[Histonet] Service provider for growing fungal strains?
Hello all! Does anyone know a lab that can grow specific fungal strains? We are looking to make internal FFPE control blocks for specific fungal strains. We have an internal method for producing FFPE blocks. Just need a lab or service provider that can grow specific fungal cultures and send us the fixed bugs. Any suggestions would super grateful! Best, Craig volle...@gmail.com cr...@champdx.com ᐧ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Suggestions on a used microtome
Hi everyone! Starting a new lab Texas and was looking at getting a used microtome and wanted to see if any of you had suggestions on the best model to get used and where? Any suggestion will be greatly appreciated! Best, Craig ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antigen retrieval survey
Hi, I am conducting a short 2 min survey for my science/business class examining current trends for antigen retrieval also known as heat induce epitope retrieval. Response will be greatly appreciated! https://www.surveymonkey.com/s/7989LKR Best, Craig Vollert Graduate Student Department of Pharmacological & Pharmaceutical Sciences SR2 521B College of Pharmacy University of Houston ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: AFB Controls
Hi Pamela, try contacting your local large animal vet or a veterinary diagnostic lab. Any case of Ovine or Bovine Johnne's disease would be a great ZN positive control. The bacteria (mycobacterium avium subsp paratuberculosis) is acid fast and congregates in huge numbers in the terminal ileum and ileo-caecal junction (and also the caudal mesenteric lymph node in smaller numbers). About the only problem i've ever come across is that there are so many AFBs it may mask poorly stained section unless you look for individual bacteria in the margins. Hope this helps Craig Craig Farish Senior TO Veterinary Diagnostic Laboratory Charles Sturt University Wagga Wagga NSW, Australia -- Message: 12 Date: Thu, 29 Sep 2011 18:23:07 + From: "Marcum, Pamela A" Subject: [Histonet] AFB Controls To: "histonet@lists.utsouthwestern.edu" Message-ID: <41d3a1af6fef0643bdc89e0516a6ea3209697...@mail2node2.ad.uams.edu> Content-Type: text/plain; charset="us-ascii" Does anyone know where we can get a good AFB control? Ours have exhausted and we can't find a case here with a positive reading. Best Regards, Pamela A Marcum UAMS Slot 502 4301 W Markham Street Little Rock AR 72205 Office: 501-686-7554 Fax: 501-686-7151 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.. Charles Sturt University | ALBURY-WODONGA | BATHURST | CANBERRA | DUBBO | GOULBURN | ONTARIO | ORANGE | SYDNEY | WAGGA WAGGA | Give Generously - Support Young Australians You can help young Australians to go to University and succeed in their studies by giving generously to the Charles Sturt University Foundation. To find out more or to make a donation go to the Foundation web site http://www.csu.edu.au/special/foundation. Australian donations are tax deductible. LEGAL NOTICE This email (and any attachment) is confidential and is intended for the use of the addressee(s) only. If you are not the intended recipient of this email, you must not copy, distribute, take any action in reliance on it or disclose it to anyone. Any confidentiality is not waived or lost by reason of mistaken delivery. Email should be checked for viruses and defects before opening. Charles Sturt University (CSU) does not accept liability for viruses or any consequence which arise as a result of this email transmission. Email communications with CSU may be subject to automated email filtering, which could result in the delay or deletion of a legitimate email before it is read at CSU. The views expressed in this email are not necessarily those of CSU. Charles Sturt University in Australia http://www.csu.edu.au The Chancellery, Panorama Avenue, Bathurst NSW Australia 2795 ABN: 83 878 708 551; CRICOS Provider Numbers: 5F (NSW), 01947G (VIC), 02960B (ACT) Charles Sturt University in Ontario http://www.charlessturt.ca 860 Harrington Court, Burlington Ontario Canada L7N 3N4 Registration: www.peqab.ca Consider the environment before printing this email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE CD79alpha dilemma - Thanks to all who replied
I've been given some good suggestions for both technique and clone changes. Thanks to all Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD79 alpha dilemma
Hi folks - has anyone out there found a Cd79α clone and supplier which will cover multiple species reliably? I can find clones which will stain cats and dogs (and humans), and clones which will cover horses, pigs, cows and primates (and humans, but not opossums for some reason??) but i'm yet to find one which will cross-react with both the large and small animals commonly encountered in a vet lab. I would rather not have to stock 2 versions of the same antibody if I can avoid it. Any suggestions would be much appreciated. As always, thanks to everyone who contributes to histonet, Craig Craig Farish Senior Technical Officer Veterinary Diagnostic Laboratory School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Please post JOB POSITION: MILWAUKEE WI
