[Histonet] Bachelor's degrees to sit for HTL

[Fawn Bomar via Histonet]

Hi everyone,



I was wondering if anyone would be able to help my co-worker and myself in 
trying to figure out exactly what we need to do to obtain a bachelor's degree 
that would qualify us to sit for the HTL.  As of right now we both have 
associate's degrees and our HT certification.  We both work full time and have 
children so we cannot attend college full time and would have to do most of it 
online if possible due to our location in South Boston, Virginia.



We are trying to figure out how to blend online classes with the science 
classes that may have labs required that we would have to attend campus for.



Another question we are having is what specific bachelor's degree do we have to 
obtain, does it have to be a biology degree or a science degree?



Thanks for all your help,



Fawn Bomar
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[Histonet] Disposal of Processor

[Fawn Bomar via Histonet]

Does anyone know if you need to decontaminate a tissue processor in any special 
way to dispose of it?  If the tissue has all been fixed before being in the 
processor and there were no CJD cases processed on it, is it considered 
biohazardous?



Thank you

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[Histonet] Decon Processor

[Fawn Bomar via Histonet]

Hi everyone,



Our lab is getting ready to install a new processor.  My question is, how do we 
go about decontaminating our old processor to dispose of it.  The old processor 
is a Ventana Rennaissance.



Thank you

Fawn Bomar
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[Histonet] Professiional competency

[Fawn Bomar via Histonet]

Does anyone have a policy for assessing professional competency and a log for 
recording the assessment for pathologists who provide interpretive services to 
the lab that they would be willing to share?



Thank you

Fawn
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[Histonet] Lead Tech/ Supervisor Competency

[Fawn Bomar via Histonet]

Hi all,



I didn't receive any responses in regards to my last post so I thought I would 
try one more time.  Does anyone have a competency or orientation checklist that 
they use for lead techs or supervisors that they would be willing to share?  On 
my last post, everyone that messaged asked for copies of my assessment and the 
assessments that were sent to me, which I did send to everyone that requested 
them.  If anyone else would like me to send my assessment to the them to look 
at, please let me know and I will be happy to share, plus I would be 
appreciative of any feedback- positive or negative- and any suggestions for 
improvement.  I tried to send an attachment of my assessment to the histonet 
but it kept saying it was waiting for moderator approval and it never was sent.



Thank you all for your help and guidance.



Fawn
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[Histonet] Competency

[Fawn Bomar via Histonet]

Hi to everyone yet once again!



I am sorry to bother you all with yet another question, but I was wondering if 
anyone had a competency/orientation checklist specifically for team leaders/ 
Supervisors.



I received many responses and great competency checklists from the group for 
the histotech position itself and managed to make one that fit our lab using 
tips from all of them.  I can't thank you all enough for all your help and for 
sharing all your knowledge with everyone!



Thank you again,



Fawn
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[Histonet] Temperatures

[Fawn Bomar via Histonet]

Hi All,



I was wondering how everyone tracked their cryostat and paraffin temperatures.  
Do you all just use the machine temperature, or do you all use the machine and 
a second thermometer as verification?



Thank you

Fawn
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[Histonet] Help on Competency Assessments

[Fawn Bomar via Histonet]

Hey everyone,



I know that this question has been asked before and I have tried to find out 
information through the internet, but I am having a difficult time trying to 
find any type of sample forms.  Would anyone be willing to share their 
competency assessment forms/checklists?  I am trying to make one up for our lab 
and different areas and need some guidance to ensure that I cover everything 
that needs to be assessed.  I know that I need to have the collection and 
transporting of specimens from the OR/Endo areas, receiving/accessioning and 
labeling specimens/blocks/slides.  Preparing/sectioning/staining Frozens.  
Embedding/sectioning/staining routine stains/special stains/IHC stains.  
Documenting/performing of equipment maintenance/function 
checks/decons/temperature checks.  Preparing and labeling cytology specimens 
received in lab/ prepared FNA slides/specimens from FNA's/ Prepared 
slides/specimens from CT guided procedures.



