Nacogdoches day shift hospital environment
> CO – Glenwood day shift hospital environment
> TN – Chattanooga – CLIA qualified to gross 3rd shift generous shift diff.
> MA – Greater Boston Area  - 2nd and 3rd shift generous shift diff.
>  
> If you happen to know anyone I am also looking for a cytotech in Clearwater, 
> FL
>  
> If you or anyone you know are interested in hearing more about any of these 
> opportunities or have another type of position or area in mind and would like 
> some help in your job search please let me know.  Just shoot me an e-mail at 
> rel...@earthlink.net  or give me a call toll 
> free at 866-607-3542 or on my cell at 407-353-5070.  
>  
> Thanks-Pam
>  
> Right Place, Right Time, Right Move with RELIA!
>  
> Thank You!
>  Pam M. Barker
>  
> Pam Barker
> President/Senior Recruiting Specialist-Histology
> RELIA Solutions
> Specialists in Allied Healthcare Recruiting
> 5703 Red Bug Lake Road #330
> Winter Springs, FL 32708-4969
> Phone: (407)657-2027
> Cell: (407)353-5070
> FAX: (407)678-2788
> E-mail: rel...@earthlink.net 
> https://www.facebook.com/RELIASolutionsforhistologyprofessionals 
> 
> www.facebook.com /PamBarkerRELIA
> www.linkedin.com/in/reliasolutions 
> www.twitter.com/pamatrelia 
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Re: [Histonet] I have a job

[Eileen Akemi Allison via Histonet]

I thought we weren’t allowed to post jobs on histonet! I tried and it was 
removed! 

Akemi Allison-Tacha

> On Mar 27, 2017, at 11:45 AM, Caryn Anderton via Histonet 
>  wrote:
> 
> Hello-
> 
> I wanted to know if it was possible to post a position that I have available? 
>  See Below
> 
> Immunohistochemistry Product Manager Communications Expert- Part Time
> 
> Summary:
> The position will focus on working with three internal groups within Biocare 
> Medical:  Marketing, Engineering and R&D. We're looking for a driven, 
> resourceful technical writing and communication expert to manage the process 
> of writing our customer-facing product documentation. You'll manage the 
> end-to-end process of drafting, writing, and releasing documentation, 
> tutorials, developer guides for:  internal and client trainings, sales 
> collateral and product manuals.
> Engineering & R&D:
> Collaborate with the Engineering team to launch a new automated IHC slide 
> staining platform. Engage with the teams to understand the instrument and how 
> it works and be able to translate technical concepts, and make them easy to 
> understand for our end users. Create and maintain reference and user guides, 
> including hardware and software documentation
> Marketing:
> Work with marketing to supply scientific knowledge and technical/medical 
> writing for product catalogs, marketing brochures and website content. Work 
> with training department to develop sales training programs.
> Requirements:
> 
> ?  Broad knowledge base in Histology , Immunohistochemistry and related 
> instrumentation
> 
> ?  Excellent writing, editing, and proofreading skills with meticulous 
> attention to detail
> 
> ?  Ability to present information and generate content appropriate market 
> segments
> 
> ?  Self-motivated, independent, able to prioritize and manage multiple 
> projects
> 
> ?  Demonstrate capacity to work collaboratively within cross-functional teams
> 
> ?  Proven and consistent track record of meeting project deadlines with 
> quality results
> 
> ?  Excellent organizational and time management skills
> 
> ?  Ability to receive creative direction and feedback
> 
> ?  Please include a cover letter and curriculum-writing sample with your 
> application.
> Biocare Medical is a leading supplier of automated immunohistochemistry (IHC) 
> instrumentation, antibodies and the full range of reagents for IHC. Biocare's 
> portfolio includes novel and established primary antibodies and FISH probes 
> for many tissue types.
> EEO/AA Employer/Veterans/Disabled/Race/Ethnicity/Gender/Age
> 
> To all recruitment agencies: Biocare does not accept agency resumes. Biocare 
> will not pay for any fees related to unsolicited resumes.
> 
> Job Location-Pacheco, California, United States
> Department-Marketing
> Position Type-Part Time
> 
> 
> 
> 
> 
> 
> Caryn M. Anderton
> Senior Director of Marketing
> Biocare Medical, LLC.
> 60 Berry Dr.
> Pacheco, CA 94553
> (925) 768-1270
> www.biocare.net
> 
> 
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Re: [Histonet] I have a job