PURPOSE OF POSITION Perform a variety of routine and special histological and immunohistochemical staining procedures to support clinical and translational research activities in a new core lab at MCW. Develop and implements new techniques. ESSENTIAL DUTIES 1. Direct daily operations of all phases of histology and immunohistochemistry of the Clincal and Translational Research Core Lab 2. Train and supervise histotechnologists and core lab users 3. Develop, write, and validate histology and immunohistochemistry protocols 4. Develop and introduce new methodologies and techniques to support client users 5. Operate and maintain histology equipment, perform/supervise repairs, update computer hardware and software 6. Responsible for managing, maintaining, tracking, and stocking supplies 7. Assist with fiscal budgeting, capital equipment forecasting, purchasing 8. Responsible for safety issues regarding biohazardous materials and histology equipment 9. Participate in regularly scheduled meetings to evaluate data, methodologies, and procedures to establish and maintain a quality assurance program 10. Handle compliance issues 11. Responsible for meeting regulatory requirements (eg, CAP, CLIA 88) 12. Assist with outreach programs, grant writings, presentations, and manuscript preparations Contact: Dr. Craig Mackinnon, MD, PhD a.craig.mackin...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] TSH receptor
Thanks for the suggestion Bernice. I've been in contact with abcam (I maybe should have mentioned this). They have several TSH Receptor antibodies but unfortunately none are validated for IHC in sheep. They suggested a couple of possibilities based on cross-reactivity in pigs but obviously can make no definite recommendations. I though I'd see if anyone on histonet had any alternatives before I made any commitment. Cheers, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga NSW 2678 -Original Message- From: Bernice Frederick [mailto:b-freder...@northwestern.edu] Sent: Wednesday, 3 June 2009 10:59 PM To: Farish, Craig Subject: RE: [Histonet] TSH receptor Craig, Have you tried abcam? www.abcam.com. You can filter by reactivity in species. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Farish, Craig Sent: Tuesday, June 02, 2009 7:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TSH receptor Hi Folks, I'm looking for a TSH receptor antibody suitable for IHC in FFPE tissue from sheep. I'm struggling to find anything that may be applicable outside of the human field. Any suggestions would be most welcome. As always, thanks to all, Cheers, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga NSW Australia "I don't want to achieve immortality through my work. I want to achieve immortality through not dying" Woody Allen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TSH receptor
Hi Folks, I'm looking for a TSH receptor antibody suitable for IHC in FFPE tissue from sheep. I'm struggling to find anything that may be applicable outside of the human field. Any suggestions would be most welcome. As always, thanks to all, Cheers, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga NSW Australia "I don't want to achieve immortality through my work. I want to achieve immortality through not dying" Woody Allen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE Megacassettes
Hi Ian, Try Cellpath - www.cellpath.co.uk. It was cheaper for me to buy them from the UK and pay for shipping than it was to buy them in Australia. According to the catalogue they have a distributor in Cumbernauld called Surgical Supply Services but they're general number is 01686 611333. Good luck, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Boorooma Street Wagga Wagga NSW 2678 Australia "I don't want to achieve immortality through my work, I want to achieve immortality through not dying" Woody Allen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE Finding Iron in Decalcified Sections
Thanks to all who have offered me advice and ideas. I've taken it on board and I'll try a few new approaches in the coming weeks. I'll let you know the outcome. Cheers, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga, Australia c far...@csu.edu.au ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Finding iron in decalcified sections
Hi folks - I'd very much appreciate some help with this problem. I've been asked to performing the routine histo for a trial investigating the ability of chickens to sense direction. They are believed to have areas of iron rich tissue in their beaks which can detect the magnetic field of the earth in the same fashion as homing pigeons. The problem is this - to section the beaks I need to decalcify them, however I'm pretty sure that standard decalcification removes iron, at least partly. I know that the levels of iron in the tissue are very low and I've just trialled my technique on a pigeon with no success. I'm using a standard Perl stain (2% HCl + 2% K Ferrocyanide for 20 mins) on FFPE tissue which usually works very well for me, fixation is for a minimum of 24 hours in either 10%NBF or Bouins and decalcification is in either 5% Nitric acid or formic acid. Would anyone have any suggestions as to how to decal without removing iron or another stain which I could use in this situation? Thanks in advance for any and all suggestions, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga, Australia c far...@csu.edu.au ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE (Histonet) Bones
Hi Ian, Dermestid beetles are definitely the least smelly option (although they are not perfect). You'll find that there will usually be a residual smell in the short term. They can, however, damage small or delicate bones and the best way to deal with these is by bacterial maceration. This smells beyond belief. The anatomy dept here uses flow hoods but you can still smell it from the other side of the building. I've read about people putting them in tubs and leaving them outside(a very long way away) but that's probably not an option in the West End of Glasgow. When they are done, do not use bleach to whiten the bones as that makes them brittle - use dilute hydrogen peroxide (around 3 - 5%). It's worth trying some of the taxidermy forums as well - taxidermy.net is a good place to start. Have fun, Craig Craig (Joe) Farish Senior Technical Officer Veterinary Diagnostics School of Animal and Veterinary Sciences Charles Sturt University Wagga Wagga NSW 2678 Australia ' I don't want to achieve immortality through my work, I want to achieve immortality through not dying' - Woody Allen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
