Re: [Histonet] mounting medium

[Diane Satterfield via Histonet]

We use Sub-X Mounting Medium from Leica.  This is what the pathologist wants us 
to use.

-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, November 13, 2017 5:47 PM
To: warda hassan
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] mounting medium

Hi,

This information would be very useful for me and probably others as well,
since I've just arbitrarily chosen one.
Can you summarize the responses you received for us?

Allan

On Wed, Nov 8, 2017 at 10:28 AM, warda hassan via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello to all
>
> Can anyone suggest which mouting medium is best that will help preservation
> of staining properties without creating fading of stains and bubbles on
> long run.
> Thanks alot
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[Histonet] Metal embedding molds-large

[Diane Satterfield via Histonet]

We are using large metal molds to embed mouse brains.  We are having a hard 
time getting to block out of the molds, the paraffin blocks are sticking.  
Sometimes they are coming out cracked.  Sometimes the cassette comes off the 
paraffin block.  Any idea why this is happening? Any advice on how to fix this 
problem?


Diane L. Satterfield, BS
Manager Brain Tumor BioRepository
Research Program Leader
Duke University Medical Center
Brain Tumor Center Biorepository and Database

diane.satterfi...@duke.edu
office  919-684-4642
pager  919-970-7328
fax  919-684-4975

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[Histonet] LFB-H&E controls

[Diane Satterfield via Histonet]

I am getting ready to learn how to do the LFB-H&E staining on brain tissue.  I 
was wondering want I would use for a control with this staining.

Diane
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[Histonet] H&E LFB

[Diane Satterfield via Histonet]

Does anyone have a protocol/SOP for doing H&E LFB staining they would be 
willing to share with me?


Diane L. Satterfield, BS
Manager Brain Tumor BioRepository
Research Program Leader
Duke University Medical Center
Brain Tumor Center Biorepository and Database
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[Histonet] Eosin used in processing

[Diane Satterfield via Histonet]

For those of you that use Eosin in the processing of tissue.  Can you please 
tell me what kind of Eosin mixture you use and how much?

Thanks
Diane
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[Histonet] Alcoholic Eosin-Phloxine in tissue processing

[Diane Satterfield via Histonet]

I am in the process of setting up a Leica TP1020 processor.  I was looking at 
someone else's SOP where in a 100% EtOH steps they add alcoholic eosin-phloxine.

Can someone explain to me what this does and do I have to add this to one of 
the 100% EtOH steps in the processing.

Thanks
Diane
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[Histonet] Leica TP1020 Processor question

[Diane Satterfield via Histonet]

I have acquired a Leica TP1020 tissue processor for my lab.  I have never had 
to process tissue before so I am reaching out for help.  I have no idea what 
protocol I need to be using. I am working with human brain tumor tissue that 
has been fixed in 10%NBF for 24-48 hours then transferred to 70% EtOH.  I need 
to know what to do with it from here.  Can someone help me with this please?  
Thanks.

Diane L. Satterfield, BS
Manager Brain Tumor BioRepository
Research Program Leader
Duke University Medical Center
Brain Tumor Center Biorepository and Database

diane.satterfi...@duke.edu
office  919-684-4642
pager  919-970-7328
fax  919-684-4975

CONFIDENTIALITY NOTICE:  The information contained in this electronic mail is 
sensitive, protected information intended only for the addressee(s).  Any other 
person, including anyone who believes he/she might have received it due to an 
addressing error, is requested to notify the sender immediately by return 
electronic mail, and to delete it without further reading or retention.  The 
information is not to be forwarded to or shared unless in compliance with Duke 
Medicine policies on confidentiality and/or with the approval of the sender.

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