[Histonet] RE: [IHCRG] AMACR (P504S)
We currently use Cat#Z2001, clone 13H4 from Zeta Corp with great results, although we only use it for HMWCK/p63/P504s stain. We have also used a predilute (PP200) from BioCare (that worked quite well). Sally From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Taylor, Jean Sent: Wednesday, February 23, 2011 9:41 AM To: histonet@lists.utsouthwestern.edu; ih...@googlegroups.com Subject: [IHCRG] AMACR (P504S) Hi Everyone, Our pathologists are interested in using this antibody to detect metastatic renal cell carcinoma in paraffin sections. They are not interested in an antibody cocktail, but also want to use it to detect prostate cancer. I'd like to know what company and clone most labs are using. Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- You received this message because you are subscribed to the Google Groups "ihcrg" group. The IHC Resource Group is a standing committee within the National Society for Histotechnology. To post to this group, send email to ih...@googlegroups.com To unsubscribe from this group, send email to ihcrg+unsubscr...@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en To contact the National Society for Histotechnology, email: hi...@nsh.org or call 443.535.4060. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD68
We also use KP1 Sally -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse Sent: Friday, April 30, 2010 10:49 AM To: 'Histonet' Subject: [Histonet] CD68 Happy Friday Everyone What clone is everyone using for the CD68 antibody for FFPE human tissue? Thanks for the info in advance. Everyone have a great weekend. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ER clone 1D5 or SP1 ?
We are using 6F11... Sally -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Friday, April 23, 2010 10:17 AM To: 'ih...@googlegroups.com'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ER clone 1D5 or SP1 ? I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Biocare Universal Negative Control Serum may be positive
We've been using that product for ages on our Ventana instruments and not had a problem. I wonder if it is batch related? We're expecting a new lot-guess we'll be taking an even closer look than usual at our testing slides! Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gagnon, Eric Sent: Wednesday, April 07, 2010 3:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Biocare Universal Negative Control Serum may be positive Having just validated and started using Biocare Universal Negative Control Serum, we were surprised when a pathologist brought back two cases in which tumour and/or epithelium came up positive. This staining was in the same locations as positive staining with some markers. We use Ventana BenchMark XT's, and the staining was in both iView and UltraView protocols. Mechanical problems, contamination and dilution of the Negative Control Serum did not appear to be factors. Biocare knows about this and is meeting with Ventana to iron out the problem. Would have been nice to know about this if it was a known issue before we bought and started using the product, but better late than never. Posting this in case anyone else on Histonet is experiencing similar staining challenges using this product. As Biocare states in the product insert sheet, when used in conjunction with non-Biocare kits, results may vary. We'll keep using it and are currently waiting for advice from Biocare. Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls
Yes, Linda and I select the harshest protocol in the list of antibodies we're running per case-we figure that the harshest will produce the biggest problems with background, etc. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 Hi Anita, We are currently examining the negative control issue in our laboratory. We also have Ventanas. We currently use a normal goat serum as our "non-immune" serum. We are considering changing our SOP's to use the Ventana Neg Control Rabbit and Neg Control Mouse in dispensers. Linda's suggestion of Biocare's Universal Negative Control Serum is intriguing. It would seem regressive to go back to the days of delineating mouse/rabbit antisera, using separate dispensers for each, and a universal negative seems to be the way to go, especially when using universal DAB kits. Using such a reagent would mean it could be applied to each case's negative control slide. Linda or others, would such a negative control serum protocol include your most aggressive pretreatment? Eric Gagnon MLT Histology Laboratory Kingston General Hospital Kingston, Ontario, Canada ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] P63
We use an IVD p63 (cat# CM163) from BioCare Medical. Contact me if you'd like further info. Sally Ann Drew, MT(ASCP) IHC/ISH Clinical & Research Laboratory UWHC 600 Highland Ave. DB1-223, Mail Code 3224 Madison, WI 53792 (608)265-6596 Fax:(608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Wednesday, February 03, 2010 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] P63 Do any of you Ventana users have a P63 that is not an RUO? If so, where do you get it from? Thanks a bunch. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC for renin?