I am having a hard time trying to figure out exactly how to document and which 
of the six (if not all) competency elements are needed to be checked for each 
of the areas.



If anyone is willing to share their templates (and how they actually document, 
i.e., initial, checkmark)  or perhaps point me to a website, book, or person to 
reference, I would be very grateful!!!



Thank you,



Fawn Bomar
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[Histonet] Morgue Refridgerator Temperature

[Fawn Bomar via Histonet]

Does anyone have a procedure for recording their morgue refridgerator 
temperatures that they would be willing to share?  We do not perform autopsies 
at our facility any more but we have the refridgerator that the bodies are 
temporarily stored until they are picked up.  We are monitoring the temperature 
but do not have an actual procedure written.  I was wondering about a few key 
points such as,



1.  What is the temperature range

2.  Do you have a specific time allocated to check the temperature

3.  If a body is placed in the fridge, do you give it a certain amount of time 
to get the temperature back into range

4.  Do you have the other departments fill out a log of some sort to know if 
and when they place bodies in the fridge to account for any temperature changes



Thank you

Fawn Bomar
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[Histonet] Stainer

[Fawn Bomar via Histonet]

Thank you everyone for your input!  So far most people like the Sakura, the 
hard part is figuring out where to fit it.



Thank you

Fawn
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[Histonet] H&E stainers

[Fawn Bomar via Histonet]

Hi everyone,



I know this question has been asked before but I am having difficulty finding 
it.  We are in the process of finding a new H&E stainer and was wondering what 
everyone would recommend or not recommend.  We are a small facility- stain less 
than 100 H&E slides a day.  We are also looking for one that is compatible with 
a coverslipper.  Right now my manager is looking at the Sakura Prisma but we 
would have to do some major reconstruction to fit it in our lab.  We are also 
looking at the Gemini with the Clearvue coverslipper.  Can anyone give me their 
opinions on what they think?



Thank you

Fawn
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[Histonet] ER/PR Benchmarks

[Fawn Bomar]

   





= 


   


   Hi all,
   


   &n= bsp;
   


   Does  anyone  know  where to find the ER/PR benchmark numbers= that we
   are to use to assess our annual numbers?
   



   
= 


   Thank you
   


   Fawn
   


   


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[Histonet] Looking for an article

[Fawn Bomar]

Hi all,



I need some help in locating an article for class, preferably one I don't have 
to pay for.  The name of the article is "The Effectiveness of Honey as a 
Substitute for Formalin in the Histological Fixation of Tissue."



The only place I found this article so far charges $48 dollars for 24 hour 
access and I need to read it and write a summary for my class so I need longer 
than 1 day.



Thank you for any help



Fawn
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[Histonet] Storage of paraffin blocks

[Fawn Bomar]

Hi everyone,



I hope all is going well in the new year.  I was wondering if everyone that 
stores their paraffin blocks in the plastic tissue tek block filing cabinets, 
stores these cabinets directly on the floor or do you all place them on some 
sort of stand?



Thank you

Fawn
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[Histonet] fixation question

[Fawn Bomar]

What is the minimum time recommended for fixing routine endoscopy biopsies 
before placing them on a short bx run program.



Thank you for your help



Fawn
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[Histonet] Ventana Symphony

[Fawn Bomar]

Can anyone using the Ventana Symphony share its pros and cons with me?  Would 
you recommend this stainer?



Thank you

Fawn
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[Histonet] possible TB case

[Fawn Bomar]

Hey histonetters!!



We just performed a frozen section on a lymph node that looks like it may have 
TB-  I know we will have to decontaminate the cryostat but what else do I have 
to decontaminate?  Will I have to do anything special to the processor or 
microtome?



