RE: [Histonet] (no subject)
Hi Clay, Most hospitals have a tissue committee that makes decisions about surgical specimens. These decisions become hospital policy. Most hospitals state that once a specimen is removed it essentially becomes the property of the hospital. Release of specimens is usually after it has been accessioned and grossed in for pathology reveiw. There is usually a form for that type of release. The hospital would be at risk if they let the specimen leave without following hospital policy. I am not saying it is right or wrong but I can see the logic in it. Once the specimen has been processed through the hospitals pathology department then blocks and slides can be requested for 2nd opinions. Good luck in your search for answers. Christie Gowan To: histonet@lists.utsouthwestern.edu Date: Thu, 12 Sep 2013 12:38:10 + From: claymi...@hotmail.com Subject: [Histonet] (no subject) I need a little help on a Patient’s rights question. It is my understanding that when a patient has a procedure, the patient has the right to request that those specimens be examined by a laboratory of their choosing. i.e. EGD, colonoscopy, etc I am in Arkansas. My father had a procedure yesterday at a local hospital. I manage a pathology laboratory that specializes in the type of tissue that the procedure procured. When it was requested that the tissue be sent to my laboratory, the hospital staff refused to fulfill the request. We asked multiple times for a release form so my father could take his tissue with him. The administration employee we spoke to said there was no such thing and that patients were not allowed to take their specimens. One employee going so far as stating that if we wanted the tissue sent somewhere aside from their contracted laboratory, that the procedure would be canceled and my father would have to go somewhere else. My father, not wanting to cause a fuss, let the issue go. Questions: Is it legal for a hospital to require that tissue specimens be sent to a lab they are contracted with? Are there any other actions we could have taken to make our requests be honored? This is not meant to insult the hospital, but to give an explanation of the situation for context in answering the questions. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alport Syndrome
Is anyone using the Fluorchrome-Conjugated MoAbs for Alport Syndrome distributed by Cosmo Bio Co., LTD.? We are using it on cryostat sections of renal tissue and the Texas Red fluorochrome seems to be extremely robust while the FITC-conjugated-anti alpha2(IV) is weak. I would be interested to hear your outcomes/solutions. Christie Gowan HT (ASCP) UAB Hospital Surgical Pathology Supervisor Lab Services 205-934-4991 cgo...@uabmc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TBS Slide Printer
I am posting a question for a friend. She is looking for feedback on the TBS Shurmark slide marking instrument. All comments are appreciated. Thanks. Christie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dako vs Ventana IHC systems
Hi Joe, When searching for an automated IHC stainer, you need to shop based on your facility and it's needs. We recently went through the whole shopping around experience and because we are such a large facility, we have the Ventana Ultra as well as an XT and the New Dako Link. We use the Ventana Ultra for single piece flow and it really changed the dynamic of our lab. We also use the Ventana for all our automated probes including the dual Her2/Chr17 CISH. The Dako's (we have 2) we use for all those things that we do not run on the Ultra or Benchmark XT. About 50/50 on each system. We also do a lot of research for the UAB campus and it is nice to have the more open DAKO systems. We have 3 very experienced IHC techs working in the lab which lends a lot of consistency to the staining. I say this because if you rotate people through the lab or your staff is less experienced then you want something that is easy and consistent. We will probably always have the Ventana systems in our lab so not much shopping around for a more or less closed system (there is always debate over the open/closed thing) However, shopping around for a more open system can be a little harder. We demo'ed the bond, Biocare, Dako and Labvision. We narrowed it down to Biocare and Dako based on ease of use, speed and capacity and reagents/antibodies. Once we had narrowed it down to the 2 companies, then we started looking at packages. Dako delivered the best package and because Dako already had a presence in our Histology lab (Artisan Special Stainer) and we knew their service was always prompt (we always struggled with this with Leica) We chose Dako and replaced our 2 older Labvisions with Autostainer Links. If you have any questions you can let me know via email and I will give you our contact info. Best of luck to you. Christie Gowan UAB Hospital Birmingham, AL > From: joewal...@rrmc.org > To: terri.br...@northside.com; lseb...@uwhealth.org; > lynn.bur...@illinois.gov; gth...@pcasoutheast.com; > histonet@lists.utsouthwestern.edu > Date: Mon, 29 Oct 2012 14:38:20 + > Subject: RE: [Histonet] Dako vs Ventana IHC systems > CC: > > Which Dako and Ventana machines are you all using? We are in the market for a > new IHC stainer and the new Ventana BenckMark Ultra machine looks interesting. > > Joe W. Walker, Jr. MS, SCT(ASCP)CM > Anatomical Pathology Manager > Rutland Regional Medical Center > 160 Allen Street, Rutland, VT 05701 > P: 802.747.1790 F: 802.747.6525 > NEW EMAIL: joewal...@rrmc.org > www.rrmc.org > > Our Vision: > To be the Best Community Healthcare System in New England > > Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet > Recognition® and the Governor's Award for Performance Excellence > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Brown > Sent: Monday, October 29, 2012 10:26 AM > To: Sebree Linda A; Burton, Lynn; Gloria Tharp; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Dako vs Ventana IHC systems > > We had Ventana and changed to Dako. The Dako phone tech support is > excellent and we never wait long for in house service. Our > pathologists love the turnaround time with the open system and the stain > quality. > > Terri H. Brown, HT (ASCP) > Pathology Laboratory Manager > Northside Hospital Atlanta > terri.br...@northside.com > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A > Sent: Friday, October 26, 2012 4:52 PM > To: 'Burton, Lynn'; Gloria Tharp; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Dako vs Ventana IHC systems > > I concur with Lynn; we've been dealing with Ventana 19 years. > > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn > Sent: Friday, October 26, 2012 3:47 PM > To: Gloria Tharp; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Dako vs Ventana IHC systems > > We have been dealing with Ventana for at least 10 years with very good > service and reliability. Their phone tech support is excellent and we have > never waited long for in house service. > Lynn Burton > Lab Associate > Animal Disease Lab > Galesburg, Il > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp > Sent: Friday, October 26, 2012 2:38 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Dako vs Ventana I
RE: [Histonet] Productivity