[Eileen Akemi Allison via Histonet]

I thought we weren’t allowed to post jobs on histonet! I tried and it was 
removed! 

Akemi Allison-Tacha

> On Mar 27, 2017, at 11:45 AM, Caryn Anderton via Histonet 
>  wrote:
> 
> Hello-
> 
> I wanted to know if it was possible to post a position that I have available? 
>  See Below
> 
> Immunohistochemistry Product Manager Communications Expert- Part Time
> 
> Summary:
> The position will focus on working with three internal groups within Biocare 
> Medical:  Marketing, Engineering and R&D. We're looking for a driven, 
> resourceful technical writing and communication expert to manage the process 
> of writing our customer-facing product documentation. You'll manage the 
> end-to-end process of drafting, writing, and releasing documentation, 
> tutorials, developer guides for:  internal and client trainings, sales 
> collateral and product manuals.
> Engineering & R&D:
> Collaborate with the Engineering team to launch a new automated IHC slide 
> staining platform. Engage with the teams to understand the instrument and how 
> it works and be able to translate technical concepts, and make them easy to 
> understand for our end users. Create and maintain reference and user guides, 
> including hardware and software documentation
> Marketing:
> Work with marketing to supply scientific knowledge and technical/medical 
> writing for product catalogs, marketing brochures and website content. Work 
> with training department to develop sales training programs.
> Requirements:
> 
> ?  Broad knowledge base in Histology , Immunohistochemistry and related 
> instrumentation
> 
> ?  Excellent writing, editing, and proofreading skills with meticulous 
> attention to detail
> 
> ?  Ability to present information and generate content appropriate market 
> segments
> 
> ?  Self-motivated, independent, able to prioritize and manage multiple 
> projects
> 
> ?  Demonstrate capacity to work collaboratively within cross-functional teams
> 
> ?  Proven and consistent track record of meeting project deadlines with 
> quality results
> 
> ?  Excellent organizational and time management skills
> 
> ?  Ability to receive creative direction and feedback
> 
> ?  Please include a cover letter and curriculum-writing sample with your 
> application.
> Biocare Medical is a leading supplier of automated immunohistochemistry (IHC) 
> instrumentation, antibodies and the full range of reagents for IHC. Biocare's 
> portfolio includes novel and established primary antibodies and FISH probes 
> for many tissue types.
> EEO/AA Employer/Veterans/Disabled/Race/Ethnicity/Gender/Age
> 
> To all recruitment agencies: Biocare does not accept agency resumes. Biocare 
> will not pay for any fees related to unsolicited resumes.
> 
> Job Location-Pacheco, California, United States
> Department-Marketing
> Position Type-Part Time
> 
> 
> 
> 
> 
> 
> Caryn M. Anderton
> Senior Director of Marketing
> Biocare Medical, LLC.
> 60 Berry Dr.
> Pacheco, CA 94553
> (925) 768-1270
> www.biocare.net
> 
> 
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[Histonet] Cassette Printers

[Eileen Akemi Allison via Histonet]

Good morning Histoland! 

Our department is looking into purchasing a new cassette printer which has the 
capability of interfacing with our AP Easy LIS System. I am currently demoing a 
General Data ID/Positive CL-01 Laser Cassette Printer and label printer system. 
We are a fairly small lab with limited space so a small foot print is 
essential.  We also do not need a multiple magazine. 