Thank you in advance



Fawn
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[Histonet] Tracking control blocks

[Fawn Bomar]

Happy Easter everyone!!!  I was wondering if anyone had a tracking system that 
they would be willing to share in regards to how you all are keeping a record 
of your control blocks for IHC.  As of right now, we are cutting a slide and 
staining it for the pathologist to approve- then keeping the paperwork and 
slide in files, and putting a label on the block in use with the date of 
approval.



Thank you

Fawn
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[Histonet] Frozen section TAT

[Fawn Bomar]

Hi Everyone,



Hope you all had a wonderful Christmas and a Happy New Year!!



I am working on my Quality Indicators/Assessment and was setting a goal for our 
frozen section TAT's and was wondering if there was an overall standard TAT 
goal that everyone was using as far as percentage of frozens reported within 20 
minutes. (i.e., goal of less than 1% outside of 20 minutes or goal of 90% being 
completed within 20 minutes).  Also, do you all record the time that you give 
the pathologist the slide as the end time or the time the pathologist reports 
the results as the end time?



Thank you in advance

Fawn
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[Histonet] Old equipment

[Fawn Bomar]

Would anyone be interested in purchasing some old equipment?  We have 2 Ventana 
Benchmarks that will be available in late November/ early December and a Leica 
RM 2155 microtome.  The Benchmarks work, my company just upgraded to the 
Benchmark XT.  The Leica microtome was having some issues but we couldn't get 
service on it so we bought a new one.



Thank you

Fawn Bomar
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[Histonet] Validating on new Benchmark

[Fawn Bomar]

Hi everyone,



I have a few more questions that I was hoping I could get some help on.



1.   How did everyone go about obtaining the positive and negative 
controls-(did you choose patient cases or go with generic controls such as 
extra tonsil, appendix, colon,etc...)



2.  For the negative controls do you have to find separate negatives or can you 
use the internal negative controls if the tissue has it.



3.  Do you have to do a minimum of 10 positive and negatives or can we do 5 
(other than the ER/PR and Her2)



Thank you

Fawn
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[Histonet] Validating new Benchmark instrument

[Fawn Bomar]

Hi everyone,



I know that this subject has come up several times and I am sorry to bother 
everyone again but I was hoping to find some help.  We are upgrading our 
Benchmark Immuno machines to the Benchmark XT immuno machine at the end of 
October.  Does anyone have any suggestions on how to approach the validation 
process?  Do we need to validate every antibody that we use as well as the 
kits?  I know that there is also a gray area surrounding how many slides need 
to be run to consider the antibody validated, but what would be the suggested 
amount of slides to validate a new machine as I know this will be very costly.  
Does anybody have a log/checklist/spreadsheet that they would be willing to 
share to help document this validation of the machine and each of the 
antibodies?



Thank you in advance for your help



Fawn Bomar
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[Histonet] Microtome Service

[Fawn Bomar]

Does anyone know of any company that is able to service the Leica RM2155 
microtomes?  We are located in South Boston, Va.



Thank You



Fawn Bomar
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[Histonet] PIN4 antibody

[Fawn Bomar]

Hi everyone,



Has anybody used the PIN4 cocktail using the Venatan Benchmark stainers?  If 
so, which company do you purchase it from and would you be willing to share 
your protocol?



Thanks



Fawn Bomar
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[Histonet] RE: cassettes

[Fawn Bomar]

We haven't been able to get any either.


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Gale Limron 
[ga...@unionhospital.org]
Sent: Tuesday, April 02, 2013 9:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] cassettes

Has anyone else had trouble getting white Tissue-Loc cassettes (Histoscreen), 
or any other color? Ours have been on backorder for almost two months from our 
supplier and they say it could be at least another month until they are 
released. We're told it is a manufacturing delay...
Thanks,
Gale

Gale Limron CT,HT (ASCP)
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext 2562





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[Histonet] Control Block Log Sheets

[Fawn Bomar]

Does anyone have a template they would be willing to share on how to keep track 
of control blocks?  Do you all use a type of log sheet to keep track, do you 
all number them a certain way to keep track?



