Re: [Histonet] her 2 controls

[Dessoye, Michael via Histonet]

Check out Histocyte controls, which are available on slides or blocks.  One of 
their distributors is Cell Marque:

http://www.cellmarque.com/cms/histocyte/HER2.php

Their Her2 control include 0, 1+, 2+, and 3+.

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | mjdess...@commonwealthhealth.net | 
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
570-552-1484

-Original Message-
From: Nirmala Srishan [mailto:sris...@mail.holyname.org] 
Sent: Thursday, April 18, 2019 12:15 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] her 2 controls

Can someone suggest a company to purchase good Her 2 control slides. 
Preferably multi-tissue controls with +1,+2,+3.
Thanks in advance.


Nirmala Srishan
Supervisor Histology
Department of Pathology and Laboratory Medicine Holy Name Medical Center 
Teaneck, NJ
Office: 201 541 6328
Lab: 201 833 3023
sris...@holyname.org







Holy Name Medical Center is ranked among the top hospitals in the nation 
for patient care, clinical performance and workplace excellence.
Click here to learn more.

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[Histonet] ER/PR/Her2 control

[Dessoye, Michael via Histonet]

Hello Histonet!

Looking for some advice regarding ER/PR/Her2 controls.  I'm able to buy 
commercially TMA blocks containing varying levels of positivity for ER, PR, and 
Her2.  This work nicely because you can have a negative and varying levels of 
positive control as a same-slide control.  But our docs would like to score 
Ki-67 as well.  Currently, they are just doing pos/neg for Ki67.

It will take some time to collect cases and create a similar control, so I'm 
wondering what y'all are using?  Is there anything commercially available?  Or 
is everybody just collecting cases with varying degrees of positive?

Thanks,
Mike

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | 
mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484

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[Histonet] ER/PR/Her2 Controls

[Dessoye, Michael via Histonet]

Hello Histonet,

Can anyone recommend a commercial source of ER/PR/Her2 control tissue (array 
blocks would be preferred)?  I'm looking for something similar to the breast 
analyte control from Cell Marque, which is RUO:

http://www.cellmarque.com/cms/histocyte/breast-DR.php

Thanks,
Mike

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | 
mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484

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Re: [Histonet] glass slides

[Dessoye, Michael via Histonet]

That's interesting, we've been seeing warping with the Leica coverslips.  I 
wonder if there's something in common in their manufacturing.  We've been using 
a mixture of Superfrost Plus (Fisher), Basix (Fisher), and Platinum line 
(Mercedes Medical) all with good results.  The 'Basix' seems to be a good 
all-around non-charged slide that is affordable.  Hope this helps!

Mike

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | mjdess...@commonwealthhealth.net | 
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
570-552-1484


-Original Message-
From: Nancy Schmitt [mailto:nancy_schm...@pa-ucl.com] 
Sent: Monday, January 28, 2019 12:10 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] glass slides

Hello-
We are seeing some warping with glass slides from Leica.

Looking for recommendation on what you are currently using?

Thank you

Nancy Schmitt MLT, HT(ASCP)
Pathology Support Services Manager
United Clinical Laboratories


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[Histonet] Specimen disposal bags

[Dessoye, Michael via Histonet]

Hello Histonet,

I'm looking for some recommendations on biohazard bags for specimen disposal.  
Currently using Brighton 3.0 mil 44 gallon bags and they have occasional 
problems with leakage.  Obviously the formalin is removed but there is always 
some residual formalin present.  I'm willing to pay a little more for a better 
quality bag that is more leak-resistant.  Any advice?

Thanks,
Mike

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | 
mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484

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[Histonet] TMA slides for IHC validation

[Dessoye, Michael via Histonet]

I'm using TMA slides from US Biomax to assist with IHC validation.  I'm working 
on p120 on a Benchmark Ultra and BR2410 slides from Biomax.  The cases are all 
invasive lobular carcinoma, which should all stain positively, but only about 
half are staining positive.  I sent an identical slide to our reference lab and 
the staining matches my own.

Since it's the first time I've worked with TMA slides, am I missing something 
obvious?  Are the tissue cores generally prepared in the same fashion?  Any 
reasons why I'm seeing inconsistent staining of tissue that should all be 
positive?  All regular cases pulled of invasive lobular carcinoma are staining 
appropriately, just something unusual with these TMA slides.

Any advice is appreciated!  Thanks!

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | 
mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484

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Re: [Histonet] IHC validation

[Dessoye, Michael via Histonet]

Can anyone recommend a vendor that they've had good luck with for TMA slides?