I would love to hear your comments, good, bad or indifferent… 

Thanks in advance

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 

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[Histonet] cassette printer additional request

[Eileen Akemi Allison via Histonet]

Hi Again:

I forgot to mention I am open to investigate other Cassette Printers other than 
the General Data Printer… AlOf course, price, and dependability is a big 
factor. Would love to hear your comments, good, bad or indifferent...

Thanks in again!

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 

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[Histonet] RIP Wanda Platt Jones

[Eileen Akemi Allison via Histonet]

My feelings for Wanda Platt Jones: Although I am Buddhist, and believe in 
reincarnation, I am untuned to all religions! In Wanda's case, perhaps, she was 
needed to be with her GOD to help serve in the best way possible for the 
ultimate good of everyone. She had an energy and presence which was focused 
unconditionally for true GOOD and LOVE for everyone. 
Wanda to me is a True Angel; who now is looking down on us, and helping us to 
become better human beings. Anyone she came in contact with benefitted from her 
remarkable love, sweetness, and acceptance. I will truly miss her, but I know 
she has not left us. She is hugging and guiding us, and showering us with her 
love and sweetness. 
She is sitting in a Prestigious Histology Chair in the Sky with all the Greats, 
such as the likes of Chuck Churukian. He was another Great Christian 
Histologist, and a Very, Dear Friend! XoXo
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[Histonet] histology opening

[Eileen Akemi Allison via Histonet]

Hope this is acceptable. I am a Pathology Manager and am looking for a 
histologist to work for us. Here is my ad;

A Great Opportunity for West Coast Histology Students and Graduates! If you 
will be graduating, and are eligible for certification, or are recently 
graduated and certified; our GI endoscopy pathology laboratory, located in a 
private practice Gastroenterology medical group in beautiful Monterey, CA is 
expanding to hire another histologist. Our lab has a formalin-free environment 
with new microwave hybrid tissue processing technology.

We will train a knowledgeable and willing candidate. Duties to include (but not 
limited to): accessioning, data entry into AP Easy LIS System, grossing, 
embedding, microtomy, limited manual special stains, general histology and 
histology related clerical duties. The right candidate is reliable, 
self-motivated, articulate and well versed with anatomy identifications. It is 
preferred that you meet CLIA grossing regulations.

Full Time Days, Monday through Friday with no weekends. We offer full benefits 
including medical, dental and vision insurance, paid holidays, vacation and 
sick accrual and a profit sharing 401K plan. Salary is negotiable. If you are 
interested, or if you know of someone, contact me and I will give you more 
details.

Akemi Allison, BS, HT/HTL (ASCP) 
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
Work: (831) 375-3577 X117
W.Email: aalli...@montereygi.com
H. Email: akemiat3...@gmail.com
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[Histonet] New Hire for My Lab

[Eileen Akemi Allison via Histonet]

Attention West Coast Histology Students and Graduates! If you will be 
graduating, and are eligible for certification, or are recently graduated and 
certified; our GI Endoscopy laboratory in Beautiful Monterey, CA is expanding, 
and we are going to hire another histologist. We have a formalin free 
environment, with new Microwave Hybrid Tissue processing Technology.  

This is a great opportunity for the right person. WILL TRAIN a knowledgeable, 
willing candidate. Duties to include (but not limited to): accessioning, data 
entry into AP Easy LIS System, grossing, embedding, microtomy, and general 
histology *must be self motivated, reliable and a team player. Preferred that 
you meet CLIA grossing regulations. 

Monday through Friday with no weekends. We offer full benefits and 401 K. 
Salary is negotiable. If you are interested, or if you know of someone, contact 
me and I will give you more details. akemiat3377@gmail.comStudents! If you will 
be graduating and are eligible for certification, or are certified 
histologists; our GI Laboratory in Beautiful Monterey, CA is expanding, and we 
are going to hire another histologist. Preferred that you meet CLIA grossing 
regulations. 