Thank you

Fawn
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[Histonet] Process Improvements

[Fawn Bomar]

Hi everyone,



My new manager would like me to come up with a list of process/performance 
improvements in the pathology department that can be measured on a 
monthly/quarterly basis, such as they do for other departments in the hospital, 
i.e. the ER measures the number of patient falls with <5 per quarter being 
their goal.  I need advice on what to measure for our 
histology/pathology/cytology departments.  Does anyone have any suggestions?



Thank you

Fawn
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[Histonet] Annual Competency Checklists

[Fawn Bomar]

Hello Everyone!



Does anybody have an annual competency checklist for their employees that they 
are willing to share?



Thank you

Fawn
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[Histonet] CLIA Inspection

[Fawn Bomar]

Hi everyone!



I was wondering if anyone out there that has knowledge of CLIA regs and 
inspections would be willing to contact me directly to help me answer a few 
questions.



My contact info is 
fawn.bo...@halifaxregional.com or call 
if it is easier at 434-517-3033.



Thank you in advance,

Fawn
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RE: [Histonet] IHC for H. pylori

[Fawn Bomar]

We run giemsa's on all stomach bx's


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Subject: Re: [Histonet] IHC for H. pylori

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[Histonet] Thyroid Smears

[Fawn Bomar]

Hello Everyone!

Happy New Years to all!  I have a question regarding the preparation of thyroid 
smears.  As of right now, we go up to the room and collect the thyroid sample.  
The Pathologist makes the smears in the room and immediately puts them into 95% 
Isopropanol to fix.  We then complete the stain later on in the day.  The 
problem that we are encountering is that all of the blood and cells are coming 
off of the slides before we make it through the entire stain.  Does anyone have 
any suggestions or are willing to share the procedure that they use?  We had a 
couple of suggestions that we recommended to the Doctor but they were 
dismissed. I don't want to tell what are suggestions were so that the doctor 
cannot accuse us of influencing every one else's opinions.

Thank you in advance,
Fawn
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[Histonet] RE: QA Questions

[Fawn Bomar]

1.  We don't count folds either.
2.  Complains mainly about immunos 
3.  First slides come out about 8:30 and all are out by 10:30
4.  If the IHC's are ordered by 11:30pm then we do same day turn around


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce 
[jwe...@sjha.org]
Sent: Monday, September 27, 2010 11:02 AM
To: Clare Thornton; 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: QA Questions

1. We don't count folds.
2. Quality of special stains and immunos.
3. First slides are ready by 8:00 and all out by 11:00.
4. IHCs ordered by noon are out same day.


Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton
Sent: Monday, September 27, 2010 09:57
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] QA Questions

I would love to hear some other labs' responses to these questions.  For 
clarification, we are a private clinical lab, and we average about 50,000 
cases/year.


1.  What percentage of slides to you have that have greater than 1 fold?

2.  On average what do your pathologists complain about as far as quality?

3.  What is your Turn Around Time?   What time do the pathologists start 
getting slides and what time do they have all of their work for the day?

4.  What is your IHC turn around time?  If the pathologists order IHC tests 
before what time do they have same day turn around time?


Clare J.Thornton, HTL (ASCP), QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthorn...@dahlchase.com


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RE: [Histonet] HER 2

[Fawn Bomar]

I-VIEW DAB Detection Kit from Ventena


From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sara Baldwin/mhhcc.org 
[sbald...@mhhcc.org]
Sent: Wednesday, September 22, 2010 3:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HER 2

Which detection kit do you use?

Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbald...@mhhcc.org
Ph 812-482-0210, 482-0216,  Fax 812-482-0232,
Pager 812-481-0897
Confidential information, Authorized use only.
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[Histonet] Her2 question

[Fawn Bomar]

Hi everyone,

I was wondering how many of you all use the Cell Marque Her2 predilute?  We 
have been using it at our facility for 2 years now and it has been great until 
we received a new lot.  This new lot does not seem to be staining as well and I 
was wondering if anyone else was having the same problem and if they managed to 
get it to stain better.  I called Cell Marque and they told me that they 
optimized the antibody again so it is different from what we have been using.  
I am adjusting a few things on our protocol to see if it works and I was 
wondering if anyone had any tips.