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | mjdess...@commonwealthhealth.net | 
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
570-552-1484

-Original Message-
From: Terri Braud [mailto:tbr...@holyredeemer.com] 
Sent: Tuesday, March 20, 2018 1:54 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] IHC validation

Just another note:  You can order unstained tissue microarrays with the 
prerequisite number of cases, both positive and negative, and stain your 
validation all on one slide.  I've done this for years and for 3 different 
validations of entire IHC platform changes, ranging from 40 to over 100 
antibodies each time.  Saves time and money.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

Message: 2
Date: Fri, 16 Mar 2018 06:54:30 -0700
From: "Paula" 
Subject: [Histonet] Antibody Validation CLIA

Hello,
We've been discussing about the quantity of slides to run as a validation for 
IHC antibodies. We are governed by CLIA, and we would like to know if there is 
a set number of slides to run for a particular antibody we would like to bring 
in-house for Validation.  I think CAP requires 20 slides..?
And so we are asking if there is  a requirement with CLIA to run a certain 
number of slides, or is it up to us (the laboratory director) to decide how 
many slides to run for Validation/Verification.
Thank you in advance
Paula




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Re: [Histonet] ClearRite

[Dessoye, Michael via Histonet]

We've used ClearRite 3 and had no issues with IHC or molecular.

Michael J. Dessoye, M.S. | Histology/Toxicology/Special Chemistry Supervisor | 
Commonwealth Health Laboratory Services | mjdess...@commonwealthhealth.net | 
575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 
570-552-1484


-Original Message-
From: Cartun, Richard [mailto:richard.car...@hhchealth.org] 
Sent: Friday, June 09, 2017 1:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] ClearRite

Anyone using ClearRite (xylene replacement) for tissue processing?  If so, 
what's been your experience?  Any effect on IHC or molecular testing?  Thank 
you!

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] P16

[Dessoye, Michael via Histonet]

Ventana/Roche bought out MTM which had the patent, so they're the only vendor 
you can get it from.  Since you're using Bond you obviously don't want it in a 
dispenser, but you can buy their manual staining kit and just use the antibody 
from that.

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Commonwealth 
Health Laboratory Services | mjdess...@commonwealthhealth.net | 575 N. River 
Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1484


-Original Message-
From: Paula [mailto:plu...@biopath.org] 
Sent: Wednesday, June 08, 2016 12:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] P16

Hello,

Our pathologist would like us to get the P16 antibody. We have the Bond III and 
we use ready to use antibodies. Leica doesn't carry this antibody and so I was 
hoping someone can tell me which vendor carries it and that I can order it 
from.  

Thanks in advance for your help

Paula

Lab manager for BioPath Medical Group in California




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[Histonet] Air filter/purifier

[Dessoye, Michael via Histonet]

Hello Histonet,

Does anyone have experience with an air filter/purifier for use in the lab?  
I'm looking mainly for residual toluene fumes.  Everything is always below the 
limit but I have an employee who is unusually sensitive and I'm trying to help 
out.  I found one from Fisher for an astronomical price, and I don't know if 
there's anything that really makes it different from generic air purifiers you 
can purchase anywhere.  Does anyone use anything that they like?

Thanks,
Mike

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre 
General Hospital | An Affiliate of Commonwealth Health | 
mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486



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[Histonet] Peloris baskets

[Dessoye, Michael via Histonet]

Hello Histonet,

Does anyone know of any baskets (preferably spaced) that are compatible with 
the Leica Peloris?  I'm looking for alternatives to the astronomically-priced 
factory baskets.  Any thoughts?

Thanks,
Mike

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre 
General Hospital | An Affiliate of Commonwealth Health | 
mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486



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Re: [Histonet] Price gun

[Dessoye, Michael via Histonet]

Check this out: 
http://www.staples.com/Garvey-reg-1-Line-Pricemarker/product_69516

Lots of types available at Staples, Grainger, etc.  It will save you a lot of 
writing.

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre 
General Hospital | An Affiliate of Commonwealth Health | 
mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 
| Tel: 570-552-1432 | Fax: 570-552-1486


-Original Message-
From: Histology Technician [mailto:histology81...@att.net] 
Sent: Friday, January 08, 2016 9:43 AM
To: Histonet List; histonet-requ...@lists.utsouthwestern.edu
Subject: [Histonet] Price gun

  I was thinking of using a price gun to date my supplies when they come in, 
but I can't find one to buy.  Any ideas where to search for one?What do you 
guys use to date your supplies?  I've always used a pen to write the rcd date 
and my initials but thought a price gun would be quicker and neater :) Thanks!


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[Histonet] Cryostat vacuum

[Dessoye, Michael via Histonet]

Hello Histonet,

Does anyone know where I can find a cryostat vacuum?  I've used them in the 
past but can't seem to find a manufacturer.

Thanks!

Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre 
General Hospital | An Affiliate of Commonwealth Health | 
mjdess...@commonwealthhealth.net | 575 
N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1486



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