We have a request for renin on a patient case. Is there a reference lab that does this? I've checked our usual send out places with no luck. Thank you for any info! Sally Ann Drew, MT (ASCP) sd...@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Need new ALK vendor
We are looking for a new anti-ALK antibody for formalin-fixed, paraffin-embedded tissue. If anyone would like to share their favorite source I'd certainly appreciate it. Hate to just throw a dart in the catalog cupboard, or spend lots of time getting information online without a nudge in any certain direction! Thank you! Sally Ann Drew, MT (ASCP) sd...@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] anti-H1N1 antibody?
One of our pathologists asked us whether we had heard on anyone having an antibody against H1N1 that could be used on formalin-fixed paraffin-embedded tissue. Anyone doing this? Or working on it? Thanks for any and all responses. Sally Ann Drew, MT (ASCP) sd...@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HNF-1
One of our pathologists has inquiring whether there a reference lab that offers the antibody HNF-1B? Thanks for any thumbs-up! Sally Ann Drew, MT (ASCP) sd...@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Beta Amyloid
My co-worker previously asked about any versions of this antibody that didn't require formic acid pretreatment. I was wondering if there is experience out there using the antibody from DBS, and what the consensus is about the stability of the antibody (from any company) after being diluted. We currently make it up the day of use, but in anticipating an increased volume were wondering about making up larger volumes "in advance." Thank you for your thoughts and time. Sally Ann Drew, MT (ASCP) sd...@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC control debate
I think that besides checking Histonet archives for past discussions on this, if you keep a file of freshly cut, freshly stained control slides that you can go back to compare day-to-day results with, you will be able to pinpoint fading stains better. You may also want to have cut control slides inventory that coincides with an estimated workload-for example, have 1 months-worth of tonsil at room-temp and 2-3 months in a frost-free freezer. If you have some at both storage conditions, then you can check the staining quality versus the control section's age as you optimize antibodies. Although this is a generalization-most of the stains we've seen affected by control age/storage conditions have been nuclear-staining antibodies. Sally Ann Drew, MT (ASCP) sd...@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lynette Pavelich Sent: Tuesday, May 26, 2009 1:16 PM To: histonet@lists.utsouthwestern.edu; f...@ufl.edu Subject: [Histonet] IHC control debate Hello, I need help from all of our IHC expert guru's! We have a debate going on about the known positive control blocks. All are FFPE with 8-48 hrs in formalin. All have been tested and have been approved for use as positive controls. We currently cut the positive control from the block as needed. I have learned that some controls do start to deteriorate over time, tonsils for example. But, is there a list, an article, SOMETHING that can guide me so that we can cut some of these controls ahead of time? This would not only save tech time, but would further the use of our controls. In cutting the block, some of the good control is wasted and some positives are hard to find. My argument is that the purchased controls work wonderfully, even after a very long time and our improved detection kits have improved so much over the years, it makes this possible. If there is no published article out there, I implore you experts to please help us in this area. Would also like to hear from the technical experts from our suppliers too! Until then, the debate continues on!!! Thank you!! Lynette ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] INI1 antibody?
Is anyone aware of a reference lab that has INI1 antibody one their menu? An inquiring neuropathologist wants to know... All positive responses will be happily forwarded to him... Thank you! Sally Ann Drew, MT (ASCP) sd...@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hgb A antibody
Anyone have a favorite anti-Hgb A antibody vendor? We're considering bringing this into our inventory of antibodies for formalin-fixed, paraffin-embedded tissue, but of the catalogs(some rather old) we have here I have only found one vendor... Thanks for any opinions on this subject. Sally Ann Drew, MT (ASCP) sd...@uwhealth.org IHC/ISH Lab DB1-223, Mail Code 3224 600 Highland Ave. Madison, WI 53792 Phone (608) 265-6596 Fax (608) 262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