Hi Karen, The best way to communicate with management is through data. If you have access to say the last 3 years of blocks and or slides cut and you compare it to accessions or cpt codes then you have a roadmap of where you have been in the past and where you are now. Do you have more or less employees and is this a temporary lull in numbers or has this dip occurred before. At least you will be able to formulate a more informed response to their request for cutting back hours. This is always a complicated issue and we have seen an increase in case complexity as well in our institution so our numbers of blocks vs. specimen count is skewed as well. Tell them you will cut hours but you will need all new equipment to continue to meet your deadlines! Ha!. Let us know how it turns out and good luck! Christie Gowan, HT (ASCP) UAB Hospital Histology Supervisor Surgical Pathology 205 934 4991 cgo...@uabmc.edu > From: karen.heckf...@dignityhealth.org > To: histonet@lists.utsouthwestern.edu > Date: Mon, 20 Aug 2012 10:42:39 -0700 > Subject: [Histonet] Productivity > > Good Morning, > I know we have been over this before, how does your lab measure productivity? > Are you going by block and slide or by CPT code? I have been told by the > "Suits" that we can only measure productivity by per specimen or CPT code > with no weight and value. As you know when you get a big case you can have a > bunch of blocks on it therefore a bunch of slides. I do everything from GI > specimens to big breast cases. They will tell me that our productivity is > down and we need to cut hours. I just do not know exactly how to drill it in > to them that things are not always as they appear on paper. I am talking in > circles until I am blue in the face...AUGH!! > > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > karen.heckf...@dignityhealth.org > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the intended > recipient, you have received this document in error. Any further review, > dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please notify > us immediately by reply email. Thank you." > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Symphony Issues
Hi Nita, We had a similar problem and our Technical Service rep narrowed it down to where the coverslippers were actually manufactured. After changing out the coverslipper 3 times, he ordered a new one directly from Tuscon and we have not had the problem again. So far as humidity goes, there could be a number of issues. The first thing you need to check is with your engineers to make sure they are not pumping moisture into the air. Many hospitals do this so that is where I would start. Also, keep that filter changed regularly. I wish you luck and hope you can make it work for you. Christie Date: Tue, 31 Jul 2012 11:05:41 -0500 From: nsea...@swmail.sw.org To: histonet@lists.utsouthwestern.edu; pwebs...@swmail.sw.org CC: Subject: [Histonet] Symphony Issues This inquiry is from the lead histotech inour lab: Is anyone having problems with moisture on the slide, bubbles and misaligned coverslips or multiple coverslips. If so, what are they being told from Ventana? What have they done to resolve issues? Can you respond to her directly @ pwebs...@swmail.sw.org Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsea...@swmail.sw.org 254-724-2438 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Inking Skin
Thanks for all the info everyone. The tattoo ink sounds intriguing. We don't seem to have a problem getting full sections on any other derm specimens so I think it is specimen specific or technique specific meaning that the epithelial layer of these samples vary in thickness so I will keep you all posted as to the outcome as I know you are all waiting with anticipation Thanks again Christie > Date: Sat, 28 Jul 2012 13:49:23 -0400 > From: rsrichm...@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Re: Inking Skin > > Christie Gowan at UAB Hospital. Birmingham, Alabama asks: > > >>My derm pathologist has requested that we ink the epithelium on MOHs derm > >>cases (new pathologist). We process many derm specimens a day but it seems > >>we are having trouble getting full epithelium on our cases that come over > >>from the MOHs clinic. I am not sure that inking the skin will help with the > >>problem but let's just say that if we do decide to ink the surface of these > >>specimens, does anyone know of an ink that will actually stick to skin? > > Your new pathologist should be able to specify what he wants. About > time they started teaching pathology residents where slides come from. > > Mohs is somebody's name, not an acronym. Specifically Frederic Mohs, > an American surgeon (1910-2002): > http://en.wikipedia.org/wiki/Frederic_E._Mohs > > You need a particulate ink that will stick to the specimen, withstand > frozen section or paraffin processing, and be visible on the slide. > > Particulate marking inks for surgical pathology are made by a variety > of manufacturers. I prefer the Davidson marking inks, but there are > several other brands. They come in at least seven colors. A wooden > case that keeps the tall narrow bottles from tipping over is usually > included. > > Tattoo inks are cheap and come in an almost unlimited variety of > colors, and in the one lab I've used them in work extremely well. The > downside is you have to read some pretty appalling catalogs if you > don't like tattoos and piercings (gimme a break, I'm 73 years old.) > > You can get ordinary India ink (which by definition is black) very > easily. I didn't know that craft stores like Hobby Lobby offer a > variety of colored particulate inks - I'd want to know exactly what to > buy, to make sure I got a suitable colored ink that would show up on > the slide. > > All of these inks have a good shelf life if you keep them tightly > capped except when you're actually using them. If they become > excessively viscous or solidify, they need to be thrown out. > > The specimen must be blotted thoroughly dry before inking, and the ink > blotted off afterwards. If you do this you won't need to use anything > to "set" the ink. I never use these fixatives. If you do want to use > such a fixing solution, 2 or 3% acetic acid (diluted from glacial > acetic acid) or half strength ordinary grocery store white vinegar is > supposed to work as well as anything. It's unnecessary to use Bouin's > fixative or acetone, both of which are serious hazmats. > > Bob Richmond > Samurai Pathologist > Knoxville TN > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Inking Skin
Just to clarify: We do ink our excisions as described below in Cindi's response howerver, our MOHs specimens come to us mounted on a pieces of sturdy paper and are processed in place. We only describe them at gross and then directly place the paper and skin into a cassette for processing. The specemins are skin side up and then placed on edge when embedded. The clinician has stated that he will ink the skin for us so we have a better success rate at getting a full epithelial section at microtomy. Thanks Cindi for your input. We actually use a diluted acetic acid solution to set our inks which is the same thing as vinegar. Christie > Date: Fri, 27 Jul 2012 15:38:49 -0400 > From: robin...@mercyhealth.com > To: christiego...@msn.com > Subject: Re: [Histonet] Inking Skin > > We use cotton swabs dipped in india ink in a variety of colors (purchased > from the local Hobby Lobby) and set it with vinegar. We ink the resected > margin not the skin. If there is no orientation we use black. If we have > orientation we use at least three colors. The ellipse is inked with one color > from tip to tip on one half. The other half is divided between two colors. We > cut perpendicular to the side with the solid color so each cross section has > two colors on it. > > > Cindi Robinson HT(ASCP) > Mercy Medical Center > Dunes Medical Laboratories > 350 W Anchor Dr > Dakota Dunes SD 57049 > phone-712-279-2768 > robin...@mercyhealth.com > > > >>> CHRISTIE GOWAN 7/27/2012 2:25 PM >>> > > Dear Colleagues, > My derm pathologist has requested that we ink the epithelium on MOHs derm > cases (new pathologist). We process many derm specimens a day but it seems we > are having trouble getting full epithelium on our cases that come over from > the MOHs clinic. I am not sure that inking the skin will help with the > problem but let's just say that if we do decide to ink the surface of these > specimens, does anyone know of an ink that will actually stick to skin? I > would appreciate anyones input. Thanks and have a great weekend. > Christie Gowan > UAB Hospital > Birmingham, Alabama ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Inking Skin
Dear Colleagues, My derm pathologist has requested that we ink the epithelium on MOHs derm cases (new pathologist). We process many derm specimens a day but it seems we are having trouble getting full epithelium on our cases that come over from the MOHs clinic. I am not sure that inking the skin will help with the problem but let's just say that if we do decide to ink the surface of these specimens, does anyone know of an ink that will actually stick to skin? I would appreciate anyones input. Thanks and have a great weekend. Christie Gowan UAB Hospital Birmingham, Alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re[2]:
Their email accounts have been hacked and all their addresses stolen. They need to report and change email login and password. This happened to me last year and I was sending out Viagra deals to all my contacts including histonet. > Date: Tue, 27 Mar 2012 12:12:23 -0500 > From: ander...@umn.edu > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re[2]: > > I agree~~they need to be deleted from Histonet !! Getting them several > times daily ! > > > On 3/27/2012 12:05 PM, Kara Lee wrote: > > I am getting these constantly as well. Very annoying. > > > >> Date: Tue, 27 Mar 2012 11:58:39 -0500 > >> From: jmitch...@uwhealth.org > >> To: histonet@lists.utsouthwestern.edu > >> Subject: RE: [Histonet] Re[2]: > >> > >> How about these postings to histonet? Is everyone else getting these > >> from Tuyen Nguyen& Diamond Ranch Music? If so - can these people/email > >> addresses be deleted from the histonet service? > >> > >> A little redneck humor is one thing - but the love boutique sex shop is > >> another. > >> > >> Jean Mitchell > >> > >> -Original Message- > >> From: histonet-boun...@lists.utsouthwestern.edu > >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tuyen > >> Nguyen > >> Sent: Tuesday, March 27, 2012 11:23 AM > >> To: histonet@lists.utsouthwestern.edu; boost...@diamondranchmusic.org > >> Subject: [Histonet] Re[2]: > >> > >> > >> Welcome, friend! > >> http://loveboutiquesexshop.com/contact.php?yfupu=65&ugazylycy=167&myroli > >> ma=28 > >> > >> ___ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> ___ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana XT Closed System misnomer
Eric, I wouldn't say my mind is closed on the issue and I appreciate your thoughts and opinions. I agree wholeheartedly about the advantages of the XT and to say that the Benchmark XT is a closed system is simply to state that it is more "closed" regarding reagents than some systems (open) where the reagents are purchsed from various vendors in whatever form you choose. Some people who use all non-Ventana antibodies may find this as a "con" vs. a "pro" when it comes to considering purchasing an XT for their lab. Thanks. Christie Gowan HT(ASCP) > Date: Thu, 16 Feb 2012 15:41:35 -0500 > From: gagn...@kgh.kari.net > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Ventana XT Closed System misnomer > > Responding to Del who asked about pros/cons of Ventana BenchMark XT: > > > > Ventana is often equated with 'closed system'. This is a misnomer. The > only thing I regard as closed (besides some colleagues' minds on this > issue) is the detection system kit and bulk reagents that are used on > the instrument. While these are proprietary, they are also of extremely > high quality, resiliency, consistency, and in my opinion, dependability. > Preparing one's own reagents can lead to a lack of consistency of > staining, and we have confidence in Ventana reagents. > > > > As others have correctly noted...other non-Ventana antibodies can be > used in Ventana dispensers. We use an equal mix of ready-to-use > antibodies with our own diluted antibodies from other suppliers. How is > this a closed system? > > > > Pre-treatments can also be used in Ventana dispensers. There is a range > of non-ab reagents such as hematoxylins and proteases. There are a > range of detection system kits as well - at least four that I can think > of. > > > > Perhaps this sounds like my personal crusade, as a Ventana user; to try > to demolish the 'closed system' myth. In my professional interactions > with non-Ventana users, this is often heard as a derogatory, negative > reaction to Ventana, which I certainly speak truth to at every > opportunity. > > > > Del, we have found the BenchMark XT's very reliable, with good support > and service, plus excellent quality reagents including antibodies. > > > > Eric Gagnon MLT > > Histology Laboratory > > Kingston General Hospital, > > Kingson, Ontario, Canada > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana xt
Pros: Lends itself well to labs that do basic IHC staining Antibodies are pre-diluted so no guess work on dilutions Good for batching runs Consistent quality of slides 24 hour technical support Can run molecular probes protocols are easy to adjust Cons: Closed system Must use Ventana antibodies or purchase special dispensers if using non Ventana products Pre-dilute antibodies are pricey Stand alone instrument so must have space for it I'm sure there is more but you just really need to see what your needs are. We have the XT and Ultra as well as open systems. We love the Ventana's but we also will always have open platforms because we do a lot of research. The work flow is another thing you need to look at. How many slides do you turn out in one day? What is your turn around time? The XT is a great instrument but it depends on your lab. Hope this helps. Christie Gowan UAB Hospital > From: dphill...@vetmed.lsu.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 16 Feb 2012 12:29:37 -0600 > Subject: [Histonet] Ventana xt > > Looking for any pros and cons on the Ventana XT. > > > > Thanks > > Del > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Music in the Laboratory
Hi All, Our lab is governed by hospital regulations that no headphones or earbuds be worn at anytime. This is considered a safety issue. We do have policies governing codes for behavior such as dress, hygiene, innappropriate computer use such as streaming music or social media but music is something they allow idividual departments to dictate. Our lab has decided to allow desk top radios to be played. I have a few techs that like to listen to talk radio or music so they each have their individual radios set so low that only they can hear it. I think if everyone had their own radio it would be insane but a couple is not too bad. If it were to become distracting or a nusance, I would ban them completely. We are not in a patient traffic area but we do get a lot of outside visitors walking thru such as Resident's interviewing or clinicians. One must always consider patient care first and foremost and if music is offensive to you because of language then it probably is offensive to others as well. I think you should never have to endure music that is offensive in anyway. Jazz makes me crazy. My question to you would be, do you have a policy that stating that music is allowed? Best of luck to you. Christie > From: tracz...@aol.com > Date: Tue, 17 Jan 2012 21:37:04 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Music in the Laboratory > > Greetings. > I would like to know what other histology laboratories allow for music > players while working. Do you have formal policies about music content or > volume? Do you allow lab space doors to remain closed to muffle the volume of > what is being played? Are headsets allowed? > I am a terrible judge of this because I personally prefer to work in a > quiet environment. I am trying to be open minded, as long as the work gets > done. However, one of the techs had a song playing today that I believe was > inappropriate for general listening in the lab. Am I just out of touch? > Is that dang "F" word just something I'm going to have to learn to accept? > Do you have a written policy? When/how/why was it implemented? > I should mention that it's a small private lab, with minimal patient > traffic. We do see our share of FedEx, UPS, sales & service reps. > Your ideas on this is very much appreciated. > Dorothy > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Immunohistochemical staining of type IV collagen in Alport's Syndrome
We are looking into the feasability of doing this test in-house. Is anyone doing this test for kidney and if so, how did you run your validation? We have some positive patients but they are all skin so I guess we will need to purchase controls specifically for kidney. If anyone has any experience with this I would appreciate your comments. Thanks. Christie Gowan UAB University Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