We use the BenchMark LT and our protocol is as follows:

Deparaffinization, Standard CC1, Antibody incubation 32 minutes (the maximum 
that the machine allows), A/B Block, Hematoxylin-6 minutes, and bluing-4 
minutes.

Thank you
Fawn Bomar HT(ASCP)
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[Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17

[Fawn Bomar]

One more thing This was my reply to Lynn and her response to my question.

Fawn


From: Fawn Bomar
Sent: Monday, February 15, 2010 9:24 AM
To: Jones, Lynne
Subject: RE: FYI - Histonet Digest, Vol 75, Issue 17

Hi Lynne,

My inquiry about the Her2 validation was to get overall suggestions on how 
different labs interpreted how to go about the validation process.  I said that 
we had a process but since I was new to the clinical aspect and to immuno's in 
general, I wanted to make sure that we were up to par with everyone else or if 
I needed to make adjustments to make our validation better.  I was making sure 
that we met or exceeded expectations and I felt that everyone could offer an 
opinion.  There should be no doubt in confidence in our accreditation...there 
should be some comfort in knowing that I am making sure that we are doing the 
best work that can be done.

Sincerely
Fawn

From: Jones, Lynne [jone...@mir.wustl.edu]
Sent: Friday, February 12, 2010 5:48 PM
To: Fawn Bomar
Subject: FYI - Histonet Digest, Vol 75, Issue 17

Hello -
I was curious about the facility where you worked, so I looked up Halifax 
Regional, but it seems congratulations are in order, since Gwen Wade just 
posted a Press release indicating that the lab passed CAP accreditation.  
(Though I'm not sure your inquiry about a fundamental process in a histology 
lab would inspire confidence.)

HistoNet is a fabulous resource, but there are some questions you may want to 
ask using a less traceable e-mail account.  HistoNet archives are readily 
accessible, even to the general public.  I do understand the panic and 
second-guessing that hits right before the inspectors appear, but you obviously 
had all the I's dotted and t's crossed.  The next time will be easier. :)

Take care,
Lynne




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-
--

Message: 3
Date: Thu, 11 Feb 2010 14:06:56 -0500
From: "Fawn Bomar" 
Subject: [Histonet] Help...
To: "histonet@lists.utsouthwestern.edu"

Message-ID:

Content-Type: text/plain; charset=iso-8859-1

Does anyone have a protocol or procedure for validating the Her2 antibody?  We 
are trying to get ready for a CAP inspection and I'm not sure if what we did is 
ok or not.

Thank you in advance
Fawn
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Thank you


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Message: 4
Date: Thu, 11 Feb 2010 12:28:27 -0700
From: "Liz Chlipala" 
Subject: RE: [Histonet] Help...
To: "Fawn Bomar" ,

Message-ID:

Content-Type: text/plain;   charset="us-ascii"

Fawn

CAP has specific quidelines, its right there in the questions, did you
do what that question is asking and have you documented what was done.
They tell you right in the check sheet what needs to be completed.  Test
validation must be performed on a minimum of 25 cases (they recommend
25-  100) and they tell you how to validate too.  There is also an
article that you can get on the CAP website on IHC standardization. I
have a pdf of it here if you need it.

LIz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com


Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn
Bomar
Sent: Thursday, February 11, 2010 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help...

Does anyone have a protocol or procedure for validating the Her2
antibody?  We are trying to get ready for a CAP inspection and I'm not
sure if what we did is ok or not.

Thank you in advance
Fawn
-
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for the use of the individual(s) and entity named as recipients
in the message.

