[Histonet] PDL-1/PD-1

[Bell, Pat]

Hello to everyone!

Has anyone used PDL-1/PD-1 on FFPE tissue?  Could I please get a copy of your 
protocol and where you purchased the antibody?

Thank you so much.
Pat

Pat Bell HT(ASCP)
Sr. PRA
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave
Aurora, Colorado 80045
303-724-6077
pat.b...@ucdenver.edu

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[Histonet] RE: dish drying wall mount

[Bell, Pat]

VWR has one.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Wednesday, September 04, 2013 9:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] dish drying wall mount


Does anyone know where I can find a wall mount that you put your clean dishes 
on to dry?  We currently just lay a paper towel on the cabinet to let our 
dishes dry on, but I'd like to find a the wall mount that has areas where you 
can hang the items up to dry.  Thanks!

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[Histonet] iNOS

[Bell, Pat]

Hello to Everyone,

Does anyone have an antibody for iNOS that will work in Human? I appreciate any 
help you wonderful people could give me. Also, could you share your protocol 
with me?

Thanks,
Pat

Pat Bell HT(ASCP)
Medical Oncology
University of Colorado, Denver
12801 E 17th Ave, MS 8117
Aurora, Colorado 80045
303-724-6077
pat.b...@ucdenver.edu

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RE: [Histonet] BRAF IHC

[Bell, Pat]

OH, Wrong Pat! :)

-Original Message-
From: Susan Foreman [mailto:sfore...@labpath.com] 
Sent: Wednesday, July 10, 2013 11:05 AM
To: Bell, Pat; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] BRAF IHC

I was attempting to respond to this older post:   Thanks

Pat Zeitlow pzeitlow <@t> bbpllab.com
Fri May 3 13:52:49 CDT 2013

Previous message: [Histonet] BRAF IHC
Next message: [Histonet] BrDU
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Also using Spring Bioscience antibody.  Trying to get it automated using 
Biocare instrument.  You?

Pat



-Original Message-
From: Bitting, Angela K. [mailto:akbitting <@t> geisinger.edu]
Sent: Friday, May 03, 2013 1:45 PM
To: Pat Zeitlow
Subject: RE: BRAF IHC

Working it up right now. Using Spring Bioscience's BRAF V600E.  Are you using 
an automated platform? 

 Angie 

-Original Message-
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces 
<@t> lists.utsouthwestern.edu] On Behalf Of Pat Zeitlow
Sent: Friday, May 03, 2013 2:36 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] BRAF IHC

Is anyone doing BRAF IHC staining that is willing to share protocol details?

TIA,

Pat




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RE: [Histonet] BRAF IHC

[Bell, Pat]

I have not used this antibody so I don't know what you mean. Maybe I am missing 
something!

Sorry,
Pat

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Susan Foreman
Sent: Wednesday, July 10, 2013 10:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] BRAF IHC

Pat,

   I was wondering how your BRAF IHC staining is going?  We have the same 
antibody working using Ventana Benchmark Ultra.  We do use amp kit.  With you 
post, did anyone respond that was using other clones?  V600K or D?

 

Many Thanks,

Susan Foreman

(865)584-1933

 

 

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[Histonet] CK5 and p63

[Bell, Pat]

Hello to Everyone,

Has anyone had experience doing CK5 and p63 in the rat? Could you please tell 
me where you get your antibody? I currently use Biocare's for human and will 
try it this week on the rat. I just was wondering if anyone had any other ideas.

Thank you for your help.
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology
12801 E 17th Ave, MS8117
Aurora, Colorado 80045
303-724-6077
pat.b...@ucdenver.edu

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[Histonet] COX2 in Human

[Bell, Pat]

Hello to all!

I have been doing COX2 in human breast tissue and normally it works great. 
Lately, however, I have been getting a lot of background and nonspecific 
staining even with a new lot. Does anyone might know what I am doing wrong and 
how I could correct this problem?

Thank you and HAPPY LAB WEEK!!!
Pat

Pat Bell (HT-ASCP)
University of Colorado, Denver
Medical Oncology
12801 E 17th Ave, MS 8117
Aurora, Colorado 80045
303-724-6077
pat.b...@ucdenver.edu

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[Histonet] CD11b

[Bell, Pat]

Hello to Everyone!! :)

Has anyone worked up CD11b in human tissue? We have tried one from Novus and 
one from Serotec with no staining or staining that was not specific. I would 
appreciate any help anyone could give! :)

Thank you,
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu

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[Histonet] RE: Cold room alternative

[Bell, Pat]

Aaron,

We have a refrigerator from VWR that has a glass door like a "Deli" frig. and 
it has an electrical outlet inside as well as outside. That could be a starting 
place to look. :)

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Johnson, Aaron
Sent: Tuesday, October 02, 2012 3:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cold room alternative

The building I am in does not have a cold room and I would like to incubate 
some samples overnight on an orbital shaker at 4°C. Can anyone recommend a 
refrigerator with an interior electrical outlet or another alternative? Thanks.
Aaron

Aaron M. Johnson
Assistant Professor
Department of Speech and Hearing Science University of Illinois at 
Urbana-Champaign

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[Histonet] RE: Slide printing systems

[Bell, Pat]

I also have the slide-mate and have a lot of problems with the printing. We 
also have to spend a lot of time rewriting and labeling them. I have been told 
to send it in to get it repaired but I wonder if it is worth the time and money 
if you are getting it back in basically the same condition! I know the Leica is 
pretty good but I am in research and can't spend that kind of money. I wish 
there was a system that was good and not extremely expensive. 