I agree with you Jan. In my 30 + years as a histotech (yes, I am old too) I have seen floaters come from a variety of places but I am hard pressed to remember any floater coming from the water bath. Today we are blessed with DNA fingerprinting to determine if the floater is or is not from the patient but that still does not address the real issue of where did it come from and how do we stop it. The vendors stating that it is not an issue have never been re-biopsied because of a floater in with their tissue. Good discussion and long overdue. I look forward to the day when it is no longer an automatic response from all involved that it is "Histology's fault". Hope you are enjoying your new adventures in retirement. See you next year in Canada! > From: mamaw...@hotmail.com > To: histonet@lists.utsouthwestern.edu > Date: Wed, 5 Oct 2011 14:11:25 -1000 > Subject: [Histonet] (no subject) > > > Hello everyone,After being home from the NSH for a few weeks I have been > pondering an issue that I think bears discussion on the histonet.There have > been several papers published regarding "floaters" and the amount determined > to come from traditional staining buckets. There was also a poster presented > at the NSH this year on the subject.When I approached several vendors of H&E > stainers about this issue. The answers were surprisingly pretty much the > same. It is not an issue! Now I understand how one company can make this > claim as their stainer uses fresh stain on each slide. The explanations from > the other companies were insulting and just plain did not make sense to me. I > was told by a Histo tech vendor that "All Histo techs know that floaters come > from the water bath." Well, she was talking to a histo tech and I know for a > fact that floaters come from a variety of places. I have seen them from the > doctor's office or procedure room to the stainer and every step in between. > Sometimes if the "floater" is in the block it is very difficult to determine > where it originated. We can however eliminate the water bath and stainer as > the origin in these cases. One company told me that the design of the > solution bottle eliminated floaters because floaters float and their stainer > draws solutions from the bottom of the bottle. I have probably changed > thousands of staining dishes during my 40+ year career (yes, I am old) and I > have seen lots of little pieces of tissue at the bottom of the staining > dishes. So, no, not all floaters float. I would love to hear feedback from > others on this. I guess I would appreciate feedback about the floater issue > as well as how a few vendors can make such claims and expect Histology techs > to buy it. I really felt that a few comments were insulting to our profession > and to the knowledge and expertise we possess. JanOmaha > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job opening at Vanderbilt University Medical Center
Dear Netters, I am posting this for a friend who is not on Histonet. The following is a link to a job opening at Vanderbilt University Medical Center. Please respond to the link if interested. https://www.recruitingcenter.net/Clients/vanderbiltjobs/PublicJobs/controller.cfm?jbaction=JobProfile&job_id=30391 (ANOTHER LINK: http://www.jobtarget.com/link.cfm?c=SFrHQBOw83vA ) Thanks, Christie Gowan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re:2
Sorry to all my histonet friends. Someone hacked my email account and is sending out all these lovely little smam monsters. I have since addressed the issue and I hope it has stopped. Please let me know if you are still getting the little gifts and I will have to take more drastic measures. > From: tmallo...@hotmail.com > To: ejmal...@gmail.com; j...@rocketmail.com; e...@bestpiti.com; > xdgent...@sbcglobal.net; histonet@lists.utsouthwestern.edu > Date: Wed, 7 Sep 2011 05:44:54 -0400 > CC: > Subject: [Histonet] Re:2 > > ..Yeah! It’s really cool! This site is everything you need! > http://niftytrends.megabyet.net/friends.page.php?afriend_id=37og9 > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automatic H&E stainer with coverslipper (glass)
We currently use the Symphony from Ventana and the Leica with attached glass coverslipper. We are happy with both. We use the Leica to do research and the Symphony to do all routine clinical work. > Date: Wed, 31 Aug 2011 18:23:54 -0700 > From: ernestinemiddle...@yahoo.ca > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Automatic H&E stainer with coverslipper (glass) > > Hi; > Looking and need comments on those of you that are using combination H&E > stainer with glass coverslipper. > Thank you. > > Ernestine Middleton, Manager, HT, HTL ,BS ,MPA > Montefiore Medical Center > Bronx, New York > 718-920-4157 > emidd...@montefiore.org > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Specimen Collection Instructions
We have a website at our hospital that is accessible to all physicians and nursing staff for collection of all specimens. We call the website LabSource. > From: trathbo...@somerset-healthcare.com > To: mpe...@grhs.net; histonet@lists.utsouthwestern.edu > Date: Thu, 7 Jul 2011 19:36:15 + > CC: > Subject: [Histonet] RE: Specimen Collection Instructions > > We have the basic specimen requirements (taken from our procedure manuals) > from each of the Lab areas, and place them in a binder for each of the > Nursing units. So whether it is a fluid or a tissue specimen, or if it > requires special handling or collection, it is in this manual. If something > arrives collected improperly, the unit manager is notified and a specimen > incident report is generated. > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence > Sent: Thursday, July 07, 2011 2:55 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Specimen Collection Instructions > > How has everyone handled this question GEN.40104 Instructions are distributed > to physicians and paramedical personnel for proper collection, handling, > transportation, and preparation of cytologic and tissue specimens. > > I feel this question is asking me to spell out to a Dr how to collect his/her > specimens. To me this is like telling him/her how to practice medicine. > > I hope someone would like to share what you have seen that works. > > Thanks, Mike > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Request to help for rat eye histology
Are you fixing the eyes in Davidson's? We found that using this fixative which I would have to look up since it has been so long since I did rat eyes helped maintain better morphology. > From: himanshu.gu...@ucdenver.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 7 Jul 2011 11:37:39 -0600 > Subject: [Histonet] Request to help for rat eye histology > > Hi > > I need an advice from all experts there on histology of Rat eye. > > I need to take clear section of eye (cross section) so that I can see each > layer of cells clearly. Please advise me step by step process to prepare the > histology slides/sections of rat eye. > > I try it many times but I am not able to get the clear sections, the > cells/layers got broken. > > Regards > > himanshu > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Tissue Cassette Printer Recommendations
The Sakura and the Leica are the same system as they share a patent (this is what I am told). We use the Leica IPC for our cassettes and we have the Vantage system. We did a demo of the Thermo printer but were not happy with the speed and the fact that we would need to change who we order cassettes from. We did change the color of our routine cassettes from blue to white as the white scanned better at the vantage stations. We still use blue but only for autopsy cases. We use the Vantage system at the printer to scan in the patient bar code, choose a hopper with the color we want and hit print all. So far it has worked quite well. Christie Gowan cgo...@uabmc.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How many tissues an histo tech is suppose to cut per