This is a great opportunity for the right person. WILL TRAIN a knowledgeable, 
willing candidate. Duties to include (but not limited to): accessioning, data 
entry into AP Easy LIS System, grossing, embedding, microtomy, and general 
histology *must be self motivated, reliable and a team player. 


Monday through Friday with no weekends. We offer full benefits and 401 K. 
Salary is negotiable. If you are interested, or if you know of someone, contact 
me and I will give you more details. akemiat3...@gmail.com

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 
Tele: (831) 375-3577 X117


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Re: [Histonet] block retention

[Eileen Akemi Allison via Histonet]

10 years for all slides and blocks with the exception of pediatric cases. Those 
cases we keep forever.

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 
Tele: (831) 375-3577 X117
Cell: (408) 335-9994

> On Nov 8, 2016, at 8:12 AM, Teresa W. Gainey via Histonet 
>  wrote:
> 
> We keep our blocks for 15 years.  Slides foreverper Pathologist request.
> 
> 
> Teresa W.Gainey
> 
> Beaufort Memorial Hospital
> 
> Pathology Team Coordinator
> 
> 843-522-5159
> 
> 
> 
> From: Algeo, Lacie A 
> Sent: Thursday, November 3, 2016 11:57 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] block retention
> 
> Hi All,
> Outside of the current guidelines, how long is everyone saving their 
> blocks/slides.
> Thanks,
> Lacie
> 
> Lacie Algeo, HTL (ASCP) MBCM
> Histology Supervisor
> Providence Sacred Heart Medical Center Laboratory
> 101 W 8th Avenue
> L-2
> Spokane, WA 99204
> 509-474-4418
> FAX 509-474-2052
> lacie.al...@providence.org
> 
> 
> This message is intended for the sole use of the addressee, and may contain 
> information that is priviledged, confidential and exempt from disclosure 
> under applicable law.  If you are not the addressee, you are hereby notified 
> that you may not use, copy, disclose or distribute to anyone the message or 
> any information contained in the message.  If you have received this message 
> in error, please immediately advise the sender by reply e-mail and delete 
> this message.
> 
> 
> 
> 
> This message is intended for the sole use of the addressee, and may contain 
> information that is privileged, confidential and exempt from disclosure under 
> applicable law. If you are not the addressee you are hereby notified that you 
> may not use, copy, disclose, or distribute to anyone the message or any 
> information contained in the message. If you have received this message in 
> error, please immediately advise the sender by reply email and delete this 
> message.
> 
> 
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Re: [Histonet] Hourly wage for trainee in CA

[Eileen Akemi Allison via Histonet]

Jennifer:  I think you misread the inquiry. I would love to get a new graduate 
at $25.00 to $28.00 and hour.  I sent you an email to see if you had any 
candidates, but you did not respond. 

This position is considered more of a histology assistant since this person 
doesn't have a degree (only HS), no HT or HTL certification, or recent 
experience (last worked in histology in 1991). In my opinion, I think our offer 
of $20.00 an hour plus a handsome benefit's pack is not out of line. It will 
take quite a bit of training to bring her up to speed. She also won't be 
eligible to sit for her HT certification because she only has a HS education.