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell, 
Janice A
Sent: Wednesday, August 15, 2012 8:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slide printing systems

Good Morning,

We currently are using Thermo-Fisher print-mate and slide-mate.  I am not very 
happy with the printing of the slides.  Some days I feel like spend most of my 
time re-writing and labeling printed slides. Then when the units  are  sent out 
for repair and it arrives back it is not a whole lot better.  Before we get our 
new LIS system and our Docs will be scanning the bar code(that sometimes do not 
print) I need to find a better system.  I will not be able to deal with how 
often my name will be called out when the scanning of the slides fail.

I would like any input on other systems pros and cons that are currently being 
used in histology labs.   I know no system will be perfect but something has to 
be better.

Thanks, Janice

Janice A. Mitchell, BS, HT(ASCP)
Histology Lead Tech
Children's Hospital of Philadelphia
Anatomic Pathology and Laboratory Medicine
324 S. 34th Street
Philadelphia, Pa 19104-4399
215-590-1738(lab)
267-426-7754(office)


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[Histonet] Fixation and Processing of Mouse Lactating Mammary Gland

[Bell, Pat]

Hello to all,

I have some lactating mouse mammary tissue that was fixed in 10% formalin for 
24 hours and then stored in 70% alcohol for 1 day before processing. The 
processing is as follows: 80%-30min, 95%-30min, 95%-40 min, 100% X3-30 min. 
each, xylene X3-40min. each, and paraffin X3-45min. each. The tissue if very 
mushy and I am wondering if the tissue was fixed long enough? The tissue is 
approximately 3mm in thickness but because of the milk in the tissue does it 
need to fix more or should I process longer?

I have embedded the tissue and am wondering how I should go about reprocessing 
the tissue. Should I go back through xylene to alcohol and then fix for longer 
and then reprocess or can I melt the blocks and put in fix and then reprocess? 
I have heard both ways but want to do the method that will not comprise the IHC 
that needs to be done.

Thank you so very much for all of your input and help.
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu

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[Histonet] Alpha V Integrin

[Bell, Pat]

Hello to Everyone and Happy New Year,

Does anyone know of an antibody for  Alpha V Integrin for IHC that will work in 
mouse tissue?

Thank you for all of your help.
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu

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RE: [Histonet] QIHC

[Bell, Pat]

Thank you to everyone for all of your help.

I appreciate it very much.
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu
 

-Original Message-
From: Montina Van Meter [mailto:montina.vanme...@pbrc.edu] 
Sent: Wednesday, February 17, 2010 1:33 PM
To: Bell, Pat; Walter Benton; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] QIHC

Here is Ethel's contact information:


Ethel R. Macrea, HT(ASCP)QIHC  
Ventana Medical Systems  
HC 3 Box 1023, 22191 S. Branch Road  
Tucson, AZ 85739  
UNITED STATES 
Phone: (520)219-8471  
Fax: (502)219-8454  

Email: dawnb...@earthlink.net  









Montina J. Van Meter, HT (ASCP)
Lab Manager
Autonomic Neuroscience
Pennington Biomedical Research Center
6400 Perkins Rd.
Baton Rouge, LA  70791
225-763-2564

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bell,
Pat
Sent: Wednesday, February 17, 2010 1:41 PM
To: 'Walter Benton'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] QIHC

I tried to email Ethel at this email address and was told that it was
not a working email address and was "undeliverable". 

Also, what NSH study guide did you use?

Thank you,
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter
Benton
Sent: Monday, February 15, 2010 7:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] QIHC

I recently took and passed the examination . I used the Dako book,
material from Ethel Macrea's presentation, NSH study guide and material
from the other references on the list. 
 
Ethel can be reached at emac...@swskinpath.com 
 
She is a wonderful resource. 
 
 
Message: 6
Date: Fri, 12 Feb 2010 20:22:01 -0500
From: jfi...@aol.com 
Subject: [Histonet] QIHC
To: histonet@lists.utsouthwestern.edu 
Message-ID: <8cc7a6213f16820-47fc-8...@webmail-m018.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
 

Hello, I was wondering if anyone out there had any tips on passing the
QIHC, I have studied with the Dako book but unfortunately I did not pass
at my first attempt. If there are any sample questions or packages
available I would really like to get them.
Thanks
fj
 
 
 
 
Walter Benton, HT(ASCP)QIHC
Histology Supervisor
University of Maryland Medical Center
Anatomic Pathology
22 S. Greene St 
Room NBW65
Baltimore MD 21201
(Direct) 410-328-0930
(Lab) 410-328-5524
(Fax) 410-328-5508

 

This e-mail and any accompanying attachments may be privileged,
confidential, contain protected health information about an identified
patient or be otherwise protected from disclosure. State and federal law
protect the confidentiality of this information. If the reader of this
message is not the intended recipient; you are prohibited from using,
disclosing, reproducing or distributing this information; you should
immediately notify the sender by telephone or e-mail and delete this
e-mail.