The proper way to do this would be to time each tech in your lab as to how many blocks they can cut in one hour. Take everyones number of blocks and do an average for your lab. The average for my lab is 2.15 minutes per block. I compiled these numbers in an effort to determine what our labor costs are, not to set an expectation for productivity. I have some people who cut really fast and some who cut slow. I am curious as to how your management came up with these numbers and are any of them histotechs?? Christie > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne > Sent: Saturday, June 25, 2011 2:50 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] How many tissues an histo tech is suppose to cut per > > > > i've only been working 2 months. although older, i am new as a histotech > (graduated in may 2010, found a job in april 2011). seems management is > setting a goal of a block per minute as far as cutting goes for me. i have > until october to attain this goal. this minute for cutting is to include > facing, writing out slides, cutting, and putting tray into symphony stainer > (not to mention getting up to answer the phone, fielding questions regarding > send-out cases, and other slight "cutting interruptions). this seems an > extreme, possibly unattainable goal. i'm up for a challenge at age 53, but > any advice would be SWONDERFUL :) > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Confidentiality Notice: > This e-mail, including any attachments is the > property of Catholic Health East and is intended > for the sole use of the intended recipient(s). > It may contain information that is privileged and > confidential. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are > not the intended recipient, please delete this message, and > reply to the sender regarding the error in a separate email. > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Keeping Histo room floor clean?
We use a razor blade scraper but we turn it around and use the dull side. > Date: Wed, 15 Jun 2011 08:59:21 -0500 > From: sgoe...@mirnarx.com > To: histot...@imagesbyhopper.com; rosenfeld...@hotmail.com > Subject: RE: [Histonet] Keeping Histo room floor clean? > CC: histonet@lists.utsouthwestern.edu > > You can buy this type of thing too if you aren't the McGiver type. For > instance...from American Mastertech item # CPW04200E > > Sarah Goebel-Dysart, BA, HT(ASCP) > Histotechnologist > Mirna Therapeutics > 2150 Woodward Street > Suite 100 > Austin, Texas 78744 > (512)901-0900 ext. 6912 > > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > histot...@imagesbyhopper.com > Sent: Tuesday, June 14, 2011 7:50 PM > To: JR R > Cc: > Subject: Re: [Histonet] Keeping Histo room floor clean? > > We don't keep it off the floor, but do use a wide-bladed putty knife > attached to a mop handle to scrape the residual wax off the floor. It > woks quite nicely and doesn't remove the actual floor wax like a razor > blade scrapper would. > > Michelle > > Sent from my iPhone > > On Jun 14, 2011, at 6:44 PM, JR R wrote: > > > > > We keep getting a lot of paraffin on the floor of one histo > room--especially around the microtome and the embedding station. > > > > Short of laying down a tarp, what do folks do keep wax off of the > floor? > > > > Thanks, > > > > Jerry Ricks > > Research Scientist > > University of Washington > > Department of Pathology > > > ___ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANATOMIC Pathology
Sara, I have truly enjoyed this thread. Thanks for getting it started. Best of luck to you in the future. Christie Gowan HT(ASCP) Histology Supervisor Surgical Pathology Laboratories UAB Hospital (205) 934-4991 cgo...@uabmc.edu > Date: Tue, 14 Jun 2011 12:35:25 -0600 > From: sbree...@nmda.nmsu.edu > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] ANATOMIC Pathology > > With one exception (who shall remain nameless unless you want to send me > another $5.00...), the Consensus of Professional Opinion Including the > Samurai Pathologist (The Knower of All Things Histologic[al]) - I > christen thee ANATOMIC Pathology. And thank you, all, for allowing me > my leap across to the Land of Nonsensic[al]ness). I feel so much > better for having caused a ripple in the cosmic continuum. Without > further ado, I shall count my money and dream of that pathologist-less > island (sorry, guys). > > > > Sally Breeden, HT(ASCP) > > New Mexico Department of Agriculture > > Veterinary Diagnostic Services > > 1101 Camino de Salud NE > > Albuquerque, NM 87102 > > 505-383-9278 (Histology Lab) > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cost to produce one block
Hi Martha, She is getting different responses because institutions pay different amounts for their consumables depending on the quantity they use and any vendor agreements they may have. I broke down my cost by utilizing a years worth of purchasing information and number of blocks produced for that year. The information included all the consumables used to produce a paraffin block as well as labor. We actually broke down the cost for everything from grossing to glass slide for each CPT code. Of course one does not need to use a whole years worth of data but that is how I did it. I would be happy to help her if she wants to talk about it off line. Thanks. Christie Gowan UAB Hospital 205.934.4991 cgo...@uabmc.edu > From: mw...@wfubmc.edu > To: histonet@lists.utsouthwestern.edu > Date: Thu, 19 May 2011 19:26:47 + > Subject: [Histonet] cost to produce one block > > I am posting this question for a co-worker. She is attempting to calculate > the cost of producing one formalin-fixed, paraffin embedded block. She has > the amount of personnel time it takes but is having trouble with the > reagents, etc. While we know there are lots of variables she is wondering if > anyone would be willing to share this information with her. She is getting > differing numbers and is trying to figure out which amount is most correct. > Any responses will be kept confidential. > > Thanks! > > > Martha Ward, MT (ASCP) QIHC > Assistant Manager > Molecular Diagnostics Lab > Dept. of Pathology > Wake Forest University Baptist Medical Center > Winston-Salem, NC 27157 > 336-716-2104 > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dako Coverstainer System
Is anyone using this stainer and what are your thoughts? I have concerns about the reagents being so close to the ground. This stainer also locks you into purchasing Dako hematoxylin, bluing and eosin. Thanks. Christie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Histology Workflow Solutions
Hi Dana, I can't speak to the Elekta module but I have used the Vantage system for over a year now. We generate a bar code at accessioning that is used throughout the entire process to make cassettes, gross, embed, section, stain and turn out. We do not batch during any of the process other than the 20 slides we put in our staining tray. We scan each block at microtomy as we section which prints a label that we affix to the slide when we pick up the section. We have virtually eliminated errors at all stations. We were able to see quite an improvement in turn around times as well as tracking our specimens. This system also allows us to track quality issues anywhere in the process. Please feel free to contact me if you have further questions. Christie Gowan University of Alabama at Birmingham Surgical Pathology 205 934 4991 > Date: Mon, 28 Feb 2011 09:41:02 -0500 > From: dana.spen...@pcmh.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Histology Workflow Solutions > > I am currently investigating workflow solutions for our Histology Lab > incorporating bar code labeling. We use the PowerPath/ Elekta AP System. I am > looking into Elekta's AMP Module vs Ventana's Vantage system. I would welcome > any comments on these systems and how they work in your lab or if you use > something else. I would also welcome any suggestions or comments on how you > label slides...Do you batch print and scan barcodes of the blocks and slides > at the microtome? Do you scan blocks at the microtome and print labels there > for the slides? Do you scan blocks at the microtome and print slides at the > microtome? You may email directly if you prefer. Please share! Thanks in > advance for your help and feedback! > > Dana > > -- > The contents of this e-mail (and any attachments) are confidential, may be > privileged and may contain copyright material. You may only reproduce or > distribute material if you are expressly authorized by us to do so. If you > are not the intended recipient, any use, disclosure or copying of this email > (and any attachments) is unauthorized. If you have received this e-mail in > error, please notify the sender and immediately delete this e-mail and any > copies of it from your system. > == > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FW: What % of IHC Stains Should You Expect to Repeat?