Akemi


> On Nov 29, 2015, at 8:14 PM, Jennifer MacDonald  wrote:
> 
> Akemi, 
> I think this offer is low for a certified HT/HTL, particularly in that area.  
> I have had graduates go up to Northern CA and the minimum starting wage that 
> they have accepted is $25.  These are brand new graduates, with minimal 
> experience, but I don't believe their titles are "trainees". 
> Jennifer 
> 
> 
> 
> From:Eileen Akemi Allison via Histonet 
>  
> To:Histonet  
> Date:11/28/2015 08:08 AM 
> Subject:[Histonet] Hourly wage for trainee in CA 
> 
> 
> 
> Hello all my histology friends in California: 
> 1st I hope you all had a wonderful Thanksgiving! I would like to know what 
> the hourly rate of a new histologist with a HT certification is making. I 
> would very much appreciate this information.  We are offering a training 
> position to a local individual who doesn't have a degree, HT or HTL 
> certification, or recent experience (last worked in histology in 1991). I 
> want to see if our offer of $20.00 an hour plus benefit's was out of line.  I 
> would prefer to have a certified HT, but no one has answered our ads.
> 
> Best regards,
> 
> Akemi Allison BS, HT/HTL (ASCP)
> Pathology Manager
> Monterey Bay GI Consultants Laboratory
> 23 Upper Ragsdale Drive, Suite 200
> Monterey, CA 93940
> W: Email: aalli...@montereygi.com <mailto:aalli...@montereygi.com 
> <mailto:aalli...@montereygi.com>>
> H: Email: akemiat3...@gmail.com <mailto:akemiat3...@gmail.com 
> <mailto:akemiat3...@gmail.com>>
> Tele: (831) 375-3577 X117
> 
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[Histonet] Hourly wage for trainee in CA

[Eileen Akemi Allison via Histonet]

Hello all my histology friends in California: 
1st I hope you all had a wonderful Thanksgiving! I would like to know what the 
hourly rate of a new histologist with a HT certification is making. I would 
very much appreciate this information.  We are offering a training position to 
a local individual who doesn't have a degree, HT or HTL certification, or 
recent experience (last worked in histology in 1991). I want to see if our 
offer of $20.00 an hour plus benefit's was out of line.  I would prefer to have 
a certified HT, but no one has answered our ads.

Best regards,

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 
Tele: (831) 375-3577 X117

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[Histonet] Hiring a histologist

[Eileen Akemi Allison via Histonet]

Attention recently graduated certified or certified eligible histologists! Our 
GI practice in Beautiful Monterey, CA is expanding, and we are going to hire 
another histologist to hone their skills under my tutelage. Must meet CLIA 
grossing regulations. We offer full benefits and 401 K. If you are interested, 
or know of someone who would be interested, contact me and I will give you more 
details. akemiat3...@gmail.com
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Re: [Histonet] Temperature for slides

[Eileen Akemi Allison via Histonet]

We monitor room temp and record it as per CAP, and CLIA.  Are you sure the 
request wasn’t for monitoring room temp because of paraffin blocks, not slides? 
The concern is for paraffin blocks melting, due to excessive heat.

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
Email: aalli...@montereygi.com 
Tele: (831) 375-3577 X117


> On Aug 28, 2015, at 4:58 AM, Heckford, Karen - SMMC-SF via Histonet 
>  wrote:
> 
> Okay I just got a weird request for monitoring the room temperature where I 
> have my slides for that I use for Histology.  We are talking blank slides.  I 
> have never heard of this before.   Who else does this???  Apparently JCAHO 
> wants this.
> 
> Karen Heckford HT ASCP CE
> Lead Histology Technician
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> karen.heckf...@dignityhealth.org
>   
>Caution:  This email message, including all content and attachments, is 
> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The 
> information contained in this email message is intended only for the use of 
> the recipient(s) named above. If the reader of this message is not the 
> intended recipient or an agent responsible for delivering it to the intended 
> recipient, you have received this document in error.  Any further review, 
> dissemination, distribution, or copying of this message is strictly 
> prohibited.  If you have received this communication in error, please notify 
> us  immediately by reply email.  Thank you."
> 
> 
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Re: [Histonet] Marciafava-Bignami Disease

[Eileen Akemi Allison via Histonet]

From my archives regarding my dear friend (Chuck)  Charles Churukian:
> December 26, 2010 
>  ·  
> 
> I just heard from Irene Churukian today. Please put Chuck in your prayers. 
> Irene said this: CLL cells changed in character so Chuck began chemo 
> treatments on Dec. 1. His second one was Dec. 21... He has six altogether 
> -The first cycle was difficult... he was very weak-in bed most of the time. 
> He is now improving-thankfully. We are praying that by April, he will be in 
> remission after all the treatments are completed. RIP Chuck Xo

Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 


> On Aug 4, 2015, at 2:11 PM, Ingles Claire via Histonet 
>  wrote:
> 
> I know I watch my exposure to Formalin and Xylene especially. I was diagnosed 
> with NASH in 2007 (who knows how long before then, since I am asymptomatic) 
> when I had a Pituitary Adenoma that had hemorraged (Boy talk about a 
> headache!) Anyway, I have always wondered about the cause. I thought it was 
> genetic as my father also had it for over 40 years before finally having a 
> transplant. But I wonder if it was his exposure to jet fuel, etc. as he was a 
> jet mechanic for 20 years in the USAF plus another 15-20 with a private 
> company. So far my liver has been stable even getting a bit better to the 
> point of being just a hair above the normal range. I do everything under the 
> sun in my lab. Mohs, Grossing, plus all the routine stuff of cutting and 
> staining, etc. I have been able so far to keep the repetitive injuries at 
> bay, and Dansco shoes have saved my feet from the Plantar Faciitis (had that 
> about a year straight at one point.). We had managed to keep xylene out of 
> our lab for years by using Propar, but one of the Pathologists insisted on 
> having a tape coverslipper. We still use it as little as possible. I had a 
> co-worker, when she was pregnant wasn't allowed to gross (hence contact with 
> formalin). Because of the NASH I get blood tests done every 6 months. It will 
> be interesting to see if they have changed possibly due to the exposure to 
> xylene, which I haven't had exposure to for years otherwise.
> Claire
> 
> 
> From: Mayer,Toysha N via Histonet [histonet@lists.utsouthwestern.edu]
> Sent: Tuesday, August 04, 2015 2:49 PM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: Re: [Histonet] Marciafava-Bignami Disease
> 
> This is very interesting.  I have worked in the field for over 20 yrs, and 
> was pregnant while working in a small lab.  Mostly everything was manual, and 
> I did not use all of the safety precautions I should have (my fault). My son 
> had severe speech and language delay as well as a language processing issue 
> when he was smaller, and now stutters.  I have often wondered (as most 
> parents would) if my lack of precautions may have contributed. We stress the 
> safety issues to the students daily, to get it all in their heads.  I often 
> tell  people that I am so happy because I work with xylene, and I feel real 
> good.  So far, I don't see any noticeable symptoms of anything wrong, just 
> the usual-decreased olfactory sensing, repetitive motion issues, plantar 
> fasciitis, and varicose veins.  Hair loss was an issue at one time, but that 
> was attributed to PCOS.  Now that I am not in the lab full time anymore it 
> has decreased since I went natural (I'm African-american).  One thing I have 
> noticed is my C-reactive protein is high, and I take a statin for that.  I 
> have no liver issues, and my functions appear to be normal.  Next time I go 
> in for a physical, I will have my workplace hazards documented in my EMR.
> I will look up Primary Biliary Cirrhosis (PBC), to get some more information, 
> especially since I was born jaundiced and I am anemic.
> 
> 
> Sincerely,
> 
> Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
> Instructor/Education Coordinator
> Program in Histotechnology
> School of Health Professions
> UT M.D. Anderson Cancer Center
> 713.563-3481
> 
> 
> Message: 11
> Date: Tue, 4 Aug 2015 06:30:12 -0700
> From: Eileen Akemi Allison 
> To: "Edmondson David (RBV) NHS Christie Tr"
>
> Cc: Histonet 
> Subject: Re: [Histonet] Marciafava-Bignami Disease I have re-logged
>into thesystem and maybe this will now communicate with 
> Histologists
>out there,  and hope that I do not get twice as many emails from
>Histonet#
> Message-ID: 
> Content-Type: text/plain;   charset=utf-8
> 
> GP?s are fine for general health issues, but I would certainly get more 
> conclusive tests done by a neurologist, as well as contacting the best 
> worker?s comp attorney in your area who has dealt with chemical exposure 
> cases.
> 
> Studies show w

Re: [Histonet] Marciafava-Bignami Disease I have re-logged into the system and maybe this will now communicate with Histologists out there, and hope that I do not get twice as many emails from Histone

[Eileen Akemi Allison via Histonet]

GP’s are fine for general health issues, but I would certainly get more 
conclusive tests done by a neurologist, as well as contacting the best worker’s 
comp attorney in your area who has dealt with chemical exposure cases.