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RE: [Histonet] QIHC

[Bell, Pat]

I tried to email Ethel at this email address and was told that it was not a 
working email address and was "undeliverable". 

Also, what NSH study guide did you use?

Thank you,
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
RC1-S, L18-8402C
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu
 
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Walter Benton
Sent: Monday, February 15, 2010 7:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] QIHC

I recently took and passed the examination . I used the Dako book, material 
from Ethel Macrea's presentation, NSH study guide and material from the other 
references on the list. 
 
Ethel can be reached at emac...@swskinpath.com 
 
She is a wonderful resource. 
 
 
Message: 6
Date: Fri, 12 Feb 2010 20:22:01 -0500
From: jfi...@aol.com 
Subject: [Histonet] QIHC
To: histonet@lists.utsouthwestern.edu 
Message-ID: <8cc7a6213f16820-47fc-8...@webmail-m018.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"
 

Hello, I was wondering if anyone out there had any tips on passing the QIHC, I 
have studied with the Dako book but unfortunately I did not pass at my first 
attempt. If there are any sample questions or packages available I would really 
like to get them.
Thanks
fj
 
 
 
 
Walter Benton, HT(ASCP)QIHC
Histology Supervisor
University of Maryland Medical Center
Anatomic Pathology
22 S. Greene St 
Room NBW65
Baltimore MD 21201
(Direct) 410-328-0930
(Lab) 410-328-5524
(Fax) 410-328-5508

 

This e-mail and any accompanying attachments may be privileged, confidential, 
contain protected health information about an identified patient or be 
otherwise protected from disclosure. State and federal law protect the 
confidentiality of this information. If the reader of this message is not the 
intended recipient; you are prohibited from using, disclosing, reproducing or 
distributing this information; you should immediately notify the sender by 
telephone or e-mail and delete this e-mail.

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[Histonet] Vimentin for Mouse

[Bell, Pat]

Thank you all very much for your help regarding the COX2 for the mouse. Now I 
would like to ask your help regarding Vimentin for FFPE mouse tissue. I have 
tried Dako and Sigma with no success. I am also not sure of what clone to use.

Thanks again.
Pat


Pat Bell HT(ASCP)
Medical Oncology; MS 8117
12801 E 17th Ave.
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu


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[Histonet] COX2 in mice

[Bell, Pat]

Does anyone know of a good antibody for COX2 that works in the mouse?

Thank you,
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu


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RE: [Histonet] Sakura Products

[Bell, Pat]

Just don't buy the slide printer. 

Pat Bell, HT(ASCP)
University of Colorado Denver



From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley 
[azdud...@hotmail.com]
Sent: Wednesday, August 26, 2009 10:24 AM
To: em...@pathlabsolutions.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Sakura Products

emily,  we have a sakura processor, embedding center, an accu-cut SRM microtome 
and a coverslipper.  they are all great. you can't go wrong with sakura.  anita

> Date: Tue, 25 Aug 2009 08:54:22 -0400
> From: em...@pathlabsolutions.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Sakura Products
>
> Hi All:
>
> I'm looking to purchase equipment for a new lab and would like the opinion
> of anyone who has worked with various Sakura products before... good or bad.
> I would be looking to buy the following products:
>
> Tissue-Tek TEC 5 Embedding Station
> Accu-Cut SRM 200 Microtome
> Cyto-Tek Cytocentrifuge
>
> Also if anyone has experience with prostate and GI bx processed in the
> Tissue-Tek Paraform Sectional Cassette System, I'd appreciate input on that
> too.
>
> Thanks!
>
> --
> Emily Butte, M.S., HTL (ASCP)
> Path Lab Solutions Inc.
> (701) 371-7515
> em...@pathlabsolutions.com
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[Histonet] RNA In Situ Hybridizaton

[Bell, Pat]

My supervisor wants me to learn In Situ Hybridization using RNA probes in 
formalin fixed, paraffin embedded tissues.  I have a Dako Autostainer, but I 
know that some of the procedure would be manual.

I would appreciate any protocols that would help me.

Thank you for your help.
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu


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[Histonet] RNA In Situ Hybridization

[Bell, Pat]

I want to learn to do In Situ Hybridization using RNA probes in formalin fixed, 
paraffin embedded tissues. I have the Dako Autostainer, but I know that some of 
the procedure would be done manually.

I would appreciate any protocols that would help me.

Thank you for your help.
Pat

Pat Bell HT(ASCP)
Medical Oncology; MS 8117
12801 E 17th Ave.
Aurora, Co. 80045
303-724-6077
pat.b...@ucdenver.edu


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