Send the colon cancer control out and compare your results to the send out. If they are the same then you have an argument. > From: wanda.sm...@hcahealthcare.com > To: histonet@lists.utsouthwestern.edu > Date: Tue, 16 Nov 2010 13:07:28 -0600 > Subject: [Histonet] FW: What % of IHC Stains Should You Expect to Repeat? > > > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error and > that any use, dissemination, distribution, forwarding, printing, or copying > of this email or any attached files is strictly prohibited. If you have > received this email in error, please immediately purge it and all attachments > and notify the sender by reply email or contact the sender at the number > listed. > > > _ > From: Smith Wanda > Sent: Tuesday, November 16, 2010 1:59 PM > To: 'histonet-requ...@lists.utsouthwestern.edu' > Subject: What % of IHC Stains Should You Expect to Repeat? > > > Good Afternoon, > I am having an issue with my Pathologist who thinks when the patient tissue > on an IHC stain doesn't "look like what he thought it should look like", he > then wants to stop doing Immunos and send everything out This may happen > about twice a month, but the control always stains beautifully. We recently > had the stainer serviced and the drop zone was adjusted to alleviate any > problems with distribution of the solutions. On a recent test slide of a new > control block of colon cancer, he also didn't like that CD X2 did not stain > some of the tissue components, but the rest of the tissue on the test slide > stained beautifully. Please advise me on what to say to this Pathologist. > Thanks, > Wanda > > WANDA G. SMITH, HTL(ASCP)HT > Pathology Supervisor > TRIDENT MEDICAL CENTER > 9330 Medical Plaza Drive > Charleston, SC 29406 > 843-847-4586 > 843-847-4296 fax > > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error and > that any use, dissemination, distribution, forwarding, printing, or copying > of this email or any attached files is strictly prohibited. If you have > received this email in error, please immediately purge it and all attachments > and notify the sender by reply email or contact the sender at the number > listed. > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Leica ST5010/ST5020 vs Ventana Symphony
Hi Olivia, We currently use the Symphony stainer and based on our lab's needs, we love it. When choosing a stainer, you need to look at what your needs are. We stained so many racks of slides a day on our old Leica Autostainer that we were constantly changing solutions. Also, if we put more than 4 racks at a time on to stain, it bogged down the whole process. We used a separate oven to dry in so we were getting up to load the oven, getting up to load the stainer, getting up to change solutions and so we were making a lot of steps for one process. The Symphony is an enclosed system that contains 3 stainers, 2 ovens, one coverslipper and a bar code reader. When you purchase the system you are locked into their reagents so you need to see if this is cost effective for your lab. We were able to eliminate one position after purchasing the Symphony as well as our need to pay for waste disposal. I will say that we also use the vantage system in our lab and that has also impacted our eliminating the one position. We still use our Leica stainer and coverslipper for research and education and for a backup if the Symphony is down. They are both good stainers and each have their pros and cons. The other issue to think about is size and real estate. The Leica's are benchtop stainers and the Symphony is a stand alone which needs to be vented as well as distilled water flow. Hope this helps you a little. Christie Gowan UAB Medical Center Birmingham, AL > Date: Wed, 11 Aug 2010 12:58:32 -0700 > From: quaker...@gmail.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica ST5010/ST5020 vs Ventana Symphony > > For those of you who currently use or have used any of these instruments: > Why did you chose it and how do you like? If you've used both, which do you > like better and why or why not? > > We're currently manually testing. We're considering moving our lab into this > century and need a little advice. > > Thank you! > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Symphony
Kelly, We have had the Symphony since last October and I would be happy to answer any questions you might have. Our experience has been very good as a whole. Let me know what your specific questions are and I will be happy to answer them. > Date: Thu, 25 Mar 2010 06:05:25 -0700 > From: kdboydhi...@yahoo.com > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Symphony > > I was wondering who out there in Histo Land is using or has used the Ventana > Symphony H&E stainer and what their experiences have been. Thanks in advance!! > > > > Kelly D. Boyd, BS, HTL (ASCP) > Lab Manager > Harris Histology Services > > Tele (252)-830-6866 > (800)-284-0672 > Cell (252)-943-9527 > Fax (252)-830-0032 > > > > > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fire in the lab
Dear Histonet Friends, I just wanted to share an incident we recently had with an old paraffin pot. One of my techs came in on Sunday to embed some tissues, went into the processor room and smelled something burning. He noticed our old paraffin pot had charred looking labels on the outside so he went over, opened the lid and poof!!! the pot went up in flames. The thermostat had gone haywire and heated the paraffin to flash point. Opening the lid gave it the oxygen it needed to ignite. He triggered the alarm, made the appropriate call and then put it out with an extinguisher. Of course it kept re-igniting because he could not get behind it to pull the plug. The fire dept finally was able to get it pulled out and unplugged. Needless to say the tech was shaken and the room was a mess. I applaud his courage and am not sure I would have done the same. There was enough xylene and alcohol on the 4 processors to cause quite an explosion but everything else was in a flammable cabinet. I was wondering if this type of thing had ever happened to anyone else?? Needless to say, we have de-comissioned all old paraffin pots and will order only those with over temp safety features. I guess I just wanted to remind everyone that fires can happen in the lab and do probably more often than we hear about. This was the first time for me and I have been in this business for over 20 years. Take care and be safe. Christie Gowan HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microwave processors
Please include me on this as well. I have done quite a bit of research but am interested in what anyone has to say. Thanks, Christie > Date: Fri, 5 Feb 2010 16:09:43 -0800 > From: jeff.rin...@providence.org > To: Histonet@lists.utsouthwestern.edu > CC: > Subject: [Histonet] Microwave processors > > I am looking for information on the sakura microwave processor. We are > considering getting one, but before that I would like to hear some opinion on > the system(good or bad). I would also like to hear how it effected the work > flow . > Thank you > Jeff Lead HT (ASCP) > Sacred Heart Med Center > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Help! Losing sections from Superfrost Plus Slides