Studies show we are #1 in this country for the most hazardous professions. It's 
safe compared to when I 1st started in this field in 1965! Well, I am a 
dinosaur from back in the day when we had inadequate ventilation, a shortage of 
fume hoods, inadequate education on hazards, safety and PPE's. 

In 1979 I started to work at OHSU in the surgical path lab, as well as doing 
research projects. We made up all of our own H&E's and special stains from 
scratch, as well as made up our own 10% NBF in 55 gallon drums without fume 
hoods, ventilation, masks or gloves. I also worked with Glyco-Methacrylate 
embedded tissues without hoods or gloves! Since it was a medical school, we did 
every special stain under the sun and dealt with about every chemical, reagent, 
acid and stain you could think of! We also smoked cigarettes and drank coffee 
in the lab while we embedded and cut! We sure were a naive group back then!

In the early days, the facilities I worked in never had MSDS information 
available. In 1989, while at Emanuel Hospital, Portland, OR, I researched and 
compiled the MSDS information on all the chemicals, reagents, acids and stains 
that we used. That was the 1st eye opener to what me and my fellow histologists 
dealt with on a daily basis. 

In 1988 I had base line tests done because I was having issues with dizziness, 
balance, reflexes, and short term memory loss. In 1992, I had extensive 
neurological tests done, as well as a sural nerve bx taken from my right ankle. 
It was found that I had nerve damage, loss of balance, no reflexes, numbness in 
my fingers, hyper sensitivity and reduced feeling on my right side, hearing 
loss in my left ear, and an aedes pupil in my right eye. It was concluded these 
were the results from exposure to multiple toxic chemicals in an extremely 
small room with excessive heat and NO VENTILATION at current hospital I was 
working at. These health issues are irreversible. I just deal with it. I was 
the 1st person who won a case for this in the state of Oregon, but it had 
consequences. I won the battle, but lost the war!  

I now have Primary Biliary Cirrhosis (PBC).  I was 1st diagnosed in 2008 for 
PBC. I think this condition was caused by my continual exposure to Multiple 
Toxic Chemicals. You may, or may not agree. A huge amount of the chemicals we 
deal with in the histology lab targets the liver and is absorbed through the 
skin or is inhaled. Here is the link for PBC. 
http://www.liverfoundation.org/abouttheliver/info/pbc/ 


Akemi Allison BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 


> On Aug 4, 2015, at 4:47 AM, Edmondson David (RBV) NHS Christie Tr via 
> Histonet  wrote:
> 
> __
>From:   Edmondson David (RBV) NHS Christie Tr
>Sent:   02 August 2015 16:40
>To: 'histonet@lists.utsouthwestern.edu'
>Subject:Marciafava-Bignami Disease
> 
> Hello out there,
> I have been working in Histology for forty years and am wondering at a cause 
> for this disease.  It was seen on MRI scans and they have told me to ease up 
> on the alcohol, but I have never been remotely alcholic so I am giving up 
> work in October and hope to live a little longer.  Addition to work has been 
> my only problem.
> 
> They have tested a variety of bodily functions, touching my nose and foot 
> movements together with assessing my ability to ride my motorcycle and make 
> toast and tea, and then they let me off the acute neuro ward.
> My bike was chained up outside and making my way between lines of static 
> traffic should have been a good sign.
> 
> I am currently on massive doses of B vitamins but my GP reckons that they are 
> of no use and I should just get better.
> 
> So, is Xylene at the root of all my demyelination??
> 
> Regards
> 
> David Edmondson
> 
> 
> 
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