If you are not an intended recipient of this message, please
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do not disclose its co

[Histonet] FW: FYI - Histonet Digest, Vol 75, Issue 17

[Fawn Bomar]

Hi everyone,  

 Since I received this e-mail from Lynne about a question that I asked, I 
decided it was probably in my best interest not to ask any more questions in 
general.  Thank you to everyone that helped verify that my validation process 
was indeed up to par!  I will stay on the histonet in hopes that I can help 
others but I will keep my own questions to myself

Fawn


From: Jones, Lynne [jone...@mir.wustl.edu]
Sent: Friday, February 12, 2010 5:48 PM
To: Fawn Bomar
Subject: FYI - Histonet Digest, Vol 75, Issue 17

Hello -
I was curious about the facility where you worked, so I looked up Halifax 
Regional, but it seems congratulations are in order, since Gwen Wade just 
posted a Press release indicating that the lab passed CAP accreditation.  
(Though I'm not sure your inquiry about a fundamental process in a histology 
lab would inspire confidence.)

HistoNet is a fabulous resource, but there are some questions you may want to 
ask using a less traceable e-mail account.  HistoNet archives are readily 
accessible, even to the general public.  I do understand the panic and 
second-guessing that hits right before the inspectors appear, but you obviously 
had all the I's dotted and t's crossed.  The next time will be easier. :)

Take care,
Lynne




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-
--

Message: 3
Date: Thu, 11 Feb 2010 14:06:56 -0500
From: "Fawn Bomar" 
Subject: [Histonet] Help...
To: "histonet@lists.utsouthwestern.edu"

Message-ID:

Content-Type: text/plain; charset=iso-8859-1

Does anyone have a protocol or procedure for validating the Her2 antibody?  We 
are trying to get ready for a CAP inspection and I'm not sure if what we did is 
ok or not.

Thank you in advance
Fawn
-
This electronic message may contain information that is
confidential or legally privileged.  It is intended only
for the use of the individual(s) and entity named as recipients
in the message.

If you are not an intended recipient of this message, please
notify the sender immediately and delete the material from any
computer. Do not deliver, distribute, or copy this message, and
do not disclose its contents or take any action in reliance on
the information it contains.

Thank you


--

Message: 4
Date: Thu, 11 Feb 2010 12:28:27 -0700
From: "Liz Chlipala" 
Subject: RE: [Histonet] Help...
To: "Fawn Bomar" ,

Message-ID:

Content-Type: text/plain;   charset="us-ascii"

Fawn

CAP has specific quidelines, its right there in the questions, did you
do what that question is asking and have you documented what was done.
They tell you right in the check sheet what needs to be completed.  Test
validation must be performed on a minimum of 25 cases (they recommend
25-  100) and they tell you how to validate too.  There is also an
article that you can get on the CAP website on IHC standardization. I
have a pdf of it here if you need it.

LIz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com


Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn
Bomar
Sent: Thursday, February 11, 2010 12:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help...

Does anyone have a protocol or procedure for validating the Her2
antibody?  We are trying to get ready for a CAP inspection and I'm not
sure if what we did is ok or not.

Thank you in advance
Fawn
-
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The material in this message is private and may contain Protecte

[Histonet] Help...

[Fawn Bomar]

Does anyone have a protocol or procedure for validating the Her2 antibody?  We 
are trying to get ready for a CAP inspection and I'm not sure if what we did is 
ok or not.

Thank you in advance
Fawn
-
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If you are not an intended recipient of this message, please 
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[Histonet] Cutting Teeth

[Fawn Bomar]

Hi everyone,

I hope you all had A wonderful Holiday.  Does anyone have any suggestions on 
how to soften teeth to cut in paraffin?

Thank you
Fawn
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[Histonet] Need a manual

[Fawn Bomar]

Hi everyone,

I was wondering if anyone has a manual for the Tissue-Tek II Cryostat that they 
would be willing to share.

Thank you
Fawn Bomar, HT(ASCP)
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