I would suggest you check the lot#. You may have received a bad lot and need to get them replaced by the vendor. >> > Hi all, > > We have suddenly started losing tissue sections from our Superfrost Plus > Slides. > Our students have been cutting fixed, frozen, cryosections (20um) and > thaw-mounting these onto Superfrost Plus slides. We have suddenly started > losing lots of sections from these slides (again!!). The tissue is small - > ie cross sections of frog aorta and longitudinal sections of nerves, so any > lose of adhesion results in total loss of the tissue. The odd thing is that > she is not losing every section on every slide, but half to 3/4 of the > sections are falling off within the first rinse. The sections are from the > same tissue block on the same slide while some falls and others don't. > > We are at a lose as to what we can do to rescue these sections. > > Does anyone know if there is any way to coat the slides in some solution with > the tissue on them to help improve the adhesion without losing the ability to > do immunofluorescence? > > Any further advice on cutting / drying protocols are welcomed. > This keeps happening and the inconsistency of it has us so frustrated with > this that we are thinking of going back to subbing our own slides. > > Thanks for your help! > > Angelina > > > -- ~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o > > Angelina Y. Fong, Ph.D. > Department of Zoology > Biological Sciences Building > 6270 University Boulevard > University of British Columbia > Vancouver, BC, V6T 1Z4 > Canada > Ph: (604) 822-5799 > Fax: (604) 822-2416 > Email: f...@zoology.ubc.ca > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
His skill set should be fine for any lab setting. I have worked in hospital labs and animal only private labs and had no problems. Christie > From: j...@opssearchgroup.com > To: histonet@lists.utsouthwestern.edu > Date: Thu, 3 Dec 2009 14:48:31 -0500 > Subject: [Histonet] (no subject) > > Histonetters, > > I am hoping you folks can provide an opinion. I am assisting an individual > who has a Biology degree, HTL certification and for the past 3 years worked > in the histology section of a private lab where they cut, embedded and > stained animal tissues. He would like to transition to a hospital lab. Is > there any reason his skill set and knowledge would be incompatible with his > desired new ambition? > > Jeri Vitello > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cryojane tap transfer system
Hi Richard, I am assuming that you are using the flash in the cryojane system to adhere the specimen to the slide. Make sure your tape is not too old and don't store it in the cryostat. It sounds like you are able to get the section but unable to get it to adhere. Make sure you pass your roller over the tape and slide several times before putting it under the flash and always use a positive charged slide from a freshly opened box. Good luck. I hope this helps a little Christie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Block Verification
You would send out for DNA fingerprinting. We have used the Cleveland Clinic for this. Christie > From: janice.maho...@alegent.org > To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu > Date: Mon, 24 Aug 2009 13:41:40 -0500 > Subject: RE: [Histonet] Block Verification > CC: > > Glen, > We had the same situation about a year ago. We sent the block and a bucal > smear out for DNA analysis. > Now we have the Vantage system to assure positive patient id. Check into it. > It saves time and worry and assures patient safety. > Jan > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen > Sent: Monday, August 24, 2009 1:05 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Block Verification > > All, > > Our histology lab would like to verify that 2 paraffin blocks are from the > same person to calm a pathologist's fears. Does anyone know of a good test > for this purpose...PCR of some sort perhaps?? Any suggestions would be > greatly appreciated. > > Thank-you In Advance, > > Glen Dawson BS, HT & QIHC (ASCP) > IHC Manager > Milwaukee, WI > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Sponsored by Catholic Health Initiatives and Immanuel Health Systems, Alegent > Health is faithful to the healing ministry of Jesus Christ, providing high > quality care for the body, mind and spirit of every person. > > The information contained in this communication, including attachments, is > confidential and private and intended only for the use of the addressees. > Unauthorized use, disclosure, distribution or copying is strictly prohibited > and may be unlawful. If you received this communication in error, please > inform us of the erroneous delivery by return e-mail message from your > computer. Additionally, although all attachments have been scanned at the > source for viruses, the recipient should check any attachments for the > presence of viruses before opening. Alegent Health accepts no liability for > any damage caused by any virus transmitted by this e-mail. Thank you for your > cooperation. > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Inactivation of DAB
Hi Peggy, You wrote: > We have started doing routine immunohistochemistry on our stainer and have a > large waste container that includes diaminobenzidine (DAB). > > We would like to inactivate it so we don't have to pay to have the waste > removed. Can anyone help me? What do people use to inactivate their DAB? May I suggest that you access the histonet archives where you will be able to see all the previous discussions on this topic. The responses were many and varied depending on different lab types and locations. Good luck with your search. Respectfully, Christie Gowan Surgical Pathology University of Alabama Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TMA tape sectioning system
The tape is made by instrumedics and the system is the cryojane tape transfer system. Christie > From: b-freder...@northwestern.edu > To: histonet@lists.utsouthwestern.edu > Date: Fri, 27 Mar 2009 13:39:06 -0500 > Subject: [Histonet] TMA tape sectioning system > > > > Does anyone out there remember who had the nifty sectioning tape for TMA's? > Or does anyone use it? The vendor was at the NSH meeting last fall. > > Thanks, > > Bernice > > > > Bernice Frederick HTL (ASCP) > > Northwestern University > > Pathology Core Facility > > ECOGPCO-RL > > 710 N Fairbanks Court > > Olson 8-421 > > Chicago,IL 60611 > > 312-503-3723 > > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] (no subject)
