[Histonet] Freezing artefact
Good Afternoon all, I have a couple of cases where my post mortem samples have been accidentally frozen in Neutral Buffered Formalin. Naturally this has led to pretty striking artefact in the tissue. I was wondering if anyone who perhaps has more experience of dealing with frozen tissue has any tips on minimising this artefact? I still have some fixed tissue but am unable to resample from the unfixed tissue. Thanks in advance. Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.uk<mailto:finlay.fin...@glasgow.ac.uk> The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Attwood's tips please
Hello, I'm trying to work up an Attwood's method but would be keen to get a guide on time for the differentiation step in tartrazine solution. The method I'm using suggests 20 minutes at the longest with regular microscope checks. I'm currently at about 4 hours with only slight loss of colour in the RBC's. I know that the tartrazine solution will work eventually as the slides I left in over the weekend became fully decolourised but those time scales are unworkable. Thanks, Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.uk<mailto:finlay.fin...@glasgow.ac.uk> The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] training logs
Thank you to everyone who has emailed me example training logs. A few people have requested that I share the results with them and it was suggested that I put up a dropbox link to all of those provided. I thought that this sounded like a good idea so I will do this later, if anyone would rather that I did not include their documents or if anyone else would like to add additional documents to this collection then please let me know today. Thanks again Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.uk<mailto:finlay.fin...@glasgow.ac.uk> The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] training log
Hello all, Does anyone have a training log or competency list that they would be willing to share with me for an absolute beginner to hands on histology. I have previously trained people with limited experience but never from scratch (straight from university). I am keen not to miss the basic lab skills that I've previously taken for granted. Thank you in advance Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.uk<mailto:finlay.fin...@glasgow.ac.uk> The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mortuary scales advice
Hello all, I am searching for a weighing scale that will record multiple organ weights during autopsy and then allow display of all data at the end of the session. Ideally this would be a stand-alone unit rather than computer linked. Does anyone have experience with this sort of equipment? Thank you Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.uk<mailto:finlay.fin...@glasgow.ac.uk> The University of Glasgow Charity Number SC004401 Disclaimer: This e-mail is intended for the recipient only. If you are not the intended recipient you must not use, disclose, distribute, copy, print, or rely upon this e-mail. If an addressing or transmission error has misdirected this e-mail, please notify the author by replying to this e-mail and delete any local copy from your machine. E-mails are not necessarily secure. Forensic Medicine & Science (FMS) does not accept responsibility for changes made to this message after it was sent. Opinions, conclusions and other information in this message expressed by others or which do not relate to the official business of FMS shall be understood as neither given nor endorsed by FMS. It is expressly declared that this e-mail does not constitute nor form part of any contract or unilateral obligation unless the contrary is specifically stated in this e-mail and authorised by FMS. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] wearable formalin monitors
Hello Just wondering what everyone's experience is with different wearable formalin monitors. I'm looking at the AirChek ones from Leica at the moment. Thank you Finlay Finlay Senior Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.uk <mailto:finlay.fin...@glasgow.ac.uk> The University of Glasgow Charity Number SC004401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Leica RM2255 block clamp
Thank you to everyone who took the time to advise me about the block clamp. Looks like I'm going to buy a new clamp and also get out University engineering guys to have a tinker with the old one. Finlay From: histonet-boun...@lists.utsouthwestern.edu on behalf of Finlay Finlay Sent: Tuesday, November 19, 2013 1:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica RM2255 block clamp Hello Hoping someone might be able to help with this microtome issue. I have a problem with the block clamp on one of my Leica RM2255's. It has recently loosened up and it is now possible to move the clamped cassette from side-to-side when the clamp is shut. When I turn the bolt on the underside of the clamp it neither tightens up nor loosens off so there does not seem to be a way of taking the clamp apart any further. Leica have suggested that I buy a new block clamp (£292) but it just feels like something that could be easily fixed, perhaps with a new spring? Has anyone had experience with this problem? Thank you Finlay Finlay Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.uk <mailto:finlay.fin...@glasgow.ac.uk> The University of Glasgow Charity Number SC004401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica RM2255 block clamp
Hello Hoping someone might be able to help with this microtome issue. I have a problem with the block clamp on one of my Leica RM2255's. It has recently loosened up and it is now possible to move the clamped cassette from side-to-side when the clamp is shut. When I turn the bolt on the underside of the clamp it neither tightens up nor loosens off so there does not seem to be a way of taking the clamp apart any further. Leica have suggested that I buy a new block clamp (£292) but it just feels like something that could be easily fixed, perhaps with a new spring? Has anyone had experience with this problem? Thank you Finlay Finlay Histology Technician Forensic Medicine and Science School of Medicine College of Medical, Veterinary and Life Sciences University of Glasgow Joseph Black building Direct Line: +44 (0) 141 3303443 E-mail: finlay.fin...@glasgow.ac.uk <mailto:finlay.fin...@glasgow.ac.uk> The University of Glasgow Charity Number SC004401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC on post mortem tissue
Hello I was just wondering if anyone had a link to any papers discussing the loss of antigenicity with decomposition. I work with forensic tissue which is often quite autolysed. Thanks in advance Finlay ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Armand solution
Hello all, Does anyone know of a supplier of Armand solution in the UK or, failing that, a method for making my own. Thank you Finlay ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ORO trivia
Thanks to everyone who replied. No firm answer. I've told my pathologist that I don't know and may now suggest to one of the trainees that they ask him. Finlay ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Oil Red O trivia
Hello Just wondering if anyone knows the what the 'O' in oil red O stands for. I suspect that it stands for nothing other than O similar to the 'G' in Orange G. One of our pathologists is looking for a tricky stain question to annoy trainees with. Thanks for your help Finlay ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] obesity/cardiac study
Hello histonet this is a call for any advice. I am a purely lab-taught histotech based in the UK and have been studying part-time to enable suitable accreditation and for my final year project am intending to use the large back catalogue of blocks in the forensic lab I work in to study cardiac changes associated with obesity (this is of special interest in the West of Scotland where I work). My intention is to select cases where obesity is judged (by pathologist) to be a primary or secondary factor in cause of death and where other factors (i.e. hypertension) were not indicated in life and compare with an age-matched cohort to asses the correlation between obesity and cardiac changes such as degree of interstitial fibrosis. Initially I intend to take a single case with well developed obesity-linked cardiac changes and do a range of stains (H&E, MSB, EVG, PTAH, a range of trichromes, PSR) to determine which stains to use in the whole study. I would be very grateful to hear from anyone who has undertaken similar work with advice on range of stains/techniques or tips on papers I should read or with any other advice. Also any advice on immuno that I could undertake. Thank you Finlay ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