We run a third shift with 2 people and they do all the biopsies and stat specimens. This works great if you can keep the shift staffed. I have had to replace 5 people in 2 years. I am currently looking into getting a rapid processor so I do the biopsies during the day and totally eliminate my night shift. I would also like to add that the 3rd shift is not a very family friendly shift so it truly takes someone with special circumstances to stay with it for very long. Christie UAB Hospital Birmingham > Date: Thu, 5 Mar 2009 12:54:55 -0500 > From: aev...@wellspan.org > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > Does anyone run a third shift in their lab to relieve some stress on the 1st > shift crew?? I am looking in to running one person on third. I would like to > know how it is working for you, has it reduced costs, has it increased turn > around time...ect. Thanks!! > > > Andria B Evans, HTL(ASCP)CM > Anatomic Pathology > York Hospital > 1001 S. George Street > York, PA 17405 > 717-851-5006 > > "You can learn a lot more from listening than you can from talking. Find > someone with whom you don't agree in the slightest and ask them to explain > themselves at length. Then take a seat, shut your mouth, and don't argue > back. It's physically impossible to listen with your mouth open." -John Moe > > "Maturity is accepting imperfections." > > CONFIDENTIALITY NOTICE: > > This email may contain confidential health information that is legally > privileged. This information is intended for the use of the named > recipient(s). The authorized recipient of this information is prohibited from > disclosing this information to any party unless required to do so by law or > regulation and is required to destroy the information after its stated need > has been fulfilled. If you are not the intended recipient, you are hereby > notified that any disclosure, copying, distribution, or action taken in > reliance on the contents of this email is strictly prohibited. If you receive > this e-mail message in error, please notify the sender immediately to arrange > disposition of the information. > > > __ > This e-mail has been scanned by MCI Managed Email Content Service, using > Skeptic(tm) technology powered by MessageLabs. For more information on MCI's > Managed Email Content Service, visit http://www.mci.com. > __ > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] LabVision/Dako Autostainer
Hey Vinnie, We have 2 lab visions and we also experienced this problem. They came in and changed the level sensors and we have not had the problem since. Christie UAB Hospital Birmingham, AL> From: del...@musc.edu> To: histonet@lists.utsouthwestern.edu> Date: Fri, 23 Jan 2009 15:13:36 -0500> Subject: [Histonet] LabVision/Dako Autostainer > > We have earlier generation Dako Autostainers and are evaluating a current generation LabVision stainer. New or old we have experienced occasions when this instrument design has failed to dispense reagent. This seems to occur randomly or if there is a pattern we haven't figured it out. I've recently learned that some owners of the Biocare Nemesis which is essentially the same instrument have also encountered this problem.> > The arm will travel to the correct slide but nothing dispenses from the probe, then the arm moves on its way. > > I'd appreciate learning if others have experienced this problem and if a solution to the problem was discovered I hope you'll share it with me.> > > > Vinnie Della Speranza> Manager for Anatomic Pathology Services> Medical University of South Carolina> 165 Ashley Avenue Suite 309> Charleston, South Carolina 29425> Tel: (843) 792-6353> Fax: (843) 792-8974> > > > ___> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura tape problem
Sorry about the name. I meant Cheryl. TGIF __ From: "CHRISTIE GOWAN" <[EMAIL PROTECTED]> To: [EMAIL PROTECTED], [EMAIL PROTECTED] Subject: RE: [Histonet] Sakura tape problem Date: Fri, 21 Nov 2008 19:35:49 + > > >__ > > Cherly, > > I had this same problem a while back with an outside slide >that had > been sent to us for consultation. I even called the company to >get any >input from them. No luck. My final solution was to trim the tape >down >to just the area where the section was located. I sandwiched the >tape > between the glass slide and a glass coverslip using mounting >media > only. I used a paper clamp to secure it until it dried. Do not >use any > xylene. This will cause the tissue to start floating off the >tape and > scatter around. It can be pretty messy so don't use too much >mountant. > Good luck and let us know if you get any better solutions. > > Christie >___ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sakura tape problem
__ Cherly, I had this same problem a while back with an outside slide that had been sent to us for consultation. I even called the company to get any input from them. No luck. My final solution was to trim the tape down to just the area where the section was located. I sandwiched the tape between the glass slide and a glass coverslip using mounting media only. I used a paper clamp to secure it until it dried. Do not use any xylene. This will cause the tissue to start floating off the tape and scatter around. It can be pretty messy so don't use too much mountant. Good luck and let us know if you get any better solutions. Christie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CAP Question
I agree with Angela and this has always been ok for my CAP inspections. From: "Angela Bitting" <[EMAIL PROTECTED]> To: <[EMAIL PROTECTED]>, Subject: Re: [Histonet] CAP Question Date: Wed, 24 Sep 2008 15:49:58 -0400 I sign off on all of our daily maintenance/temperature charts at the end of each month. I interpret in this question that "instrument maintenance " means the daily or monthly maintenance that we do ourselves in the lab. Such as, changing solutions on our processors and stainers. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! >>> <[EMAIL PROTECTED]> 9/24/2008 3:26 PM >>> I am going through the CAP checklist and came upon this question: ANP23075:? Is there evidence of ongoing evaluation of results of instrumetn maintenance and function for all devices? The question before it asks if service reports are kept and they are.? How do I respond to this question other than the equipment works after being serviced!!!? Any ideas? Thank you, Ann ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. << AngelaBitting1.vcf >> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] NSH IHC Qualification Exam Class
Ethel Macrea taught the class at NSH and if you want the handout you can email her directly at [EMAIL PROTECTED] She no longer subscribes to the histonet so was not aware of these requests. From: "Evans, Andria B." <[EMAIL PROTECTED]> To: Subject: [Histonet] NSH IHC Qualification Exam Class Date: Tue, 23 Sep 2008 13:28:13 -0400 I was wondering if anyone could send me the handouts from the IHC Qualification class that was presented at the NSH Convention? I wanted to take that class, but ended up taking others. Please email me if you have them. Thanks! Andria B Evans, HTL(ASCP)CM CONFIDENTIALITY NOTICE: This email may contain confidential health information that is legally privileged. This information is intended for the use of the named recipient(s). The authorized recipient of this information is prohibited from disclosing this information to any party unless required to do so by law or regulation and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of this email is strictly prohibited. If you receive this e-mail message in error, please notify the sender immediately to arrange disposition of the information. __ This e-mail has been scanned by MCI Managed Email Content Service, using Skeptic(tm) technology powered by MessageLabs. For more information on MCI's Managed Email Content Service, visit http://www.mci.com. __ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
